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A new compound from the fruit of amorpha fruticosa and activity against acetaminophen-induced hepatotoxicity

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Chinese Chemical Letters 2009年第8期20卷 942-944页

作者： Yun Peng Diao Kun Li Shan Shan Huang Ke Xin Liu Ting Guo Kang College of Pharmacy Dalian Medical University Dalian 116044 China College of Pharmacy Liaoning University of Traditional Chinese Medicine Dalian 116001 China College of Chemistry and Chemical Engineering Liaoning Normal University Dalian 116029. China

A new isoflavone was obstained from the medicinal herb amorpha fruticosa. It was elucidated asbenzopyran- 12-one, 1,4,10,11 - tetrahydro-6′-[8′-（hydroxymethy1）etheny1]-2,3-dimethoxy-8′-O-β-glucopyranosy1-O-α-D-arabinoside by spectroscopic methods including UV, IR, 1D NMR and 2D NMR techniques. And the activity against acetaminophen-induced hepatotoxicity of this compound was also studied, and found this compound can protect liver obviously from hepatotoxicity induced by acetaminophen （AAP）. ？2009 Ting Guo Kang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

关键词： amorpha fruticosa Isoflavone Activity against AAP

Effect of Cryopreservation on Germination of Seeds of amorpha fruticosa and Platycladus orientalis

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Chinese Forestry Science and Technology 2008年第4期7卷 33-38页

作者： HOU Longyu DUAN Xinfang LI Qingmei LIU Guangquan ZHANG Junfeng Institute of Botany Chinese Academy of SciencesBeijing 100093P.R.China Institute of New Forestry Technology Chinese Academy of ForestryBeijing 100091P.R.China Institute of Forestry Chinese Academy of ForestryBeijing 100091P.R.China Northwest Agriculture and Forestry University Yangling 712100P.R.China College of Forestry Beijing Forestry UniversityBeijing 100083P.R.China

In order to explore the feasibility of cryopreservation storage on forest tree seeds, germination of amorpha fruticosa and Platycladus orientalis seeds with moisture content (MC) changing was tested after storage in liquid nitrogen of -196 oC for 30 d. The results showed that both species of seeds can keep high germination percentage after cryopreservation. For A. fruticosa, the germination percentage of seeds was the highest when 4.1% MC was used for storage, and for P. orientalis it was the highest when 6.0% MC was used for storage. It is feasible for both species of seeds to be stored in liquid nitrogen.

关键词： cryopreservation germination seed amorpha fruticosa Platycladus oriental

Cloning a Full-length cDNA Encoding UDP-glucose Pyrophosphorylase from amorpha fruticosa by PCR-based Methods

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High Technology Letters 2002年第3期8卷 5-10页

作者：刘文哲 Hu Xuejun Gao Xiaorong Yuan Xiaodong Liu Zhe FAN Qi An Lijia Department of Bioengineering Dalian University of Technology Dalian 116029 P.R.China Takara Biotechnology Co. Ltd. Dalian 116600 P.R.China

A method based on degenerate Oligo primed polymerase chain reaction (PCR) and random amplification of cDNA end (RACE) PCR for cloning a full length cDNA is described. An amorpha fruticosa cDNA clone encoding UDP glucose pyrophosphorylase (UGP), a key enzyme producing UDP glucose in the synthesis of sucrose and cellulose, is cloned by using this method. We design 5’ RACE primers based on UGPA1 fragment, which obtains from degenerate PCR. Inverse PCR and nested PCR enable cloning of the remainder 5’ and 3’ end fragments of the gene. The deduced amino acid sequence exhibits significant homology with the other UGP genes cloned. This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes.

关键词： gene cloning random amplification of cDNA end PCR UDP glucose pyrophosphorylase amorpha fruticosa

Cloning of A Full-Length cDNA Encoding 4-Coumarate:CoA Ligase of amorpha fruticosa by PCR-Based Methods

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Forestry Studies in China 2002年第1期4卷 13-17页

作者：刘文哲胡学军高晓蓉袁晓东刘哲范琦安利佳大连理工大学生物工程系大连116029 宝生物大连有限公司大连116600

An amorpha fruticosa cDNA encoding 4 coumarate:CoA ligase (4CL), a key enzyme of phenylpropanoid metabolism related to lignin forming, was cloned by degenerating oligo primed polymerase chain reaction (PCR) and rapid amplification of cDNA end (RACE) PCR. We designed 5′RACE primers based on 4CLA1 fragment which obtained from degenerate PCR. Inverse PCR and nested PCR enabled cloning of the remainder fragments of the gene included 5′ and 3′ end sequence. The ORF encodes a polypeptide of 540 amino acids. The predicted amino acid sequence exhibits significant homology with those of other cloned 4CL genes, contain domains typical of predicted 4CL proteins, in particular a postulated AMP binding site, catalytic domain, and conserved Cys residues.

关键词： gene cloning random amplification of cDNA end PCR amorpha fruticosa 4 coumarate:CoA ligase lignin