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A combined evaluation of the characteristics and antibiotic resistance induction potential of antibiotic wastewater during the treatment process

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Journal of Environmental Sciences 2024年第4期138卷 626-636页

作者： Sun Miao Yanyan Zhang Cong Men Yiou Mao Jiane Zuo State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China School of Energy and Environmental Engineering Beijing Key Laboratory of Resource-Oriented Treatment of Industrial PollutantsUniversity of Science and Technology BeijingBeijing 100083China High School Affiliated to Renmin University of China Beijing 100080China Tsinghua Shenzhen International Graduate School Shenzhen 518055China

antibiotic wastewater contains a variety of pollutant stressors that can induce and promote antibiotic resistance(AR)when released into the environment.Although these substances are mostly in concentrations lower than those known to induce AR individually,it is possible that antibiotic wastewater discharge might still promote the AR transmission risk via additive or synergistic effects.However,the comprehensive effect of antibiotic wastewater on AR development has rarely been evaluated,and its treatment efficiency remains unknown.Here,samples were collected from different stages of a cephalosporin production wastewater treatment plant,and the potential AR induction effect of their chemical mixtures was explored through the exposure of the antibiotic-sensitive Escherichia coli K12 strain.Incubation with raw cephalosporin production wastewater significantly promoted mutation rates(3.6×10^(3)-9.3×10^(3)-fold)and minimum inhibition concentrations(6.0-6.7-fold)of E.coli against ampicillin and chloramphenicol.This may be attributed to the inhibition effect and oxidative stress of cephalosporin wastewater on E.coli.The AR induction effect of cephalosporin wastewater decreased after the coagulation sedimentation treatment and was completely removed after the full treatment process.A Pearson correlation analysis revealed that the reduction in the AR induction effect had a strong positive correlation with the removal of organics and biological toxicity.This indicates that the antibiotic wastewater treatment had a collaborative processing effect of conventional pollutants,toxicity,and the AR induction effect.This study illustrates the potential AR transmission risk of antibiotic wastewater and highlights the need for its adequate treatment.

关键词： antibiotic wastewater treatment plant antibiotic resistance antibiotic resistance induction effect Correlation analysis Biological toxicity

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antibiotic resistance Profile of Serotypes of Streptococcus pneumoniae Strains in Bangui, from 2017 to 2022: Case of Serotype 1

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Open Journal of Medical Microbiology 2024年第2期14卷 131-145页

作者： Zéphirin Dalengat Vogbia Ernest Lango Yaya Marceline Djeintote Stéphanie Judith N’Yetobouko Jean de Dieu Longo Clotaire Donatien Rafaï Christian Diamant Mossoro-Kpindet Gérard Gresenguet Epidemiology and Research Service National Reference Center for Sexually Transmitted Infections and Anti-Retroviral Therapy Ministry of Health Bangui Central African Republic Faculty of Health Sciences Doctoral School of Human and Veterinary Health Sciences University of Bangui Bangui Central African Republic National Laboratory of Clinical Biology and Public Health Bangui Central African Republic National Blood Transfusion Center Bangui Central African Republic Public Department Ministry of Health Bangui Central African Republic

Goals: The aim of this study was to determine the antibiotic resistance profile of serotypes of Streptococcus pneumoniae strains circulating in Bangui. Methodology: A prospective and analytical analysis was carried out at the National Laboratory of Clinical Biology and Public Health from 2017 to 2022. The strains came from our study on the contribution to the study of antibiotic sensitivity of Streptococcus pneumoniae strains. The multiplex PCR test was used for its cost-effectiveness in terms of amplifiers which can be purified in order to be sequenced. It also makes it possible to detect several germs as well as their serotypes. For a PCR reaction, several elements are involved in the reaction medium or Master Mix. These are the desoxyribonucleotides (dNTPs), the magnesium ions (MgCl2) and the primers. A set of 14 primers divided into 3 classes were used. Class 1 primers served as an internal control by targeting the cpsA gene. It is a highly conserved gene found in capsular loci characterized to date. The primers of the second class were used to target specific serotypes by specific reactions (out of six possibilities). The group reaction was carried out using the primers of the third class in order to carry out an initial screening of the samples and to classify the pneumococcal isolates. Related serotypes were grouped based on the amplification of common genes. Using the technique of electrophoresis on agarose gel and an ultraviolet radiation device, the migration bands are then visualized and analyzed. The data collected had been entered into Excel 2010 and analyzed with Epi info 7. The exact Fischer chi2 test at the 5% threshold, the relative risk and its 95% confidence interval were used to compare the proportions and determine the associations. Results: 187 antibiotic-resistant strains of Streptococcus pneumoniae were collected. The average frequency of serotypes 1, 9A, 4 and untypeable identified were 43.59%, 18.18%, 18.27% and 39.57% respectively. The frequency of serotype 1 was predominant for the age

关键词： Streptococcus pneumonia Serotype antibiotic resistance Bangui

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Nature’s Pharmacy under Siege: Investigating antibiotic resistance Pattern in Endophytic Bacteria of Medicinal Plants

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Advances in Microbiology 2024年第4期14卷 183-208页

作者： Bonoshree Sarkar Afroza Sultana Nabila Nawar Binti Farhana Tasnim Chowdhury Sadia Afrin Mohammad Fahim Taibur Rahman Atiqur Rahman Infection Biology Laboratory Department of Biochemistry and Molecular Biology University of Dhaka Dhaka Bangladesh Department of Pathology University of Alabama at Birmingham Birmingham USA

antibiotic resistance poses a significant global health threat, necessitating a thorough understanding of its prevalence in various ecological contexts. Medicinal plants, renowned for their therapeutic properties, host endophytic bacteria that produce bioactive compounds. Understanding antibiotic resistance dynamics in these bacteria is vital for human health and antibiotic efficacy preservation. In this study, we investigated antibiotic resistance profiles in endophytic bacteria from five medicinal plants: Thankuni, Neem, Aparajita, Joba, and Snake plant. We isolated and characterized 113 endophytic bacteria, with varying resistance patterns observed against multiple antibiotics. Notably, 53 strains were multidrug-resistant (MDR), with 14 exhibiting extensive drug resistance (XDR). Thankuni-associated bacteria displayed 44% MDR and 11% XDR, while Neem-associated bacteria showed higher resistance (60% MDR, 13% XDR). Aparajita-associated bacteria had lower resistance (22% MDR, 6% XDR), whereas Joba-associated bacteria exhibited substantial resistance (54% MDR, 14% XDR). Snake plant-associated bacteria showed 7% MDR and 4% XDR. Genus-specific distribution revealed Bacillus (47%), Staphylococcus (21%), and Klebsiella (11%) as major contributors to MDR. Our findings highlight diverse drug resistance patterns among plant-associated bacteria and underscore the complexity of antibiotic resistance dynamics in diverse plant environments. Identification of XDR strains emphasizes the severity of the antibiotic resistance problem, warranting further investigation into contributing factors.

关键词： antibiotic resistance Endophytic Bacteria Medicinal Plants Drug resistance

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antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital

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Asian Pacific Journal of Tropical Medicine 2023年第5期16卷 220-231页

作者： Nor Syaza Syahirah Amat Junaidi Nik Noorul Shakira Mohamed Shakrin Mohammad Fahmi Daman Huri Ahmad Zakwan Kamarudin Mohd Nasir Mohd Desa Wan Mohd Zin Wan Yunus Faculty of Medicine and Defence Health Universiti Pertahanan Nasional Malaysia(UPNM)Kem Sungai Besi57000 Sungai BesiKuala LumpurMalaysia Centre for Tropicalization(CENTROP) Universiti Pertahanan Nasional Malaysia(UPNM)Kem Sungai Besi57000 Sungai BesiKuala LumpurMalaysia Pathology Department Tuanku Mizan Armed Forces Hospital(TMAFH)53300 Kuala LumpurMalaysia Faculty of Medicine and Health Sciences Universiti Sains Islam Malaysia(USIM)Persiaran IlmuPutra Nilai71800 NilaiNegeri SembilanMalaysia Department of Biomedical Science Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Faculty of Defence Science and Technology Universiti Pertahanan Nasional Malaysia(UPNM)Kem Sungai Besi57000 Sungai BesiKuala LumpurMalaysia

Objective:To determine the antibiotic resistance profile(ARP)of Staphylococcus(S.)aureus isolates and molecular typing of the methicillin-resistant S.aureus(MRSA)isolates from Tuanku Mizan Armed Forces Hospital(TMAFH),Kuala Lumpur.Methods:The ARP and presence of the pvl gene were determined for 209 S.aureus isolates from clinical specimens.Of these,123 were methicillin-susceptible S.aureus(MSSA)isolates and 86 were MRSA isolates.All MRSA isolates were characterized using SCCmec typing and spa typing.Descriptive analysis was performed to compare the demographic data with the phenotypic and genotypic variables of the S.aureus isolates.Results:No vancomycin-intermediate and-resistant S.aureus(VISA and VRSA,respectively)were detected among the study isolates.The MSSA isolates showed low resistance rates to all tested antibiotics,were commonly invasive(28/42,66.7%),and mostly harboured pvl(35/42,83.3%).Meanwhile,MRSA isolates showed high resistance to penicillin(86/86,100%),ampicillin(86/86,100%),sulbactam/ampicillin(86/86,100%),cefuroxime(81/86,94.19%),cefoperazone(76/86,88.37%),azithromycin(56/86,65.12%),and erythromycin(54/86,62.79%).The majority of MRSA isolates were of SCCmec type IVh(65/86,75.58%),spa type t032(55/85,63.95%),and grouped into spaCC-t022(66/85,77.65%).The t032 type was found to be associated with resistance traits to azithromycin and erythromycin(P<0.05).We also found several spa types that are typically associated with hospital-,community-,and livestock-associated MRSA co-existing in our MRSA population.Conclusions:This study reflected the consistent absence of VISA and VRSA and corroborated the clonal shifting of MRSA isolates in the Malaysian MRSA isolates.

关键词： Staphylococcus aureus Methicillin-resistant Staphylococcus aureus spa typing SCCmec typing antibiotic resistance

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antibiotic resistance pattern of Pseudomonas aeruginosa wound isolates among Chinese burn patients:A systematic review and meta analysis

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Asian Pacific Journal of Tropical Medicine 2022年第1期15卷 17-25页

作者： Lijuan Guo Hui Xu Zhigang Yue Clinical Laboratory Emergency General HospitalNo.29 Xibahe South RoadChaoyang DistrictBeijing100028China

Objective:To investigate the resistance profiles to antimicrobial agents of wound-isolated Pseudomonas(P.)aeruginosa among Chinese burn patients.Methods:Electronic databases and manual search were used to identify eligible studies published since 2010.The objectives were pooled resistance rates for eleven common antimicrobial agents,estimated by a random-effects model.Subgroup analyses were conducted by stratifying the studies into three four-year periods based on year of isolation.Results:A total of 35 studies were included.Gentamicin had the highest pooled resistance rate(56%,95%CI 48%-64%),while meropenem had the lowest pooled resistance rate(29%,95%CI 20%-40%).There was an increasing trend of resistance to common antimicrobial agents of wound-isolated P.aeruginosa over a span of twelve years(2009-2020).There remained the highest risk of gentamicin resistance over time in China.Subgroup analyses indicated significantly higher resistances to ceftazidime and levofloxacin from 2017 to 2020.Conclusions:Enhanced resistance to common antimicrobial agents in wound-isolated P.aeruginosa presents a challenge in burn wound management in China's Mainland.Effective stewardship programs should be established based on corresponding resistance profiles,thereby optimizing treatment options for hospitalized burn patients.

关键词： antibiotic resistance Burn Nosocomial infection Pseudomonas aeruginosa

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Genetic Diversity,antibiotic resistance,and Pathogenicity of Aeromonas Species from Food Products in Shanghai,China

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Biomedical and Environmental Sciences 2022年第9期35卷 842-853页

作者： QU Feng Tian WANG Wen Qing LIU Qian ZHOU Hai Jian HU Jin Rui DU Xiao Li WANG Yue XUE Jia Qi CUI Zhi Gang XIE Gui Lin MENG Shuang College of Life Science Northeast Agricultural UniversityHa’erbin 150000HeilongjiangChina State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Shanghai Pudong New Area Center for Disease Control and Prevention Shanghai 200136China Laboratory Medicine Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Clinic Medical College North China University of Science and TechnologyTangshan 063000HebeiChina

Objective Aeromonas has recently been recognized as an emerging human pathogen.Aeromonasassociated diarrhea is a phenomenon occurring worldwide.This study was designed to determine the prevalence,genetic diversity,antibiotic resistance,and pathogenicity of Aeromonas strains isolated from food products in Shanghai.Methods Aeromonas isolates(n=79)collected from food samples were analyzed using concatenated gyrB-cpn60 sequencing.The antibiotic resistance of these isolates was determined using antimicrobial susceptibility testing.Pathogenicity was assessed usingβ-hemolytic,extracellular protease,virulence gene detection,C.elegans liquid toxicity(LT),and cytotoxicity assays.Results Eight different species were identified among the 79 isolates.The most prevalent Aeromonas species were A.veronii[62(78.5%)],A.caviae[6(7.6%)],A.dhakensis[3(3.8%)],and A.salmonicida[3(3.8%)].The Aeromonas isolates were divided into 73 sequence types(STs),of which 65 were novel.The isolates were hemolytic(45.6%)and protease-positive(81.0%).The most prevalent virulence genes were act(73.4%),fla(69.6%),aexT(36.7%),and ascV(30.4%).The results of C.elegans LT and cytotoxicity assays revealed that A.dhakensis and A.hydrophila were more virulent than A.veronii,A.caviae,and A.bivalvium.antibiotic resistance genes[tetE,blaTEM,tetA,qnrS,aac(6)-Ib,mcr-1,and mcr-3]were detected in the isolates.The multidrug-resistance rate of the Aeromonas isolates was 11.4%,and 93.7%of the Aeromonas isolates were resistant to cefazolin.Conclusion The taxonomy,antibiotic resistance,and pathogenicity of different Aeromonas species varied.The Aeromonas isolates A.dhakensis and A.hydrophila were highly pathogenic,indicating that food-derived Aeromonas isolates are potential risks for public health and food safety.The monitoring of food quality and safety will result in better prevention and treatment strategies to control diarrhea illnesses in China.

关键词： Aeromonas Genetic diversity antibiotic resistance Virulence gene Cytotoxicity assay Multidrug-resistance

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An expectation–maximization algorithm for estimating proportions of deletions among bacterial populations with application to study antibiotic resistance gene transfer in Enterococcus faecalis

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Marine Life Science & Technology 2023年第1期5卷 28-43页

作者： Yu Zhang Cong Zhang Wenwen Huo Xinlei Wang Michael Zhang Kelli Palmer Min Chen School of Mathematical Sciences Ocean University of ChinaQingdao266000China Department of Mathematical Sciences University of Texas at DallasRichardsonTX75080USA Department of Biological Sciences University of Texas at DallasRichardsonTX75080USA Department of Statistical Science Southern Methodist UniversityDallasTX75205USA MOE Key Laboratory of Bioinformatics Tsinghua UniversityBeijing100084China Department of Population and Data Sciences UT Southwestern Medical CenterDallasTX75390USA

The emergence of antibiotic resistance in bacteria limits the availability of antibiotic choices for treatment and infection control,thereby representing a major threat to human health.The de novo mutation of bacterial genomes is an essential mechanism by which bacteria acquire antibiotic resistance.Previously,deletion mutations within bacterial immune systems,ranging from dozens to thousands of base pairs(bps)in length,have been associated with the spread of antibiotic resistance.Most current methods for evaluating genomic structural variations(SVs)have concentrated on detecting them,rather than estimating the proportions of populations that carry distinct SVs.A better understanding of the distribution of mutations and subpopulations dynamics in bacterial populations is needed to appreciate antibiotic resistance evolution and movement of resistance genes through populations.Here,we propose a statistical model to estimate the proportions of genomic deletions in a mixed population based on Expectation–Maximization(EM)algorithms and next-generation sequencing(NGS)data.The method integrates both insert size and split-read mapping information to iteratively update estimated distributions.The proposed method was evaluated with three simulations that demonstrated the production of accurate estimations.The proposed method was then applied to investigate the horizontal transfers of antibiotic resistance genes in concert with changes in the CRISPR-Cas system of E.faecalis.

关键词： Bacterial genomes CRISPR-Cas system antibiotic resistance EM algorithm Proportion estimation

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Research progress on antibiotic resistance of Salmonella

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Food Quality and Safety 2022年第3期6卷 355-364页

作者： Yizhe Wang Hengwei Ge Xinyue Wei Xihong Zhao Research Center for Environmental Ecology and Engineering Key Laboratory for Green Chemical Process of Ministry of EducationHubei Key Laboratory of Novel Reactor and Green Chemical TechnologySchool of Environmental Ecology and Biological EngineeringWuhan Institut of TechnologyWuhanChina

antibiotic abuse results in various antibiotic resistance among a number of foodborne bacteria,posing a severe threat to food safety.antibiotic resistance genes are commonly detected in foodborne pathogens,which has sparked much interest in finding solutions to these issues.Various strategies against these drug-resistant pathogens have been studied,including new antibiotics and phages.Recently,a powerful tool has been introduced in the fight against drug-resistant pathogens,namely,clustered regularly interspaced short palindromic repeats-CRiSPR associated(CRISPR-Cas)system aggregated by a prokaryotic defense mechanism.This review summarizes the mechanism of antibiotic resistance in Salmonella and resistance to common antibiotics,analyzes the relationship between Salmonella CRiSPR-Cas and antibiotic resistance,discusses the changes in antibiotic resistance on the structure and function of CRiSPR-Cas,andfinally predicts the mechanism of CRISPR-Cas intervention in Salmonella antibiotic resistance.In the future,CRiSPR-Cas is expected to become an important tool to reduce the threat of antibiotic-resistant pathogens in food safety.

关键词： Foodborne pathogens antibiotic resistance CRISPR-Cas food safety

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Molecular characterization and antibiotic resistance of Salmonella enterica serovar 1,4,[5],12:i:-environmental isolates from poultry farms

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Food Quality and Safety 2022年第4期6卷 579-587页

作者： Xiaojie Qin Linlin Xiao Jiaming Li Mingzhe Yang Changying Yang Qingli Dong School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghaiChina

Salmonella enterica serovar 1,4,[5],12:i:-(S.1,4,[5],12:i:-)has been recognized as an emerging foodborne pathogen in recent years.It can cause human salmonellosis predominated by the contamination of animal-derived foods such as raw poultry and pork.This study aimed to characterize the genetic diversity,plasmid replicon types,and antibiotic resistance of 15 S.1,4,[5],12:i:-environmental isolates collected from two poultry farms using pulsed-field gel electrophoresis(PFGE),multilocus sequence typing(MLST),polymerase chain reaction-based replicon typing,and minimum inhibitory concentration approach.Ten different PFGE genotypes were detected,indicating a high diversity among these S.1,4,[5],12:i:-isolates.Three sequence types(ST19,ST1544,ST34)were identified by MLST.Among them,ST1544 was first detected in S.1,4,[5],12:i:-environmental isolates from poultry farms.All isolates were resistant to cefazolin,cefotetan,tobramycin,amikacin,and gentamicin,but susceptible to piperacillin-tazobactam,aztreonam,ceftazidime,cefepime,and ertapenem.Five incompatibility groups(Inc)of plasmids were identified,including IncFIIs(66.7%),IncHI2(20%),IncI1(6.7%),IncN(6.7%),and IncQ(6.7%).Among these isolates,80%carried at least one plasmid replicon type,and 20%carried multiple plasmid replicon types.Interestingly,the multidrug-resistant isolate 263 carried numerous resistance genes(i.***.qnrS,aac(6ʹ)-Ib-cr,bla_(TEM),bla_(CTX-M-9),bla_(OXA-1),sul1,sul2,sul3,floR,and mcr-1)and class I integronase gene intI1,which possessed both IncHI2 and IncQ plasmids,suggesting that resistance genes may be horizontally transferred by the combination of IncHI2 and IncQ plasmids.Collectively,antibiotic-resistant S.1,4,[5],12:i:-isolates were first found in poultry farm environments in China,and surveillance should be strengthened to prevent their further spread from poultry farms to foods.

关键词： Salmonella enterica serovar 1,4,,12:i:- PFGE typing MLST typing plasmid replicon typing antibiotic resistance food safety

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Roadmap to tackle antibiotic resistance in the environment under the One Health framework

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mLife 2023年第3期2卷 224-228页

作者： Liguan Li Tong Zhang Environmental Microbiome Engineering and Biotechnology Laboratory Center for Environmental Engineering ResearchDepartment of Civil EngineeringThe University of Hong KongHong KongChina

antibiotic resistance has been recognized as a major challenge worldwide for humans.“One Health”has been recognized as a key concept for containment of antibiotic resistance.Under the framework,the role of the environment in the development of antibiotic resistance genes(ARGs)has become increasingly obvious.Despite numerous efforts,response to antibiotic re-sistance is considered to be inadequate,which is probably due to the lack of a clear roadmap.Here,we propose a“One Health”roadmap to combat antibiotic resistance in the environment through(1)understanding environmental resistome.The environmental gene pool has long been recognized as the single largest reservoir of both known and novel ARGs.(2)Standardizing ARG quantification.Systematic joint efforts based on standardized quantification are urgently needed to un-derstand the true tempospatial profiles of the environmental resistome.(3)Identifying mechanisms of resistome development.Horizontal gene transfer and co-selection have been recognized as the two main mechanisms contributing to the environmental resistome.(4)Establishing a risk-assessment framework.The first critical step for large-scale cost-effective targeted ARG management in the environment is the risk assessment to identify the priority ARGs for control.(5)Formulating regulatory standards.By correlating the environmental ARG profile with public health,we may identify the indicator ARGs that can be integrated into current environmental quality standards.(6)Developing control strategies.Systematic analysis of available control technologies is required to identify the most feasible ones to curtail the spread of ARGs in the environment.The proposed roadmap under the“One Health”framework provides a guide to tackle antibiotic resistance in the environment.

关键词： antibiotic resistance environment One Health roadmap

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