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Healing property of the Piper betel phenol, allylpyrocatechol against indomethacin-induced stomach ulceration and mechanism of action

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World Journal of Gastroenterology 2007年第27期13卷 3705-3713页

作者： S Bhattacharya D Banerjee AK Bauri S Chattopadhyay SK Bandyopadhyay Department of Biochemistry Dr. B.C. Roy Post Graduate Institute of Basic Medical Sciences IPGME&R 244B Acharya Jagadish Chandra Bose Road Kolkata-700020 India Bio-Organic Division Bhabha Atomic Research Centre Mumbai-400085 India

AIM：To evaluate the protective activity of allylpyrocatechol （APC）, the major antioxidant constituent of Piper betel, against the indomethacin-induced stomach ulceration in the rat model and correlates with its antioxidative and mucin protecting properties.METHODS： Male Sprague-Dawley rats were divided into five groups. Normal control rats （group Ⅰ ） were given the vehicle oral dose of gum acacia in distilled water （1 mL per rat）; ulcerated control and treated rats （groups Ⅱ-V） were given a single dose of indomethacin （30 mg/kg body wt.）; group Ⅱ rats were sacrificed 4 h after indomethacin administration; groups Ⅲ-Ⅴ rats were given the vehicle （1 mL per rat） or APC （2 mg/kg body wt.） or misoprostol （1.43 μg/kg body wt.） once daily by oral intubation for 7 d starting from 4 h after the indomethacin administration. After 7 d, the stomach tissues were excised for histological examination and biochemical analysis. RESULTS： Treatment with APC （2 mg/kg body wt per day） and misoprostol （1.43 μg/kg body wt per day） for 7 d could effectively heal the stomach ulceration as revealed from the ulcer index and histopathological studies. Compared to the zero day ulcerated group, treatment with APC and misoprostol reduced the ulcer index by 93.4% and 85.4% respectively （P 〈 0.05）. Both APC and misoprostol accelerated ulcer healing observed in natural recovery （P 〈 0.05）, their respective healing capacities not being significantly different. The healing capacities of APC and misoprostol could be attributed to their antioxidant activity as well as the ability to enhance the rnucin content of the gastric tissues. Compared to the ulcerated untreated rats, those treated with APC and rnisoprostol showed near normal MDA levels, while the protein levels were 86% and 78% of the normal value respectively （P 〈 0.05）. Likewise, both APC and rnisoprostol increased the SOD, catalase, and rnucin levels significantly （P 〈 0.05）, the effect of APC being better. CONCLUSION： APC can protect ind

关键词： allylpyrocatechol Antioxidant Histopathology Indornethacin Mucin Piper betel Stomach ulcer

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Killing of Staphylococcus aureus by allylpyrocatechol is potentiated by induction of intracellular oxidative stress and inhibition of catalase activity

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Journal of Integrative Medicine 2016年第6期14卷 456-464页

作者： Roslinah Mohamad Hussain Noor Faradilla Abdullah Zulkhairi Amom Department of Medical Laboratory Technology Faculty of Health SciencesUniversiti Teknologi MARA Department of Anatomy and Basic Sciences Faculty of Health SciencesUniversiti Teknologi MARA

OBJECTIVE： This study investigated the effects of allylpyrocatechol （APC）, the major component in ethanolic extract of Piper betle, on key oxidative stress resistance enzymes important for the survival of Staphylococcus aureus, a major pathogen in the human host. METHODS： Effects of APC on expressions of genes encoding catalase （katA）, superoxide dismutases （SODs）, including soda and sodM, and alkyl hydroperoxide reductase （ahpC） in S. aureus were quantitated by RT-qPCR in reference to gyrA and 16S rRNA. Corresponding activities of the enzymes were also investigated. The Livak analysis was performed for verification of gene-fold expression data. Effects of APC on intracellular and extracellular reactive oxygen species （ROS） levels were determined using the nitroblue tetrazolium （NBT） reduction assay. RESULTS： APC-treated S. aureus cells had higher sodA and sodM transcripts at 1.5-fold and 0.7-fold expressions respectively with corresponding increase in total SOD activity of 12.24 U/mL compared to untreated cells, 10.85 U/mL （P〈0.05）. Expression of ahpC was highest in APC-treated cells with 5.5-fold increased expression compared to untreated cells （P〈0.05）. Correspondingly, ahpC activity was higher in APC-treated cells at 0.672 （A310nm） compared to untreated cells which was 0.394 （A310nm）. In contrast, katA expression was 1.48-fold and 0.33-fold lower respectively relative to gyrA and 16S rRNA. Further, APC-treated cells showed decreased catalase activity of 1.8 × 10^-4 （U/L or μmol/（min.L）） compared to untreated cells, which was 4.8 × 10^-4 U/L （P〈0.05）. Absorbance readings （A575nm） for the NBT reduction assay were 0.709 and 0.695 respectively for untreated and treated cells, which indicated the presence of ROS. APC-treated S. aureus cells had lower ROS levels both extracellularly and intracellularly, but larger amounts remained intracellularly compared to extracellular levels with absorbances of 0.457 and 0.137 respectively （P〈0.05）. CONCLUSION： APC induced express

关键词： allylpyrocatechol catalase superoxide dismutase alkyl hydroperoxide reductase reactiveoxygen species Staphylococcus aureus Piper beUe

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