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Highly Efficient Synthesis of Novel Fluorine Bearing Quinoline-4-carboxylic Acid and the Related Compounds as amylolytic Agents

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International Journal of Organic Chemistry 2012年第1期2卷 49-55页

作者： Mohammed S. T. Makki Dina A. Bakhotmah Reda M. Abdel-Rahman Department of Chemistry Faculty of Science King Abdulaziz University Jeddah Saudi Arabia

Highly efficient syntheses of novel fluorine bearing quinoline-4-carboxylic acids and the related compounds had been achieved from cyclocondensation of 2-amino-5-fluorophenyl glyoxylic acid 1 with benzoyle asetanilides 2 in boiling DMF, to give the target 3. Decarboxylation of 3 produced 6-fluoro-2-phenyl-3-(substituted amino)-keto-quinolines 4, while that reaction underwent refluxing, afforded 7-fluoro-1-(aryl)-3-phenyl-pyrrolo[3,4-c] quinoline-2,9-diones 5. Structure of the products has been established from their elemental and spectral analysis. All targets exhibited a high to moderate activity against some Aspergillus fungi as amylolytic agents.

关键词： amylolytic Activity amylolytic Agents Aspergillus fungi Efficient Synthesis Fluoroquinoline Quinoline-4-carboxylic Acids

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Sweet Potato Starch as a Carbon Source for Growth and Glucoamylase Production from <i>Aspergillus niger</i>

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Advances in Microbiology 2014年第12期4卷 788-795页

作者： Alfred O. Ubalua Tissue Culture Unit Biotechnology Research and Development Center National Root Crops Research Institute (NRCRI) Umuahia Nigeria

A mesophilic strain of Aspergillus niger isolated from cassava effluent samples produced extracellular glucoamylase in submerged culture containing 2% (w/v) soluble or sweet potato starch. On soluble starch medium the maximum glucoamylase activity in the culture filtrate was 9.40 U/mg compared to 8.24 U/mg on sweet potato starch culture filtrate. The mycelial dry weight for both media was 494 and 418 mg respectively. The maximum glucoamylase activity was obtained at a growth temperature of 40°C and pH 4.5. The implication is that the bioprocess of utilizing sweet potato starch in the culture is attractive due to its relatively cheaper availability in Nigeria, making it even more favorable when economics is considered.

关键词： Aspergillus niger Sweet Potato Starch Submerged Fermentation amylolytic Activity Glucoamylase Prodcution

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Inter-Conversion of Carbohydrate Reserves from Pollen Maturation to Rehydration in a Chili Pepper

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American Journal of Plant Sciences 2013年第6期4卷 1181-1186页

作者： Carolina Carrizo García Massimo Guarnieri Ettore Pacini Dipartimento di Scienze Ambientali “G. Sarfatti” Università degli Studi di Siena Siena Italia. Instituto Multidisciplinario de Biología Vegetal (CONICET) Córdoba Argentina

Carbohydrate metabolism is critical for male fertility. Carbohydrate reserves (soluble sugars and starch) were quantified in three advanced stages of pollen development, from the final maturation to rehydration, in a chili pepper cultivar (Capsicum annuum L. cv. Calypso Red) with partially dehydrated pollen, to define possible carbohydrate inter-conversions. At the same time, the activity of soluble enzymes involved in sucrolysis and amylolysis were quantified to reveal potential metabolic pathways. The carbohydrates found (sucrose, glucose, fructose, and starch) apparently inter-convert. There would be a close relation between enzymatic activities and substrates, and the carbohydrate reserves could be key factors on the regulation of enzymatic activities. All the enzymes tested were active, although the relevance of each one changed along pollen maturation and rehydration, defining different profiles of enzymatic activities for each stage. Some usually neglected enzymes (e.g. soluble neutral invertase) have shown an important role in the stages analyzed, suggesting alternative processes to evaluate in the studies of male fertility control.

关键词： Pollen Advanced Development Soluble Carbohydrates Sucrolytic Enzymes amylolytic Enzymes

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