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Quercetin exerts anti-inflammatory effects via inhibiting tumor necrosis factor-α-induced matrix metalloproteinase-9 expression in normal human gastric epithelial cells

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World Journal of Gastroenterology 2022年第11期28卷 1139-1158页

作者： Hsi-Lung Hsieh Ming-Chin Yu Li-Ching Cheng Mei-Yi Chu Tzu-Hao Huang Ta-Sen Yeh Ming-Ming Tsai Department of Nursing Division of Basic Medical SciencesChang-Gung University of Science and TechnologyTaoyuan 333TaiwanChina Research Center for Chinese Herbal Medicine College of Human EcologyChang Gung University of Science and TechnologyTaoyuan 333TaiwanChina Department of Neurology Chang Gung Memorial HospitalTaoyuan 333TaiwanChina Department of General Surgery New Taipei Municipal TuCheng HospitalNew Taipei 236TaiwanChina College of Medicine Chang Gung UniversityTaoyuan 333TaiwanChina Department of General Surgery Chang Gung Memorial HospitalTaoyuan 333TaiwanChina

BACKGROUND Gastric injury is the most common digestive system disease worldwide and involves inflammation,which can lead to gastric ulcer or gastric cancer(GC).Matrix metallopeptidase-9[MMP-9(gelatinase-B)]plays an important role in inflammation and GC progression.Quercetin and quercetin-rich diets represent potential food supplements and a source of medications for treating gastric injury given their anti-inflammatory activities.However,the effects and mechanisms of action of quercetin on human chronic gastritis and whether quercetin can relieve symptoms remain unclear.AIM To assess whether tumor necrosis factor-α(TNF-α)-induced MMP-9 expression mediates the anti-inflammatory effects of quercetin in normal human gastric mucosal epithelial cells.METHODS The normal human gastric mucosa epithelial cell line GES-1 was used to establish a normal human gastric epithelial cell model of TNF-α-induced MMP-9 protein overexpression to evaluate the antiinflammatory effects of quercetin.The cell counting Kit-8 assay was used to evaluate the effects of varying quercetin doses on cell viability in the normal GES-1 cell line.Cell migration was measured using Transwell assay.The expression of proto-oncogene tyrosine-protein kinase Src(cSrc),phospho(p)-c-Src,extracellular-signal-regulated kinase 2(ERK2),p-ERK1/2,c-Fos,p-c-Fos,nuclear factor kappa B(NF-κB/p65),and p-p65 and the effects of their inhibitors were examined using Western blot analysis and measurement of luciferase activity.p65 expression was detected by immunofluorescence.MMP-9 m RNA and protein levels were measured by quantitative reverse transcription polymerase chain reaction(q RT–PCR)and gelatin zymography,respectively.RESULTS q RT-PCR and gelatin zymography showed that TNF-αinduced MMP-9 m RNA and protein expression in a dose-and time-dependent manner.These effects were reduced by the pretreatment of GES-1 cells with quercetin or a TNF-αantagonist(TNFR inhibitor)in a dose-and timedependent manner.Quercetin and TNF-αantagonists decreased the TNF-α-induced phosphory

关键词： anti-inflammatory Quercetin Matrix metallopeptidase-9 Tumor necrosis factor-α Normal human gastric epithelial cells

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Enhanced bioavailability and biosafety of cannabidiol nanomicelles for effective anti-inflammatory therapy

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Particuology 2022年第10期20卷 1-9页

作者： Yuan Rao Runwei Li Saixing Liu Linchao Meng Qilin Wu Qipeng Yuan Hao Liang Meng Qin College of Life Science and Technology Beijing University of Chemical TechnologyBeijing 100029China StarHealth Botanical Technology Corporation Beijing 100005China

Cannabidiol(CBD)shows great anti-inflammatory potential;however,the hydrophobicity and strong first-pass effect of CBD leads to its extremely low oral bioavailability.Poloxamer 407(P407)is a tri-block copolymer composed of(poly)ethylene oxide(PEO)and(poly)propylene oxide(PPO)sections.It has a PEO-PPO-PEO structure,which is widely used in the preparation of drug delivery systems that are highly biocompatible.When it reaches a certain concentration in water,P407 can self-assemble into a micelle structure containing a hydrophobic core and a hydrophilic shell.A potential approach to enhanc-ing the oral bioavailability of hydrophobic drugs incorporating them into the hydrophilic carrier.We prepared CBD nanomicelles with a drug loading of 14.29%by a cosolvent evaporation method using P407 with appropriate antioxidants.Cell experiments indicated that anti-inflammatory markers(IL-4 and IL-10)increased,while inflammatory markers(TNF-αand IL-6)decreased.Moreover,animal experiments showed that inflammatory cells were inhibited by CBD nanomicelles,and the anti-inflammatory effect of micelles was better than that of CBD,while no obvious evidence indicated toxicity to the liver and kidney.

关键词： CBD Poloxamer 407 Polymeric micelle anti-inflammatory Cytotoxicity

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Improvement of antibacterial, anti-inflammatory, and osteogenic properties of OGP loaded Co-MOF coating on titanium implants for advanced osseointegration

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Journal of Materials Science & Technology 2023年第15期146卷 131-144页

作者： Bailong Tao Weiwei Yi Xian Qin Junjie Wu Kai Li Ai Guo Jie Hao Lixue Chen Laboratory Research Center The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Rehabilitation Medicine The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Obstetrics and Gynecology Chongqing Health Center for Women and ChildrenChongqing 401147China Women and Children’s Hospital of Chongqing Medical University Chongqing 401147China Department of Orthopedics The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Orthopedics The Third Affiliated Hospital of Chongqing Medical UniversityChongqing 400016Chin

The bacterial infection,especially for methicillin-resistant Staphylococcus aureus(MRSA),and the associated severe inflammatory response could extremely limit the crosstalk of RAW264.7 cells and mesenchymal stem cells(MSCs)and lead to the undesirable osseointegration of peri–implants.It is highly demanded to modify the surface of titanium(Ti)implant to improve its anti-bacterial and anti-inflammatory properties and facilitate its disabled osseointegration.Herein,in our study,a multifunctional coating of zeolitic imidazolate frameworks-67 encapsulated osteogenic growth peptide(OGP)(ZO)was fabricated on titanium dioxide nanotubes(TNT)substrates(TNT-ZO)via the electrophoresis deposition(EPD)approach.The TNT-ZO substrates exhibited excellent antibacterial activity indicated by the reactive oxygen species(ROS)generation,outer membrane(OM)and inner membrane(IM)permeabilization change,adenosine triphosphate(ATP)decrease,and intracellular compounds(DNA/RNA)leakage.Importantly,the regulation effects of TNT-ZO coating modified titanium substrates on the RAW264.7-MSCs crosstalk could induce the anti-inflammatory and osteogenic microenvironment via multiple paracrine signaling of Runx2,BMP2,VEGF,and TGF-β1.The promoted effects of coating structure were investigated in vivo,including antibacterial effect,osteogenic differentiation of mesenchymal stem cells,and anti-inflammation of RAW264.7 cells,as well as infected bone regeneration and repair in bone defect transplantation model.The results demonstrated that MRSA was effectively eliminated by the hydrolysis of ZIF-67 nanoparticles on TNT-ZO substrates.Furthermore,the excellent osseointegration of peri–implants was realized simultaneously by modulating the crosstalk of RAW264.7-MSCs.This study could provide a novel approach to designing a multifunctional coating on the Ti implants for infected bone regeneration in orthopedic applications.

关键词： Titanium implants Osteogenic growth peptide anti-inflammatory antibacterial activity Osteoimmunomodulation

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Continuously released Zn^(2+)in 3D-printed PLGA/β-TCP/Zn scaffolds for bone defect repair by improving osteoinductive and anti-inflammatory properties

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Bioactive Materials 2023年第6期24卷 361-375页

作者： Chunxu Li Fengbo Sun Jingjing Tian Jiahao Li Haidan Sun Yong Zhang Shigong Guo Yuanhua Lin Xiaodan Sun Yu Zhao Department of Orthopedics Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China State Key Laboratory of Advanced Ceramics and Fine Processing School of MaterialsTsinghua UniversityBeijingChina Medical Science Research Center Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Core Facility of Instrument Institute of Basic Medical SciencesChinese Academy of Medical Sciences and School of Basic MedicinePeking Union Medical CollegeBeijingChina The State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and School of Basic MedicinePeking Union Medical CollegeBeijingChina Department of Rehabilitation Medicine Southmead HospitalBristolUK

Long-term nonunion of bone defects has always been a major problem in orthopedic treatment.Artificial bone graft materials such as Poly(lactic-co-glycolic acid)/β-tricalcium phosphate(PLGA/β-TCP)scaffolds are expected to solve this problem due to their suitable degradation rate and good osteoconductivity.However,insufficient mechanical properties,lack of osteoinductivity and infections after implanted limit its large-scale clinical application.Hence,we proposed a novel bone repair bioscaffold by adding zinc submicron particles to PLGA/β-TCP using low temperature rapid prototyping 3D printing technology.We first screened the scaffolds with 1 wt%Zn that had good biocompatibility and could stably release a safe dose of zinc ions within 16 weeks to ensure long-term non-toxicity.As designed,the scaffold had a multi-level porous structure of biomimetic cancellous bone,and the Young’s modulus(63.41±1.89 MPa)and compressive strength(2.887±0.025 MPa)of the scaffold were close to those of cancellous bone.In addition,after a series of in vitro and in vivo experiments,the scaffolds proved to have no adverse effects on the viability of BMSCs and promoted their adhesion and osteogenic differentiation,as well as exhibiting higher osteogenic and anti-inflammatory properties than PLGA/β-TCP scaffold without zinc particles.We also found that this osteogenic and anti-inflammatory effect might be related to Wnt/β-catenin,P38 MAPK and NFkB pathways.This study lay a foundation for the follow-up study of bone regeneration mechanism of Zn-containing biomaterials.We envision that this scaffold may become a new strategy for clinical treatment of bone defects.

关键词： 3D printing Zinc submicron particles Osteoinductivity anti-inflammatory Bone defect repair

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Purifi cation and identification of anti-inflammatory peptides from sturgeon (Acipenser schrenckii) cartilage

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Food Science and Human Wellness 2023年第6期12卷 2175-2183页

作者： Li Yuan Qian Chu Bei Yang Wei Zhang Quancai Sun Ruichang Gao School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013China Bio-resources Key Laboratory of Shaanxi Province School of Biological Science and EngineeringShaanxi University of TechnologyHanzhong 723001China

Cartilage is a nonedible byproduct with little saleable value.However,previous studies have proposed the possibility of producing peptides from cartilage with immune function modulation potential.The current study aimed to investigate the potential anti-inflammatory activity of peptides derived from sturgeon(Acipenser schrenckii)cartilage in lipopolysaccharide(LPS)-stimulated RAW264.7 macrophages.Five peptide sequences,including four novel peptides,were identified from ethanol-soluble cartilage hydrolysates.Among these five peptides,LTGP,LLLE,LLEL and VGPAGPAGP reduced the production of nitric oxide(NO)and interleukin-6(IL-6)while increasing interleukin-10(IL-10)excretion.Transcriptome analysis suggested the inhibition of activated mitogen-activated protein kinase(MAPK)and interleukin-17(IL-17)signaling pathways after LLEL intervention.MAPK,which is involved in the IL-17 signaling pathway,was further proved to be blocked by downregulating the phosphorylation of p38,extracellular-signal regulated protein kinase(ERK),and c-jun N-terminal kinase(JNK).This novel peptide offers an attractive approach to develop functional foods.

关键词： Sturgeon cartilage Peptides anti-inflammatory Mitogen-activated protein kinase

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Selenium-enriched oolong tea(Camellia sinensis)extract exerts anti-inflammatory potential via targeting NF-κB and MAPK pathways in macrophages

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Food Science and Human Wellness 2022年第3期11卷 635-642页

作者： Qi Wang Juqing Huang Yafeng Zheng Xuefang Guan Chenchun Lai Huiying Gao Chi-Tang Ho Bin Lin Institute of Agricultural Engineering Fujian Academy of Agricultural SciencesFuzhou 350003China College of Food Science Fujian Agriculture and Forestry UniversityFuzhou 350002China Fujian Key Laboratory of Agricultural Product(Food)Processing Fuzhou 350003China Department of Food Science Rutgers UniversityNew BrunswickNJ 08901USA

Both tea polyphenols and selenium(Se)have been suggested to exert the health benefits via the regulatory capacities of chronic inflammation,which make Se-enriched oolong tea a promising beverage as an anti-inflammatory diet.The aim of this study is to investigate the anti-inflammatory effects of Se-enriched oolong tea extract(Se-TE)and underlying mechanism in lipopolysaccharide(LPS)-induced RAW264.7 cells.Se-TE treatments(50 and 150μg/m L)significantly suppressed the over-production of nitric oxide(NO)and prostaglandin E2(PGE2)in LPS-stimulated macrophages via downregulating the expression of nitric oxide synthase(i NOS)and cyclooxygenase-2(COX-2).Moreover,Se-TEs also effectively inhibited the productions of inflammatory cytokines,such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and interleukin-1β(IL-1β).Furthermore,Se-TE could block mitogen-activated protein kinase(MAPK)and nuclear factor-kappa B(NF-κB)signaling pathways through the inhibition of the phosphorylation of key proteins(IκB-α,p65,p38,ERK,and JNK)and the translocation of the p65 subunit into the nucleus.Collectively,our results indicated that Se-TE may have the potential to be used as a novel food ingredient for the development of various anti-inflammatory foods and the treatment and prevention of chronic inflammation-related diseases.

关键词： Oolong tea Selenium anti-inflammatory NF-κB and MAPK pathways

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Cytotoxic Properties on Cervical and Liver Cancer Cells of Two Plant Recipes from Burkina Faso

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Journal of Biosciences and Medicines 2024年第6期12卷 216-229页

作者： Estelle Ouédraogo Bagora Bayala Théodora Mahoukèdè Zohoncon Samiratou Ouédraogo Olga Mélanie Lompo Essi Etonam Dovo Jean-Marie Compaoré Albert Théophane Yonli Jacques Simpore Laboratory of Molecular Biology and Genetics (LABIOGENE) Joseph KI-ZERBO University Ouagadougou Burkina Faso Center of Biomolecular Research Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Ecole Normale Suprieure Koudougou Burkina Faso Hspital Saint Camille of Ouagadougou (HOSCO) Ouagadougou Burkina Faso Faculty of Health University Saint Thomas dAquin (USTA) Ouagadougou Burkina Faso Chair Research and Action against Cancer Department of Public Health Training and Research Unit in Health Sciences Joseph KI-ZERBO University Ouagadougou Burkina Faso National Population Health Observatory National Institute of Public Health Ouagadougou Burkina Faso The Department of Epidemiology Biostatistics and Occupational Health School of Population and Global Health Montral Canada Laboratory of Pathological Anatomy and Cytology University Joseph KI-ZERBO Ouagadougou Burkina Faso TIIG LA TIIM Ouagadougou Burkina Faso

Cancer is one of the deadliest diseases in developing countries. In recent years, natural plant-based compounds have been used in the search for drugs to combat numerous diseases, including cancer. In this study, we evaluate the cytotoxic properties of paanfo tiben 1 and paanfo tiben 2, two traditional herbal formulations from Burkina Faso used in the treatment of cancer in Burkina Faso. To this end, the recipes were infused and freeze-dried. The dry extracts obtained were used to determine total phenolics and flavonoids content, assess antioxidant activity using the DPPH, ABTS and FRAP methods, evaluate anti-inflammatory properties by inhibiting 15-LOX, COX 1 and 2, and assess cytotoxic activity on HeLa cervical cancer and HePG2 liver cancer cell lines using the MTT test. The paanfo tiben 1 recipe showed the highest levels of total phenolics and flavonoids, as well as the best antioxidant activities, with IC50 values of 21.020 ± 0.6 µg/ml and 22.94 ± 0.57 µg/ml for DPPH and ABTS, and 165.15 mM EAA/mg dry extract for FRAP. It also exhibited the best cytotoxic activity with IC50 values of 112.02 ± 0.025 µg/ml on HeLa cells and 80.67 ± 6.08 µg/ml on HepG2 cells. On the other hand, paanfo tiben 2 exhibited the best anti-inflammatory activities through inhibition of 15-LOX and COX 1, with inhibition percentages at 100 µg/ml of 32.523% and 24.717 % respectively. These results could justify the traditional use of these two recipes by traditional health practitioners in the treatment of cancer sufferers in Burkina Faso.

关键词： antioxidant anti-inflammatory Cytotoxicity Paanfo Tiben Burkina

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Optimization of LPS-Induced Inflammation Model and Its Feasibility as a Fast Screening Model for Cosmetics

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Journal of Cosmetics, Dermatological Sciences and Applications 2024年第1期14卷 84-97页

作者： Fanghui Sun Xiaojie Song Nannan Liu Gang Huo R & D Center OSM Biology Co. Ltd. Huzhou China

Objectives: The existing inflammatory models are concentrated in relatively complex medical fields, and most of them use a single type of cell, and the induction conditions are not uniform, so the current LPS-induced inflammation model is less conducive to the study of skin inflammation. The aim of this research is to enhance the existing LPS-induced inflammation model and establish a skin inflammation model that is suitable for the swift screening of anti-inflammatory agents in the cosmetics industry. Methods: LPS was used to induce inflammatory responses in KC and THP-1 cells. Enzyme-linked immunosorbent assay (ELISA) was employed to assess the levels of IL-1α, IL-8, and TNF-α in the two cell types, while the DCFH-DA probe was utilized to label the levels of reactive oxygen species (ROS) in both cell types. Results: In KC cells, 10 μg/mL of LPS induced a significant upregulation of IL-8 but did not result in elevated expression of IL-1α. However, at 100 μg/mL of LPS, both IL-8 and IL-1α were highly expressed in KC cells. LPS concentrations ranging from 0.01 to 100 μg/mL failed to stimulate TNF-α production in KC cells but induced a gradient increase in ROS levels. In THP-1 cells, LPS concentrations from 0.01 to 100 μg/mL did not induce IL-1α production but significantly elevated IL-8 and led to a gradient increase in TNF-α and ROS. After treatment with 100 μg/mL of LPS, the cosmetic ingredient Rucika KGM mitigated the elevated levels of IL-1α, IL-8, and ROS in LPS-induced KC cells and IL-8 and ROS in THP-1 cells. Conclusion: This study has successfully developed an application-oriented model suitable for investigating skin inflammation, enabling the rapid and comprehensive screening of cosmetic ingredients with anti-inflammatory activity. .

关键词： Cell Culture anti-inflammatory Lipopolysaccharide Keratinocytes THP-1 inflammatory Factors

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anti-Tumor and anti-Diabetic Effects of Sarsasapogenin

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Medicinal Plant 2024年第1期15卷 59-61页

作者： Mingxinzhi WANG Jingchao WANG Quan QUAN Xichun HUANG Xinyu LIU Chenghao JIN College of Life Science and Biotechnology Heilongjiang Bayi Agricultural UniversityDaqing 163319China

In this paper,the pharmacological effects and molecular mechanisms of sarsasapogenin,such as anti-oxidant,anti-inflammatory and anti-diabetic effects,are reviewed in order to provide a theoretical basis for the subseq... 详细信息

关键词： Sarsasapogenin anti-tumor anti-diabetic anti-inflammatory

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Effect of Elephantopus scaber L.Extract on Acute Pleurisy in Rats

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Medicinal Plant 2024年第1期15卷 48-50,56页

作者： Yan ZHANG Jianting GAO Xinchao SHI Hailong LIU Xin HE Institute of Animal Husbandry and Veterinary Medicine Hainan Academy of Agricultural SciencesHaikou 571100China College of Veterinary Medicine Hebei Agricultural UniversityBaoding 071000China

[Objectives]To study the anti-inflammatory effect of Elephantopus scaber L.extract on acute pleurisy induced by carrageenan in rats,and to explore its anti-inflammatory mechanism.[Methods]The active sites of E.scaber L.were extracted by ethanol reflux method.The extracts of different concentrations of E.scaber L.were used as the study object,and dexamethasone was used as the positive control drug.The anti-inflammatory effects of E.scaber L.extracts were studied by measuring the levels of malondialdehyde(MDA),prostaglandin E_(2)(PGE_(2)),tumor necrosis factor(TNF-α),interleukin-1β(IL-1β)in pleural fluid and serum nitric oxide(NO),MDA,PEG_(2),TNF-α,IL-1βin rats with acute pleurisy induced by carrageenan.[Results]E.scaber L.extracts in three doses could reduce the levels of inflammatory factors in pleural fluid and serum,and inhibit acute pleurisy in rats.It was speculated that the anti-inflammatory mechanism was related to the inhibition of the release of inflammatory factors and the antioxidant effect of extracts of three doses of E.scaber L.[Conclusions]This experiment provides a basis for the development and application of E.scaber L.

关键词： Elephantopus scaber L. Extract Acute pleurisy anti-inflammatory

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