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Comparative Analysis of Hydration Layer Reorientation Dynamics of antifreeze protein and protein Cytochrome P450

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Chinese Journal of Chemical Physics 2022年第3期35卷 509-515,I0002,I0003页

作者： Hongfeng Yu Qiang Zhang Wei Zhuang State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China University of Chinese Academy of Sciences Beijing 100049China College of Chemistry and Material Sciences Inner Mongolia Minzu UniversityTongliao 028043China Institute of Urban Environment Chinese Academy of SciencesXiamen 361021China

antifreeze proteins(AFPs)inhibit ice recrystallization by a mechanism remaining largely *** of AFPs’hydration water and its involvement in the antifreeze activity have not been identified *** herein,by simulation and theory,examined the water reorientation dynamics in the first hydration layer of an AFP from the spruce budworm,Choristoneura fumiferana,compared with a protein cytochrome P450(CYP).The increase of potential acceptor water molecules around donor water molecules leads to the acceleration of hydrogen bond exchange between water ***,the jump reorientation of water molecules around the AFP active region is *** to the mutual coupling and excitation of hydrogen bond exchange,with the acceleration of hydrogen bond exchange,the rearrangement of the hydrogen bond network and the frame reorientation of water are ***,the water reorientation dynamics of AFP is faster than that of *** results of this study provide a new physical image of antifreeze protein and a new understanding of the antifreeze mechanism of antifreeze proteins.

关键词： Hydration layer antifreeze protein Reorientation dynamics

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Construction of plant expression vector of Pseudopleuronectes americanus antifreeze protein gene

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Journal of Northeast Agricultural University(English Edition) 2007年第3期14卷 206-211页

作者： LI Shufeng ZHAO Wei YUAN Lili WU Jiang YAN Yunqin College of Life Sciences Northeast Agricultural University Harbin 150030 China

The Pseudopleuronectes americanus antifreeze protein gene was synthesized and control sequences were added such as 35S promoter and nos terminator that can facilitate the transcription and Ω sequence and Kozak sequence that can improve the expression in translation level, the high expression cassette of antifreeze protein was constructed. This cassette was connected to pBI121.1 and finally got the high expression vector pBRTSAFP introduced into the maize callus. The expression of gus gene that linked to the antifreeze protein gene was detected, and the results was that the gus gene can express strongly and instantaneously.

关键词： vector construction antifreeze protein control sequence

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Sequencing and expression characterization of antifreeze protein Maxi-like in Apis cerana cerana

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Sequencing and expression characterization of antifreeze pro...

Transcriptomic and proteomic analyses on the supercooling ability and mining of antifreeze proteins of the Chinese white wax scale insect

21世纪第三届全国蜂业科技与海峡两岸蜂产业发展大会暨首届北京密云蜂产业发展高峰论坛

作者：徐凯吉林省养蜂科学研究所

antifreeze proteins(AFPs) are biological cryoprotectants with unique properties that play a crucial role in regulating the molecular mechanisms governing cold resistance in *** identify and characterize Apiscerana cerana AFP(AcerAFP),we cloned the full-length cDNA of AcerA FP and examined its expression patterns in *** cerana.A nucleotide alignment analysis showed that the entire coding region of AcerAFP is 1095 bp and encodes a polypeptide of 365 amino *** amino acid sequence of this protein exhibits 63% to 96% homology with AFP homologs from other ***-helices form the main secondary and tertiary structures of AcerAFP,which is similar to the molecular structure of fish AFP *** expression profiles of AcerAFP revealed that expression was tissue,sex,and developmentally *** response to cold stress,the mRNA and protein expression of AcerA FP were both induced by low temperatures,and were also related to the concentrations of several cryoprotectants,including glucose,glycerin,glutamic acid,cysteine,histidine,alanine,and *** addition,we found that the knockdown of AcerAFP by RNA interference remarkably increased the total freezing temperature of hemolymph in Apis cerana cerana,where levels of AcerAFP mRNA were correlated with the expression of most antifreeze-related *** together,these results suggest that AcerAFP plays an essential role as a biological cryoprotectant in honeybees,and is in turn regulated by small cryoprotectants and antifreeze-related proteins.

关键词： antifreeze protein Apis cerana cerana cold stress expression patterns RNA interference

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Insect Science 2016年第3期23卷 430-437页

作者： Shu-Hui Yu Pu Yang Tao Sun Qian Qi Xue-Qing Wang Xiao-Ming Chen Ying Feng Bo-Wen Liu Research Institute of Resources Insects Chinese Academy of Forestry Key Laboratory of Cultivating and Utilization of Resources Insectsof State Forestry Administration Kunming China

The Chinese white wax scale insect, Ericerus pela, can survive at extremely low temperatures, and some overwintering individuals exhibit supercooling at tempera- tures below -30℃. To investigate the deep supercooling ability ofE. pela, transcriptomic and proteomic analyses were performed to delineate the major gene and protein families responsible for the deep supercooling ability of overwintering females. Gene Ontology （GO） classification and Kyoto Encyclopedia of Genes and Genomes （KEGG） analysis indicated that genes involved in the mitogen-activated protein kinase, calcium, and PI3K-Akt signaling pathways and pathways associated with the biosynthesis of soluble sugars, sugar alcohols and free amino acids were dominant. proteins responsible for low-temperature stress, such as cold acclimation proteins, glycerol biosynthesis-related enzymes and heat shock proteins （HSPs） were identified. However, no antifreeze proteins （AFPs） were identified through sequence similarity search methods. A random forest approach identified 388 putative AFPs in the proteome. The AFP gene ep-afp was expressed in Escherichia coli, and the expressed protein exhibited a thermal hysteresis activity of 0.97℃, suggesting its potential role in the deep supercooling ability ofE. pela.

关键词： antifreeze protein Chinese white wax scale insect proteome, supercoiling thermal hysteresis activity transcriptome

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昆虫抗冻机理研究进展(英文)

Insect Science 1997年第3期 265-276页

作者：赵章武中国科学院动物研究所!北京100080

大多数冰冻耐受性昆虫具有蛋白质／脂蛋白质或非溶性的晶体，它们相对地在较高温度下具有激活体内冰核的作用。最近已确证，许多昆虫肠道中正常的细菌和真菌是冰核激活菌丛。而对于非冰冻耐受性的昆虫，其存活是不允许体内冰的形成。它... 详细信息

大多数冰冻耐受性昆虫具有蛋白质／脂蛋白质或非溶性的晶体，它们相对地在较高温度下具有激活体内冰核的作用。最近已确证，许多昆虫肠道中正常的细菌和真菌是冰核激活菌丛。而对于非冰冻耐受性的昆虫，其存活是不允许体内冰的形成。它们在过冬过程中，关键是要调节体液的过冷却点，避免结冰。为了增加抗冻能力，非冰冻耐受性的过冬昆虫通过去除内源性冰核、积累低分子量的多元醇和糖类以及血淋巴中抗冻蛋白或抗冻肽的合成来降低体液的过冷却点。本文详尽综述了过冬昆虫抗冻机理的研究进展。

关键词： insect cold-hardiness antifreeze protein ice nucleating active agents