检索结果-南通市图书馆

Dynamic Expressions of Liver Tissue Apoptosis-related Genes of Vibrio Vulnificus Sepsis Rats and the Effects of antibacterial agents

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2009年第2期29卷 193-197页

作者：卢中秋李萌芳梁欢邱俏檬杨光田周铁丽洪广亮 Department of Emergency Tongji Hospital Tongji Medical CollegeHuazhong University of Science and Technology Department of Emergency the First Affiliated Hospital of Wenzhou Medical College

Dynamic changes in mRNA expressions of liver tissue apoptosis-promoting genes Fas and Bax and apoptosis-inhibiting gene Bcl-2 of vibrio vulnificus sepsis rats were detected and the effects of antibacterial agents were examined. The rat model with Vibrio vulnificus sepsis （VV group） was established and some of the Vibrio vulnificus sepsis rats were treated with antibacterial agents （AA group）. The mRNA expressions of Fas, Bax and Bcl-2 were measured by reverse transcription polymerase chain reaction （RT-PCR）. As compared with normal control group （NC group）, the ex- pressions of Fas and Bax mRNA in liver tissue at all different time points in VV group were increased significantly （P〈0.05）, and the highest levels of Fas and Bax mRNA expressions were 6 and 12 h after the infection, respectively. At the same time, the expression of Bcl-2 mRNA in liver tissue at all different time points in VV group were decreased significantly （P〈0.05）, and the lowest level of Bcl-2 mRNA expression appeared 2 h after the infection. The mRNA expressions of Bcl-2 in liver tissue 9 and 12 h after the infection in AA group were increased significantly （P〈0.05） compared with NC group, while the expressions of Fas and Bax mRNA were not significantly different from those of NC group. Compared with VV group, the expression of Fas mRNA in AA group was decreased （P〈0.05） and Bax rnRNA was decreased significantly 12 and 16 h after the infection （P〈0.05）, while the expressions of Bcl-2 mRNA were increased significantly 9, 12 and 16 h after the infection （P〈0.05）. It is concluded that the mRNA expressions of liver tissue apoptosis-promoting genes Fas and Bax were increased remarkably in vibrio vulnificus sepsis rats, whereas the expression of apoptosis-inhibiting gene Bcl-2 mRNA was decreased obviously in sepsis rats in early stage. The treatment with cefoperazone sodium and levofloxacin lactate could inhibit the expression of Fas mRNA and Bax mRNA and enhance the expression ofBcl-2 rnRNA at the sam

关键词： vibrio vulnificus sepsis Fas mRNA Bax mRNA Bcl-2 rnRNA antibacterial agents

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

From Environmental Toxicants to antibacterial agents:BPA Analogues Selectively Inhibit the Growth of Gram-Positive Bacteria by Disturbing Biosynthesis of the Cell Wall

引用

Environment & Health 2023年第4期1卷 291-299页

作者： Huiming Cao Zhunjie Li Bolei Chen Jing Wang Zhen Zhou Zhi Li Yun Qian Yong Liang Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances School of Environment and HealthJianghan UniversityWuhan 430056P.R.China Department of Orthopedics General Hospital of Central Theater Command of Chinese People’s Liberation ArmyWuhan 430070P.R.China Department of Biomedical Engineering Florida International UniversityMiamiFlorida 33174United States

Remarkable antibacterial activity of BPA analogues especially for tetrabromobi-sphenol A against Staphylococcus aureus 25923(Sa25923)and methicillin-resistant Staphylococcus aureus(MRSA)has been reported in our previous ***,the toxic effects of the compounds as environmental contaminants on the endocrine system limited their applications in the field of medicine and *** the abuse of antibiotics has led to the emergence of multiple super-resistant bacteria,we considered that structural modifications based on the BPA structure will be available for molecular designing of potential antimicrobial agents without drug *** this study,to further improve the antibacterial activity and reduce the biological toxicity,we performed the computational models to evaluate the binding affinities of BPA analogues to the potential target DltA protein in the biosynthesis of cell wall.A series of synthesized achiral analogues ofα,α,α′-tris(4-hydroxyphenyl)-1-ethyl-4-isopropylbenzene(α,α,α′-TEIB)exhibited low minimum inhibitory concentration against Sa25923 and MRSA(2 or 1μg mL−1).Especially,the analogue A4 did not induce the drug-resistant mutants for all tested Gram-positive bacterial strains and exhibited relatively lower cytotoxicity in HepG2 *** developed classification model based on the light gradient boosting algorithm showed the superior performances on the internal robustness and generalization ability for the ligand-based virtual screening of bisphenol and polyphenol antimicrobial ***,our findings suggest that the molecular structure ofα,α,α′-TEIB is promising as a scaffold,which is expected to achieve a breakthrough in the development of antibiotics which can prevent the invasion of MRSA and other super bacteria.

关键词： Environmental toxicants antibacterial agents BPA analogues Lipoteichoic acid Virtual screening Machine learning

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Electrical, Thermal, Photocatalytic and antibacterial Studies of Metallic Oxide Nanocomposite Doped Polyaniline

引用

Journal of Materials Science & Technology 2013年第9期29卷 795-800页

作者： Saima Sultana Rafuddin Mohammad Zain Khan Khalid Umar M.Muneer Membrane Research Laboratory Department of Chemistry Faculty of Science Aligarh Muslim University Department of Civil Environmental and Geomatic Engineering Chadwick Building University College London Environmental Research Laboratory Department of Chemistry Faculty of Science Aligarh Muslim University

ZnO-ZrO2 nanocomposite was prepared by sol-gel method and in-situ polymerization was used to synthesize ZnO-ZrO2 nanocomposite doped polyaniline （PANI）. The materials were characterized by X-ray diffraction （XRD）, Fourier transform infrared （FTIR） spectroscopy, transmission electron microscopy （TEM）, scanning electron microscopy （SEM） and energy dispersive X-ray spectroscopy （EDX） to ensure the crystallite size, functional groups, morphology and chemical composition of the polymer nanocomposite complex. The average particle size of the ZnO-Zr02 nanoparticles was found to be 20.5 nm. Thermal gravimetric analysis （TGA） was used to study the thermal stability of the complex, shows improved thermal stability of polymer nanocomposite as compared to the pure organic polymer. The material also possesses good electrical conductivity. Additionally, the photocatalytic activity of the materials was investigated by monitoring the decolorization of Acid Blue 29 dye in a UV photocatalytic reactor and PANI-ZnO-ZrO2 was found to possess higher photocatalytic activity than pure PANI. Finally, the antibacterial activity of the materials was examined and it was observed that PANI-ZnO-ZrO2 could be used as an excellent antibacterial agent. Hence, desired properties could be integrated by mixing appropriate phases of the materials for specific applications such as heterogeneous catalyst, antibacterial agents, antibiotics delivery and high temperature conducting polymers.

关键词： Nanocomposites Polyaniline Electrical conductivity Photocatalyst antibacterial agents

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Engineered exosome hybrid copper nanoscale antibiotics facilitate simultaneous self-assembly imaging and elimination of intracellular multidrug-resistant superbugs

引用

Chinese Chemical Letters 2024年第7期35卷 319-324页

作者： Zengchao Guo Weiwei Liu Tengfei Liu Jinpeng Wang Hui Jiang Xiaohui Liu Yossi Weizmann Xuemei Wang State Key Laboratory of Digital Medical Engineering School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Department of Chemistry Ben-Gurion University of the NegevBeer-Sheva 8410501Israel

With the increasing emergence of bacterial infections,especially multidrug-resistant(MDR)bacteria,poses an urgent *** study demonstrated a novel multifunctional nanotheranostics platform developed by the strategic integration of both in-situ bio-assembly imaging and target bacteria *** the introduction of copper ions into bacteria,the Cu^(2+)could spontaneously bio-selfassembled into a multifunctional copper nanoclusters(NCs)which efficiently enhanced epigallocatechin gallate(EGCG)uptake into *** visualizing the bacteria,the developed theranostic nanoplatform exhibited highly efficient disinfection activities with negligible side effects as reflected by higher cell viability and insignificant hemolytic ***,the exosomal formulation of EGCG integrated with Cu^(2+)showed an increased intracellular antibacterial activity,which could eliminate most of the methicillin-resistant Staphylococcus aureus(MRSA)phagocytosed by macrophages,guide macrophages toward M2-like phenotype polarization and alleviate inflammation,without exhibiting obvious cytotoxicity on host *** regimen could be viewed as an effective strategy for the sterilization of intractable bacterial infections.

关键词： In situ self-assembled Exosome antibacterial agents Bioimaging Intracellular infection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comparative efficacy and optimal duration of first-line antibiotic regimens for acute otitis media in children and adolescents:a systematic review and network meta-analysis of 89 randomized clinicaltrials

引用

World Journal of Pediatrics 2024年第3期20卷 219-229页

作者： Min Seo Kim Jae Han Kim Seohyun Ryu Seung Won Lee Dong Keon Yon Eunyoung Kim Ai Koyanagi Elena Dragioti Jae Il Shin Lee Smith Samsung Advanced Institute forHealth Sciences&Technology(SAIHST) Samsung Medical CenterSungkyunkwan UniversitySeoulRepublic of Korea Yonsei University Collegeof Medicine SeoulRepublicof Korea Department of Precision Medicine Sungkyunkwan University School of MedicineSuwonRepublic of Korea Center for Digital Health Medical Science Research InstituteKyung Hee University College of MedicineSeoulRepublic of Korea Evidence-Based Research Laboratory Department of Clinical Pharmacy and Pharmaceutical CareCollege of PharmacyChung-Ang UniversitySeoulRepublic of Korea Parc Sanitari Sant Joan de Deu/CIBERSAM/ISCIII Universitat de BarcelonaFundacio Sant Joan de Déu.Sant Boi de LlobregatBarcelonaSpain ICREA Pg.Lluis Companys 23BarcelonaSpain Pain and Rehabilitation Centre and Department of Medical and Health SciencesLinkoping University58185 LinkopingSweden Department of Pediatrics Yonsei University College of MedicineYonsei-ro 50Seodaemun-gu8044Seoul 03722Republic of Korea Centre for Health Performanceand WellbeingAnglia Ruskin UniversityCambridge CB11PTUK Research Laboratory Psychology of Patients Families&Health ProfessionalsDepartment of NursingSchool of Health SciencesUniversity of IoanninaIoannina 45500Greece

Introduction Antibiotic use for acute otitis media(AOM)is one of the major sources of antimicrobial ***,the effective minimal antibiotic duration for AOM remains ***,guidelines often recommend broad ranges(5-10 days)of antibiotic use,yet the clinical impact of such a wide window has not been *** We systematically searched PubMed/MEDLINE,Embase,Scopus,Web of Science,and Cochrane Library from database inception to 6 October *** meta-analysis was conducted on randomized controlled trials that assessed antibiotic treatment for AOM in children(PROSPERO CRD42020196107).Results For amoxicillin and amoxicillin-clavulanate,7-day regimens were noninferior to 10-day regimens in clinical responses[amoxicillin:risk ratio(RR)0.919(95%CI 0.820-1.031),amoxicillin-clavulanate:RR 1.108(0.957-1.282)],except for≤2 *** the third-generation cephalosporins,7-day and 10-day regimens had similar clinical responses compared to placebo[7-day:RR 1.420(1.190-1.694),10-day:RR 1.238(1.125-1.362)compared to placebo].However,5-day regimens of amoxicillin-clavulanate and third-generation cephalosporins were inferior to 10-day *** to amoxicillin,a shorter treatment duration was tolerable with *** Our findings indicated that 1O days of antibiotic use may be unnecessarily long,while the treatment duration should be longer than 5 ***,5-day regimens would be sufficient for a modest treatment *** findings revealed that the current wide range of recommended antibiotic durations may have influenced the clinical outcome of AOM,and a narrower antibiotic duration window should be re-established.

关键词： Amoxicillin-potassium Amoxicillin antibacterial agents Cephalosporins Duration of therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of Multiple Cleaning and Disinfection Interventions on Infectious Diseases in Children: A Group Randomized Trial in China

引用

Biomedical and Environmental Sciences 2015年第11期28卷 779-787页

作者： BAN Hai Qun LI Tao SHEN Jin LI Jin PENG Pin Zhang YE Heng Ping ZHANG Liu Bo Institute of Environment Health and Related Product Safety Center for Disease Control and Prevention of China Center for Disease Control and Prevention of Xiantao city

Objective To assess the effectiveness of multiple cleaning and disinfection interventions in the homes and kindergartens, in reducing gastrointestinal and respiratory illnesses of children. Methods From October 2010 to September 2011, we performed a prospective, controlled study in China. 408 children under 5 years old were recruited and group randomized into intervention and control groups. Families and kindergartens in the intervention group were provided with antibacterial products for hand hygiene and surface cleaning or disinfection for one year. Each child＇s illness symptoms and sick leave were recorded every day. Results A total of 393 children completed the study, with similar baseline demographics in each of the 2 groups. Except for abdominal pain, the odds of symptoms （fever, cough and expectoration, runny nose and nasal congestion, diarrhea）, illness （acute respiratory illness and gastrointestinal illness）, and sick leave per person each month were significantly reduced by interventions. The rates of fever, diarrhea, acute respiratory illness, gastrointestinal illness and sick leave per person per year were significantly decreased as well. Conclusion Not only the acute respiratory children were significantly reduced by multiple and gastrointestinal illness but the sick leave rate in interventions.

关键词： Communicable disease control Child daycare centers Sick leave antibacterial agents Hand hygiene Domestic hygiene Surface cleaning Surface disinfection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Anti-hemolytic, antibacterial and anti-cancer activities of methanolic extracts from leaves and stems of Polygonum odoratum

引用

Asian Pacific Journal of Tropical Biomedicine 2018年第12期8卷 580-585页

作者： Nittaya Chansiw Narisara Paradee Kamonnaree Chotinantakul Somdet Srichairattanakool School of Medicine Mae Fah Luang University Department of Biochemistry Faculty of MedicineChiang Mai University

Objective: To investigate anti-hemolytic, antibacterial and anti-cancer activities of leaf and stem extracts from Polygonum odoratum. Methods: Leaves and stems of Polygonum odoratum were extracted using methanol and their anti-hemolytic activity was assessed using 2, 2′-Azobis(2-methylpropionamidine) dihydrochloride which is known to generate free radical damage on cell membranes of red blood cells. This damage, represented by hemolysis, was measured using spectrophotometry. antibacterial activity was tested by using a broth microdilution method to find minimal inhibitory concentrations against eight bacterial strains. Anti-cancer activity of the extracts was evaluated against a human promyelocytic leukemic cell line(HL-60) by using MTT assay for cell viability and flow cytometry for apoptosis induction and cell cycle analysis. Results: Both leaf and stem extracts have anti-hemolytic activity. The results showed a significantly increased percentage of inhibition in a concentration-dependent manner. Interestingly, the leaf extract showed anti-hemolytic activity to a greater extent than the stem extract. antibacterial activity of the extracts, as indicated by their minimal inhibitory concentration, using 12.5, 50, 25, 25 μg/mL, was measured against Staphylococcus epidermidis, Enterococcus faecium, Enterococcus faecalis and Staphylococcus aureus. The leaf extracts also exhibited anti-cancer activity, demonstrated by significantly decreased cell viability of human promyelocytic cells(HL-60), with an IC_(50) of(350.00±1.85) μg/mL for 48 h and(38.00±0.92) μg/mL for 72 h. Additionally, HL-60 became apoptotic and accumulated in G_1-phase after 48 hours of treatment. Conclusions: The extracts of Polygonum odoratum exhibit potential antihemolytic activity. They also have antibacterial activity by inhibiting growth of Gram-positive bacteria. The leaf extract shows anti-cancer activity against HL-60 to a greater extent than the stem extract, causing decreased viability, increas

关键词： Polygonum odoratum Anti-hemolysis antibacterial agents Anti-cancer agents

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preliminary pharmacological evaluation of Alocasia indica Schott tuber

引用

Journal of Integrative Medicine 2013年第5期11卷 343-351页

作者： Md.Khirul Islam Imran Mahmud Sanjib Saha Asit Baron Sarker Himangsu Mondal A.S.M.Monjur-Al-Hossain Md.Anisuzzman Phytochemistry and Pharmacology Research Laboratory Pharmacy Discipline Life Science SchoolKhulna University

To elucidate potential antioxidant, antidiarrheal, cytotoxic, and antibacterial activities of the ethanol extract of A/ocasia indica Schott tuber in different experimental models established in vitro and in vivo. METHODS： In vitro antioxidant activity was evaluated by 2, 2-diphenyl-l-picrylhydrazyl （DPPH） radical-scavenging assay. Phenolic content was estimated by using Folin-Ciocalteu＇s reagent while reducing ability was measured by ferric reducing power assay./n vivo antidiarrheal studies were carried out in mice, and the activity was evaluated in castor oil and magnesium sulfate- induced diarrhea. Disk diffusion assay was utilized to determine antibacterial activity against a number of pathogenic bacterial strains. Acute toxicity test was carried out to measure the safe doses for the extract. RESULTS： In DPPH radical-scavenging assay, the extract exhibited strong radical-scavenging activity with the 50% inhibitory concentration value of 42.66 IJg/mL. Total phenolic content was found to be 542.26 mg gallic acid equivalent per 100 g of dried tuber extract, whereas flavonoid content was found to be 4.30 mg quercetin equivalent/g of dried tuber extract. In reducing power assay, the extract showed strong reducing power in a concentration-dependent manner. The extract significantly （P 〈 0.01） enhanced the latent period and decreased defecation in both castor oil- and magnesium sulfate-induced diarrhea. The extract also lessened gastrointestinal motility in mice. Potential antibacterial activity was exhibited by the extract against all the tested bacterial strains in disk diffusion assay. The 50% lethal concentration against brine shrimp nauplii was 81.09 μg/mL. CONCLUSION： The results demonstrated that the ethanol extract of A. inc/ica has potential antioxidant, antidiarrheal, cytotoxic, and antibacterial activity.

关键词： Alocasia plant extracts antioxidants antidiarrheals antibacterial agents cytotoxins mice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Review of copper and copper alloys as immune and antibacterial element

引用

Transactions of Nonferrous Metals Society of China 2022年第10期32卷 3163-3181页

作者： Yang-gang WANG Hao-yang LI Xiao-yan YUAN Yan-bin JIANG Zi-an XIAO Zhou LI School of Materials Science and Engineering Central South UniversityChangsha 410083China Teaching and Research Section of Clinical Nursing Xiangya HospitalCentral South UniversityChangsha 410008China Department of Otolaryngology Second Xiangya HospitalCentral South UniversityChangsha 410011China

This review discussed the relationship among copper,human,and *** plays an important role in human *** can boost human immune defense reactions at recommended intake *** content mainly focused on copper antibacterial activity and copper antibacterial *** stated that copper antibacterial activity is affected by copper homeostasis mechanisms in bacteria,adhesion,humidity,strain specificity,and manufacturing methods of antibacterial *** the preparation of particle antibacterial agents and surface antibacterial agents,this review discussed several manufacturing methods,such as sol−gel,cold spray,and biosynthesis belonging to chemical synthesis,physical synthesis,and biological synthesis,***−gel method contributes to the preparation of particle agents and surface *** spray technique is utilized in synthesis of surface copper *** is a novel technology which can be applied in nanoparticle agent preparation.

关键词： immunity toxicity antibacterial activity copper homeostasis adhesion humidity strain specificity antibacterial agents mechanisms

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Spinal Epidural Abscess in Patients with Sepsis Who Experienced Significant Improvements after Complete Paralysis: Two Case Reports

引用

Open Journal of Emergency Medicine 2020年第4期8卷 125-133页

作者： Tomofumi Ogoshi Motoo Yoshimiya Hiroshi Ichibakase Takayoshi Kimura Masafumi Kameoka Hayato Yoshioka Takahiro Ueda Masato Homma Department of Emergency and Critical Medicine Tottori University Hospital Yonago Japan

Spinal epidural abscess (SEA) is a rare condition, and a delay in its diagnosis causes paralysis. In this study, we report two rare cases of delayed diagnosis of SEA whose conditions improved after a state of complete paralysis. The first case was a 71-year-old diabetic man who received a corticosteroid injection for shoulder pain that caused intensified pain. Thereafter, the patient developed paralysis of both legs in stage IV according to Heusner staging. Subsequently, he was diagnosed with multiple abscesses and sepsis. He was in a poor state of health. Therefore, we treated his epidural abscess conservatively. After a month, his muscle strength had improved to Heusner stage III-A, and he was transferred to another hospital. The second case was a 64-year-old diabetic man who received an epidural corticosteroid injection for lower back pain. However, the pain intensified and was admitted to the hospital for pyelonephritis. He developed paralysis in both of his arms and legs presenting as a Heusner stage IV, caused by a cervical epidural abscess. A laminoplasty was performed and paralysis was improved. However, the patient subsequently developed a left subcortical hemorrhage. He underwent surgery. However, his right hemiplegia persisted, and on Day 21, he was transferred back to the previous hospital with a Heusner III-A. We could improve the patients’ paralysis by cooperating closely with infectious disease specialists and spine surgeons, taking intensive care, applying antibacterial agents appropriately, and operating quickly.

关键词： antibacterial agents Diabetes Mellitus Iatrogenic Abscesses Infection Spinal Epidural Abscess

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：