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Peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via MAPK and NF-κB pathways

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Food Science and Human Wellness 2021年第1期10卷 103-111页

作者： Ruichang Gao Wanghui Shu Yang Shen Quancai Sun Wengang Jin Dajing Li Ying Li Li Yuan School of Food and Biological Engineering Jiangsu UniversityZhenjiangJiangsu Province212013China Bio-resources Key Laboratory of Shaanxi Province School of Bioscience and EngineeringShaanxi University of TechnologyHanzhong723001China Institute of Agro-product Processing Jiangsu Academy of Agricultural SciencesNanjing210014JiangsuChina

Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of sturgeon peptides and their underlying mechanisms are unknown. The current study was therefore to determine the anti-inflammatory potential of sturgeon peptides with lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model. Pepsin hydrolysate (PeH) was purified by ultrafiltration and Sephadex G-15 gel filtration chromatography. PeH significantly reduced the inflammatory mediator (NO) and inflammatory cytokines (IL-6, TNF-α and IL-1β) expression in a dose-dependent manner. Moreover, the purified sturgeon peptide (F2) possessed strong antioxidant potential and effectively inhibited DPPH and ABTS free radicals. F2 significantly suppressed the expression of MAPK, IκBα, and NF-κB p65, indicating that F2 exerted anti-inflammatory influence by the inhibition of MAPK and NF-κB pathways.

关键词： Sturgeon Enzymatic hydrolysis antioxidant anti-inflammation mechanism RAW264.7 macrophages

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Effects of Dandelion Extract on the Proliferation of Rat Skeletal Muscle Cells and the Inhibition of a Lipopolysaccharide-lnduced Inflammatory Reaction

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Chinese Medical Journal 2018年第14期131卷 1724-1731页

作者： Qiang Liu Heng Zhao Yue Gao Yan Meng Xiang-Xuan Zhao Shi-Nong Pan Department of Radiology Shengjing Hospital China Medical University Shenyang Liaoning 110004 China Department of Radiology The First Affiliated Hospital of University of South China Hengyang Hunan 421001 China

Background： Dandelion is commonly used in traditional Chinese medicine with several active compounds found in extracts. It has a variety of pharmacological effects, such as a reduction in swelling and inflammation, and detoxification. The mechanism by which dandelion extract inhibits the inflammatory response in skeletal muscle cells remains unknown; therefore, the aim of this study was to investigate the effects of dandelion extract root on the proliferation of skeletal muscle cells and the alleviation of lipopolysaccharide （LPS）-induced inflammatory response in vitro. Methods： Rat skeletal muscle cells were isolated from Sprague-Dawley rat and cultured in vitro which were cultured in basal medium, or medium containing LPS or dandelion extract. Cell counting kit-8 （CCK-8） was employed to measure cell proliferation; meanwhile, the optimal concentration of dandelion extract and treatment time were selected. Crystal violet staining was used to detect the proliferation of muscle cells. Western blotting analysis was used to detect the levels of inflammatory factors, myogenic factor, and p-AKT protein expression. Results： The optimal concentration and treatment time of dandelion extract for the following study were 5 mg/ml and 4 days, respectively. Dandelion extract was found to increase proliferation of rat skeletal muscle cells （t = 3.145, P 〈 0.05）, with the highest effect observed at 5 mg/ml. LPS was found to decrease proliferation of skeletal muscle cells （t = -131.959, P 〈 0.001）, and dandelion extract could against this affection （t = 19.466, P 〈 0.01）. LPS could induce expression of inflammatory factors, including interleukin （IL）-16, IL-6 and tumor necrosis factor （TNF）-α （IL-16： t = 9.118, P 〈 0.01; IL-6： t = 4.346, P 〈 0.05; TNF-α： t = 15.806, P 〈 0.05）, and dandelion extract was shown to reduce LPS-induced expression of IL- 16, IL-6 and TNF-α （IL-I 6： t = -2.823, P 〈 0.05; IL-6： t = -3.348, P 〈 0.01; and TNF-α： t = -3.710, P 〈 0.01）. Furthermore, LPS was also

关键词： anti-inflammation mechanism Dandelion Extract Herbal Plant inflammation Skeletal Muscle Cells

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