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Changes of cd8+cd28-T regulatory cells in rat model of colitis induced by 2,4-dinitrofluorobenzene

World Journal of Gastroenterology 2003年第11期9卷 2528-2532页

作者： Wen-Bin Xiao, Department of Gastroenterology, Peking University People’s Hospital, Beijing 100044, China Yu-Lan Liu, Department of Gastroenterology, Peking University People’s Hospital, Beijing 100044, China Department of Gastroenterology Peking University People's Hospital Beijing 100044 China.

AIM:To determine the changes of cd8+ T subsets especially cd8+cd28-T regulatory cells in rat model of experimental colitis induced by 2,4-dinitrofluorobenzene (DNFB). METHODS:The rat model of experimental colitis was induced by enema with *** days later,colonic intraepithelial and splenic lymphooltes were isolated from colitis animals (n=16) and controls (n=8).The proportion of cd8+ T cells,cd8+cd28+ T cells and cd8+cd28-T regulatory cells were determined by flow cytometry. RESULTS:The model of experimental colitis was successfully established by DNFB that was demonstrated by bloody diarrhea,weight loss and colonic *** proportion of cd8+ T cells in either splenic or colonic intraepithelial lymphocytes was not significantly different between colitis animals and controls (spleen:34.6±7.24 % vs 33.5±9.41%, colon:14.0±8.93 % vs 18.0±4.06 %,P>0.05).But cd8+cd28- T regulatory cells from colitis animals were significantly more than those from controls (spleen:11.3±2.26 % vs 5.64±1.01%, colon:6.50±5.37 % vs 1.07±0.65 %,Pcd8+cd28+ T cells from colitis animals were less than those from controls (spleen:23.3±6.14 % vs 27.8±9.70 %,P=0.06; colon:7.52±4.18 % vs 16.9±4.07 %,Pcd8+cd28-T regulatory cells in splenic and colon intraepithelial cd8+ T cells from colitis animals was higher than that from controls (spleen:33.3±5.49 % vs 18.4±7.26 %, colon:46.0±14.3 % vs6.10±3.72 %,Pcd8+cd28-T regulatory cells in rats with experimental colitis is increased,which may be associated with the pathogenesis of colitis.

关键词： Animals antigens, cd28 cd8-Positive T-Lymphocytes Colitis Colon Dinitrofluorobenzene Disease Models, Animal Flow Cytometry Male Rats Rats, Sprague-Dawley Research Support, Non-U.S. Gov't Specific Pathogen-Free Organisms Spleen

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