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Investigation on the Mechanism of Exacerbation of Myasthenia Gravis by aminoglycoside antibiotics in Mouse Model

Journal of Huazhong University of Science and Technology(Medical Sciences) 2005年第3期25卷 294-296页

作者：刘昌勤胡芳 Department of Neurology Union Hospital Tongji Medical College Huazhong University of Science and Technology

To investigate the underlying mechanism of the exacerbation of myasthenia gravis by aminoglycoside antibiotics. C57/BL6 mice were immunized with acetylcholine receptor (AChR), extracted from electric organ of Narcine timilei according to Xu Haopeng's methods, in complete Fruend's adjuvant (CFA) to establish experimental autoimmune myasthenia gravis (EAMG). EAMG mice were divided randomly into 5 groups: MG group, NS group and three antibiotics groups. The clinical symptom scores of mice were evaluated on d7 after the last immunization and d14 of antibiotics treatment. Repetitive nerve stimulation (RNS) was performed and the levels of anti-AChR antibody (AChR-Ab) were tested at the same time. The mean clinical symptom grades of gentamycin group (1.312, 2.067), amikacin group (1.111, 1.889) and etimicin group (1.263, 1.632) were significantly higher than those of MG group (1.000, 1.200) (Pantibiotics groups were 69.23 %, 58.82 % and 63.16 % respectively, which were significantly higher than those of MG group and NS group (40.00 %, 40.00 %, Pantibiotics groups were also higher than those of MG group. Our results indicated that aminoglycoside antibiotics could aggravate the symptom of myasthenia gravis. The exacerbation of myasthenia gravis by these antibiotics probably involves competitively restraining the release of acetylcholine from presynaptic membrane, impairing the depolarization of postsynaptic membrane, depressing the irritability of myocyte membrane around the end-plate membrane and consequently leading to the blockade of neuromuscular junction.

关键词： aminoglycoside antibiotics myasthenia gravis mice

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Resonance Light Scattering Method for Determination of Amikacin with Potassium Ferrioxalate as a Probe

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Chemical Research in Chinese Universities 2010年第3期26卷 366-370页

作者： HU Xiao-li AN Lan-xiang LIU Shao-pu LIU Zhong-fang LI Cui-xia Key Laboratory of Luminescence and Real-Time Analysis Ministry of Education School of Chemistry and Chemical Engineering Southwest University Chongqing 400715 P. R. China

In a weak acid medium, potassium ferrioxalate（PF） can react with some aminoglycoside（AGs） antibiotics, such as amikacin（AMK）, kanamycin（KANA）, tobramycin（TOB） and gentamicin（GEN）, to form ion-association complexes. It results in the enhancement of resonance light scattering（RLS） in different degrees. The maximum scattering peaks are all located at 345 nm. Among them, the relative scattering intensity（AIRLs） of AMK system is much higher than that of KANA, TOB or GEN. Therefore the method is more propitious to the determination of trace amounts of AMK. The optimum reaction conditions, influencing factors, and the relationship between scattering intensity and concentration of antibiotics were investigated by means of the proposed method. The enhancement of RLS signals is directly proportional to the concentration of antibiotics in a certain range of concentration. A new resonance light scattering method for the determination of AMK and other aminoglycoside antibiotics with [Fe（C2O4）3]^3- as a probe is thus established based on it. The method exhibits high sensitivity and good selectivity. The detection limit（3σ） for AMK is 1.8 ng/mL. The method can be applied to the determination of AMK in clinical serum samples. The reaction mechanism and the reasons for RLS enhancement are discussed in this paper.

关键词： Resonance light scattering Amikacin aminoglycoside antibiotics Potassium ferrioxalate

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aminoglycoside Hydrogels

Aminoglycoside Hydrogels

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2018（第3届）抗菌科学与技术论坛

作者：程义云胡婧婧 School of Life Sciences East China Normal University

aminoglycosides are a family of critically important antibiotics for the treatment of serious infections including multidrug-resistant pathogens. However, clinical use of aminoglycoside antibiotics is compromised by bacterial biofilm formation at subinhibitory concentrations or adverse side effects such as otoxicity and nephrotoxicity at high antibiotic doses. Preparation of aminoglycoside formulation that allows on-demand drug delivery is a solution to this sticky issue. Here, we designed a family of aminoglycoside hydrogels by cross-linking oxidized polysaccharides such as dextran, carboxyl methyl cellulose, alginate, and chondroitin using aminoglycosides as cross-linkers. The hydrogel modulus, degradation rate and release kinetics can be precisely modulated by tailoring the aminoglycoside dose during gel formation. The aminoglycoside hydrogel showed fast gelation, self-healing and on-demand drug release behaviors, and high antibacterial activities in vitro and in vivo against both Gram-positive and Gram-negative bacteria. The talk will present a facile and promising strategy to develop smart hydrogels for tropical administration of aminoglycoside antibiotics.

关键词： Antibacterial hydrogels aminoglycoside antibiotics on-demand delivery biofilm all-small-molecule dynamic covalent hydrogels

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Spectrum and frequency of mitochondrial 12S rRNA variants in the Chinese subjects with nonsynrdomic hearing loss in Zhejiang Province

Spectrum and frequency of mitochondrial 12S rRNA variants in...

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第十二次全国医学遗传学学术会议

作者： Zheng Binjiao Peng Guanghua Chen Bobei Fang Fang Zheng Jing Wu Yue Liang Lingzhi Nan Benyu Tang Xiaowen Zhu Yi Lu Jianxin Guan Minxin College of Life Sciences Zhejiang University

Objective mitochondrial DNA(mtDNA) mutation are one of the important causes of *** particular,the 12S rRNA gene is the hot spots for mutations associated with both aminoglycoside ototoxicity and nondyndromic *** in this report,a total of 318 Chinese pediatric hearing-impaired subjects were recruited from otology clinics in the Zhejiang Province,*** these subjects underwent clinical,genetic evaluation and molecular analysis of 12S *** analysis identified 34 variants in the 12S rRNA gene in this *** incidences of the known deafness-associated 1555A>G,1494C>T and 1095T>C mutations were 9.1%,0.6%and 1.25%in this cohort,*** mtDNA variants were evaluated by structural and phylogenetic *** these,the 839A>G and 1452T>C variants could confer increased sensitivity to aminoglycosides or nonsyndromic deafness as they were not present in 449 Chinese controls and localized at highly conserved nucleotides of the 12S rRNA. However,other variants appeared to be *** these data further support the idea that mitochondrial 12S rRNA is one of major targets for aminoglycoside *** data have been providing valuable information to predict which individuals are at risk for ototoxicity,to improve the safety of aminoglycoside antibiotic therapy,and eventually to decrease the incidence of deafness.

关键词： mitochondrial 12S rRNA gene non-syndromic deafness aminoglycoside antibiotics mutation spectrum hearing loss

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