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Biochar-Bacillus consortium for a sustainable agriculture:physicochemical and soil stability analyses

Biochar 2023年第1期5卷 319-333页

作者： Aaronn Avit Ajeng Rosazlin Abdullah Tau Chuan Ling Institute of Biological Sciences Faculty of ScienceUniversity of Malaya50603 Kuala LumpurMalaysia Centre for Research in Biotechnology for Agriculture(CEBAR) Institute If Biological SciencesFaculty of ScienceUniversity of Malaya50603 Kuala LumpurMalaysia

Biochar has various agricultural applications,including the promising use as a carrier for beneficial ***,most recent research has demonstrated the possible attachment or immobilization of a single bacterial species onto biochar rather than a consortium of microbes for biotechnological ***,an assessment on the potential of oil palm kernel shell(OPKS)biochar as a biofilm-producing Bacillus consortium carrier through optimization study on the operating and environmental factors influencing the biofilm adhesion was conducted using response surface methodology(RSM)and the subsequent soil stability and storage potential of the *** highest Bacillus population was observed at temperature 33°C,agitation speed of 135 rpm,at a neutral pH of 7.5 with 10%(w/w)of sago starch as the co-carbon *** adhesion of Bacillus on OPKS biochar following the optimized conditions fitted pseudo-second order(PSO)of kinetic modelling(R^(2)=0.998).The optimized formulation was subjected to storage in different temperatures and in vitro soil incubation which revealed that the Bacillus biofilmadhered OPKS biochar may be stored up to 4 months with minimum range of live Bacillus viability reaching 107 CFU g^(-1) of biochar which is within the minimum range of acceptable biofertilizer viability(106 CFU mL^(-1)).Formulation that is viable in room storage can be easily incorporated into current agricultural distribution networks that do not have *** work highlighted the physicochemical and soil stability qualities of optimized Bacillus consortium adhesion on biochar for agricultural usage.

关键词： Process optimization Biochar Plant growth promoting bacteria Adhesion kinetics and isotherms

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