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Journal of Huazhong University of Science and Technology(Medical Sciences) 2011年第4期31卷 530-534页

作者：刘双林王志华胡志全曾星李有元苏耀武章传华叶章群 Department of Urology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Urology Wuhan First Hospital Department of Urology Beilun People's Hospital

The anti-tumor activity of curcumin against androgen-independent prostate cancer cells in vitro and the possible mechanism were *** curcumin treatment,the effect of curcumin on the proliferation of prostate cancer PC-3 cells was assessed by CFSE *** cytometery（FCM） was performed to analyze the cell cycle and the induction of apoptosis of tumor cells.A luciferase reporter gene assay was used to determine the effects of curcumin on the activities of intracellular NF-κB and AP-1 signaling *** results showed curcumin could effectively inhibit the proliferation of PC-3 cells in vitro（P0.05）.Cells were arrested at G2/M *** curcumin treatment,the percentage of apoptotic cells was significantly higher than in control group（P0.05）.The results of the luciferase assay revealed that curcumin selectively inhibited the activities of the NF-κB and AP-1 signaling pathways in PC-3 cells *** was suggested that curcumin could exert anti-tumor activity against androgen-independent prostate cancer cells in vitro by inhibiting cellular proliferation and inducing apoptosis,which was probably contributed to the inhibition of transcription factors NF-κB and AP-1.

关键词： curcumin androgen-independent prostate cancer NF-κB AP-1

Identification of commonly regulated protein targets and molecular pathways in PC-3 and DU145 androgen-independent human prostate cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one

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Asian Pacific Journal of Tropical Biomedicine 2018年第9期8卷 436-445页

作者： Kamini Citalingam Faridah Abas Nordin H.Lajis Iekhsan Othman Rakesh Naidu Jeffery Cheah School of Medicine and Health Sciences Monash University Malaysia Jalan Lagoon Selatan Laboratory of Natural Products Faculty of Science Universiti Putra Malaysia Department of Food Science Faculty of Food Science and Technology Universiti Putra Malaysia

Objective: To identify mutually regulated proteins in PC-3 and DU145 androgen-independent prostate cancer cell lines treated with 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one(MS17), and to study the molecular pathways that contributed to the anticancer activity of ***: PC-3 and DU145 cells were treated with 3 × EC_(50)(15 μM) concentration of MS17 for 24 h and were subjected to protein expression profiling using two-dimensional gel electrophoresis and protein identification by mass *** differentially expressed proteins with significant P-value of Pandrogen-independent prostate cancer.

关键词： androgen-independent prostate cancer Diarylpentanoid Proteomics profiling Mass spectrometry Pathway analysis

The novel long noncoding RNA LOC283070 is involved in the transition of LNCaP cells into androgen-independent cells via its interaction with PHB2

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Asian Journal of Andrology 2018年第5期20卷 511-517页

作者： Ying Zhang Li-Na Wang Ya-Ni Lin Yuan-Xin Xing Yu Shi Jian Zhao Wei-Wen Chen Bo Han Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Cheeloo College of Medicine Shandong University Jinan 250012 China Department of Clinical Laboratory Medicine The Second Hospital of Shandong University Jinan 250033 China School of Medicine Cheeloo College of Medicine Shandong University Jinan 250012 China Department of Thoracic Surgery Qilu Hospital of Shandong University Jinan 250012 China Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Pathology School of Basic Medical Sciences Cheeloo College of Medicine Shandong University Department of Pathology Qilu Hospital of Shandong University Jinan 250012 China

We sought to investigate the underlying mechanism of action of the long noncoding RNA （IncRNA） LOC283070 in the development of androgen independence in prostate cancer. The interactions between LOC283070 and target proteins were investigated by RNA pull-down and RNA-binding protein immunoprecipitation （RIP） assays. Subceilular fractionation and quantitative reverse transcription-polymerase chain reaction （qRT-PCR） were used to detect the subcellular localization of LOC283070. Western blotting was performed to detect the expression of prohibitin 2 （PHB2）. Luciferase activity assays were performed to evaluate the effects of LOC283070 and PHB2 on the androgen receptor （AR） signaling pathway. A methyl thiazolyl tetrazolium （MTT） assay and a growth curve assay were used to test cell viability. Flow cytometry was performed to analyze cell cycles. A transwell assay was employed to test cell migration. We identified PHB2 as an interaction partner of LOC283070 in the pull-down and RIP experiments. Furthermore, we confirmed that the enrichment of L0C283070 with PHB2 in androgen-independent LNCaP （LNCaP-AI） cells was much greater than that in LNCaP cells. Moreover, the expression of PHB2 was not significantly different between the two cell lines, and the expression of LOC283070 in the nuclei of the LNCaP-AI cells was significantly greater than that in the LNCaP cells. In vitro data revealed that PHB2 overexpression significantly inhibited AR activity and cell proliferation and migration and induced accumulation of prostate cancer cells in GO/G1 phase. Moreover, the overexpression of LOC283070 fully abrogated the effects of PHB2 overexpression. In conclusion, we found that LOC283070 can bind to PHB2 located in the nucleus and inhibit its effect, and this is one of the mechanisms by which LOC283070 is involved in the transition of LNCaP cells into androgen-independent cells.

关键词： androgen-dependent prostate cancer androgen-independent prostate cancer LOC283070 long noncoding RNA prohibitin 2

NES1/KLK10 and hNIS gene therapy enhanced iodine-131 internal radiation in PC3 proliferation inhibition

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Frontiers of Medicine 2019年第6期13卷 646-657页

作者： Jiajia Hu Wenbin Shen Qian Qu Xiaochun Fei Ying Miao Xinyun Huang Jiajun Liu Yingli Wu Biao Li Department of Nuclear Medicine Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of Pathology Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Hongqiao International Institute of Medicine Shanghai Tongren Hospital/Faculty of Basic MedicineChemical Biology Division of Shanghai Universities E-InstitutesKey Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of EducationShanghai Jiao Tong University School of MedicineShanghai 200025China

NES1 gene is thought to be a tumor-suppressor *** previous study found that overexpression of NES1 gene in PC3 cell line could slow down the tumor proliferation rate,associated with a mild decrease in BCL-2 *** BCL-2 decrease could increase the sensitivity of radiotherapy to ***,we supposed to have an“enhanced firepower”effect by combining overexpressed NES1 gene therapy and 131I radiation therapy uptake by overexpressed hNIS *** found a weak endogenous expression of hNIS protein in PC3 cells and demonstrated that the low expression of hNIS protein in PC3 cells might be the reason for the low iodine *** overexpressing hNIS in PC3,the radioactive iodine uptake ability was significantly *** of in vitro and in vivo tumor proliferation experiments and 18F-fluorothymidine(18F-FLT)micro-positron emission tomography/computed tomography(micro-PET/CT)imaging showed that the combined NES1 gene therapy and 131I radiation therapy mediated by overexpressed hNIS protein had the best tumor proliferative inhibition *** showed an obvious decrease of Ki-67 expression and the lowest BCL-2 *** data suggest that via inhibition of BCL-2 expression,overexpressed NES1 might enhance the effect of radiation therapy of 131I uptake in hNIS overexpressed PC3 cells.

关键词： androgen-independent prostate cancer normal epithelial cell-specific 1/kallikrein 10 sodium/iodide symporter radiation therapy proliferation

Emerging role of transforming growth factor-β-regulated long non-coding RNAs in prostate cancer pathogenesis

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cancer Pathogenesis and Therapy 2023年第3期1卷 195-204页

作者： Bakhya Shree Koyel Das Vivek Sharma Department of Biological Sciences Birla Institute of Technology and Science PilaniHyderabad CampusHyderabad 500078India

prostate cancer(PCa)is the most common malignancy in *** aggressive therapy involving surgery and hormonal treatments,the recurrence and emergence of metastatic castration-resistant prostate cancer(CRPCa)remain a major *** of the transforming growth factor-β(TGF-β)signaling pathway is crucial to PCa development and *** also contributes to androgen receptor activation and the emergence of *** addition,TGF-β signaling regulates long non-coding RNA(lncRNA)expression in multiple cancers,including ***,we discuss the complex regulatory network of lncRNAs and TGF-β signaling in PCa and their potential applications in diagnosing,prognosis,and treating *** investigations on the role of lncRNAs in the TGF-β pathway will help to better understand PCa pathogenesis.

关键词： prostate cancer TGF-β LncRNA Castration-resistant prostate cancer androgen deprivation therapy androgen-independent prostate cancer prostate-specific antigen Benign prostatic hyperplasia