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Bioinspired Andrias davidianus-Derived wound dressings for localized drug-elution

Bioactive Materials 2022年第9期7卷 482-494页

作者： Xiang Liu Xiang Mao Guo Ye Menghong Wang Ke Xue Yan Zhang Hongmei Zhang Xiaoqiao Ning Man Zhao Jinlin Song Yu Shrike Zhang Ximu Zhang Chongqing Key Laboratory of Oral Disease and Biomedical Sciences and Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education and Stomatological Hospital Chongqing Medical UniversityChongqing401174China State Key Laboratory of Ultrasound in Medicine and Engineering and Chongqing Key Laboratory of Biomedical Engineering College of Biomedical EngineeringChongqing Medical UniversityChongqing400016China Department of Stomatology The Third Affiliated HospitalChongqing Medical UniversityChongqing401120China Department of Plastic and Reconstructive Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of Medicine639 Zhi Zao Ju RdShanghai200011China Department of Pharmacy The 958th Hospital of PLAChongqing404100China Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolCambridgeMA02139USA Department of Plastic and reconstructive surgery Hainan Western Central HospitalHaiNan571700China

Local drug delivery has received increasing attention in recent years.However,the therapeutic efficacy of local delivery of drugs is still limited under certain scenarios,such as in the oral cavity or in wound beds after resection of tumors.In this study,we introduce a bioinspired adhesive hydrogel derived from the skin secretions of Andrias davidianus(SSAD)as a wound dressing for localized drug elution.The hydrogel was loaded with aminoguanidine or doxorubicin,and its controlled drug release and healing-promoting properties were verified in a diabetic rat palatal mucosal defect model and a C57BL/6 mouse melanoma-bearing model,respectively.The results showed that SSAD hydrogels with different pore sizes could release drugs in a controllable manner and accelerate wound healing.Transcriptome analyses of the palatal mucosa suggested that SSAD could significantly upregulate pathways linked to cell adhesion and extracellular matrix deposition and had the ability to recruit keratinocyte stem cells to defect sites.Taken together,these findings indicate that property-controllable SSAD hydrogels could be a promising biofunctional wound dressing for local drug delivery and promotion of wound healing.

关键词： Andrias davidianus Hydrogel Drug loading Controlled drug delivery Stem cell recruitment Wound healing

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Nutritional and medicinal characteristics of Chinese giant salamander (Andrias davidianus) for applications in healthcare industry by artificial cultivation: A review

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Food Science and Human Wellness 2018年第1期7卷 1-10页

作者： Dong He Wenming Zhu Wen Zenga Jun Lin Yang Ji Yi Wang Chong Zhang Yuan Lu Daoquan Zhao Nan Su Xin-Hui Xing Key Laboratory for Industrial Biocatalysis Ministry of EducationInstitute of Biochemical EngineeringDepartment of Chemical EngineeringRoom 601Yingshi BuildingBeijing100084China Centre for Synthetic and Systems Biology Tsinghua UniversityBeijing100084China Henan Academy of Aquatic Sciences No.38 Jiangshan RoadZhengzhouHenan450001China

Andrias davidianus,i.e.Chinese giant salamander(CGS),is one of the largest and oldest amphibians existing in the world and is also one of the valuable biological resources of China.Wild CGS has been threatened with extinction in the past decades due to over capturing,deterioration of natural environment,the slow breeding and growth of the wild species in nature.However,in the past twenty years,with the breakthrough and progress of artificial breeding technology by artificial insemination,the number of artificially cultivated CGS has increased rapidly.Artificially cultivated CGS can either be released to the CGS living environment to increase the population in nature or legally applied in food and medicinal industry as a feedstock due to the unique nutritional and medicinal values of CGS as recorded historically.In this review,the nutritional components,bioactive components and medicinal activities of the artificially cultivated CGS will be summarized.The mucus,skin,meat and bone of CGS contain many different bioactive substances thereby having various medicinal activities including anti-aging,anti-fatigue,anti-tumor,therapy of burn and anti-infection and other physiological functions.This paper will further discuss the potential applications of the artificially cultivated CGS in healthcare industry and prospects of future technological development.

关键词： Andrias davidianus Artificial breeding Chinese giant salamander Functional foods Medicinal activity Natural resource protection Nutrition

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Pathological Changes in Andrias davidianus Infected with Chinese Giant Salamander Ranavirus

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Asian Herpetological Research 2016年第4期7卷 258-264页

作者： Yi GENG Matthew James GRAY Kaiyu WANG Defang CHEN Ping OUYANG Xiaoli HUANG Changliang HE Zhijun ZHONG Zexiao YANG College of Veterinary Medicine Sichuan Agricultural University Center for Wildlife Health University of Tennessee Department of Aquaculture Sichuan Agricultural University

Chinese giant salamander ranavirus （CGSRV） is an emerging pathogen in captive populations of the Chinese giant salamander （Andrias davidianus）. We processed 140 morbid Chinese giant salamanders from seven captive breeding populations over five years, and describe the disease associated with CGSRV infection. The most common gross signs were significant swelling of the legs and coelomic cavity, erythema of the legs and ventrum in juveniles; cutaneous erosions and ulcerations in adults, particularly the limbs and the head; and marked petechial or ecchymotic hemorrhages of the internal organs, particularly the liver, spleen and kidney. Histological examination showed degeneration, necrosis, and inflammation in many organs, particularly in the organs where hemorrhage was observed. There was evidence of eosinophilic inclusion bodies in degenerated and necrotic cells. We identified virus particles and empty capsids without viral nucleoid in the inclusion bodies using electron microscopy. Virus particles were hexagonal or round shape, and appeared in paracrystalline arrays, aggregates, or singly. All enveloped viral particles were 140-160 nm. Polymerase chain reaction followed by sequencing verified that the virus particles were CGSRV. These results collectively support that CGSRV was the etiologic agent responsible for these mass die-offs of the Chinese giant salamander. The pathology described herein will be useful in diagnosing cases of ranaviral disease caused by CGSRV, and provide evidence that this pathogen is a significant threat to the Chinese giant salamander.

关键词： pathology CGSRV Andrias davidianus ranavirus China

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Embryonic development and organogenesis of Chinese giant salamander,Andrias davidianus

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Progress in Natural Science:Materials International 2007年第11期17卷 1303-1311页

作者： Luo Jian, Xiao Yamei, Luo Kaikun, Huang Xiaoxi, Peng Liangyue, Liu Yun(Key Laboratory of Protein Chemistry and Fish Developmental Biology of National Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China) Key Laboratory of Protein Chemistry and Fish Developmental Biology of National College of Life Sciences Hunan Normal University Changsha 410081 China

The morphology and organogenesis of Chinese giant salamander, Andrias davidianus, in its different developmental periods and stages are described in detail, which provides an intact criterion for distinguishing different stages of its developmental process. Based on the external morphological and internal histological features, six periods including 20 stages of organogenesis of Chinese giant salamander are established, which are cleavage period, blastula period, gastrula period, neurula period, organogenesis stage and hatching stage. Generally, the embryonic development of Chinese giant salamander is consistent with those of Eastern newt, Cynops orientalis, and Black spots frog, R. nigromaculata. However, they have some differences in the early cleavage process and the development of digestive system. The cleavage of Chinese giant salamander, A. davidianus is not a discoidal division type, which is different from other species reported. And the first three cleavages being meridional and a retardant development of its digestive system without halter and sucker existing are the evident features of the embryonic development of Chinese giant salamander.

关键词： Chinese giant salamander Andrias davidianus embryogenesis cleavage organogenesis

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Isolation and Properties of Collagen from the Skin of Andrias davidianus

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Animal Husbandry and Feed Science 2014年第6期6卷 319-321,324页

作者： Li Hua Deng Lin Sichuan Technology and Business College

[ Objective] To explore and utilize the collagen from the skin of bred Andrias davidianus. [ Method] Collagen was isolated with enzymolysis technology at 4℃, and the compositions and some biochemical properties were studied. [ Result] The content of the hydroxyproline in the skin ofA. davidianus was 22.3 %, the collagen extraction ratio was 26.7% （ based on wet weight）, the contents of the moisture, crude ash, crude fat, and crude protein were 6.75%, 0.62%, 0.38% and 92.10%, respectively. The collagen contained 8 essential amino acids for adult and 2 essential amino acids for children, the contents of the imlno acid was 171 residues/1 000 residues, and hydroxylation of proline was 51.5%. As ultraviolet （UV） absorption spectrum showed, the strong absorption peak was near 230 nm, according to the SDS - PAGE, it consisted of two diffcremt α chains （ αl and α2） which was characterized to be type I collagen. [ Conclusion] Extraction of collagen from the skin of bred A. davidianus is feasible theoretically, the extraction rate and purity arc very high.

关键词： Andrias davidianus Skin Collagen Isolation Property

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Development of an eDNA metabarcoding tool for surveying the world’s largest amphibian

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Current Zoology 2022年第5期68卷 608-614页

作者： Jie Wang Ping Liu Jiang Chang Cheng Li Feng Xie Jianping Jiang CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041Chinaand State Key Laboratory of Environmental Criteria and Risk AssessmentChinese Research Academy of Environmental SciencesBeijing 100012China

Due to the overexploitation of farming,as well as habitat destruction,the wild population of Chinese giant salamander(CGS)Andrias davidianus,a species with seven genetically distinct lineages,has decreased by over 80%in the past 70 years.Traditional survey methods have proven to be unsuitable for finding this rare and elusive species.We evaluated the efficacy of environmental DNA(eDNA)sampling to detect CGS indirectly from its aquatic environment.We developed several species-specific primer sets;validated their specificity and sensitivity;and assessed their utility in silico,in the laboratory,and at two field sites harboring released farm-bred CGS.We detected the presence of CGS DNA by using polymerase chain reaction and Sanger sequencing.We also sequenced an amplicon mixture of seven haplotype-represented samples using high-throughput sequencing.Our eDNA methods could detect the presence of CGS at moderate densities reported across its range,proving them as a cost-effective way to establish broad-scale patterns of occupancy for CGS.In addition,our primers enabled the detection of mitochondrial lineage mixture or introduced individuals from geographically isolated populations of CGS.

关键词： Andrias davidianus Chinese giant salamander eDNA metabarcoding population survey

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Efficient scarless skin regeneration enabled by loading micronized amnion in a bioinspired adhesive wound dressing

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Aggregate 2023年第5期4卷 96-111页

作者： Huicong Zhang Mingyu Song Changhong Hu Zhiyi Zhang Shuqi Zhang Yan Zhang Yingming Yang Puxu Zhou Liwen Zheng Lan Li Mengjie Mao Yu Shrike Zhang Ping Ji Ximu Zhang Chongqing Key Laboratory of Oral Disease and Biomedical Sciences and Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education and Stomatological Hospital of Chongqing Medical University ChongqingChina Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolCambridgeMassachusettsUSA Department of Preventive Dentistry State Key Laboratory of Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengduChina School of Stomatology Zunyi Medical UniversityZunyiChina

Complete skin reconstruction is a hierarchical,physiological assembly process involving healing of the epidermis,dermis,vasculature,nerves,and cutaneous appendages.To date,few works have reported complete skin regeneration,particularly lacking vascular structures and hair follicles after full skin defects.In this study,a hydrogel derived from the skin secretion of Andrias davidianus(SSAD)that features adhesiveness was used as a bioactive scaffold to load micronized amnion(MA).The SSAD hydrogel was found to promote the migration and proliferation of amnion stem cells and human keratinocytes,as well as inhibit their apoptosis in vitro.In a rat full-skin defect model,the regeneration of skin appendages was observed at the wound area,achieving scarless healing.Transcriptome analyses further validated that SSAD could positively regulate cell migration,proliferation,and differentiation.These functions might be attributed to the abundant growth factors present in the SSAD.Synergized by the delivery of MA,SSAD loaded with the MA could achieve a significantly better skin regeneration effect than SSAD or MA used alone,providing a simple yet highly effective means to obtain complete,scarless skin regeneration,suggesting favorable potential for clinical translation.

关键词： amnion Andrias davidianus cell delivery cutaneous appendages scarless wound dressing

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Genetic Bottlenecks of the Wild Chinese Giant Salamander in Karst Caves

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Asian Herpetological Research 2017年第3期18卷 174-183页

作者： Jie WANG Hongxing ZHANG Feng XIE Gang WEI Jianping JIANG Chengdu Institute of Biology Chinese Academy of Sciences Shaanxi Institute of Zoology Guiyang University

The rarity of limestone cave species due to habitat degradation makes them of special interest in conservation biology.The wild Chinese Giant Salamander Andrias davidianus,an evolutionarily distinct and globally endangered species,are nearly all obligate into living in inaccessible mountain caves now.We detected that only 14-29 breeders,with the effective population size of 9-25（5-44 of 95% confidence interval）,were in each of three caves,through genotyping 20 microsatellite loci on larvae that were flushed out of caves.Both heterzygosity excess and M（ratios of allele number to allele size range） tests indicated severe genetic bottlenecks among populations.Both mitochondrial,with only one or two haplotypes of D-loop region（770-771bp） in each population,and nuclear genetic structure showed clear divergence between populations.Considering the long life history,small population size,and genetic differentiationof the Chinese Giant Salamander,putting an end to poaching and recovering the karst ecosystem instead of releasing may be the sole measures to save this severely threated species.

关键词： limestone cave small population genetic drift Andrias davidianus subterranean habitat

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Chromosome G-banding in SituHybridization of RFLP Marker umc58 Linked with the Gene hm1 Dictating Helminthosporium carbonum Susceptibility1 in Maize

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Wuhan University Journal of Natural Sciences 1997年第2期2卷 99-103页

作者： Deng Fengjiao Feng Xiaorong Zheng Junying Liu Ting Yi Qingming Zhang Xiyuan Xiao Hanbing Xue Zhigang

Two set of andrias davidanus fertilized eggs and its seedlings were got by means of artificial reproduction. The sections of the embryo of Andrias davidianus were made by histological method. The expression of homologous sequences of Quox 1 gene has been studied by immunohistochemical method with special antibody. Results show that the expression of homologous sequences of Quox 1 gene was detected in the epidermis of blastula and gastula.The highly expression was investigated in the epidermis at the neural tube phrase and the tail bud stage.

关键词： Quox 1 gene Andrias davidianus embryo development immunohistochemical method

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