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G418-Resistance as a Dominant Selectable Marker for Heterogenous Gene Expression in Antagonist Pichia membranefaciens

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Agricultural Sciences in China 2005年第1期4卷 41-46页

作者： WANYa-kun TIANShi-ping KeyLaboratoryofPhotosynthesisandEnvironmentalMolecularPhysiology InstituteofBotanyTheChineseAcademyofSciencesBeijing100093P.R.China

Pichia membranefaciens, which was isolated from the surface of peach fruits, showed effective biocontrol capabilityagainst rhizopus rot of peach fruits. Aminoglycoside antibiotic G418 can inhibit the growth of P. membranefaciens. Theminimal inhibitory concentration of G418 to P. membranefaciens in YPD medium was 100g mL-1. We constructed aphosphoglycerate kinase (PGK) promoter-driven neoR expression cassette, which was called pFL61-neo. The biocontrolyeast P. membranefaciens was transformed with pFL61-neo by lithium acetate method. Expression vector pFL61-neoconferred P. membranefaciens drug resistance to 100g mL-1 G418. The transformant could keep a high percentage ofplasmid-containing of transformant with 67.87% after 50 generations in non-selective medium. The result showed that *** could recognize the promoter and terminator of PGK and direct the expression of heterologous neoRgene. Expression vector pFL61-neo could exist stably in P. membranefaciens. Therefore, it is feasible to utilize G418-resistance as a dominant selectable marker for heterogenous gene expression in antagonist P. membranefaciens.

关键词： antagonistic yeast Biocontrol G418-resistance Dominant selectable marker

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Optimization of Media for Production of an Effective yeast Biocontrol Agent Pichia membranefaciens

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Agricultural Sciences in China 2004年第4期3卷 286-291页

作者： WANYa-kun TIANShi-ping KeyLaboratoryofPhotosynthesisandEnvironmentalMolecularPhysiology InstituteofBotanyChineseAcademyofSciencesBeijing100093P.R.China

The growth of Pichia membranefaciens was studied using different nitrogen and carbonsources as substrates. Among nitrogen sources tested, soya peptone, yeast extract power,beef extract and polypeptone were relatively favorable to the growth of yeast. Thedensity of the yeast showed to be directly proportional to carbon sources *** and fructose were good carbon sources for the yeast growth. However, lactoseshowed poor performance for the cell growth of the yeast. In this study, beef extractpresented a good synergic effect on the yeast growth with different carbonhydrates. Themedium for *** used glucose and beef extract as substrates. The higherconcentration of glucose and beef extract, the better growth of ***. Theaddition of chlorella growth factor (CGF) stimulated markedly the growth of *** increased concentration of CGF from 0.5 to 1% did not enhance the numbers ***. This result will help design a better strategy for scale-up produc-tion of ***.

关键词： Pichia membranefaciens antagonistic yeast Media Optimization

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Effect of β-glucan on stress tolerances and biocontrol efficacy of Cryptococcus laurentii against Penicillium expansum in pear fruit

Effect of β-glucan on stress tolerances and biocontrol effi...

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中国植物病理学会2015年学术年会

作者： FU Da ZENG Li-zhen ZHENG Xiao-dong YU Ting College of Biosystems Engineering and Food Science Fuli Institute of Food ScienceZhejiang Key Laboratory for Agro-Food ProcessingZhejiang R＆D Center for Food Technology and EquipmentZhejiang University

The objective of this study was to determine the effect of β-glucan on stress tolerances and biocontrol efficacy of Cryptococcus laurentii against Penicillium expansum in pear *** results showed that biocontrol efficacy and the population growth of *** harvested from nutrient yeast dextrose broth amended with p-glucan at 0.5%（NYGB） were significantly higher compared to that harvested from nutrient yeast dextrose broth（NYDB）.Moreover,the viability of *** under heat,oxidative and water stress was markedly improved by cultivating in *** addition,the activity of superoxide dismutase in yeast cells was induced after exposure to heat,oxidative and water *** results indicate that the application of(3-glucan might be an effective method to improve the stress tolerances and biocontrol efficacy of *** our knowledge,this is the first report regarding the function of(3-glucan in enhancing various stress tolerances of post-harvest biocontrol yeasts.

关键词： antagonistic yeast Blue mold Postharvest disease Stress tolerance

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