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Isolation of a novel antifungal peptide from the bark of Eucommia ulmoides Oliv

High Technology Letters 2008年第2期14卷 216-219页

作者：刘世会 Zhao Degang Han Yuzhen Guizhou Key Laboratory of Agricultural Bioengineering College of Life Sciences Guizhou University Guiyang 550025 P.R. China Key Laboratory of Green Pesticide and Agricultural Biological Engineering Ministry of Education Guizhou University Guiyang 550025 P.R. China College of Biological Sciences China Agricultural University Beijing 100094 P.R. ChinaI

A novel Eucommia antifungal peptide, named EAFP3, was isolated from the bark of Eucommia ul- moides by NaC1 extract, gel filtration and reverse phase high performance liquid chromatography. The molecular mass of EAFP3 is 4157.3 Da, and its partial amino acid sequence is -. LYQQLIAGITLNK.-. EAFP3 exerts an inhibitory activity against Candida albicans in vitro and the drug concentration required for 50% growth inhibition （IC50） is 31.25μg/mL.

关键词： antifungal peptide isolation the bark of Eucommia ulmoides Oliv Candida albicans

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Purification and Molecular Identification of an antifungal peptide from the Hemolymph of Musca domestica （housefly）

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Cellular & Molecular Immunology 2009年第4期6卷 245-251页

作者： Ping Fu Jianwei Wu Guo Guo Department of Parasitology Guiyang Medical College Guiyang Guizhou550004 China Department of Parasitology GuiyangMedical College 9 Beijing Road Guiyang 550004 China

Antibacterial and antifungal peptides found in houseflies （Musca domestica） in large number are indispensable components of its immune defense mechanism. In this study the anterior tip of the larvae of housefly was cut off with a pair of fine scissors and hemolymph was collected and exuded in an ice-cold test tube. From the hemolymph an antifungal substance was isolated by solid-phase extraction combined with reverse phase-high performance liquid chromotography （RP-HPLC） and named as Musca domestica antifungal peptide-1 （MAF-1）. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis （SDS-PAGE） showed its molecular weight was 17 kDa. UV absorption spectra revealed that this antifungal substance possessed the characteristics of protein peptides. Analysis by fingerprint-identification and tandem mass spectrometry suggested MAF-I was an unknown protein. Edman degradation identified the sequence of 30 amino acids of its N-terminal which matched no peptide in the MASCOT search database, indicating MAF-1 was a novel insect antifungal peptide. Mass spectrometry showed the precise molecular weight of MAF-1 was 17203.384 Da. Its isoelectric point was acidic.

关键词： Musca domestica antifungal peptide purification molecular identification

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Divergence on Gene Expression Responding to Microbial Challenge and the Evolution of the Drosomycin Gene Family in Drosophila melanogaster

Divergence on Gene Expression Responding to Microbial Challe...

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中国蚕学会第六届青年学术研讨会

作者： DENG Xiao-Juan 1,2,YANG Wan-Ying 2,HUANG Ya-Dong3,CAO Yang2,WEN Shuo-Yang 4,XU Pei-Lin 1,XIA Qing-You 5 (1 The Key Laboratory of Gene Engineering of Ministry of Education,Sun Yat-sen University,Guangzhou 510275,China 2 Department of Sericulture Science,College of Animal Science,South China Agricultural University,Guangzhou 510642,China 3 Biopharmaceutical Research and Development Center,Jinan University,Guangzhou 510632,China 4 Department of Entomology,College of Natural Resource and Environment,South China Agricultural University,Guangzhou 510642,China 5 Key Sericultural Laboratory of Agriculture Ministry,College of Sericulture and Biotechnology,Southwest University,Chongqing 400716,China)

Drosomycin（Drs） encodes an inducible 44-residue of antifungal peptide in Drosophila melanogaster. Additional six genes,Dro1,Dro2,Dor3,Dro4,Dro5 and Dro6,are clustered with Drs into a multi-gene family on the 3L chromosome arm. To understand how does each gene of the family respond to microbial infection,we investigated the expression pattern of the drosomycin gene family by microbe-free injury and the microbial challenge. Among these seven genes,Drs,Dro2,Dro3,Dro4 and Dro5,were constitutionally expressed. Dro2,Dro3 and Dro5 were up-regulated by the simple injury. Only the Drs was strongly up-regulated by infection of microbes. Nevertheless Dro1 and Dro6 were not transcribed at all. Furthermore,two transcription start sites of Drs and Dro2,each of Dro3,Dro4 and Dro5,were identified by rapid amplification of cDNA ends. The presence of the NF-κB binding sites in regulatory region of Drs,Dro2,Dro3 and Dro5 genes also indicated the importance of these sites for the inducibility of Drs,Dro2,Dro3 and Dro5 gene. Taking the nested phylogenic information of ORFs into account,we suggested that genes of the drosomycin family have evolved into quite divergent against various stimulations during three duplication events.

关键词： Drosomycins antifungal peptide Transcription Regulatory motifs Drosophila melanogaster

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