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stocking density affects the growth performance and metabolism of amur sturgeon by regulating expression of genes in the GH/IGF axis

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Journal of Oceanology and Limnology 2018年第3期36卷 956-972页

作者： REN Yuanyuan WEN Haishen LI Yun LI Jifang Key Laboratory of Mariculture（Ocean University of China） Ministry of EducationQingdao 266003China

The effects of stocking density on the growth and metabolism of amur sturgeon were assessed. amur sturgeon were grown for 70 days at three dif ferent stocking densities(low stocking density, LSD: 5.5 kg/m^3; medium stocking density, MSD: 8.0 kg/m^3; and high stocking density, HSD: 11.0 kg/m^3), and the biometric index, muscle composition, and serum biochemical parameters were evaluated. In addition, pituitary, liver, and muscle samples were collected for gene cloning and expression analyses. After 70 days of growth, the fish maintained at HSD had significantly lower fi nal body weight and specifi c growth rate, and a higher feed conversion ratio than those of the fish in the MSD and LSD groups. The HSD group had the lowest lipid and protein concentrations in serum and muscle. The serum cortisol concentration increased significantly in the HSD group, indicating that the stress-response system was activated in these fish. There was no change in the concentration of serum insulin-like growth factor 2(IGF-2), while the concentrations of serum growth hormone(GH) and insulin-like growth factor 1(IGF-1) decreased in the HSD group. The full-length cDNAs of G H and IGF-2 genes(995-bp and 1 207-bp long, respectively), were cloned and analyzed. In the HSD group, the expressions of GH in the pituitary and growth hormone receptor( GHR) and IGF-1 in the liver were down-regulated at the end of the 70-day experiment. In the HSD group, the transcript level of IGF-2 significantly decreased in the liver, but did not change in muscle. Overall, our results indicated that a HSD negatively af fects the growth performance and leads to changes in lipid and protein metabolism in amur sturgeon. The down-regulated expression of genes related to the GH/IGF axis may be responsible for the poor growth performance of amur sturgeon under crowding stress.

关键词： amur sturgeon stocking density growth metabolism growth hormone (GH) insulin-like growth factor(IGF) axis

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