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3D Printed Gelatin Scaffold with Improved Shape Fidelity and Cytocompatibility by Using antheraea pernyi silk fibroin nanofibers

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Advanced Fiber Materials 2022年第4期4卷 758-773页

作者： Shengzhi Zou Suna Fan Ana L.Oliveira Xiang Yao Yaopeng Zhang Huili Shao State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Shanghai Engineering Research Center of Nano‑Biomaterials and Regenerative MedicineCollege of Materials Science and EngineeringDonghua UniversityShanghai 201620People’s Republic of China CBQF‑Centro de Biotecnologia e Quimica Fina‑Laboratorio Associado Escola Superior de BiotecnologiaUniversidade Catolica PortuguesaRua Diogo Botelho 13274169‑005 PortoPortugal

Gelatin(G)is a commonly used natural biomaterial owing to its good biocompatibility and easy ***,using pure gelatin as a bioink can barely achieve an ideal shape fidelity in 3D *** this study,antheraea pernyi silk fibroin nanofibers(ASFNFs)with arginine-glycine-aspartic acid(RGD)peptide and partial natural silk structure are extracted and combined with pure gelatin bioink to simultaneously improve the shape fidelity and cytocompatibility of corresponding 3D printed *** show that the optimum printing temperature is 30℃ for these *** printed filaments using 16G/4ASFNFs bioink(16wt%gelatin and 4wt%ASFNFs)demonstrate better morphology and larger pore size than those printed by pure gelatin bioink(20G,20wt%gelatin),thus successfully improve the shape fidelity and porosity of the 3D printed *** 16G/4ASFNFs scaffold also demonstrate higher swelling ratio and faster degradation rate than the pure gelatin ***,the cell viability and proliferation ability of Schwann cells cultured on the 16G/4ASFNFs scaffold are significantly superior than those cultured on the pure 20G *** ASFNFs enhanced 16G/4ASFNFs scaffold reported here are expected to be a candidate with excellent potential for biomedical applications.

关键词： antheraea pernyi silk fibroin nanofiber 3D printed scaffold Shape fidelity Cytocompatibility Gelatin

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