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An ethanolic extract of Bailian(Radix ampelopsis Japonicae):demonstration of colorectal cancer treatment efficacy via inhibition of β-catenin signaling in vitro

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Journal of Traditional Chinese Medicine 2019年第3期39卷 339-345页

作者： Su Tao Wang Xinning Li Chunyu Bai Jingxuan Chi-Yan Cheng Fu Xiuqiong Li Ting Yu Zhiling Consun Chinese Medicines Research Centre for Renal Diseases School of Chinese Medicine Hong Kong Baptist University Kowloon Tong Hong Kong 999077 China HKBU Shenzhen Research Institute and Continuing Education Shenzhen 518001 China Centre for Cancer and Inflammation Research School of Chinese Medicine Hong Kong Baptist University Hong Kong999077 China

OBJECTIVE:To investigate the underlying mechanism of Bailian (Radix ampelopsis Japonicae,BL) extract action on colorectal cancer (CRC).METHODS:We explored the involvement of β-catenin signaling on the anti-CRC effects of an BL ethanolic extract (BLE) in cell models by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,immunofluorescent staining,luciferase assay,Western blot analysis and real-time quantitative polymerase chain reaction ***-CRC compounds were quantified by high performance liquid ***:The contents of gallic acid,catechin,and epicatechin in the BLE were 0.23,1.25,and 0.18 g/kg,***-mediated cytotoxic and apoptotic effects were accompanied by lowered β-catenin/Tcf transcriptional activity,reduced β-catenin nuclear localization,and downregulated protein and mRNA levels of both β-catenin and molecules regulated by β-***:The mechanism underpinning the anti-CRC effects of BLE may involve inhibition of β-catenin *** studies are necessary to establish the role of β-catenin signaling in the action of BLE-mediated anti-CRC effects.

关键词： Bailian (Radix ampelopsis Japonicae) Colorectal neoplasms Beta Catenin Cytotoxins Apoptosis

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Systems pharmacology approach reveals the antiinflammatory effects of ampelopsis grossedentata on dextran sodium sulfate-induced colitis

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World Journal of Gastroenterology 2018年第13期24卷 1398-1409页

作者： You-Lan Chen Ya-Li Zhang Yan-Cheng Dai Zhi-Peng Tang Institute of Digestive Disease Longhua Hospital Shanghai University of Traditional Chinese Medicine Department of Gastroenterology Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

AIM To investigate the protective effects of ampelopsis grossedentata(AMP) on dextran sulfate sodium(DSS)-induced colitis in mice based on systems pharmacology *** Systems pharmacology approach was used to predict the active ingredients, candidate targets and the efficacy of AMP on ulcerative colitis(UC) using a holistic process of active compound screening, target fishing, network construction and analysis. A DSSinduced colitis model in C57 BL/6 mice(n = 10/group) was constructed and treated with 5-aminosalicylic acid(100 mg/kg/d) and AMP(400 mg/kg/d) to confirm the underlying mechanisms and effects of AMP on UC with western blot analyses, polymerase chain reaction, histological staining and *** The therapeutic effects of AMP against DSS-induced colitis were determined in the beginning, and the results showed that AMP significantly improved the disease in general observations and histopathology analysis. Subsequent systems pharmacology predicted 89 corresponding targets for the four candidate compounds of AMP, as well as 123 candidate targets of UC, and protein-protein interaction networks were constructed for the interaction of putative targets of AMP against UC. Enrichment analyses on TNF-α and RANKL/RANK, a receptor activator of NF-κB signaling pathways, were then carried out. Experimental validation revealed that inflammation-related signaling pathways were activated in the DSS group, and AMP significantly suppressed DSS-induced high expression of IRAK1, TRAF6, IκB and NF-κB, and inhibited the elevated expression levels of TNF-α, IL-1β, IL-6 and *** AMP could exert protective effects on UC via suppressing the IRAK1/TRAF6/NF-κB-mediated inflammatory signaling pathways.

关键词： Ulcerative colitis ampelopsis grossedentata Traditional Chinese medicine Systems pharmacology Inflammation

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