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Isolation,identification,characterization and antibiotic sensitivity profile of pathogenic Legionella pneumophila isolates from different water sources

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Asian Pacific Journal of Tropical Biomedicine 2017年第5期7卷 411-415页

作者： Kannan Subbaram Hemalatha Kannan Majed Mohammad Ahmad Masadeh Department of Medical Preparatory Year–Biology Al-Ghad International Colleges for Applied Medical Sciences(Male)RiyadhSaudi Arabia Department of Medical Laboratory Sciences&Pathology Jimma UniversityJimmaEthiopia Department of Pharmaceutical Technology Jordan University of Science and TechnologyIrbid 22110Jordan

Objective:To investigate the prevalence,isolation,identification,characterization,antibiotic profile and pathogenicity of Legionellae isolated from various set of ***:A total of 400 water samples were collected from different water *** samples were pretreated using acid treatment followed by concentration and culture on buffered charcoal yeast extract *** like ability of Legionella isolates to grow in various p H range,effect of different concentrations of chlorine and effect of different temperature optima were set *** tests were performed to separate Legionellae into *** sensitivity tests and test for pathogenicity were also conducted on isolated ***:The rates of isolation of Legionella pneumophila(***) in different water sources were found to be 20%(lakes),10%(ponds),8%(water-tanks) and 1%(rivers).Most of the isolates could grow in variable p H 6–8 and it could also survive the normal level of chlorination and even at temperature of 42 *** species of Legionellae resulted in identification of 5 different species,*** being the dominant *** of *** were resistant to many *** of Legionellae into intracerebral route of suckling mice revealed that *** was the most ***:Serious and fatal *** infections may be transmitted through *** can survive under various conditions in various water *** is the important pathogen causing human *** challenge prevails to health care professionals because these Legionellae acquired antibiotic resistance to many routinely prescribed antibiotics.

关键词： Legionella pneumophila Legionellae animal pathogenicity Virulence Water sources Antibiotic resistance

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