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Journal of Ocean University of China 2019年第3期18卷 710-718页

作者： LIU Yuan SUI Zhenghong ZHANG Shu MA Jinhua ZHONG Jie Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China

Based on the transcriptomic sequencing, three genes of alexandrium pacificum encoding ubiquitin(UBI), telomerase(TEL) and glycine-rich protein(GRP) relating to cell division were isolated and characterized. The full-length cD NA of GRP was obtained through approach of rapid amplification of c DNA ends(RACE). Four conserved domains, including DNA-and RNAbinding sites or motifs, cold shock domain at the N-terminal, and zinc-finger structure of CCCH type at the C-terminal were idenrtified. Phylogenetic analysis revealed that the deduced amino acid sequence of GRP tends to cluster together with proteins harboring cold shock domain. The expressions of these three genes were analyzed with quantitative PCR(qPCR). It was found that the expressions of these three genes at the logarithmic growth phase and induced logarithmic growth phase were all higher than those at lagging growth phase(P < 0.05). The expression patterns of these three genes were coincident with the proliferative capacity of the algae, e.g., displaying increased expression level at log and induced growth phases. The functions of these genes and their possible roles during harmful algal blooms(HAB) were discussed.

关键词： alexandrium pacificum glycine-rich protein harmful algal bloom qPCR telomerase ubiquitin

High-frequency acoustic backscattering characteristics for acoustic detection of the red tide species Akashiwo sanguinea and alexandrium affine

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Journal of Oceanology and Limnology 2019年第4期37卷 1268-1276页

作者： KIM Hansoo KANG Donhyug JUNG Seung Won KIM Mira Maritime Security and Safety Research Center Korea Institute of Ocean Science & Technology (KIOST) Busan 49111 Korea Department of Ocean System Engineering Jeju National University Jeju 63243 Korea Library of Marine Samples Korea Institute of Ocean Science & Technology (KIOST) Geoje 53201 Korea

Harmful algal blooms (HABs), caused by the overgrowth of certain phytoplankton species, have negative effects on marine environments and coastal fisheries. In addition to cell-counting methods using phytoplankton nets, a hydroacoustic technique based on acoustic backscattering has been proposed for the detection of phytoplankton blooms. However, little is known of the acoustic properties of HAB species. In this study, as essential data to support this technique, we measured the acoustic properties of two HAB species, Akashiwo sanguinea and alexandrium affine, which occur in the South Sea off the coast of Korea. Due to the small size of the target, we used ultrasound for the measurements. Experiments were conducted under laboratory and field conditions. In the laboratory experiment, the acoustic signal received from each species was directly proportional to the cell abundance. We derived a relationship between the cell abundance and acoustic signal received for each species. The measured signals were compared to predictions of a fluid sphere scattering model. When A. sanguinea blooms appeared at an abundance greater than 3 500 cells/mL, the acoustic signals varied with cell abundance, showing a good correlation. These results confirm that acoustic measurements can be used to detect HAB species.

关键词： acoustic backscattering Akashiwo sanguinea alexandrium affine fluid-sphere scattering model harmful algal blooms (HABs) red tide

Preliminary Identification of Three New Isolates in Genus alexandrium(Dinophyceae) from China Sea Area

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Journal of Ocean University of China 2007年第1期6卷 33-39页

作者： FAN Lijing SUI Zhenghong MAO Yunxiang GUO Hao Division of Life Science and Technology Ocean University of ChinaQingdao 266003P.R.China National Marine Environmental Monitoring Center State Oceanic AdministrationDalian 116023P.R.China

The 5.8 S ribosomal DNA sequences (5.8S rDNA) and their flanking regions, internal transcribed spacer 1 and spacer 2 (ITS1 and ITS2) of three new isolates in genus alexandrium (alexandrium sp. qd1, alexandrium sp. qd2, alexandrium sp. gz) from China were amplified, sequenced, and subjected to phylogenetic analysis. alexandrium sp. gz and alexandrium sp. qd1 were grouped with high bootstrap values with four strains/species, i.e., A. catenella South Korea strain, A. catenella Japan strain, and two from China, alexandrium sp. AC03 and alexandrium sp. AN01 being proposed to be A. catenalla in a previous study. Then alexandrium sp. gz and alexandrium sp. qd1 were identified as alexandrium catenella. As A. catenella was isolated from Qingdao and Guangzhou sea areas, it supposedly distributed at least in these two areas and was genetically different. alexandrium sp. qd2 differed greatly from species in alexandrium. It clustered with Symbiodinium californium, Symbiodinium sp. G15 and Gymnodinium sp. Zhao 01 with 100% bootstrap value; so alexandrium sp. qd2 affiliates to genus Symbiodinium, and is probably a free-living Symbiodinium species.

关键词： alexandrium Gymnodinium Symbiodinium ITS 5.8S rDNA-ITS phylogeny

Biodiversity of the Symbiotic Bacteria Associated with Toxic Marine Dinoflagellate alexandrium tamarense

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Journal of Biosciences and Medicines 2015年第6期3卷 23-28页

作者： Xiaoling Zhang Xiaoqing Tian Liyan Ma Bing Feng Qiaohong Liu Lidong Yuan Chengqi Fan Hongliang Huang Hongliang Huang Qiao Yang Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization Ministry of Agriculture East China Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences Shanghai China SOA Key Laboratory for Polar Science Polar Research Institute of China Shanghai China

Paralytic shellfish poisoning (PSP) toxins are potent neurotoxins mainly produced by dinoflagellates and being concentrated in bivalves through food web transfer. Increasing number of findings of toxin-producing bacteria in the cells of dinoflagellate such as alexandriumtamarense supports the hypothesis of the bacterial origin of PSP toxins. Evidence that there are specific symbiosis bacterial taxa associated with the phytoplankton indicates the presence of specific selective mechanisms between them, and implies that the symbiosis bacteria have some vital function to the benefit of the dinoflagellates. Studies on the role of toxin-producing symbiosis bacteria in the marine ecosystem are considered to be becoming more important. Although toxigenic bacteria could be isolated from toxic dinoflagellates, it was not clearly proven whether the isolated bacterial strains based on culture-dependent manner and the corresponding intracellular bacteria were the same because of microbial unculturability. This paper aims to demonstrate the biodiversity of the symbiotic bacteria associated with toxic dinoflagellate A. tamarense using the culture-indepen- dent high-throughput pyrosequencing method, as well as the phylogenetic analysis based on 16S rDNA sequences of the symbiotic cultivable bacteria strains isolated from toxic Alexander tamarense.

关键词： Symbiotic Bacteria alexandrium tamarense Paralytic Shellfish Poisoning Biodiversity

甲藻分类历史沿革及中国近海部分甲藻分类地位修订

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生物多样性 2013年第1期21卷 19-27页

作者：马新李瑞香李艳潘玉龙国家海洋局第一海洋研究所青岛266061 海洋生态环境科学与工程国家海洋局重点实验室青岛266061 国家海洋局北海分局青岛266033

甲藻自发现至今已有200多年的历史。一方面,甲藻的分类地位越来越受到学者的重视;另一方面,随着研究的深入,出现了越来越多的争议和困惑,在一定程度上阻碍了甲藻分类学的发展。本文简述了国内外甲藻分类研究的历史沿革,主要介绍了几次... 详细信息

甲藻自发现至今已有200多年的历史。一方面,甲藻的分类地位越来越受到学者的重视;另一方面,随着研究的深入,出现了越来越多的争议和困惑,在一定程度上阻碍了甲藻分类学的发展。本文简述了国内外甲藻分类研究的历史沿革,主要介绍了几次较大的分类地位的变革。另外,目前中国近海甲藻的分类体系与国际上通用的分类体系还有很多差异,为了更好地促进国内甲藻的分类学研究,方便国际间的合作与交流,我们对国际国内的甲藻分类体系(分别以algaeBASE数据库和《中国海洋生物名录》为代表)进行比较,发现目水平上的分类体系基本相同,但在科、属的划分上有很多差异。针对这些差异点查阅文献,寻根溯源,最终提出了一个较为合理的划分。本文所作的主要调整有:将凯伦藻属(Karenia)、卡罗藻属(Karlodinium)和塔卡藻属(Takayama)3个属从裸甲藻科(Gymnodiniaceae)中分出,单独成凯伦藻科(Kareniaceae);角藻属(Ceratium)中的大部分海洋种改为新角藻属(Neoceratium);亚历山大藻属(alexandrium)从屋甲藻科(Goniodomataceae)中分出,归入膝沟藻科(Gonyaulacaceae);取消异沟藻科(Heteraulacus),由屋甲藻科取而代之;成立2个新目,即尖尾藻目(Oxyrrhinales)和梨甲藻目(Pyrocystales)。

关键词：甲藻分类 Neoceratium alexandrium Kareniaceae Oxyrrhinales Pyrocystales