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Serum Helicobacter pylori Kat A and Ahp C antibodies as novel biomarkers for gastric cancer

World Journal of Gastroenterology 2016年第21期22卷 5060-5067页

作者： Bing Zhang Hai-Lin Li Qing Fan Fang Guo Xi-Yun Ren Hai-Bo Zhou Ji-Wei Zhu Ya-Shuang Zhao Wen-Jing Tian Department of Epidemiology College of Public Health Harbin Medical University Harbin 150081 Heilongjiang Province China Xiangfang Center for Disease Control and Prevention Harbin 150081 Heilongjiang Province China Department of Forensic Medicine Harbin Medical University Harbin 150081 Heilongjiang Province China

AIM: To investigate catalase (KatA) and alkyl hydroperoxide reductase (AhpC) antibodies of Helicobacter pylori as biomarkers for gastric cancer (GC). METHODS: This study included 232 cases and 264 controls. Recombinant KatA and AhpC proteins were constructed and the levels of antibodies were tested by indirect enzyme-linked immunosorbent assay (ELISA). Logistic regression was applied to analyze the relationships between KatA, AhpC and GC. The chi(2) trend test was used to evaluate the dose-response relationships between serum KatA and AhpC antibody levels and GC. Receiver operating characteristic (ROC) curve was used to evaluate the screening accuracy of KatA and AhpC as biomarkers. Combined analysis was used to observe screening accuracy of predictors for GC. RESULTS: In all subjects, the association between KatA and AhpC and GC risk was significant (P < 0.001) with odds ratio (OR) = 12.84 (95%CI: 7.79-21.15) and OR = 2.4 (95%CI: 1.55-3.73), respectively. KatA and AhpC antibody levels were strongly related to GC risk with a dose-dependent effect (P for trend < 0.001). The area under the ROC (AUC) for KatA was 0.806, providing a sensitivity of 66.81% and specificity of 86.36%; and the AUC for AhpC was 0.615, with a sensitivity of 75.65% and specificity of 45.49%. The AUC was 0.906 for KatA and flagella protein A (FlaA) combined analysis. CONCLUSION: Serum KatA and AhpC antibodies are associated with GC risk and KatA may serve as a biomarker for GC. KatA/FlaA combined analysis improved screening accuracy.

关键词： Helicobacter pylori Catalase Serum antibody alkyl hydroperoxide reductase Gastric cancer Case-control study

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Killing of Staphylococcus aureus by allylpyrocatechol is potentiated by induction of intracellular oxidative stress and inhibition of catalase activity

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Journal of Integrative Medicine 2016年第6期14卷 456-464页

作者： Roslinah Mohamad Hussain Noor Faradilla Abdullah Zulkhairi Amom Department of Medical Laboratory Technology Faculty of Health SciencesUniversiti Teknologi MARA Department of Anatomy and Basic Sciences Faculty of Health SciencesUniversiti Teknologi MARA

OBJECTIVE： This study investigated the effects of allylpyrocatechol （APC）, the major component in ethanolic extract of Piper betle, on key oxidative stress resistance enzymes important for the survival of Staphylococcus aureus, a major pathogen in the human host. METHODS： Effects of APC on expressions of genes encoding catalase （katA）, superoxide dismutases （SODs）, including soda and sodM, and alkyl hydroperoxide reductase （ahpC） in S. aureus were quantitated by RT-qPCR in reference to gyrA and 16S rRNA. Corresponding activities of the enzymes were also investigated. The Livak analysis was performed for verification of gene-fold expression data. Effects of APC on intracellular and extracellular reactive oxygen species （ROS） levels were determined using the nitroblue tetrazolium （NBT） reduction assay. RESULTS： APC-treated S. aureus cells had higher sodA and sodM transcripts at 1.5-fold and 0.7-fold expressions respectively with corresponding increase in total SOD activity of 12.24 U/mL compared to untreated cells, 10.85 U/mL （P〈0.05）. Expression of ahpC was highest in APC-treated cells with 5.5-fold increased expression compared to untreated cells （P〈0.05）. Correspondingly, ahpC activity was higher in APC-treated cells at 0.672 （A310nm） compared to untreated cells which was 0.394 （A310nm）. In contrast, katA expression was 1.48-fold and 0.33-fold lower respectively relative to gyrA and 16S rRNA. Further, APC-treated cells showed decreased catalase activity of 1.8 × 10^-4 （U/L or μmol/（min.L）） compared to untreated cells, which was 4.8 × 10^-4 U/L （P〈0.05）. Absorbance readings （A575nm） for the NBT reduction assay were 0.709 and 0.695 respectively for untreated and treated cells, which indicated the presence of ROS. APC-treated S. aureus cells had lower ROS levels both extracellularly and intracellularly, but larger amounts remained intracellularly compared to extracellular levels with absorbances of 0.457 and 0.137 respectively （P〈0.05）. CONCLUSION： APC induced express

关键词： allylpyrocatechol catalase superoxide dismutase alkyl hydroperoxide reductase reactiveoxygen species Staphylococcus aureus Piper beUe

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