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Journal of Environmental Sciences 2023年第5期127卷 133-142页

作者： Jiaqi Fu Bing Yan Shuanglin Gui Yinxuan Fu Song Xia Institute of Energy Research Jiangxi Academy of SciencesNanchang 330096China

To improve methane production from sewage sludge(SS),co-digestion of SS and microalgae(MA)was studied and the application of thermo-alkaline pretreatment to MA was *** results showed that thermo-alkaline pretreatment at 90℃ for 120 min on MA was the optimum pretreatment ***,when the volatile solids(VS)ratio of SS and MA was 1:2,the methane yield reached maximum(368.94 mL/g VS).Fourier transform infrared(FT-IR)and thermogravimetric analysis confirmed the synergetic effects of thermoalkaline pretreated MA on its co-digestion with *** analyses of microbial community indicated that Methanobacterium and Methanosarcina were the dominant methanogens during the co-digestion ***,the relative abundance of Methanosarcina in thermoalkaline pretreated groups was higher compared to unpretreated *** microbial community structure might be affected by thermo-alkaline pretreatment rather than by the MA dosage in the co-digestion.

关键词： anaerobic co-digestion Thermo-alkaline pretreatment Microalgae Sewage sludge Microbial community

Effects of cow manure ratios on methane production and microbial community evolution in anaerobic co-digestion with different crop wastes

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International Journal of Agricultural and Biological Engineering 2022年第5期15卷 219-228页

作者： Jianjun Hao Shufei Jia Hao Sun Gaopan Chen Jiaxing Zhang Yubin Zhao Yang Song Jianguo Zhao Yaya Wang Shuang Liu college of Mechanical and Electrical Engineering Hebei Agricultural UniversityBaoding 071000HebeiChina Key Laboratory of Intelligent Equipment and New Energy Utilization of Livestock and Poultry Breeding Hebei ProvincialBaoding 071001HebeiChina Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation/college of Resources and Environmental Sciences China Agricultural UniversityBeijing 100193China college of Agronomy/Center of Biomass Engineering China Agricultural UniversityBeijing 100193China Key Laboratory of Broiler and Layer Breeding Facilities Engineering Ministry of Agriculture and Rural AffairsBaoding 071001HebeiChina Hebei Animal Husbandry station Shijiazhuang 050035China

The present study investigated the effects of cow manure ratios mixed with maize stover,rice straw,and wheat stalk at 3,2,1(total solid based,TS-based),respectively,on methane production and microbial community structure during the anaerobic co-digestion *** showed cow manure co-digested with maize stover,wheat stalk,and rice straw at ratios of 2,1,and 3 had the highest cumulative methane yields(272.99,153.22167.73 mL/g volatile solid(VS),respectively)and better stability(***,volatile fatty acids(VFAs)and their component).The main microbe evolution had a similar trend which was Petrimonas and Methanosaeta in the early digestion process(Days 0-7)and then evolved into Longilinea,Ruminofilibacter,and Methanosarcina with the progress of digestion,but the relative abundance of these microbes in each reactor was *** was worth noting that Caldicoprobacter in cow manure to maize stover ratio of 2,and to rice straw ratio of three reactors had a relatively higher proportion than reactor of cow manure to wheat stalk ratio of 1,and Hydrogenophaga was the specific bacterium in cow manure to wheat stalk ratio of 1 *** addition,Petrimonas showed positive relationship with VFAs and Longilinea was the *** and Methanobacterium contributed the most during the peak period of methane production in cow manure and maize stover co-digested reactor,and showed positive relationship with acetic ***,Methanosarcina and Methanospirillum made a great contribution during the peak period of methane production in cow manure co-digested with wheat stalk and rice straw *** findings could provide further information on the application of cow manure co-digested with crop wastes.

关键词： cow manure ratio methane production microbial community evolution crop wastes anaerobic co-digestion

anaerobic co-digestion of rice straw and digested swine manure with different total solid concentration for methane production

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International Journal of Agricultural and Biological Engineering 2014年第6期7卷 79-90页

作者： Darwin Jay J.Cheng Zhimin Liu Jorge Gontupil O-Seob Kwon Department of Agricultural Engineering Syiah Kuala UniversityBanda AcehIndonesia Department of Biological and Agricultural Engineering North Carolina State UniversityRaleighNC 27695USA Department of Environmental Science and Engineering Inje UniversityGimhaeGyeongnam 621-749Korea

This study aimed to investigate potential methane production through anaerobic co-digestion of rice straw and digested swine manure with different total *** research was carried out in bench scale with utilizing batch *** evaluate the stability of anaerobic co-digestion process,the experiment was run in *** anaerobic co-digestion process was operated in 500 mL batch digesters under constant agitation speed and *** agitation speed was maintained at 270 r/*** of the batch system was set and maintained at 35℃.Digested swine manure utilized in this experiment was obtained from semi-continuous digesters run at steady state condition,with 25 days of hydraulic retention time under mesophilic *** straw(RS)generated the highest methane production at 3% total solids(TS)which was around(1814±47.43)mL,where in this concentration,it had C:N ratio at 10.6:*** straw obtained the highest methane yield at 3% TS,which was around(141.4±3.70)mL CH_(4)/g volatile solids(VS)*** straw also had the highest chemical oxygen demand(coD)removal and VS reduction at 3% TS which were around(52.97%±1.46%)and(61.81%±1.04%),respectively.

关键词： anaerobic co-digestion rice straw digested swine manure methane production

Effects of mixture ratio on anaerobic co-digestion with fruit and vegetable waste and food waste of China

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Journal of Environmental Sciences 2011年第8期23卷 1403-1408页

作者： Jia Lin Jiane Zuo Lili Gan Peng Li Fenglin Liu Kaijun Wang Lei Chen Hainan Gan State Key Joint Laboratory of Environment Simulation and Pollution control (SKLESPC) School of EnvironmentTsinghua'University Beijing 100084 China. Joint R & D Ceter of Tsinghua Univesity (Department of Environmental Science and Engineering) andShandong Sifon Environmental Protection & Bio-Energy co. Ltd. Beijing 100084 China

The biochemical methane potentials for typical fruit and vegetable waste （FVW） and food waste （FW） from a northern China city were investigated, which were 0.30, 0.56 m3 CH4/kgVS （volatile solids） with biodegradabilities of 59.3% and 83.6%, respectively. Individual anaerobic digestion testes of FVW and FW were conducted at the organic loading rate （OLR） of 3 kg VS/（m3-day） using a lab-scale continuous stirred-tank reactor at 35°C. FVW could be digested stably with the biogas production rate of 2.17 ma/（m3-day） and methane production yield of 0.42 m3 CH4/kg VS. However, anaerobic digestion process for FW was failed due to acids accumulation. The effects of FVW： FW ratio on co-digestion stability and performance were further investigated at the same OLR. At FVW and FW mixing ratios of 2：1 and 1：1, the performance and operation of the digester were maintained stable, with no accumulation of volatile fatty acids （VFA） and ammonia. Changing the feed to a higher FW content in a ratio of FVW to FW 1：2, resulted in an increase in VFAs concentration to 1100-1200 rag/L, and the methanogenesis was slightly inhibited. At the optimum mixture ratio 1：1 for co-digestion of FVW with FW, the methane production yield was 0.49 m3 CH4/kg VS, and the volatile solids and soluble chemical oxygen demand （scoD） removal efficiencies were 74.9% and 96.1%, respectively.

关键词： anaerobic co-digestion biochemical methane potential continuous stirred-tank reactor (CSTR) fruit and vegetable waste food waste

Methanogenic community dynamics in anaerobic co-digestion of fruit and vegetable waste and food waste

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Journal of Environmental Sciences 2012年第7期24卷 1288-1294页

作者： Jia Lin Jiane Zuo Ruofan Ji Xiaojie Chen Fenglin Liu Kaijun Wang Yunfeng Yang State Key Joint Laboratory of Environment Simulation and Pollution control(SKLESPC) School of EnvironmentTsinghua UniversityBeijing 100084China

A lab-scale continuously-stirred tank reactor （CSTR）, used for anaerobic co-digestion of fruit and vegetable waste （FVW） and food waste （FW） at different mixture ratios, was operated for 178 days at the organic loading rate of 3 kg VS （volatile solids）/（***）. The dynamics of the Archaeal community and the correlations between environmental variables and methanogenic community structure were analyzed by polymerase chain reactions - denaturing gradient gel electrophoresis （PCR-DGGE） and redundancy analysis （RDA）, respectively. PCR-DGGE results demonstrated that the mixture ratio of FVW to FW altered the community composition of Archaea. As the FVW]FW ratio increased, Methanoculleus, Methanosaeta and Methanosarcina became the predominant methanogens in the community. Redundancy analysis results indicated that the shift of the methanogenic community was significantly correlated with the composition of acidogenic products and methane production yield. Different mixture ratios of substrates led to different compositions of intermediate metabolites, which may affect the methanogenic community. These results suggested that the analysis of microbial communities could be used to diagnose anaerobic processes.

关键词： high-solid organic waste anaerobic co-digestion methanogenic community structure denaturing gradient gel elec-trophoresis (DGGE) redundancy analysis (RDA)

Improvement of Methane Production from corn Stalk for Whole Slurry anaerobic co-digestion Under Hydrothermal Wastewater Pretreatment

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Journal of Northeast Agricultural University(English Edition) 2023年第1期30卷 44-55页

作者： Sun Yong Wang Ze-hao Qu Jing-bo Cao Guang-li Zheng Guo-xiang Sun Jia-zheng college of Engineering Northeast Agriculture UniversityHarbin 150030China School of Environment Harbin Institute of TechnologyHarbin 150030China college of Business Finance University of AdelaideAdelaide 5000Australia

corn stalk hydrothermal wastewater(CSHW)was used as a pretreatment for whole-slurry anaerobic co-digestion instead of conventional acid *** pretreatment was conducted at 20℃,35℃and 50℃for 3,6 and 9 h,after which all experimental groups were digested at 35℃for 24 *** pretreatment method efficiently broke the lignocellulose structure of the corn *** from the volatile fatty acids(VFAs)content,the pH and RS contents were relatively higher than those of the control during the pretreatment ***,the highest methane production[185.03 mL•g-1 VS(volatile solid)]was achieved at 55.46%under 35℃in 6 h,which was higher than that of the normal corn stalk anaerobic *** VFAs contents and pH increased compared to CK upon the addition of NaOH to adjust pH,and the RS content also increased slightly due to the degradability of lignocellulose during the whole-slurry anaerobic *** work provided a potential method to sustainably treat wastewater and improve fermentation performance.

关键词： pretreatment anaerobic co-digestion corn stalk hydrothermal wastewater corn stalk

Phylogenetic Analysis of anaerobic co-digestion of Animal Manure and corn Stover Reveals Linkages between Bacterial communities and digestion Performance

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Advances in Microbiology 2016年第12期6卷 879-897页

作者： Fan Yang Rui Chen Zhengbo Yue Wei Liao Terence L. Marsh Department of Agricultural and Biosystems Engineering Iowa State University Ames IA USA Department of Biosystems and Agricultural Engineering Michigan State University East Lansing MI USA School of Resources and Environmental Engineering Hefei University of Technology Hefei China Department of Microbiology and Molecular Genetics Michigan State University East Lansing MI USA

Over 3 million tons of manures are produced annually in the United States and pose environmental and health risks if not remediated. anaerobic digestion is an effective method in treating organic wastes to reduce environmental impacts and produce methane as an alternative energy. Previous studies suggested that optimization of feed composition, hydraulic retention time, and other operational conditions can greatly improve total solids removal and increase methane productivity. These environmental factors improve functionality by altering the microbial community structure but explicit details of how the bacterial community shifts are poorly understood. Our investigations were conducted to investigate the relationship between environmental factors, microbial community structure and bioreactor efficiency by using metagenomic analysis of the microbial communities. Our results indicated that the bioreactor with the greatest methane production, digestion efficiency and reduced levels of E. coli/Shigella had a distinctive community structure at the genus level with unique and abundant uncultivated strains of Bacteroidetes. Moreover the same bioreactor was enriched in Aminomonas paucivorans and Clostridia populations that can utilize secondary metabolites produced during cellulose/hemicellulose degradation to generate hydrogen and acetate. Hence specific digestion conditions that enrich for these populations may provide a route to the optimization of co-digestion systems and control the variability in reactor performance.

关键词： anaerobic co-digestion Bacteroidetes Cellulose Degrading Bacterial community

Bioenergy Production from anaerobic co-digestion of Sewage Sludge and Abattoir Wastes

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Advances in Chemical Engineering and Science 2016年第3期6卷 281-287页

作者： Dida Aberra Fekadu Fufa School of Civil and Environmental Engineering Jimma Institute of Technology Jimma University Oromia Ethiopia

Energy is the pillar of human economic development. Several energy sources, renewable and non-renewable, have been exploited to assure and sustain the need for sustainable development. However, depletion of non-renewable energy sources forced researchers to search for alternative cost effective and environmental friendly energy sources. Thus, conversion of waste materials into energy has obtained considerable attention. In line with this, the aim of this study is to investigate the improvement of bio-energy production through anaerobic digestion of mixture of wastes from sewage sludge and abattoir sources. The abattoir waste is functioned as a co-substrate. Laboratory scale batch anaerobic co-digestion of the waste is carried out under mesophilic condition for 20 days. Sewage Sludge (SS) alone, and different mix ratios of SS to Abattoir Waste (AW) were analyzed for bioenergy production. Besides, the nutrient values and reduction in volume of the sewage after digestion were determined. The results show that methane productions of 33.8%, 48.3% and 56.9% were obtained for SS alone and for SS:AW mix ratios of 4:1 and 3:2, respectively. The nutrient values of the slurry increased as mix ratio decreased due to the increase in the amount of AW. The obtained results indicate that bio-energy production can be improved through co-digestion of SS using AW as a co-substrate;thus warranting further investigation for the practical application in the energy production.

关键词： Bio-Energy anaerobic co-digestion Abattoir Wastes Sewage Sludge

comparison of life cycle assessment of large-scale biogas projects with different raw materials in China

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INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING 2024年第2期17卷 268-279页

作者： Sun, Jiazheng Qu, Youpei Lyu, Xiaoyi Ding, Xinjie Miao, Xinying Awasthi, Mukesh Kumar Qu, Jingbo City Univ Hong Kong Sch Energy & Environm Hong Kong 999077 Peoples R China Northeast Agr Univ Coll Engn Harbin 150030 Heilongjiang Peoples R China Key Lab Agr Renewable Resources Utilizat Technol & Harbin 150030 Peoples R China Northwest A&F Univ Coll Nat Resources & Environm Yangling 712100 Shaanxi Peoples R China

The anaerobic digestion (AD) disposal of stover and cattle manure is of great significance to the development of lowcarbon economy and green energy in China, but it will also have an impact on the environment, and the degree of influence is different for various raw materials. In this study, life cycle assessment (LCA) methods were applied to analyze and compare the impact of corn stovers biogas projects (CSBP) and dairy manure biogas projects (DMBP) on the environment during the whole operation stage. The results of inventory analysis were evaluated by ReCiPe2016 Hierarchy(H) mid -point (problem -oriented) and end -point (destruction -oriented) method, respectively. The results showed that the net energy efficiency of CSBP was higher (763.903 kWh/FU) and the greenhouse gas (GHG) emission reduction of DMBP was more (5541.418 kg co2-eq/FU). The anaerobic digestion (AD) units have the greatest environmental impacts, and human carcinogenic toxicity is the largest environmental impact category (1.16-1.43 PE). The key to reducing environmental impact is reducing the input of chemical substances and the waste of electric energy. Both CSBP and DMBP have a favorable impact on ecosystem quality and resources, and CSBP is more beneficial to the environment (-10.297 Pt). co -digestion is an important measure to reduce the environmental damage from biogas projects. These research results provide theoretical support for the selection of raw materials for large-scale biogas projects in China, provide technical basis for reducing the impact of actual operation on the environment, and promote the resource utilization of agricultural waste and carbon dioxide emission reduction and sequestration.

关键词： life cycle assessment environmental impact assessment large-scale biogas projects bioenergy carbon dioxide emission reduction anaerobic co-digestion VOLATILE FATTY-ACIDS ELECTRICITY PRODUCTION ENVIRONMENTAL IMPACTS METHANE PRODUCTION ASSESSMENT LCA RICE STRAW ENERGY USE WASTE SUSTAINABILITY