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Hyoscyamine-producing marine Actinomycetes from Lagos Lagoon sediment

Asian Pacific Journal of Tropical Biomedicine 2015年第3期5卷 196-201页

作者： Davies Olabisi Flora Adeleye Isaac Adeyemi Wang Peng George Department of Microbiology.University of Lagos Department of Chemistry Georgia State University

Objective:To isolate and screen Actinoniycetes from Lagos Lagoon soil sediments for production of bioactive ***:Sediment samples were collected from four different locations of Lagos Lagoon and were dried for 2 weeks after which the Actinoniycetes were isolated by serial dilution using the spread plate method on starch casein and Kuster's agar supplemented with 80 ug/mL cycloheximide to prevent fungal *** plates were incubated at 28 C for 1-2 *** were selected based on their colonial characteristics as well as their Gram's reaction and subciiltured using the same media for isolation until pure cultures were obtained and incubated at 28 C for 3 ***,they were inoculated into starch casein and Kuster's broth media and incubated for 8 *** secondary metabolites were screened for antimicrobial activity against the following microorganisms:methicillin resistant Staphylococcus *** aureus ATCC *** coli ATCC *** aeruginosa ATCC *** albicans and Enterocolitis faecal is ATCC *** staphylococci isolated from HIV patients were also used(Staphylococcus *** xylosus and Staphylococcus epidennidis).The antimicrobial metabolites of the Actinoniycetes isolates were identified using gas chromatography(GC).Results:Crude extracts of isolates showed antimicrobial activity against some of the test *** GC data analysis showed the antibiotic profile of these ***:Analysis of the crude extracts of the isolates using GC method,revealed the presence of antibiotics including an anticholinergic hyoscyamine among other conclusions.

关键词： Marine Actinomycetes Streptomyces antimethicillin resistance Molecular identification Morphological characteristics Gas chromatography

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