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Insect Science 2010年第4期17卷 335-343页

作者： Vipin Madhulika Dube S. K. Gakhar Advanced Centre for Biotechnology Department of Statistics Maharshi Dayanand University Rohtak India

anopheles stephensi is the main vector of urban malaria in South Asia. Three ecological variants （‘type’, ‘mysorensis’ and ‘intermediate’） of An. stephensi have been reported on the basis of ecology and egg morphology. However, it is unclear if there is any genetic isolation between the three variants. We analyzed the three variants of An. stephensi using eight microsatellite loci and found that large and significant genetic differentiation exists between them （mean FST = 0.393 and mean RST = 0.422）. Pairwise estimates of genetic differentiation between the variants were ‘type’ versus ‘mysorensis’ （mean FsT = 0.411 and mean RST = 0.308）, ‘type’ versus‘intermediate’ （mean FsT = 0.388 and mean RST = 0.518） and ‘intermediate’versus ＇mysorensis＇（mean FST = 0.387 and mean RST = 0.398） and all were statistically significant （P 〈 0.05）. The greater sensitivity of RST in differentiation indicated that mutations and not genetic drift had generated the differences between three variants of An. stephensi. The present study indicated large genetic differentiation and presence of non-significant low level ofgene flow between the three variants （‘type’,‘ mysorensis’ and ‘intermediate’） of An. stephensi.

关键词： anopheles stephensi ecological variants gene flow intermediate mysorensis type

The impact of climatic variables on the population dynamics of the main malaria vector, anopheles stephensi Liston(Diptera: Culicidae), in southern Iran

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Asian Pacific Journal of Tropical Medicine 2020年第10期13卷 448-455页

作者： Madineh Abbasi Abbas Rahimi Foroushani Tohid Jafari-Koshki Kamran Pakdad Hassan Vatandoost Ahmad Ali Hanafi-Bojd Deparment of Medical Entomology&Vector Control School of Public HealthTehran University of Medical SciencesTehranIran Infectious and Tropical Diseases Research Center Tabriz University of Medical SciencesTabrizIran Department of Epidemiology and Biostatistics School of Public HealthTehran University of Medical SciencesTehranIran Molecular Medicine Research Center Tabriz University of Medical SciencesTabrizIran Department of Statistics and Epidemiology Faculty of HealthTabriz University of Medical SciencesTabrizIran Department of Parasitology&Mycology Paramedical SchoolShahid Beheshti University of Medical SciencesTehranIran

Objective:To determine the significance of temperature,rainfall and humidity in the seasonal abundance of anopheles stephensi in southern ***:Data on the monthly abundance of anopheles stephensi larvae and adults were gathered from earlier studies conducted between 2002 and 2019 in malaria prone areas of southeastern *** data for the studied counties were obtained from climatology *** estimating equations method was used for cluster correlation of data for each study site in different ***:A significant relationship was found between monthly density of adult and larvae of anopheles stephensi and precipitation,max temperature and mean temperature,both with simple and multiple generalized estimating equations analysis(Panopheles stephensi and max temperature was significant(P<0.05).Conclusions:This study provides a basis for developing multivariate time series models,which can be used to develop improved appropriate epidemic prediction systems for these ***-term entomological study in the studied sites by expert teams is recommended to compare the abundance of malaria vectors in the different areas and their association with climatic variables.

关键词： anopheles stephensi Climatic variables Monthly activity Iran

Wash resistance and bio-efficacy of Olyset~? Plus, a long-lasting insecticide-treated mosquito net with synergist against malaria vector, anopheles stephensi

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Asian Pacific Journal of Tropical Medicine 2017年第9期10卷 887-891页

作者： Soraya Sheikhi Hassan Vatandoost Mohammad Reza Abai Mansoreh Shayeghi Ahmad Raeisi Morteza Akbari Fatemeh Nikpoor Mohammad Sistanizade Aghdam Akbar Bagheri Department of Medical Entomology&Vector Control School of Public HealthTehran University of Medical SciencesTehranIran Department of Environmental Chemical Pollutants and Pesticides Institute for Environmental ResearchTehran University of Medical SciencesTehranIran Department of Malaria CDCMinistry of Health&Medical EducationIran

Objective: To determine the wash resistance of Olyset~? Plus using World Health Organization Pesticide Evaluation Scheme standard washing procedure and to assess the value of knock down and mortality rates of anopheles stephensi at different regimens of long lasting insecticide treated nets ***: The study was conducted at the Bioassay Laboratory of Culicidae Insectary,School of Public Health, Tehran University of Medical Sciences, Iran. The net was made of polyester impregnated with permethrin and piperonyl butoxide at a ratio of 2:1. The washing resistance was assessed using Le Chat?soap and a shaker incubator set at a speed of 155 r/min, 30℃ for 10 min. The cone bioassay test was carried out according to World Health Organization recommended guideline with tolerant field strain of female anopheles stephensi to ***: The knockdown and mortality rates of female mosquitoes exposed to Olyset~? Plus from un-washed nets to 2 washings were 79.7% and 88.8% respectively. Mortality was dropped to zero while active ingredient estimated 0.532 mg/100 cm^2 to 0.481 mg/100 cm^2 after 15 washings. A positive correlation was seen between residues of permethrin on nets, knockdown rate and mortality rate of female anopheles stephensi exposed to different regimes of washed Olyset~? Plus(r = 0.954, P = 0.001).Conclusions: It is recommended that a preliminary survey conducted on resistance level of anopheles vectors before the distribution of Olyset~? Plus in malaria endemic communities.

关键词： Long-lasting nets Permethrin Wash resistance Residue anopheles stephensi

Mosquito larvicidal properties of volatile oil from salt marsh mangrove plant of Sesuvium portulacastrum against anopheles stephensi and Aedes aegypti

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Journal of Coastal Life Medicine 2013年第1期1卷 66-71页

作者： Mohamed Yacoob Syed Ali Venkatraman Anuradha SyedAbudhair Sirajudeen Prathasarathy Vijaya Nagarajan Yogananth Ramachandran Rajan Peer Mohamed Kalitha Parveen PG&Research Department of Biotechnology Mohamed Sathak College of Arts&ScienceSholinganallurChennai-600119Tamil NaduIndia Center for Marine Fisheries Research Institute Mandapam CampTamil NaduIndia

Objective:To identify the larvicidal activity of the volatile oil from Sesuvium portulacastrum(***)against anopheles stephensi and Aedes ***:Volatile oil extract of *** was prepared in a graded series of *** test for the larvicidal effect of volatile oil against mosquitos larvae was conducted in accordance with the WHO standard *** of 25 early 4th instar larvae of two mosquitoes were transferred to 250 mL enamel bowl containing 199 mL of distilled water and 1 mL of plant *** experiment was conducted with triplicate with concurrent a control ***:Volatile oil extract of *** showed toxicity against 4th instar larvae of Aedes aegypti and anopheles stephensi with equivalent LC50 value[(63±7.8)μL/mL,LCL-UCL=55.2-64.0]and LC90 value[(94.2±3.9)μL/mL]in maximum activity with minimum concentration(200μL/mL)of volatile oil and followed by maximum activity of 250μL concentration showed LC50 value=(68.0±8.2)μL/mL,LCL-UCL=66.26-69.2 and LC50 value of(55.2±2.8)μL/mL,LCL-UCL=53.7-56.9,LC90=(95.2±1.25)μL/mL and followed by 250μL of oil extract against 4th instar larvae of Aedes aegypti ***:It is inferred from the present study that,the extracts from salt marsh mangrove plan of *** are identified as a potential source of safe and efficacious mosquito control agents for the management of vector borne diseases of malaria and dengue.

关键词： Aedes aegypti anopheles stephensi Larvicidal Oleic acid Sesuvium portulacastrum

Mosquitocidal activity of indigenenous plants of Western Ghats,Achras sapota Linn.(Sapotaceae)and Cassia auriculata L.(Fabaceae)against a common malarial vector,anopheles stephensi Liston(Culicidae:Diptera)

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Journal of Coastal Life Medicine 2014年第5期 402-410页

作者： Kaliyamoorthy Krishnappa Kuppusamy Elumalai Department of Advanced Zoology and Biotechnology Govt.Arts College(Autonomous)NandanamChennai–600035TamilnaduIndia

Objective:To evaluate the mosquito larvicidal,ovicidal,pupicidal and repellent activities of hexane,diethyl ether,dichloromethane,acetone and methanol extracts of Indian medicinal plants,Achras sapota(***)and Cassia auriculata(***)at different concentrations against anopheles stephensi(***),a malarial ***:Twenty five early third instar larvae of *** were exposed to various concentrations(30-210 mg/L)of plants extracts and were assayed in the laboratory by using the protocol of WHO 2005;then after 24 h LC_(50) values of the *** and *** leaf extract was determined by probit *** ovicidal activity was tested with the extracts ranging from 50-350 mg/*** pupicidal activity was recorded after 24 h of exposure to the *** repellent efficacy was determined against mosquito species at two different concentrations 1.5 and 3.0 mg/cm^(2) under laboratory ***:Among the five different extracts tested against the ***,methanol extract of *** proved to be an more effective solvent extract in almost all the parameters studied than ***:It is inferred that the leaf extract of *** and *** could be used in vector control programme.

关键词： Achras sapota Cassia auriculata anopheles stephensi Larvicidal activity Ovicidal activity Pupicidal activity Repellent activity

Mosquito larvicidal properties of Impatiens balsamina(Balsaminaceae)against anopheles stephensi,Aedes aegypti and Culex quinquefasciatus(Diptera:Culicidae)

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Journal of Coastal Life Medicine 2014年第3期 222-224页

作者： Marimuthu Govindarajan Mohan Rajeswary Unit of Vector Biology and Phytochemistry Department of ZoologyAnnamalai UniversityAnnamalainagarIndia

Objective:To assess the larvicidal potential of the crude benzene,chloroform,ethyl acetate and methanol solvent extracts of the medicinal plant Impatiens balsamina against anopheles stephensi(***),Aedes aegypti(***)and Culex quinquefasciatus(***).Methods:Twenty five third instar larvae of ***,*** and *** were exposed to various concentrations and were assayed in the laboratory by using the protocol recommended by *** larval mortality was observed after 24 h of ***:Among extracts tested,the highest larvicidal activity was observed in leaf methanol extract of Impatiens balsamina against ***,*** and *** with the LC_(50)and LC_(90)values 98.04,119.68,125.06 and 172.93,210.14,220.60 mg/L,***:From the results it can be concluded that the larvicidal effect of Impatiens balsamina against ***,*** and *** make this plant product promising as an alternative to synthetic insecticide in mosquito control programs.

关键词： Impatiens balsamina Larvicidal activity anopheles stephensi Aedes aegypti Culex quinquefasciatus

Mosquito repellent potential of Pithecellobium dulce leaf and seed against malaria vector anopheles stephensi(Diptera:Culicidae)

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Journal of Coastal Life Medicine 2016年第3期4卷 236-239页

作者： Mohan Rajeswary Marimuthu Govindarajan Unit of Vector Biology and phytochemistry Department of ZoologyAnnamalai UniversityAnnamalainagar-608002TamilnaduIndia

Objective:To determine the repellent properties of hexane,benzene,ethyl acetate,chloroform and methanol extract of Pithecellobium dulce(***)leaf and seed against anopheles stephensi(***).Methods:Repellent activity assay was carried out in a net cage(45 cm×30 cm×25 cm)containing 100 blood starved female mosquitoes of *** assay was carried out in the laboratory conditions according to the WHO 2009 *** crude extracts of *** were applied at 1.0,2.5,and 5.0 mg/cm^(2) separately in the exposed fore arm of study *** was used as the sole ***:In this study,the applied plant crude extracts were observed to protect against mosquito *** were no allergic reactions experienced by the study *** repellent activity of the extract was dependent on the concentration of the *** the tested solvents,the leaf and seed methanol extract showed the maximum *** highest concentration of 5.0 mg/cm^(2) leaf and seed methanol extract of *** provided over 180 min and 150 min protection,***:Crude extracts of *** exhibit the potential for controlling malaria vector mosquito ***.

关键词： Repellent activity Pithecellobium dulce Leaf Seed anopheles stephensi

Chemical composition and laboratory investigation of Melissa officinalis essential oil against human malarial vector mosquito,anopheles stephensi L.(Diptera:Culicidae)

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Journal of Coastal Life Medicine 2016年第12期4卷 969-973页

作者： Mathalaimuthu Baranitharan Shanmugam Dhansekaran Kadarkarai Murugan Kalimuthu Kovendan Jayapal Gokulakrishnan Division of Phytochemistry and Entomotoxicity Department of ZoologyAnnamalai UniversityAnnamalai Nagar 608002Tamil NaduIndia PG and Research Department of Zoology Thiru Kolanjiappar Government Arts CollegeVirudhachalam 606001Tamil NaduIndia Division of Entomology Department of ZoologySchool of Life SciencesBharathiar UniversityCoimbatore 641046Tamil NaduIndia Department of Zoology Poompuhar College(Autonomous)Melaiyur 609107Tamil NaduIndia

Objective:To decide the larvicides,ovicidal,pupicidal and repellent activity of Melissa officinalis(***)chemical compositions against important mosquito anopheles stephensi(***)(Diptera:Culicidae).Methods:A chemical constituent of 24 compounds was identified in the oils of *** compounds representing to 98.73%.A total of 253rd instar larvae of *** were showed to a variety of concentrations(30-300 mg/L)in laboratory by means of utilizing the standard procedure portrayed by World Health Organization(2005).The larvae were exposed for 24 h and mortalities were subjected to probit *** ovicidal activity was strong-minded against *** mosquito to a variety of concentrations ranging from 15-90 mg/L under the laboratory *** repellent activity of *** chemical compositions tested at concentrations of 0.75 and 1.50 mg/cm^(2)was evaluated in a net cage(45 cm×45 cm×40 cm)including 100 blood starving female mosquitoes of *** using the methods of World Health Organization(1996).Results:The LC_(50)and LC_(90)values of citronellal compound against *** larvae were 85.44 and 159.73 mg/L,*** percent hatchability of the ovicidal action was observed 48 h ***,the citronellal compound and other compositions were found to be mainly effective against eggs of *** compound exerted 45,60,75 and 90 mg/L against ***,*** repellent activity of citronellal compound was contained to be mainly effective and the maximum action was observed at 0.75 and 1.50 mg/cm^(2)concentrations giving 100%protection up to 210 min against ***:This current study was undertaken to evaluate the larvicidal,ovicidal,repellent potential of compounds from the *** essential oil against *** is initial statement on the mosquito larvicidal,ovicidal and repellent activity of *** chemical compositions.

关键词： Melissa ofifcinalis anopheles stephensi Larvicidal activity Ovicidal activity Repellent activity

食蟹猴疟原虫晚期卵囊、成孢子细胞与子孢子扫描电镜观察

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Current Zoology 1985年第3期31卷 232-235+301-302页

作者：陈佩惠吴洪张穗时云林郭保忠军事医学科学院微生物流行病研究所北京第二医学院微生物寄生虫学教研室

本研究对实验感染食蟹猴疟原虫后9、10和14天的斯氏按蚊阳性蚊胃,进行扫描电镜观察。晚期(分化的)卵囊表面呈现凹凸不平皱褶。成孢子细胞体常呈圆球形或椭圆形,表面光滑,子孢子芽从其表面长出。子孢子体细长,稍弯曲,体表光滑;虫体前部较... 详细信息

本研究对实验感染食蟹猴疟原虫后9、10和14天的斯氏按蚊阳性蚊胃,进行扫描电镜观察。晚期(分化的)卵囊表面呈现凹凸不平皱褶。成孢子细胞体常呈圆球形或椭圆形,表面光滑,子孢子芽从其表面长出。子孢子体细长,稍弯曲,体表光滑;虫体前部较细,顶端稍平,后部稍粗,末端钝圆。本文对成孢子细胞不同发育阶段的形态予以较详细描述,对子孢子出囊方式做了初步讨论。

关键词： Plamodium cynomolgi anopheles stephensi Ooeyst Sporozoite Scanning election microscopy (SBM).