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Study on parameters influencing analytical performance of laser-induced breakdown spectroscopy

Frontiers of physics 2012年第6期7卷 714-720页

作者： Yong Zhang Yun-Hai Jia Jin-Wen Chen Xue-Jing Shen Lei Zhao Chun Yang Yong-Yan Chen Yong-Hui Zhang Peng-Cheng Han National Analysis Center for Iron and Steel Central Iron&Steel Research InstituteBeijing 100081China

Lens-to-sample distances, delay time, atmospheric condition, laser pulse energy, etc. had obvious effects on the analytical performance of laser-induced breakdown spectroscopy. In this paper, these parameters are investigated in greater detail and we will explain how they have influences on the analytical performance. The results show that the focal plane under the sample surface can improve precision and detection limit, and the delay time should be decided according to sensitivity and accuracy. Spectral line intensity is stronger in argon than helium, nitrogen and air gas environment. Pulse energy should exceed energy threshold （about 50 m J） which can generate plasma, and the energy should not exceed about 300 mJ to avoid plasma shielding. Under optimum parameters, concentration relative standard deviation of C, Si, Mn, P, S, Ni, and Cr for low-alloyed steel （sample number 11278） which were measured 11 times is 2.37%, 2.18%, 2.23%, 7.8%, 9.34%, 1.92%, and 2.13%, respectively. And the detection limit of C, Si, Mn, P, S, Ni, and Cr for pure steel is 0.0045%, 0.0072%, 0.0069%, 0.0027%, 0.0024%, 0.0047%, and 0.0024%, respectively.

关键词： laser-induced breakdown spectroscopy (LIBS) analytical performance plasma diagno- sis plasma shielding

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performance verification and comparison of TianLong automatic hypersensitive hepatitis B virus DNA quantification system with Roche CAP/CTM system

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World Journal of Gastroenterology 2017年第37期23卷 6845-6853页

作者： Ming Li Lin Chen Li-Ming Liu Yong-Li Li Bo-An Li Bo Li Yuan-Li Mao Li-Fang Xia Tong Wang Ya-Nan Liu Zheng Li Tong-Sheng Guo School of Mechanical Engineering Xi'an Jiaotong University Xi'an Tian Long Science and Technology Co. Ltd. Center of Clinical Laboratory Medicine 302 Military Hospital of China Department of Laboratory Medicine Beijing Xiaotangshan Hospital

AIM To investigate and compare the analytical and clinical performance of Tian Long automatic hypersensitive hepatitis B virus(HBV) DNA quantification system and Roche CAP/CTM *** Two hundred blood samples for HBV DNA testing, HBV-DNA negative samples and high-titer HBV-DNA mixture samples were collected and prepared. National standard materials for serum HBV and a worldwide HBV DNA panel were employed for performance verification. The analytical performance, such as limit of detection, limit of quantification, accuracy, precision, reproducibility, linearity, genotype coverage and cross-contamination, was determined using the Tian Long automatic hypersensitive HBV DNA quantification system(TL system). Correlation and Bland-Altman plot analyses were carried out to compare the clinical performance of the TL system assay and the CAP/CTM system. RESULTS The detection limit of the TL system was 10 IU/m L, and its limit of quantification was 30 IU/m L. The differences between the expected and tested concentrations of the national standards were less than ± 0.4 Log10 IU/m L, which showed high accuracy of the system. Results of the precision, reproducibility and linearity tests showed that the multiple test coefficient of variation(CV) of the same sample was less than 5% for 102-106 IU/m L; and for 30-108 IU/m L, the linear correlation coefficient r2 = 0.99. The TL system detected HBV DNA(A-H) genotypes and there was no cross-contamination during the "checkerboard" test. When compared with the CAP/CTM assay, the two assays showed 100% consistency in both negative and positive sample results(15 negative samples and 185 positive samples). No statistical differences between the two assays in the HBV DNA quantification values were observed(P > 0.05). Correlation analysis indicated a significant correlation between the two assays, r2 = 0.9774. The Bland-Altman plot analysis showed that 98.9% of the positive data were within the 95% acceptable range, and the maximum di

关键词： analytical performance Hepatitis B virus DNA quantification Clinical performance Hepatitis B virus Real-time quantification PCR

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