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Isolation and characterization of facultative mixotrophic ammonia-oxidizing bacteria from constructed wetlands

Journal of Environmental Sciences 2011年第10期23卷 1699-1708页

作者： Soulwène Kouki Neila Saidi Fadhel M'hiri Houda Nasr Hanène Cherif Hadda Ouzari Abdennaceur Hassen Laboratory of Treatment and Water Recycling Centre of Research and Water TechnologiesTechnopark of Borj-CedriaBP 2738020SolimanTunisia Unit of Transfer and Innovation Technologies Tunis International Centre for Environmental TechnologiesBoulevard of the Leader Yasser ArafatLa Charguia1080TunisTunisia Laboratory of Microbiology and Active Biomolecules Department of BiologyFaculty of Sciences of Tunis2092TunisTunisia

Autotrophic ammonia-oxidizing bacteria （AOB） have been widely studied in constructed wetlands systems, while mixotrophic AOB have been less thoroughly examined. Heterotrophic bacteria were isolated from wastewater and rhizospheres of macrophytes of constructed wetlands, and then cultivated in a mixotrophic medium containing ammonium and acetic acid. A molecular characterization was accomplished using ITS-PCR amplification, and phylogenetic analysis based on 16S rRNA gene Sequences. Results showed the presence of 35 bacteria, among 400 initially heterotrophic isolates, that were able to remove ammonia. These 35 isolates were classified into 10 genetically different groups based on ITS pattern. Then, a collection of 10 isolates were selected because of their relatively high ammonia removal efficiencies （ARE≥ 80%） and their phylogenetic diversity. In conditions of mixotrophy, these strains were shown to be able to grow （increase of optical density OD660 during incubation with assimilation of nitrogen into cellular biomass） and to oxidize ammonia （important ammonia oxidation efficiencies, AOE between 79% and 87%）. Among these facultative mixotrophic AOB, four isolates were genetically related to Firmicutes （Bacillus and Exiguobacterium）, three isolates were affiliated to Actinobacteria （Arthrobacter） and three other isolates were associated with Proteobacteria （Pseudomonas, Ochrobactrum and Bordetella）.

关键词： ammonia-oxidizing bacteria mixotroph constructed wetlands ammonia oxidation macrophytes' rhizosphere

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Active ammonia-oxidizing bacteria and archaea in wastewater treatment systems

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Journal of Environmental Sciences 2021年第4期33卷 273-282页

作者： Maosheng Zheng Shishi He Yueqi Feng Mingyuan Wang Yong-Xin Liu Chenyuan Dang Jiawen Wang The Key Laboratory of Resources and Environmental Systems Optimization Ministry of EducationCollege of Environmental Science and EngineeringNorth China Electric Power UniversityBeijing 102206China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China The Key Laboratory of Water and Sediment Sciences Ministry of EducationCollege of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China

ammonia-oxidizing bacteria(AOB)and archaea(AOA)are two microbial groups mediating nitrification,yet little is presently known about their abundances and community structures at the transcriptional level in wastewater treatment systems(WWTSs).This is a significant issue,as the numerical abundance of AOA or AOB at the gene level may not necessarily represent their functional role in ammonia *** amo A genes as molecular markers,this study investigated the transcriptional abundance and community structure of active AOA and AOB in 14 *** PCR results indicated that the transcriptional abundances of AOB amo A(averaged:1.6×10^(8)copies g^(-1)dry sludge)were higher than those of AOA(averaged:3.4×10^(7)copies g^(-1)dry sludge)in all WWTSs despite several higher abundances of AOA amo A at the gene ***,phylogenetic analysis demonstrated that Nitrosomonas europaea and unknown clusters accounted for 37.66%and 49.96%of the total AOB amo A transcripts,respectively,suggesting their dominant role in driving ammonia ***,AOA amo A transcripts were only successfully retrieved from 3 samples,and the Nitrosospaera sister cluster dominated,accounting for 83.46%.Finally,the substrate utilization kinetics of different AOA and AOB species might play a fundamental role in shaping their niche differentiation,community composition,and functional *** study provides a basis for evaluating the relative contributions of ammonia-oxidizing microorganisms(AOMs)to nitrogen conversions in WWTSs.

关键词： ammonia-oxidizing bacteria ammonia-oxidizing archaea Transcriptional activity Community structure Wastewater treatment system

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Community analysis of ammonia-oxidizing bacteria in activated sludge of eight wastewater treatment systems

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Journal of Environmental Sciences 2010年第4期22卷 627-634页

作者： Xiaohui Wang Xianghua Wen Craig Criddle George Wells Jie Zhang Yin Zhao Department of Environmental Science and Engineering Tsinghua University Beijing 100084 China. Department of Civil and Environmental Engineering Jerry Yang & Akiko Yamazaki Environment & Energy Building Stanford University Stanford CA94305-4020 USA Beijing Drainage Group Co. Ltd. Beijing 100084 China

We investigated the communities of ammonia-oxidizing bacteria （AOB） in activated sludge collected from eight wastewater treatment systems using polymerase chain reaction （PCR） followed by terminal restriction fragment length polymorphism （T-RFLP）, cloning, and sequencing of the α-subunit of the ammonia monooxygenase gene （amoA）. The T-RFLP fingerprint analyses showed that different wastewater treatment systems harbored distinct AOB communities. However, there was no remarkable difference among the AOB T- RFLP profiles from different parts of the same system. The T-RFLP fingerprints showed that a full-scale wastewater treatment plant （WWTP） contained a larger number of dominant AOB species than a pilot-scale reactor. The source of influent affected the AOB community, and the WWTPs treating domestic wastewater contained a higher AOB diversity than those receiving mixed domestic and industrial wastewater. However, the AOB community structure was little affected by the treatment process in this study. Phylogenetic analysis of the cloned amoA genes clearly indicated that all the dominant AOB in the systems was closely related to Nitrosomonas spp. not to Nitrosospira spp. Members of the Nitrosomonas oligotropha and Nitrosomonas communis clusters were found in all samples, while members of Nitrosomonas europaea cluster occurred in some systems.

关键词： activated sludge ammonia-oxidizing bacteria T-RFLP amoA gene wastewater treatment plant

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Characterization of bacterial Community,ammonia-oxidizing bacteria,and Nitrospira During the Operation of a Commercial-Scale Recirculating Aquaculture System for Culturing Pufferfish Takifugu rubripes

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Journal of Ocean University of China 2020年第6期19卷 1399-1408页

作者： MA Yuexin YU Zichao DU Xin ZHANG Tao WANG Ning TAO Wei Key Laboratory of Environment Controlled Aquaculture Ministry of EducationDalian 116023China Liaoning Key Laboratory of Marine Animal Immunology and Disease Control Dalian Ocean UniversityDalian 116023China Dalian Tianzheng Industry Company Limited Dalian 116011China College of Environmental Science and Engineering Dalian Maritime UniversityDalian 116026China

We investigated the changes in communities of bacteria,ammonia-oxidizing bacteria,and Nitrospira during the operation of a pufferfish Takifugu rubripes recirculating aquaculture system by using high-throughput DNA *** in bacterial communities were observed at days 1-32,47-62 and 78-93 of biofilm development by using 16S rRNA gene *** relative abundance of Proteobacteria(Gammaproteobacteria)increased,while that of Bacteroidetes(Flavobacteria)*** proportions of Nitrosomonas and Nitrospina ranged from 0.02%to 0.30%and from 0.02%to 0.83%,*** monooxygenase gene pyrosequencing revealed that the top three operational taxonomic units were related to Nitrosomonas aestuarii(17.5%-61.1%),uncultured beta proteobacterium clone B67S-54(1.9%-45.2%),and uncultured bacterium clone AZPa8(3.6%-24.7%).Nitrite oxidoreductase gene pyrosequencing revealed that the relative abundance of the dominant strain Nitrospira *** 2.1 increased,but that of the abundant species Nitrospira marina *** results demonstrated that the communities of bacteria,ammonia-oxidizing bacteria,and Nitrospira were changing during the operation of the pufferfish recirculating aquaculture system.

关键词： biofilter bacterial community ammonia-oxidizing bacteria Nitrospira recirculating aquaculture system

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Community structure of b-Proteobacterial ammonia-oxidizing bacteria in prawn farm sediment

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Progress in Natural Science:Materials International 2008年第6期 679-684页

作者： Ying Maa,Lin Wang a,Lumin Qianb,* aThe Key Laboratory of Science and Technology for Aquaculture and Food Safety,Fisheries College,Jimei University,Xiamen 361021,China bThe Third Institute of Oceanography,SOA,Xiamen 361005,China

To examine the community structure of b-Proteobacterial ammonia-oxidizing bacteria(AOB)in prawn farm sediment,the 16S rRNA gene library was constructed with b-Proteobacterial AOB-specific *** library was screened by PCR-restriction fragment length polymorphism(RFLP)analysis and clones with unique RFLP patterns were *** groups of b-Proteobacterial AOB,the Nitr-osomonas and the Nitrosospira,were *** Nitrosomonas occupied an absolute dominant position,accounting for more than 90% of total clones in the clone library,while the Nitrosospira accounting for 5.48%.Nitrosomonas-aliated clones were grouped into the Nitrosomonas marina and the Nitrosomonas ***143 clusters,and Nitrosospira-aliated clones were grouped into the Nitrosospira cluster *** other clusters of b-Proteobacterial AOB were *** results enriched our knowledge of AOB diversity in the prawn farm sediment and provided important foundational data for further functional studies of these microbes in mariculture environments.

关键词： ammonia-oxidizing bacteria Prawn farm sediment Phylogenetic diversity 16S rRNA gene

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Elevated atmospheric CO2 concentration effects on soil nitrification activity and ammonia-oxidizing bacteria in a Chinese rice-wheat rotation field

Elevated atmospheric CO2 concentration effects on soil nitri...

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第十次全国环境微生物学术研讨会

作者： HU Jun-li LIN Xian-gui CHU Hai-yan YIN Rui ZHU Jian-guo (State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China)(Joint Open Laboratory of Soil and the Environment,Institute of Soil Science and Hongkong Baptist University,Nanjing 210008,China)(Graduate University of Chinese Academy of Sciences,Beijing 100049,China)

A FACE (free-air carbon dioxide enrichment) system in a rice-wheat rotation field, conducted from June,2001 at Wuxi,China,was used to investigate the effects of elevated atmospheric CO

关键词： elevated atmospheric CO2 concentration soil ammonia-oxidizing bacteria nitrification activity N application rate

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Effect of Vallisneria natans on community structure and activity of ammonia-oxidizing archaea and bacteria in eutrophic freshwater sediment

Effect of Vallisneria natans on community structure and acti...

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江苏省遗传学会第九次会员代表大会暨学术研讨会

作者： Dongmei Yue Lu Liu Xiaolin Wang Lijuan Jiang Lin Xiao State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University

Nitrification mediated by ammonia-oxidizing bacteria（AOB） and ammonia-oxidizing archaea（AOA） is important in nitrogen transformation. The influence of Vallisneria natans（V. natans） on the abundance and community composition of AOB and AOA in sediments was examined by quantitative PCR and clone library analyses based on the ammonia monooxygenase α-subunit（amoA） gene. The results showed that AOA predominated among ammonia-oxidizing microbes in the sediments of Lake Taihu. Cloning and sequencing of amoA gene showed that the AOB community consisted of Nitrosomonas and unclassified Nitrosomonas-like lineage, and most AOA amoA sequences clustered with environmental clones previously obtained from eutrophic lake and estuarine sediments. Neither AOA nor AOB communities clustered according to vegetation or sediment type. The results of the present study demonstrated that aquatic macrophytes may stimulate the growth and metabolic activity of AOA and/or AOB and subsequently help to the ecological restoration process.

关键词： Vallisneria natans ammonia-oxidizing archaea ammonia-oxidizing bacteria sediment PNR

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Effects of herbicide butachlor application on the growth of periphytic biofilms and nitrogen loss in paddy systems

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Pedosphere 2024年第1期34卷 211-221页

作者： Yanhui ZHAO Mingjun LEI Liandong JING Fan XIA Meixia YAN Jiantong LIU Yonghong WU Yunli WU Chenxi WU Ecology and Environment Monitoring and Scientific Research Center Yangtze Basin Ecology and Environment AdministrationMinistry of Ecological and EnvironmentWuhan 430010China College of Chemistry and Environmental Protection Engineering Southwest Minzu UniversityChengdu 610041China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil SciencesChinese Academy of SciencesNanjing 210008China

The application of butachlor as an herbicide in paddy fields is widely practiced,aiming to increase rice yield by directly or indirectly influencing the paddy *** biofilms,which form at the soil-water interface in paddy fields,are complex bioaggregates that play an important role in nitrogen (N) *** objective of this study was to investigate the effect of butachlor on periphytic biofilm growth and N cycling under both light and dark conditions in the *** results revealed that butachlor application hindered the growth of periphytic biofilms and led to the dominance of Cyanobacteria as the primary prokaryotes,while inhibiting the development of eukaryotic ***,the application of butachlor reduced the richness and diversity of prokaryotes,but increased those of eukaryotes in periphytic *** light treatments exhibited higher total N loss because light favored periphytic biofilm growth and enhanced ammonium (NH_(4)^(+)) assimilation and ***,butachlor application resulted in the increased retention of NH_(4)^(+)-N and nitrate (NO_(3)^(-))-N and an increase in N loss via *** abundances of functional genes encoding enzymes such as ammonia monooxygenase,nitrite reductase,and nitrous oxide reductase were increased by butachlor application,favoring nitrification and denitrification ***,the results suggest that butachlor application leads to an increase in total N loss mainly through denitrification in paddy systems,particularly in the presence of periphytic ***,the results may provide valuable insights into the changes in periphytic biofilm growth and N cycling induced by butachlor,and future studies can further explore the potential implications of these changes in paddy soils.

关键词： ammonia-oxidizing bacteria amoA denitrification denitrifying bacteria nirK nitrification nitrogen cycling nosZ volatilization

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Responses of nitrogen cycling and related microorganisms to brackish wetlands formed by evapotranspiration

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Pedosphere 2024年第1期34卷 252-266页

作者： Jiaohui FANG Tianshu LU Jian LIU Shangbin HE Xiufeng YANG Huashan DOU Honghai ZHANG School of Life Sciences Qufu Normal UniversityQufu 273100China Environment Research Institute Shandong UniversityQingdao 266237China Hulunbuir Academy of Inland Lakes in Northern Cold&Arid Areas Hulunbuir 021000China

Elevated evapotranspiration due to warmer air temperature could raise salinity and nutrient levels of some inland wetlands, potentially impacting nitrogen cycling. To characterize the impact of high evapotranspiration on soil microbial nitrogen cycling in inland wetlands, we compared freshwater and brackish marsh(or non-marsh) wetlands in terms of sediment ammonia-oxidizing rate(AOR), denitrifying rate(DR), and related microbial communities in a typical inland basin, the Hulun Lake basin, in China. Results showed that marsh ecosystems(ME) exhibited 31% higher AOR and 65% higher DR than non-marsh ecosystems(NE). For NE, freshwater non-marsh wetland exhibited 12% higher AOR than brackish non-marsh wetland. This was probably due to the inhibitory effects of high NH_4~+ and salinity levels on ammonia-oxidizing archaea in brackish non-marsh wetland. Conversely, DR in brackish non-marsh wetland was 23% higher than that in freshwater non-marsh wetland, with total organic carbon(TOC) significantly influencing this difference,suggesting that the higher DR in brackish non-marsh wetland was mainly due to its higher TOC level. For ME, due to the direct and indirect interference of salinity, brackish marsh wetland displayed 26% lower AOR and 19% lower DR than freshwater marsh wetland. Besides, brackish wetlands harbored distinct ammonia-oxidizing and denitrifying microbial communities compared to freshwater wetlands. The assembly of these communities was dominated by stochastic processes, while brackish wetlands exhibited more prominent deterministic processes than freshwater wetlands. Overall, high evapotranspiration altered activities and community characteristics of ammonia oxidizers and denitrifiers in inland brackish wetlands by enhancing salinity and nutrient levels,while emergent plants occurring in ME could mitigate the adverse effects of salt stress of inland brackish wetlands on nitrogen cycling.

关键词： ammonia-oxidizing archaea ammonia-oxidizing bacteria aquatic plants denitrifying bacteria nitrogen transformation inland wetlands

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Variation of Potential Nitrification and ammonia-oxidizing bacterial Community with Plant-Growing Period in Apple Orchard Soil

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Journal of Integrative Agriculture 2014年第2期13卷 415-425页

作者： LIU Ling-zhi QIN Si-jun Lü De-guo WANG Bing-ying YANG Ze-yuan Key Laboratory for Northern Fruit Cultivation and Physio-Ecology of Shenyang City/College of Horticulture Shenyang Agricultural University College of Land and Environment Shenyang Agricultural University Foreign Language Department Shenyang Agricultural University

In this study, we investigated the potential nitrification and community structure of soil-based ammonia-oxidizing bacteria （AOB） in apple orchard soil during different growth periods and explored the effects of environmental factors on nitrification activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis （DGGE）. We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen （N） content, NH4＋-N content, NO3-N content, and pH showed significant correlations with AOB abundance and nitrification activity in soil. The Shannon-Winner diversity, as well as species richness and evenness indices （determined by PCR-DGGE banding patterns） in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE fingerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the 13-Proteobacteria phylum, with the dominant AOB showing high similarity to the Nitrosospira genus. Therefore, soil-based environmental factors, such as pH variation and content of NHa＋-N and NO3--N, can substantially influence the abundance of AOB communities in soil, and play a critical role in soil-based nitrification kinetics.

关键词： apple orchard soil ammonia-oxidizing bacteria potential nitrification community structure PCR-dena~'ing gradientgel electrophoresis

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