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Multiplex lateral flow immunochromatographic assay for the qualitative and quantitative detection of six steroid hormone residues in chicken and pork

引用

FOOD BIOSCIENCE 2023年第3期53卷 341-350页

作者： Xu, Xiaoxin Guo, Lingling Xu, Liguang Kuang, Hua Xu, Chuanlai Liu, Liqiang Jiangnan Univ Sch Food Sci & Technol State Key Lab Food Sci & Technol Wuxi 214122 Jiangsu Peoples R China

To ensure food safety from animal origins and to prevent potential hazards caused by steroid hormones, there is an urgent need to develop a rapid detection method for steroid hormones in foods from animal origins. Three highly sensitive monoclonal antibodies (mAb) against testosterone propionate (Tp), stazolol (STA) and estradiol benzoate (EB) were prepared, with IC50 values of 0.13, 0.14 and 0.19 ng/mL, respectively. Tp-mAb is a group-selective mAb that simultaneously detects Tp, testosterone (T), methyltestosterone (MT) and testosterone phe-nylpropionate (TPP), and their IC50 values were 0.13, 0.77, 0.90, and 1.25 ng/mL, respectively. A novel multiplex lateral flow immunochromatographic strip (ICS) was further prepared, which could simultaneously detect Tp, STA, and EB in chicken and pork samples with limits of quantification (LOQ) of 0.32, 0.51, 0.35 mu g/kg and 0.36, 0.64, 0.43 mu g/kg, respectively. Based on the broad spectrum of Tp-mAb, ICS has been further devel-oped to detect Tp, T, MT and TPP simultaneously. This multiplex ICS rapid detection method was efficient, sensitive, and fast, for the simultaneous detection of multiple steroid hormones in actual samples. Its detection time is only 40 min, which it represents a favorable monitoring tool for the detection of steroid hormones in animal-derived foods from the marketplace.

关键词： Steroid hormones animal-derived foods Monoclonal antibodies Qualitative and quantitative assays

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