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Oil Crop Science 2021年第1期6卷 1-7页

作者： Jing Zhang Yu Wang Zhaolin Du Dasong Lin Lili Huo Li Qin Wei Wang Liwen Qiang Yanpo Yao Yi An Innovative Team of Monitoring and Precaution for Cropland Environment Agro-environmental Protection InstituteMinistry of Agriculture and Rural AffairsTianjinChina

Aflatoxin is a strong carcinogenic and toxic fungal toxin produced by Aspergillus flavus and other Aspergillus species,and can seriously threaten the health of consumers,thus becoming a global concern.Corn,as an important oil and economic crop,is highly susceptible contaminated by aflatoxin.In this study,antagonistic bacteria with strong inhibitory effect on aflatoxin were screened to provide support for the treatment aflatoxin contamination control in corn.Ten strains which have strong antagonistic effects against A.flavus were isolated from healthy corn from different corn producing areas in China.Among them,the antagonistic bacteria JTFM1001 through corn kernels in vivo and field experiment,the inhibition effect of aflatoxin contamination reached above 70%and 55%,respectively.And the strain was identified as Bacillus subtilis based on its morphological,physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA.In addition,our data showed that it can colonize in the rhizosphere and survive for a long time,forming the dominant flora,with broad application prospect.Finally,we were surprised to find that the antibacterial metabolites secreted by the antagonistic bacteria was one of the mechanisms of its inhibition of A.flavus and aflatoxin.This will provide us with new ideas and perspectives on the effective prevention and control of aflatoxin contamination in corn and corn oil.

关键词： Aflatoxin contamination Aspergillus flavus Corn Corn oil antagonistic bacteria antagonistic effect

antagonistic Effects of Sphingomonas and Pseudomonas aeruginosa on 4 Kinds of Pathogenic bacteria of Ginseng

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Asian Agricultural Research 2022年第5期14卷 31-35页

作者： Hairu YU Feifan YAN Yunlong WANG Xinying TONG Di CHEN Qiang YE Renzhe PIAO Hongyan ZHAO Yanbian Characteristic Industry Development Center Yanji 133002 China College of Agriculture Yanbian University Yanji 133002 China Longjing Agricultural Technology Extension Station Longjing 133400 China Yanbian Korean Autonomous Prefecture Academy of Agricultural Sciences Longjing 133400 China

[Objectives]To explore effective biocontrol methods for diseases in the process of ginseng cultivation,and develop an efficient and environmentally friendly biocontrol agent.[Methods]In this study,2 strains were isolated from biogas slurry,and Cylindrocarpon destructans(XF),Fusarium solani(GF),Botrytis cinerea Pers(HM)and Alternaria panax Whetz(HB)were used as test materials.The strains were isolated and identified by dilution plate method,16S rDNA sequence identification method,confrontation culture method,filter paper method and ultraviolet spectrophotometer method,and the bacteriostatic activity and bacteriostatic rate were tested.[Results]Strain 15(Sphingomonas)and strain 19(Pseudomonas aeruginosa)were screened out through identification and analysis,and they grew stably within 8-10 d.The bacteriostatic rates of strain 15 against A.panax and B.cinerea were 47.37%and 43.40%,respectively,and the bacteriostatic rates of strain 19 against A.panax and B.cinerea were 62.30%and 63.27%,respectively.The bacteriostatic activity of the extract of strain 19 increased with the increase of OD_(600) value,and the bacteriostatic effect was optimal when the OD_(600) value was in the range of 0.8-1.0,up to 70%,so it had a strong biocontrol potential.[Conclusions]This experiment provides convenience for more effective inoculation,establishes a fast,simple and accurate method for the determination of the best bacteriostatic rate of P.aeruginosa culture solution to HM,and lays a foundation for large-scale culture of P.aeruginosa culture solution.Besides,it is expected to provide a theoretical basis for the efficient control of ginseng B.cinerea in field production,use it for the prevention and control of ginseng shoot diseases,and provide a reference for the efficient and diverse development of biocontrol agents for ginseng shoot diseases.

关键词： Panax ginseng C.A.Meyer Ginseng diseases antagonistic bacteria Screening Identification

Isolation of antagonistic bacteria against Fusarium graminearum and Its antagonistic Activity

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Plant Diseases and Pests 2016年第5期7卷 13-15,20页

作者： Sun Cai Zhang Peng Huang Shi Li Hongyan Ma Dongfang Zhang Changqing Hubei Collaborative Innovation Center for Grain Industry/Yangtze University Jingzhou 434025 China

[ Objective] To develop biological control resources of endophytic bacteria in wheat, the bacteria with antagonistic effect against Fusarium graminearum were screened and their antagonistic activity was studied. [ Method] The endophytic bacteria of wheat with antagonistic effect were screened using plate confrontation method; the antagonistic bacteria were identified through physiological and biochemical characteristics and 16S rDNA sequence analysis; the antagonistic spectrum of antagonistic bacteria was studied using plate confrontation method. [ Result] The antagonistic bacterium XY-3 was isolated from wheat leaves, and was identified as Bacillus amyloliquefaciens according to the physiological and biochemical characteristics and 16S rDNA sequence analysis. [ Conclusion] XY-3 had antagonistic effect against four kinds of common pathogenic fungi, presenting broad spectrum antagonistic activity against plant pathogenic fungi.

关键词： Fusarium head blight（FHB） antagonistic bacteria Endophytic bacteria Bacillus amyloliquefaciens

Potential of Some antagonistic bacteria for Biological Control of Potato Late Blight Caused by Phytophthora infestans

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Potential of Some Antagonistic Bacteria for Biological Contr...

中国菌物学会2018年学术年会

作者： Long Han Jichuan Kang Bangxing Lei College of Life Science Guizhou University Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China Guizhou University

Seven bacteria strains（Bacillus amyloliquefaciens D1, Stenotrophomonas maltophilia he41, Bacillus amyloliquefaciens Hd213, Bacillus subtilis H3, Bacillus amyloliquefaciens DEB-2, Brevundimonas bullata Hd13,Lysinibacillus fusiformis he14） were tested in vitro for antagonistic properties against Phytophthora infestans, the causal agent of potato late blight. Different antagonistic bacteria were selected according to their inhibition effect on growth of Phytophthora infestans that caused potato late blight via dual-culture method. The inhibition rates of the 7 strains against mycelia growth of Phytophthora infestans were tested via flat plate pair method, of which 4 strains viz. he14, Hd213, Hd13 and D1 had the inhibition effect greater than 60%, and he14 had the highest inhibition effect of 67%. Meanwhile the fermentation broth of the strains showed significant inhibition effect on Phytophthora infestans, and the effect was enhanced by increasing the concentrations of the both. When their broth concentrations were 4%, the inhibition rates of D1 and H3 strains reached 100% respectively. In addition,the inhibition rates of all strains were 100% with the broth conentrations of 64%, which indicated that these bacterial strains had a great potential for the biological control of potato late blight.

关键词： Potato late blight,Phytophthora infestans antagonistic bacteria Biological control

Screening,Identification and Inhibition effect of antagonistic bacteria against Fusarium oxysporum f.sp.nevium

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Screening,Identification and Inhibition effect of Antagonist...

中国植物病理学会2017年学术年会

作者：李萍 Liu Dong 高智谋 Bi Zhangyou Department of Horticulture and Landscape Anqing Vocational and Technical College School of Plant Protection Anhui Agricultural University

An antagonistic bacterial strain BTF5 against Fusarium oxysporum f.***.niveum was isolated from rhizosphere soil collected of continual watermelon mono-cropping by dual-culture,and identified as Burkholderia vietnamiensis according to morphology,biochemical assay and homology analysis of 16 S rDNA sequence.Inhibition rate of the strain BTF5 against F.oxysporum f.***.niveum was up to 63.09%in the antagonistic experiment.Less change in the inhibition effect of antagonizing against growth of F.oxysporum f.***.niveum was observed during 8 generations of transfers on artificial media,indicating its potential biocontrol efficacy and application prospect.

关键词： Fusarium oxysporum f sp niveum antagonistic bacteria Screening

Study on Biological characteristic of Leaf Blight Pathogen and antagonistic bacteria from Camellia oleifera

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Study on Biological characteristic of Leaf Blight Pathogen a...

第三届中国森林保护学术大会

作者： Li Lin~1 Zhou Guo-ying~1 Li He~(1,2) 1 Central South University of Forestry&Technology,College of Forestry,Changsha,410004,China. 2 Central South University of Forestry&Technology,Biotechnology Core Facilities,Changsha,410004,China.

Leaf blight of Camellia oleifera is one of the major diseases in Camellia producing areas of China.The pathogen infected leaves of the host plants,resulting in earlier defoliation,new treetops death, and yield reduction.The pathogen of leaf blight(NO.CSUFT070160B) was isolated from the infected leaves of Camellia oleifera.In order to provide the basis for control Leaf blight of Camellia oleifera,the rDNA ITS sequences and the characteristic of the pathogen had been studied,and the antagonistic bacteria was screened.The results showed that the ITS region of the rDNA from the pathogen was sequenced 516bp. By comparing them with the sequences in the Gene Bank,we found that the strain was highest homologous to Pestalotiopsis microspora;and the concordance ratio is 99%.The molecule phylogenetic tree indicated that the CSUFT070160B and DQ000995 were in the same branch with high confidence level.Combined with morphological characteristics,the strain was checked as P.microspora.Then we studied the characteristic of the pathogen,the results showed that the pH tolerance at a range of pH4 to pH9,and the fastest mycelium growth was at pH 6;the temperature tolerance at a range of 5℃to 31℃,and 27℃was the most appropriate temperature;the light has little effect on mycelium growth.The medium which takes sucrose as carbon source was the best for the pathogen mycelium growth,takes glucose as carbon source would caused the slowest growth.For the control of the Camellia oleifera leaf blight,antagonistic bacteria was screened from 175 endophytic bacterias by confront culture method.The results showed that the antagonistic effect of YB18 strain was the best with 84.7%inhibitory rate.According to the results of the morphology,cultural characteristics,physiological and biochemical propertices,16S gene sequence and molecular phylogenetic tree,YB18 was identified as the strain belonging to Bacillus subtilis.The results will lay a foundation for preventing and control the Camellia oleifera leaf blight.

关键词： Camellia oleifera leaf blight Pathogen antagonistic bacteria biological control

Isolation and Identification of antagonistic bacteria Against Soil-borne Pathogenic Fungus of Tobacco

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Isolation and Identification of Antagonistic Bacteria Agains...

中国植物病理学会2016年学术年会

作者：宋喜乐 DING Yue-qi 康业斌 College of Forestry Henan University of Science and Technology

This study is aimed to isolate and screen bacteria strains from tobacco rhizospheric soil,which have antagonistic activities against soil-borne pathogenic fungus of tobacco.Soil dilution plating technique and dual culture technique on agar plate were used for isolate and screen antagonistic bacteria.The antagonistic strains were identified by morphological features observation,physiological and biochemical experiments,and 16 S rDNA sequence analysis.30 rhizospheric soil were collected from tobacco filed in Luoning,Ruyang,Songxian and Yiyang countries in Luoyang,Henan province.180 bacterial isolates were obtained from 30 rhizosphere soil samples by separation and purification,and 49 strains have antagonistic function to the soil-born pathogenic fungi tested in this trial.Strains TS681、TS682、TS683were strongly antagonistic to three kinds of pathogenic fungi,and the inhibition zones to Pythium aphanidermatum,Phytophthora parasitica and Furasium oxysporum respectively more than 7.0 mm,10.0 mm and 9.0mm with the antagonistic rate respectively reaching above 51.4%,68.8%and 60.0%.All this three strains were identified as Paenibacillus polymyxa,and they may have the potential value of development and application for biological controlling of soil-borne fungi diseases of tobacco.

关键词： Tobacco Soil-born pathogenic fungi antagonistic bacteria Paenibacillus polymyxa

Gram-Positive bacteria Associated with Rice in China and Their Antagonists Against the Pathogens of Sheath Blight and Bakanae Disease in Rice

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Rice science 2005年第3期12卷 213-218页

作者： Luo Jin-yan XIE Guan-lin LI Bin Luo Yuan-chan ZHAO Li-han WANG Xiao LIU Bo LI Wen State Key Laboratory of Rice Biology Zhejiang University Hangzhou 310029 China Center ofBiotechnology Fujian Academy of Agricultural Science Fuzhou 350003 China

It is necessary to understand the bacterial populations associated with rice so as to provide more information and natural resources for effective management of major diseases in rice. A survey on screening and identification of gram-positive bacteria was conducted during 1998 2004. Seven hundred and fifty-six rice samples were collected from Zhejiang, Jiangsu, Fujian and Yunnan Provinces, China. Over 1000 bacterial isolates were isolated and tested for colony morphology, pathogenicity, and some characteristics of bacteriology including Gram staining, fluorescent pigment on Kings medium B and microscopic observation for endospore. Together with five standard reference strains, 74 representative gram-positive bacterial isolates were confirmed by Biolog and gas chromatographic analysis of fatty acid methyl esters. Five bacterial species of Bacillus and other three genera were identified and isolates from Bacillus sublitis and Bacillus megaterium, exhibited the most effective inhibition against the pathogens of sheath blight and bakanae disease of rice. A few isolates from Bacillus pumilus and Bacillus megaterium showed weak virulent on rice together with some virulent isolates, risk should be considered when isolates from these species were screened for biocontrol agents.

关键词： bacterial identification bacterial population antagonistic bacteria sheath blight bakanae disease rice

Fire Blight Disease Caused by Erwinia amylovora on Rosaceae Plants in Kyrgyzstan and Biological Agents to Control This Disease

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Advances in Microbiology 2016年第11期6卷 831-851页

作者： Tinatin Doolotkeldieva Saykal Bobusheva Plant Protection Department Faculty of Agriculture Kyrgyz-Turkish Manas University Bishkek Kyrgyzstan

Erwinia amylovora species were isolated from the blossoms, exudates, infected fruits, leaves and bent branches of diseased apple, pear and hawthorn trees, selected in the Chy, Osh and Jalal Abad regions. Biochemical and pathogenicity tests, alongside PCR analyses, were conducted to identify the local isolates of Erwinia amylovora. The alternative antagonistic microorganisms which combat bacterium E. amylovora were tested within in vitro and in vivo conditions. The results revealed the ability of Streptomyces antagonistic bacteria to decrease fire blight severity on pear and apple trees during the first stage of the fire blight disease in leaf tissues. Streptomyces strain C1-4 suppressed E. amylovora disease symptoms in the leaf tissues and excised apple and pear shoots. The incidence of fire blight on leaves was reduced by about 70% with two applications of bacterial antagonists. Further studies at different locations in Kyrgyzstan, using large scale application, would allow for stronger recommendations to be made, including studies and recommendations on their ability to prevent disease and to use them as main components in an integrated pest management program.

关键词： Biological Control Agents Fire Blight Erwinia amylovora antagonistic bacteria

Studies on Possible Modes of Action and Tolerance to Environmental Stress Conditions of Different Biocontrol Agents of Foliar Diseases in Maize

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Agricultural Sciences 2020年第6期11卷 552-566页

作者： Melina Sartori Martín Bonacci Paula Barra Aluminé Fessia Miriam Etcheverry Andrea Nesci Germán Barros Laboratorio de Ecología Microbiana Ambiental (ECOMA) Departamento de Microbiología e Inmunología Facultad de Ciencias Exactas Universidad Nacional de Río Cuarto Córdoba Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Buenos Aires Argentina

The present study evaluates the possible modes of action of antagonistic bacteria and their tolerance to UV radiation, temperature and osmotic stress. The partial 16S-23S rRNA gene sequencing of eight antagonistic bacteria had a high match with three bacterial genera: Curtobacterium, Pantoea and Bacillus. In this study, the three Bacillus isolates showed the most relevant production of enzymes, volatile organic compounds and antibiosis against Exserohilum turcicum. Respect to UV radiation and temperature Pantoea and Bacillus isolates were more tolerant;whereas the eight isolates were tolerant to osmotic stress in varying degree. The three Bacillus isolates have the greatest potential as biocontrol agents for foliar diseases in maize. The antagonistic action could be explained through different modes of action such as enzymes, volatile organic compounds and/or direct antibiosis by other secondary metabolites. Bacillus isolates tolerance to environmental stresses including UV radiation, temperature and osmotic stress is relevant for survival and persistence on the leaf surface. This work provides new information about the mode of action of antagonistic bacteria with proven efficacy against maize leaf pathogens. In addition, it provides information about the tolerance of antagonistic bacteria against different stress conditions. The data of the present study could contribute to the development of a successful foliar biofungicide.

关键词： Biocontrol Maize antagonistic bacteria Bacillus Exserohilum turcicum