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Purification,characterization and antiproliferative activity of L-asparaginase from Aspergillus oryzae CCT 3940 with no glutaminase activity

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Asian Pacific Journal of Tropical Biomedicine 2016年第9期6卷 785-794页

作者： Fernanda Furlan Goncalves Dias Ana Lucia Tasca Gois Ruiz Adriana Della Torre Helia Harumi Sato Department of Food Science School of Food EngineeringUniversity of Campinas Division of Pharmacology and Toxicology Multidisciplinary Center for ChemicalBiological and Agricultural ResearchUniversity of Campinas

Objective:To explore the anti-proliferative activity of purified L-asparaginase from Aspergillus oryzae CCT 3940(***).Methods:L-asparaginase was produced by submerged fermentation and purified to electrophoresis homogeneity by ionic exchanged chromatography in a fast protein liquid chromatographic *** purified enzyme was characterized and used for the antiproliferative assay against nine tumor cell lines and one non-tumor cell ***:The free glutaminase L-asparaginase was purified 28.6 fold.L-asparaginase showed high stability under physiological condition,remaining stable in the p H range7.0–8.0 after 1 h incubation at temperature range 30–45℃.The Km and Vmax values of purified L-asparaginase were estimated as 0.66 mmol/L and 313 IU/mL,*** purified enzyme could inhibit the growth of a broad range of human tumor cell lines at the concentrations ***,the enzyme from *** CCT 3940 could inhibit tumor growth of leukemia cell line(K562) with a total growth inhibition value of(3.2 ± 2.5) IU/mL and did not inhibit the non-carcinogenic human cell line growth at the concentrations ***:The sensitivity of the cells lines to purified L-asparaginase from *** CCT 3940 appeared to be concentration dependent affording a more significant decrease in cell growth than that observed for the commercial L-asparaginase from Escherichia *** L-asparaginase from *** CCT 3940 has a high potential for pharmaceutical exploitation in the treatment of leukemia.

关键词： L-asparaginase Aspergillus oryzae Purification anti-tumoral activity

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Polyoxygenated Cembranoids from Soft Coral Lobophytum Crassum and Their anti-tumoral Activities

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Chinese Journal of Chemistry 2021年第3期39卷 640-646页

作者： Fang-Zhou Yin Xia-Juan Huan Wayan Mudianta Ze-Hong Miao Hong Wang Yue-Wei Guo Xu-Wen Li State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of Sciences555 Zu Chong Zhi RoadZhangjiang Hi-Tech ParkShanghai 201203China Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals and College of Pharmaceutical Science Zhejiang University of TechnologyHangzhouZhejiang 310014China Department of Chemistry Universitas Pendidikan GaneshaBali 81116Indonesia

Main observation and conclusion Four new cembranoids,6-oxo-cembrene-A(1),lobocrassins G-H(2-3),and 14-epi-lobophytolide B(4),along with eight known re-lated compounds(5-12),were isolated from the South China Sea soft coral Lobophytum *** structures were established by extensive spectroscopic analysis and by comparison of the spectral data with those reported in the *** absolute stere-ochemistry of 14-epi-lobophytolide B(4)was established by time-dependent density functional theory electronic circular dichroism(TDDFT-ECD)calculations,whereas that of three known metabolites(6,11 and 12)was also determined,for the first time,by using modified Mosher's method and/or X-ray diffraction *** bioassay,the cembranoids 6,7 and 12 showed significant an-ti-tumoral activity against A549,HT-29,SNU-398 and Capan-1 tumor cell lines with IC_(50) values ranging from 1.5 to 7.4μmol/L.A pre-liminary structure-activity relationship was also discussed.

关键词： Soft coral Lobophytum crassum Cembranoids anti-tumoral activity Stereochemistry

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