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Screening and Identification of Strains Converting Trans-anethole to anisic acid

Agricultural Biotechnology 2021年第1期10卷 91-95页

作者： Jianglin YIN Xuejian LI Xueying ZHENG Yuyan FENG Jian ZHANG Guangxi University of Chinese Medicine Nanning 530200China

[Objectives] This study was conducted to isolate and screen the bacteria that can convert trans-anethole to anisic acid from star anise and its environmental samples, and identify the bacteria. [Methods] According to the traditional microbial culture method, with trans-anethole as the sole carbon source, through enrichment culture and separation and purification, preliminary screening by thin layer chromatography and re-screening by high-performance liquid chromatography, strains that degraded trans-anethole to produce anisic acid were obtained, and 16 S rDNA sequencing and phylogenetic tree construction were performed for genetic analysis. [Results] Eleven strains that degraded trans-anethole to produce anisic acid were obtained, among which strain NT2 that produced anisic acid with a relatively high efficiency was initially identified as Pseudomonas sp. The strain’s trans-anethole degradation rate was 45.41%, and the molar production rate and cumulative concentration of anisic acid were 21.80% and 1.96 g/L, respectively. [Conclusions] Strain NT2 has a strong ability to degrade trans-anethole to produce anisic acid, and can enrich strain resources for degradation of trans-anethole to anisic acid through microbial conversion.

关键词： Trans-anethole anisic acid Strain screening Microbial conversion

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Synthesis and Crystal Structure of Cadmium(Ⅱ) Complex [Cd(H_2O)(CH_3OC_6H_4COO)_2]_n

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Chinese Journal of Structural Chemistry 2002年第5期21卷 513-516页

作者：赵琦华马永平等 DepartmentofChemistry YunnanUniversityKunming650091China SchoolofPharmacy YunnanUniversityKunming650091China

At room temperature, the reaction of Cd(NO3)24H2O and anisic acid in an ethanol solution affords the cadmium (Ⅱ) complex [Cd(H2O)(CH3OC6H4COO)2]n. The crystal is of monoclinic, empirical formula C16H16O7Cd, Mr = 432.69, space group C2/c with parameters: a = 34.211(2), b = 6.030(2), c = 7.611(3) ? = 95.619(5)? V = 1562.5(9) ?, Z = 4, Dc = 1.831 g/cm3, = 1.434 mm-1, F(000) = 856, R = 0.0215 and wR = 0.0456. 1121 reflections with I ≥ 2s(I) were considered to be observed. Each cadmium atom is seven-coordinate in a distorted pentagonal bipyramidal geometry. The Cd (Ⅱ) center is doubly bridged with the neighboring Cd centers by anisate ligands to form a four-membered ring with a repeating unit of CdOCdO-. The extended structure and hydrogen-bonding patterns displayed in the complex were studied.

关键词： crystal structure polymeric complex cadmium complex anisic acid

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