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Antifeedant Activity of Caesalpinia coriaria Essential Oil Against Incisitermes marginipennis (Latreille)

Phyton-International Journal of Experimental Botany 2021年第3期90卷 907-920页

作者： Cesar B.Ramírez-López Rita Beltrán-Sánchez Alistair Hernández-Izquierdo JoséLuis Salvador-Hernández Eduardo Salcedo-Pérez Rosa E.del Río Mauro M.Martínez Pacheco Instituto de Investigaciones Químico Biológicas Universidad Michoacana de San Nicolás de HidalgoMorelia58030Mexico Departamento de Botánica y Zoología CUCBA Universidad de GuadalajaraNextipac45200Mexico

This study scrutinized the possibility of finding toxicant or deterrent plant metabolites against the dry wood termite Incisitermes marginipennis(Latreille).Plant deterrent agents act as repellents or Antifeedants to prevent wood decay and increase its useful life.The potential of the tree Caesalpinia coriaria(Fabaceae)as a biological source of molecules with deterrent effects against the dry wood termite was assessed by a phytochemical fractionation guided by repellence and Antifeedant activities.The gas chromatography-mass spectrometry analysis of the leaf essential oil showed geraniol to be one of the major components and its repellent and Antifeedant effects were determined.Geraniol had only an Antifeedant effect without affecting the body weight or survival of the dry wood termite.Unlike the leaf essential oil,geraniol did not exhibit a repellency effect.An in-silico approach of the activity of acetylcholinesterase in interaction with geraniol resulted in an affinity energy of−7.5 Kcal/mol.Geraniol interacted with the amino acid tyrosine 324 located in the enzyme’s active site while citronellol(negative control)interacted with tryptophan 83 located adjacent to the active site.These deterring terpenes have not been implemented for the preservation and restoration of wood products exposed to the attack of the dry wood termite.However,they are an important natural control alternative.

关键词： Termite geraniol repellent Antifeedant deterrent

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Antimicrobial,herbicidal and Antifeedant activities of mansonone E from the heartwoods of Mansonia gagei Drumm

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Journal of Integrative Agriculture 2016年第12期15卷 2795-2802页

作者： Rachsawan Mongkol Warinthorn Chavasiri Program in Biotechnology Faculty of ScienceChulalongkorn University Natural Products Research Unit Department of ChemistryFaculty of ScienceChulalongkorn University

In vitro biological activities including anti-phytopathogenic fungi, antibacterial, Antifeedant and herbicidal activities of the extracts from the heartwoods of Mansonia gagei Drumm. were evaluated. The dichloromethane （DCM） extract displayed antifungal activity against four plant pathogenic fungi （Altemaria porri, Colletotrichum gloeosporioides, Fusarium oxysporum and Phytophthora parasitica） higher than the methanolic （MeOH） extract. The separation of the DCM extract using bioassay guided antifungal activity against P. parasiUca led to the isolation of mansorins A, B, and C, mansonones C, E, G and H. Among isolated compounds, mansonone E displayed the highest antifungal activity against P. parasitica, followed by mansonone C, mansorin B and mansonone G. This potent compound revealed the same minimum inhibitory concentrations （MIC） of 31 μg mL-1 against C. gloeosporioides and P. parasitica, and minimum fungicidal concentration （MFC） of 31 and 125 μg mL-1, respectively. Moreover, mansonone E exhibited highly significant antibacterial activity against both Xanthomonas oryzae pv. oryzae （Xoo） and X. oryzae pv. oryzicola （Xoc） with MIC and minimum bactericidal concentration （MBC） as 7.8 and 〉500μg mL-1, respectively. This compound furthermore could inhibit the feed of Spodoptera litura with 45.9% Antifeedant and significantly herbicidal activity reduced the shoot and root growth of Brassica chinensis, Oryza sativa, Mimosa pigra and Echinochlooa crus-galli. Mansonone E has potential as a new natural pesticide for agricultural plant pathogen management.

关键词： Mansonia gagei Drumm. mansonone E plant pathogens antifungal antibacterial Antifeedant herbicidal activities

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Identification, isolation and characterization of the Antifeedant constituent of Clausena anisata against Helicoverpa armigera （Lepidoptera： Noctuidae）

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Insect Science 2009年第3期16卷 247-253页

作者： Olufemi O. R. Pitan O. O. Ayelaagbe Hong-Lei Wang Chen-Zhu Wang State Key Laboratory of Integrated Management of Pest insects and Rodents Institute of Zoology Chinese Academy of Sciences BeijingChina Department of Crop Protection University of Agriculture Abeokuta Department of Chemistry University of Ibadan IbadanNigeria

Hexane, petroleum ether, chloroform, ethyl acetate, methanol and water extracts of Clausena anisata [（Willd.） Hook E Ex Benth] leaves and roots were evaluated against Helicoverpa armigera （Hubner） for Antifeedant activities. Antifeedant activity was confirmed, and was found to be higher in root extracts than those of the leaf. Chloroform and petroleum ether extracts of the root showed strongest Antifeedant activities （DC50s [concentration （C） causing 50% deterrence compared with the control] 0.014% and 0.016% respectively）, and root extracts were fractionated using silica gel column chromatography. One fraction of the chloroform and one of the petroleum ether root extracts was active; and on the basis of mass spectroscopy and ^1H and ^13C nuclear magnetic resonance spectral data, the active compounds in the two fractions were confirmed to be identical, and identified as osthol [2H-1-Benzopyran-2-one, 7-methoxy-8-（3-methyl-2-butenyl）]. The highest concentration of osthol was found in the chloroform root extract. Antifeedant activities of the root extracts, as measured by DC50 values, were highly correlated with their osthol contents. Approximately 99% of the variation in bioactivity of the root extracts could be accounted for by variation in osthol content; osthol therefore, appeared to be an Antifeedant component of C. anisata to H. armigera. This may provide a new approach to managing this pest.

关键词： active ingredient Antifeedant Clausena anisata Helicoverpa armigera,osthol root

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Antibacterial and Antifeedant activities of Spilanthes acmella leaf extract against Gram-negative and Gram-positive bacteria and brinjal fruit borer,Leucinodes orbonalis larvae

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Journal of Coastal Life Medicine 2014年第12期2卷 980-985页

作者： Manickam Pavunraj Kathirvelu Baskar Sundaram Janarthanan Munusamy Arumugam PG&Research Department of Zoology Vivekananda CollegeThiruvedakam WestMadurai-625217India Bioscience Research Foundation PorurChennai-600116Tamil NaduIndia Department of Zoology University of MadrasChennai-600025Tamil NaduIndia

Objective:To evaluate the antibacterial and Antifeedant activities of dichloromethane,acetone and aqueous extracts of Spilanthes acmella(L.)(S.acmella)Murr against selected bacterial strains and larvae of Leucinodes orbonalis Guen.Methods:Solvent extracts were tested against pathogenic microbes using disc diffusion method and fruit disc no-choice method for Antifeedant activity.Results:The study revealed that dichloromethane extract of S.acmella leaf showed broad spectrum antibacterial activity against all the tested bacteria.Maximum zone of inhibition was observed in dichloromethane leaf extract of S.acmella against Escherichia coli[(18.9±0.34)mm]followed by Staphylococcus aureus[(18.6±1.31)mm],Proteus vulgaris[(17.2±0.68)mm],Bacillus subtilis[(17.0±0.76)mm]and Klebsiella pneumonia[(16.4±1.55)mm]at 5 mg/disc concentration.The aqueous extract was moderately inhibited the tested bacteria at all the concentrations.Dichloromethane extract showed good Antifeedant activity against Leucinodes orbonalis(68.88%)when compared to acetone(60.80%)and aqueous(45.48%)extracts at 5%concentration.The preliminary phytochemical analysis showed the presence of alkaloids,terpenoids,phytosterols,saponins,steroid,tannins and phenolic compounds.Conclusions:The study suggests that the dichloromethane leaf extract of S.acmella could be used to develop a novel herbal formulation to control pathogenic bacteria and agricultural pests.

关键词： Antibacterrial Antifeedant Spilanthes acmella Leucinodes orbonalis

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Protection effect of the anthraquinones, cassiatorin and aurantio-obtusin from seeds of Senna tora against cowpea weevil attack

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Asian Pacific Journal of Tropical Biomedicine 2018年第2期8卷 98-105页

作者： Valentine Chi Mbatchou Rita Akosua Dickson Isaac Kingsley Amponsah Abraham Yeboah Mensah Solomon Habtemariam Department of Applied Chemistry and Biochemistry University for Development Studies Navrongo Campus Department of Pharmacognosy Faculty of Pharmacy and Pharmaceutical Sciences Kwame Nkrumah University of Science and Technology Pharmacognosy Research Laboratories & Herbal Analysis Services UK University of Greenwich

Objetive: To explore the potential insecticidal, ovipositor deterrent and Antifeedant effects of ethyl acetate extract of the seeds of Senna tora(Syn. Cassia tora) against cowpea weevil(Callosobruchus maculatus).Method: The activities were evaluated using standard protocols.In these bioassays, the cowpea seeds were used directly as an insect feed. The activity of the extract and isolated compounds were tested at concentrations of 100, 200 and 300 μg/mL and compared to neem oil and cinnamaldehyde(as standard positive controls). Phytochemical analysis of the ethyl acetate extract was done through a number of chromatographic techniques and the structures of the isolated compounds were established through comprehensive spectroscopic analysis including 2 D-NMR and ESI-MS studies.Results: Fractionation of the active ethyl acetate extract resulted in the isolation of one known anthraquinone,aurantio-obtusin(1) and a novel compound that was named as cassiatorin(2). Compounds1 and 2 showed comparable insect Antifeedant properties with the positive controls while their insecticidal and ovipositor deterrent effects were far superior to the standard controls.Conclusions: It is thus concluded that Senna tora extracts and the isolated compounds(1 and2) may be employed in the postharvest management of stored cowpea seeds and as other crop protectants.

关键词： Senna tora Cassia tora Cassiatorin Aurantio-obtusin Insecticidal Ovipositor deterrent Antifeedant Cowpea weevil

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Synthesis and Bio-activities of Pyrazolyl or Pyridinyl Substituted Tonghaosu Analogs

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Chinese Journal of Chemistry 2007年第6期25卷 808-813页

作者：尹标林陈立徐汉虹张健存吴毓林 Guangzhou Institute of Biomedicine and Health Chinese Academy of Science Guangzhou Guangong 510663 China State Key Laboratory ofBio-organic & Natural Products Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China Laboratory of Insect Toxicology South China Agricultural University Guangzhou Guangdong 510642 China

11 pyrazolyl or pyridinyl substituted tonghaosu analogs were synthesized. Structures of all the new compounds were confirmed by ^1H NMR, IR, MS, HREIMS or elemental analysis. Their Antifeedant activity against larvae of large white butterfly （Pieris brassicae L.）, larvicidal activity toward mosquito （Culex quinquefasciatus Say） and growth control activity toward larvae of Spodoptera litura Fab were examined. Some of them exhibited antifeeding activities comparable to or stronger than tonghaosu Z-1. Based on the activity data, the preliminary structure-activity relationship was also discussed, which might be instructive for finding out lead compounds with better bioactivities in the future.

关键词： spiroketal tonghaosu analog Antifeedant Larvicidal growth control Pieris brassicae Culex quinquefasciatus Spodoptera litura

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Phytopesticidal effects of Spilanthes acmella(L.)Murr.leaves on three economically important lepidopteran insect pests

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Journal of Coastal Life Medicine 2014年第7期2卷 549-554页

作者： Manickam Pavunraj Kathirvelu Baskar Sundaram Janarthanan Munusamy Arumugam Department of Zoology University of MadrasChennai-600025Tamil NaduIndia Entomology Research Institute Loyola CollegeChennai-600034Tamil NaduIndia

Objective:To evaluate the Antifeedant and larvicidal activities of dichloromethane(DCM),acetone,dimethylsulfoxide(DMSO)and aqueous extracts of Spilanthes acmella(S.acmella)leaves against Earias vitella,Helicoverpa armigera and Spodoptera litura.Methods:DCM,acetone,DMSO and aqueous extracts were studied using fruit and leaf discs no-choice method at 0.625%,1.25%,2.5%and 5%concentration.Results:All the extracts showed Antifeedant and larvicidal activities against all the tested insects.However,maximum Antifeedant activity was detected in DCM extract of S.acmella against all tested pest,followed by acetone,DMSO and aqueous extracts at 5%concentration.The leaves extract of S.acmella also showed larvicidal activity;maximum larval mortality was observed in DCM extract against Helicoverpa armigera(48.88%),Spodoptera litura(44.88%)and Earias vitella(75.11%)followed by acetone,DMSO and aqueous extracts at 5%concentration.Conclusions:DCM extract of S.acmella could be further purified to develop a formulation for eco-friendly pest control agent.

关键词： Spilanthes acmella Antifeedant Larvicidal Lepidopteran

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Highly Oxygenated Limonoids and Lignans from Phyllanthus flexuosus

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Natural Products and Bioprospecting 2014年第4期4卷 233-242页

作者： Jian-Qiang Zhao Yan-Ming Wang Hong-Tao Zhu Dong Wang Sheng-Hong Li Rong-Rong Cheng Chong-Ren Yang Yi-Fei Wang Min Xu Ying-Jun Zhang State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201People’s Republic of China University of Chinese Academy of Sciences Beijing 100049People’s Republic of China Guangzhou Jinan Biomedicine Research and Development Center Guangzhou 510632People’s Republic of China

Two new highly oxygenated limonoids,flexuosoids A(1)and B(2),and three new arylnaphthalene lignan glycosides,phyllanthusmins D–F(3–5),were isolated from the roots of Phyllanthus flexuosus,in addition to three known lignans,phyllanthusmin C,arabelline,and(?)-diasyringaresinol.Their structures were elucidated on the basis of detailed spectroscopic analysis and chemical methods.Compounds 1 and 2,two new decaoxygenated limonoids with a C-19/29 lactol bridge and heptaoxygenated substituents at C-1,C-2,C-3,C-7,C-11,C-17,and C-30,represent the second example of limonoids in the Euphorbiaceae family.Most of the isolates were tested for their Antifeedant,anti-herpes simplex virus 1,and cytotoxic activities.The new limonoids 1 and 2 showed promising Antifeedant activity against the beet army worm(Spodoptera exigua)with EC50 values of 25.1 and 17.3 lg/cm2,respectively.In addition,both of them displayed moderate cytotoxicity against the ECA109 human esophagus cancer cell line,along with the known lignan glycoside,phyllanthusmin C,with the IC50 values of 11.5(1),8.5(2),and 7.8(phyllanthusmin C)lM,respectively.

关键词： Phyllanthus flexuosus Euphorbiaceae Limonoids Lignan glycosides Antifeedant Antiviral Cytotoxicity

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Bioactivity of Pseudocalymma alliaceum(Lam.)Sandwith(Bignoniaceae)against Spodoptera litura Fabricius and Helicoverpa armigera Hübner(Lepidotera:Noctuidae)

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Journal of Coastal Life Medicine 2014年第4期2卷 302-307页

作者： Alagarmalai Jeyasankar Tamilarasu Chinnamani Entomology Lab PG&Research Department of ZoologyArignar Anna Government Arts CollegeMusiri-621211Tamil NaduIndia

Objective:To evaluate the Antifeedant,larvicidal and insect growth inhibitory activities of crude extracts of Pseudocalymma alliaceum tested against fourth instar larvae of Spodoptera litura(S.litura)and Helicoverpa armigera(H.armigera).Methods:Hexane,chloroform and ethyl acetate extracts were prepared and tested for Antifeedant,insecticidal and growth inhibitory activities against fourth instar larvae of S.litura and H.armigera.Results:Ethyl acetate extract showed promising Antifeedant,insecticidal activity against S.litura and H.armigera.Maximum percentage of deformed larvae,pupae and adults were found on treatment with ethyl acetate extract.Percentage of successful adult emergence was deteriorated by extract treated larvae.Ethyl acetate extracts of Pseudocalymma alliaceum,showed higher percentage of Antifeedant,insecticidal and growth inhibition activities.Conclusions:This is the first report on S.litura and H.armigera.Further,the active compounds isolated from the ethyl acetate extracts will be useful for controlling economically important insect pests.

关键词： Pseudocalymma alliaceum Helicoverpa armigera Spodoptera litura Antifeedant Insecticidal

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Biological activities of Solanum pseudocapsicum(Solanaceae)against cotton bollworm,Helicoverpa armigera Hübner and armyworm,Spodoptera litura Fabricius(Lepidotera:Noctuidae)

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Asian Pacific Journal of Tropical Biomedicine 2012年第12期2卷 981-986页

作者： Alagarmalai Jeyasankar Selvaraj Premalatha Kuppusamy Elumalai Department of Zoology & Botany Arignar Anna Government Arts College Entomotoxicity Unit.Department of Zoology Government Arts College

Objective:To evaluate the Antifeedant,insecticidal and growth inhibition activities of Solanum pseudocapsicum(S.pseudocapsicum)seed extracts against Spodoptera litura(S.litura)and Helicotverpa armigera(H.armigera).Methods:Hcxane,diethyl cther,dichloromethanc and ethyl acetate seed extracts were prepared and tested for autifeedant,insecticidal and growth inhibitory activities against fourth instar larvae of&litura and H.armigera.Results:Ethyl acetate extract showed promising Antifeedant and insecticidal activities against S.litura and H.armigera.Percentage of deformed larvae,pupae and adults were maximum in treatment of ethyl acetate extract.Percentage of successful adult emergence was deteriorated by seeds on extract treated larvae.Conclusions:Ethyl acetate extracts of S.pseudocapsicum,showed higher efficiency of Antifeedant,insecticidal and growth inhibition activities.Hence,it can be used to controll agricultural insect pests,S.litura and H.armigera.

关键词： Solanum pseudocapsicum Helicoverpa armigera Spodoptera litura Antifeedant Insecticidal

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