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The Partial Purification of angiogenesis factor of Human Osteosarcoma

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The Chinese-German Journal of Clinical Oncology 2002年第2期1卷 94-97页

作者： WUHua DENGZhongduan 等 DepartmentofOrthopedics TongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030 DepartmentofPathology SchoolofBasicMedicalSciencesTonjiMedicalColl

Objective To partially purify the angiogenesis factor of human osteosarcoma(HuOs) and study its biological features. Methods The active peptide with a molecular weight of 8000-10000 Da in the conditioned medium obtained from the cultivation of Hu-Os cells(osteoblastic osteosarcoma) was partially purified by ultrafiltration, chromatography and *** angiogenic effects of the fractions were assessed by proliferation assay of human umbilical vein and pig thoracic aorta endothelial cells. Results The chromatography fractions 4-6 could significantly promote the proliferation of the endothelial *** The HuOs cells could synthesize and secrete angiogenesis factor with a molecular weight of 8000-10000 Da.

关键词： osteosarcoma angiogenesis factor

Dietary rumen-protected L-arginine or N-carbamylglutamate enhances placental amino acid transport and suppresses angiogenesis and steroid anabolism in underfed pregnant ewes

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Animal Nutrition 2023年第4期15卷 149-158页

作者： Hao Zhang Xia Zha Bei Zhang Yi Zheng Xiaoyun Liu Mabrouk Elsabagh Yi Ma Hongrong Wang Guihua Shu Mengzhi Wang Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009China Joint International Research Laboratory of Agriculture and Agri-Product Safety the Ministry of Education of ChinaYangzhou UniversityYangzhou 225009China Department of Animal Production and Technology Faculty of Agricultural Sciences and TechnologiesNigde Omer Halisdemir UniversityNigde 51240Turkey Department of Nutrition and Clinical Nutrition Faculty of Veterinary MedicineKafrelsheikh UniversityKafrelSheikhEgypt Department of Pediatrics Northern Jiangsu People's HospitalClinical Medical CollegeYangzhou UniversityYangzhou 225001China

This study aimed to investigate the effects of dietary supplementation of underfed Hu ewes from d 35 to110 of gestation with either rumen-protected L-arginine(RP-Arg)or N-carbamylglutamate(NCG)on placental amino acid(AA)transport,angiogenic gene expression,and steroid *** d 35 of gestation,32 Hu ewes carrying twin fetuses were randomly divided into four treatment groups,each consisting of eight ewes,and were fed the following diets:A diet providing 100%of NRC’s nutrient requirements for pregnant ewes(CON);A diet providing 50%of NRC’s nutrient requirements for pregnant ewes(RES);RES diet plus 5 g/d NCG(RES+NCG);or RES diet plus 20 g/d RP-Arg(RES+ARG).On the d 110 of pregnancy,blood samples were taken from the mother,and samples were collected from type A cotyledons(COT;the fetal portions of the placenta).The levels of 17β-estradiol and progesterone in the maternal serum and both the capillary area density(CAD)and capillary surface density(CSD)in type A COT were decreased in response to Arg or NCG supplementation when compared to the RES *** concentrations of arginine,leucine,putrescine and spermidine in type A COT were higher(Pfactor 2(FGF2)were decreased in type A COT by supplementation with either NCG or RP-Arg compared to the RES *** results suggest that providing underfed pregnant ewes from d 35 to 110 of gestation with a diet supplemented with NCG or RP-Arg improves placental AA transport,and reduces the expression of angiogenic growth factor genes and steroid anabolism,leading to better fetal development.

关键词： angiogenesis factor L-arginine N-carbamylglutamate Placental amino acid transport Pregnant ewes Steroid anabolism

The effect of PHD2-shRNA genetransfection of H9C2 cells mediated by UTMD and Polyethylenimine on silencing the expression of PHD2 and increasing the expression of HIF-1α and its downstream angiogenesis factors

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The effect of PHD2-shRNA genetransfection of H9C2 cells medi...

PROGNOSTIC VALUE OF VEGF EXPRESSION IN PRIMARY ESOPHAGEAL SQUAMOUS CELL CARCINOMA

中国超声医学工程学会第三届全国介入超声医学学术会议

作者：张丽 Zhenxing Sun MingxingXie Department of Ultrasonography Union Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyHubei Province Key Laboratary of Molecular Imaging

Objective To study the effect of PHD2-shRNA genetransfectionofH9C2 cells mediated by ultrasound-targeted microbubblesdestruction(UTMD) and Polyethy-lenimine on silencing the expression of PHD2 and increasing the expression of HTF-1α and its downstream angiogenesis factors(VEGF,bFGF and TGF-β).Methods Culturenormoxia and anoxic H9C2 cells,and H9C2 cells are divided into four groups;normoxia H9C2 cells txansfected with EGFPplasmid(group A);normoxia H9C2 cells transfected with PHD2-shRNA plasmid(group B);anoxic H9C2 cells transfected with EGFPplasmid(groupC);anoxic H9C2 cells transfected with PHD2-shRNA plasmid(group D).Set irradiation optimal parameter which was obtained before this study(ultrasonic intensity;1.5 W/cm2,microbubbles concentration;30%,exposure time:30 sand plasmid concentration:16μg/ml) The PHD2 shRNA vector was transfected into H9C2 cells with thereporter vector H1-MCS-CMV-GFP-SV40-Neomycin that contained GFPdriven by the CMV *** 48 houra of the PHD2-shRNA and EGFP plasmid genetransfection of H9C2 cells mediated by ultrasound-targeted microbubblesdestruction and Polyethylenimine,observe green fluorescent protein with inverted flurescence *** protein expression of PHD2,HTF-1α,VEGFand bFGFwere tested by Western-blot and the mRNA expression of PHD2,HIF-1α,VEGF and bFGF were measured by *** The green fluorescent proteinwere visible in four groups after H9C2 cells were transfected for 48 *** intensity of green fluorescent protein in four groups had no difference(figure 1).RT-PCR shown that the PHD2 RNAexpression of the group B waslesser than the group A and group D lesser than group ***,HIF-1α,VEGF,bFGF and TGF-βRNA expression were precisely the *** differences were statistically significant(figure 2).The western-blot shown that the PHD2,HIF-1α,VEGF,bFGF and TGF-βprotein expression were in line with corresponding RNA expression(figure 3).Conclusion The PHD2-shRNA genetransfectionof H9C2 cells mediated by

关键词： UTMD Gene transfection angiogenesis factor

来源： cnki会议评论

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Chinese Journal of Cancer Research 2004年第2期16卷 85-89页

作者：徐卫国杨国利ボ立新谢玉泉张力建 Department of Surgical Oncology Peking University School of Oncology Beijing 100036

To study the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in esophageal squamous cell carcinoma (ESCC) and clarify the association of VEGF expression with the angiogenesis and prognostic value of this disease. Methods: Eighty-two cases with primary ESCC treated with radical operation in Department of Surgery from Jan 1981 through May 1994 were enrolled. VEGF expression and MVD value were examined by immunohistochemical staining, the streptavidin-biotin peroxidase complex method (SP method), using anti-VEGF polyclonal antibody and anti-factor-VIII antibody, respectively. We also analyzed the relationship between VEGF expression and MVD value and postoperative survival rate of patients. Results: Of the 82 cases, 63.4% cases showed positive for VEGF in tumor cells and the median of MVD in tumor was 37 (9-150)·mm-2. There was a close correlation between MVD and VEGF (P=0.001). The 5-year survival rate of patients with low and high MVD was 34.1% and 12.2%, respectively. The 5-year survival rate was 46.7% in patients with VEGF-negative tumor and 11.5% in patients with VEGF-positive tumor. These differences were statistically significant (P=0.017 and Pangiogenesis is mediated mainly by VEGF and VEGF may be associated with tumor progression and increased malignancy via angiogenesis.

关键词： Esophageal Squamous cell Carcinoma angiogenesis factor Biosynthesis Neovascularization Prognosis

Expression of angiogenic factors in cerebral arteriovenous malformations

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Neural Regeneration Research 2007年第3期2卷 138-141页

作者： Mingguang Zhao Youli Chen Zhenquan Song Yongzhong Gao Peiyu Pu Xuezhong Wei Department of Neurosurgery General Hospital of Shenyang Military Area Command of Chinese PLA Shenyang 110016 Liaoning Province China Department of Neurosurgery General Hospital of Tianjin Medical University Tianjin 300052 China

BACKGROUND： In the process of vascularization, vascular endothelial growth factor （VEGF）, angiopoietin-2 and Tie2 are involved in the migration, differentiation and proliferation of vascular endothelial cells, and stimulate the rapid angiogenesis; Tiel and angiopoietin-1 play important roles in facilitating the formation of vascular lumen and maintaining the integrity of vascular wall. Thus the distributions and expressions may be associated with the occurrence of cerebral arteriovenous malformation. OBJECTIVE： To observe the biological effects of angiogenic factors in the occurrence and development of cerebral arteriovenous malformation. DESIGN： An observational comparative experiment. SETTINGS： Department of Neurosurgery, General Hospital of Shenyang Military Area Command of Chinese PLA; Department of Neurosurgery, General Hospital of Tianjin Medical University. PARTICIPANTS： Fresh samples of complete cerebral arteriovenous malformations resected in 47 patients were collected from the Department of Neurosurgery, General Hospital of Tianjin Medical University from August 1999 to May 2001, including 22 males and 25 females, the mean age was 34.5 years. Informed consents were obtained from all the patients or their relatives. The initial symptom was hemorrhage in 28 cases. All the patients were classified according to the clinical imaging data and Spetzler-Martin grading standard, including 11 cases of grade Ⅰ, 17 cases of grade Ⅱ, 11 cases of grade Ⅲ, and 8 cases of grade Ⅳ - Ⅴ. Normal brain tissues resected by decompression due to trauma were taken from 8 patients as controls, including 5 males and 3 females, aging 12 - 65 years. METHODS： ① The expressions of VEGF, Tie receptors, angiopoietin-1, angiopoietin-2, proto-oncogene c-myc and proliferating cell nuclear antigen（PCNA） in the samples of cerebral arteriovenous malformation were detected with immunohistochemical method. Under light microscope, the positively stained rat-anti-human factor Ⅷ-related antigens （sp

关键词： cerebral arteriovenous malformations angiogenesis factor proto-oncogenes proliferating cell nuclear antigen

Priming of the Cells： Hypoxic Preconditioning for Stem Cell Therapy

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Chinese Medical Journal 2017年第19期130卷 2361-2374页

作者： Zheng Z Wei Yan-Bing Zhu James Y Zhang Myles R McCrary Song Wang Yong-Bo Zhang Shan-Ping Yu Ling Wei Department of Neurology Beijing Friendship Hospital Capital Medical University Beijing 100050 China Experimental and Translational Research Center Beijing Friendship Hospital Capital Medical University Beijing 100050 China Department of Anesthesiology Emory University School of Medicine Atlanta Georgia 30322 USA

Objective： Stem cell-based therapies are promising in regenerative medicine for protecting and repairing damaged brain tissues after injury or in the context of chronic diseases. Hypoxia can induce physiological and pathological responses. A hypoxic insult might act as a double-edged sword, it induces cell death and brain damage, but on the other hand, sublethal hypoxia can trigger an adaptation response called hypoxic preconditioning or hypoxic tolerance that is of immense importance for the survival of cells and tissues. Data Sources： This review was based on articles published in PubMed databases up to August 16, 2017, with the following keywords：＂stem cells,＂＂hypoxic preconditioning,＂＂ischemic preconditioning,＂ and ＂cell transplantation.＂Study Selection： Original articles and critical reviews on the topics were selected. Results： Hypoxic preconditioning has been investigated as a primary endogenous protective mechanism and possible treatment against ischemic injuries. Many cellular and molecular mechanisms underlying the protective effects of hypoxic preconditioning have been identified. Conclusions： In cell transplantation therapy, hypoxic pretreatment of stem cells and neural progenitors markedly increases the survival and regenerative capabilities of these cells in the host environment, leading to enhanced therapeutic effects in various disease models. Regenerative treatments can mobilize endogenous stem cells for neurogenesis and angiogenesis in the adult brain. Furthermore, transplantation of stem cells/neural progenitors achieves therapeutic benefits via cell replacement and/or increased trophic support. Combinatorial approaches of cell-based therapy with additional strategies such as neuroprotective protocols, anti-inflammatory treatment, and rehabilitation therapy can significantly improve therapeutic benefits. In this review, we will discuss the recent progress regarding cell types and applications in regenerative medicine as well as future applicatio

关键词： angiogenesis factor Cell Transplantation Endogenous Stem Cells Genome Editing Hypoxia Hypoxic Preconditioning Induced Pluripotent Stem Cells Neurological Disorders Tumor

Experimental study on angiogenesis in rabbit VX_2 brain tumor using perfusion CT

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The Chinese-German Journal of Clinical Oncology 2006年第6期5卷 431-435页

作者： Liqing Kang Yunting Zhang Shimei Sun Department of Radiology Cangzhou Central Hospital Cangzhou 061001 China Department of Radiology General Hospital of Tianjin Medical University Tianjin 300052 China Department of Oncology Cangzhou Central Hospital Cangzhou 061001 China

Objective： To study the perfusion CT features of rabbit VX2 brain tumor with correlation to MVD and VEGF, and to validate perfusion CT for reflection of tumor angiogenesis. Methods： Rabbit VX2 brain tumor model was established by injection of 100 μL viable tumor cells （10qmL） through a 2 mm-hole 5 mm to the right of the sagittal suture and 5 mm posterior to the coronal suture bored by dental drill. MRI was performed every 2 days after seven days of implantation to evaluate the growth of the tumor. Twenty New Zealand White rabbits with tumor size over 3 mm in diameter were randomly divided into 2 groups according to the tumor growth time with those less than 3 weeks as group 1 and those more than 3 weeks as group 2, and perfusion CT were performed accordingly. CT measurements of BV, BF and PS from tumor, peritumor and contralateral normal tissue regions were obtained. After that the animals were sacrificed and 2% Evans blue （2 mL/kg） was given intravenously in 16 of these animals 1 h prior to sacrifice to detect breakdown of the blood brain barrier. VEGF and MVD were evaluated in immunohistochemical examination of the specimens. Results： Tumor had significantly higher BV, BF and PS （P=0.000） than peritumor and normal tissue region. Tumor BV, BF and MVD in group 2 were significantly higher than that in group 1 （P〈0.01）. Significant linear correlation was found between MVD and BV （t=-0.915, P=-0.000）, MVD and BF （t=0.901, P=-0.000）, and MVD and PS （t=-0.459, P=0.042）. We also found a rank correlation between PS and blue stain of tumor （rs=0.861, P=0.000）. Conclusion： Perfusion CT can distinguish tumor from peritumor and normal tissue clearly, reflect tumor angiogenesis accurately, and provide useful information for the evaluation of brain tumor.

关键词： brain neoplasms angiogenesis factor perfusion tomography, X-ray computed