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Incidence of toxoplasmosis in patients with cirrhosis

World Journal of Gastroenterology 2004年第3期10卷 452-454页

作者： Sebnem Ustun Umit Aksoy Hande Dagci Galip Ersoz Department of Gastroenterology School of MedicineUniversity of Ege35100 BornovaIzmirTurkey Department of Parasitology School of MedicineUniversity of Dokuz EylulIzmirTurkey Department of Parasitology School of MedicineUniversity of EgeIzmirTurkey Department of Gastroenterology School of MedicineUniversity of EgeIzmirTurkey

AIM:It is known that toxoplasmosis rarely leads to various liver pathologies,most common of which is granulomatose hepatitis in patients having normal immune *** who have cirrhosis of the liver are subject to a variety of cellular as well as humoral immunity ***,it may be considered that toxoplasmosis can cause more frequent and more severe diseases in patients with cirrhosis and is capable of changing the course of the *** aim of this study was to investigate the frequency of toxoplasmosis in patients with cirrhosis. METHODS:Serum samples were taken from 108 patients with cirrhosis under observation in the Hepatology Polyclinic of the Gastroenterology Clinic,and a control group made up of 50 healthy blood *** and ELISA methods were used to investigate the IgG and IgM antibodies,which had developed from these sera. RESULTS:Toxoplasma IgG and IgN antibody positivity was found in 74 (68.5%) of the 108 cirrhotic patients and 24 (48%) of the 50 people in the control *** difference between them was significant (P<0.05). CONCLUSION:In conclusion,it was found that the toxopiasma sero-prevalence in the cirrhotic patients in this study was *** patients are likely to form a toxoplasma risk *** detailed studies are needed on this subject.

关键词： Adult Animals antibodies, protozoan Female Humans Incidence Liver Cirrhosis Male Middle Aged Toxoplasma Toxoplasmosis

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Protective effect of DNA-mediated immunization with a combination of SAG1 and IL-2 gene adjuvant against infection of Toxoplasma gondii in mice

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Chinese Medical Journal 2002年第10期115卷 1448-1452页

作者：陈观今陈海峰郭虹郑焕钦汪琦中山大学中山医学院寄生虫学研究所广州510089

OBJECTIVE: To characterize the immune response induced by SAG1 encoding plasmid combined with IL-2 gene adjuvant in mice and to assess the protective effect of this vaccination against toxoplasmosis. METHODS: Mice were co-injected intramuscularly with plasmid encoding Toxoplasma gondii SAG1 plus murine IL-2 expression vector at a dose of 100 microg. Booster immunizations were employed 2 more times at 3-week interval. As controls, mice were inoculated with PBS or empty plasmid pcDNA3. Humoral and cellular responses were assayed using ELISA for the determination of Ab, Ab isotype and IFN-gamma, as well as IL-4. To detect the integration and dissemination of DNA in the injected mice, PCR and in situ hybridization were performed. All mice were then infected with highly virulent RH tachyzoites of Toxoplasma gondii intraperitoneally. RESULTS: Significant increases in specific IgG levels were observed in mice after immunization three times with SAG1 expression plasmid. With respect to the IgG isotype, co-inoculation of IL-2 expression plasmid enhanced the level of IgG2a and the production of IFN-gamma. Challenging mice by vaccinating with combined plasmids with RH tachyzoites resulted in prolonged survival. CONCLUSION: Humoral and cytokine responses elicited by SAG1 DNA immunization can be modulated by co-inoculation with IL-2 expression plasmid. The use of DNA vaccine in combination with an appropriate cytokine gene to prevent T. gondii infection warrants further investigation.

关键词： Antigens, protozoan Animals antibodies, protozoan Cytokines Female Immunization Immunoglobulin G Interleukin-2 Mice protozoan Proteins protozoan Vaccines Research Support, Non-U.S. Gov't Toxoplasma Toxoplasmosis, Animal Vaccines, DNA

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