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The effect of inactivation of Aldehyde dehydrogenase on pheromone production by a gut bacterium of an invasive bark beetle,Dendroctonus valens

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Insect Science 2023年第2期30卷 459-472页

作者： Qingjie Cao Tuuli-Marjaana Koski Huiping Li Chi Zhang Jianghua Sun College of Forestry Hebei Agricultural UniversityBaodingHebei ProvinceChina College of Life Science Institute of Life Science and Green DevelopmentHebei UniversityBaodingHebei ProvinceChina State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina Rural Energy and Environment Agency Ministry of AgricultureandRural AffairsBeijingChina

Semiochemical-based management strategies are important for controlling bark beetles,such as invasive Red Turpentine Beetle(Denroctonus valens),the causal agent for mass mortality of pine trees(Pinus spp.)in China.It has been previously shown that the pheromone verbenone regulates the attack density of this beetle in a dose-dependent manner and that the gut bacteria of D.valens are involved in verbenone production.However,molecular functional verification of the role of gut bacteria in the pheromone production of D.valens is still lacking.To better understand the molecular function of gut bacterial verbenone production,we chose a facultative anaerobic gut bacterium(Enterobacter xiangfangensis)of D.valens based on its strong ability to convert cis-verbenol to verbenone,as shown in our previous study,and investigated its transcriptomics in the presence or absence of cis-verbenol under anaerobic conditions(simulating the anoxic environment in the beetle's gut).Based on this transcriptome analysis,Aldehyde dehydrogenase(ALDH1)was identified as a putative key gene responsible for verbenone production and was knocked-down by homologous recombination to obtain a mutant E.xiangfangensis strain.Our results show that these mutants had significantly decreased the ability to convert the monoterpene precursor to verbenone compared with the wild-type bacteria,indicating that ALDH1 is primarily responsible for verbenone conversion for this bacterium species.These findings provide further mechanistic evidence of bacterially mediated pheromone production by D.valens,add new perspective for functional studies of gut bacteria in general,and may aid the development of new gene silencing-based pest management strategies.

关键词： Aldehyde dehydrogenase Dendroctonus valens gut bacteria mutants transcriptome verbenone

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Interferon-gamma inhibits Aldehyde dehydrogenase^(bright)cancer stem cells in the 4T1 mouse model of breast cancer

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Chinese Medical Journal 2022年第2期135卷 194-204页

作者： Xiufen Zhuang Guilan Shi Xiao Hu Huiru Wang Wen Sun Yanhong Wu Department of Oncology Affiliated Hospital of Jiangsu UniversityZhenjiangJiangsu 212000China Department of Immunology Zibo Vocational Institute Health SchoolZiboShandong 255000China Department of Medical Engineering Morsani College of MedicineTampaFL 33612USA Department of Oncology Suqian First HospitalSuqianJiangsu 223800China Department of Blood Transfusion The First Affiliated Hospital of USTCHefeiAnhui 230001China Department of Medical Microbiology and Immunology School of Basic Medical SciencesWannan Medical CollegeWuhuAnhui 241002China.

Background:Despite improvements in disease diagnosis,treatment,and prognosis,breast cancer is still a leading cause of cancer death for women.Compelling evidence suggests that targeting cancer stem cells(CSCs)have a crucial impact on overcoming the current shortcomings of chemotherapy and radiotherapy.In the present study,we aimed to study the effects of T cells and a critical anti-tumor cytokine,interferon-gamma(IFN-γ),on breast cancer stem cells.Methods:BALB/c mice and BALB/c nude mice were subcutaneously injected with 4T1 tumor cells.Tumor growth and pulmonary metastasis were assessed.ALDEFLOUR™assays were performed to identify Aldehyde dehydrogenase^(bright)(ALDH^(br))tumor cells.ALDH^(br)cells as well as T cells from tumor-bearing BALB/c mice were analyzed using flow cytometry.The effects of CD8^(+)T cells on ALDH^(br)tumor cells were assessed in vitro and in vivo.The expression profiles of ALDH^(br)and ALDH^(dim)4T1 tumor cells were determined.The levels of plasma IFN-γwere measured by enzyme-linked immunosorbent assay,and their associations with the percentages of ALDH^(br)tumor cells were evaluated.The effects of IFN-γon ALDH expression and the malignancy of 4T1 tumor cells were analyzed in vitro.Results:There were fewer metastatic nodules in tumor-bearing BALB/c mice than those in tumor-bearing BALB/c nude mice(25.40 vs.54.67,PAldehyde dehydrogenase 1 family member A1 in 4T1 tumor cells(0

关键词： Cancer stem cells Aldehyde dehydrogenase Interferon-gamma CD8+T cells

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Structural Insight into the Substrate Gating Mechanism by Staphylococcus aureus Aldehyde dehydrogenase

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CCS Chemistry 2020年第5期2卷 946-954页

作者： Xuan Tao Zhemin Zhang Xiao Zhang Hongyan Li Hongzhe Sun Zong-Wan Mao Wei Xia MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou510275 Department of Chemistry The University of Hong KongPokfulam RoadHong Kong

Staphylococcus aureus produces staphyloxanthin,a C30 carotenoid with golden color,as an antioxidant to promote bacterial resistance to reactive oxygen species.The biosynthesis pathway of staphyloxanthin involves a series of catalytic enzymes.Aldehyde dehydrogenase(AldH)is a dehydrogenase recently identified to convert 4,4’-diaponeurosporenaldehyde into 4,4’-diaponeurosporenoic acid during staphyloxanthin biosynthesis.Here,we present the crystallographic structures of apo-and holo-forms of S.aureus AldH.The dimeric enzyme contains a unique C-terminal helix,which resembles a“gatekeeper”helix found in human membrane-bound fatty Aldehyde dehydrogenase(FALDH).Particularly,the helix adopts“open”and“closed”conformations in apo-and holo-AldH,respectively,to control the access of the substrate tunnel.Mutagenesis in combination with in vitro and in vivo activity assays identifies several residues essential for S.aureus AldH substrate recognition and enzyme catalytic turnover.Our results provide insights into substrate recognition of S.aureus AldH toward polyunsaturated long-chain aldehydes at atomic resolution.

关键词： Staphylococcus aureus virulence factor substrate recognition Aldehyde dehydrogenase protein structure

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Functional Characterization of an Aldehyde dehydrogenase Homologue in Rice

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Journal of Integrative Agriculture 2012年第9期11卷 1434-1444页

作者： YANG Sheng-hui NIU Xiang-li LUO Di CHEN Chang-dong YU Xu TANG Wei LU Bao-rong LIU Yong-sheng Key Laboratory for Bio-Resource and Eco-Environment Minstry of Education~College of Life Sciences Sichuan University Chengdu 610064 P.R.Chtina College of Life Sciences Chongqing University Chongqing 400044 P.R.China Key Laboratory for Biodiversity Science and Ecological Engineering Ministry of Education~Institute of Biodiversity Science Fudan University Shanghai 200433 P.R.China

The Aldehyde dehydrogenase （ALDH） superfamily of NAD（P）-dependent enzymes, in general, oxidize a wide range of endogenous and exogenous aliphatic and aromatic aldehydes to their corresponding carboxylic acids and play an essential role in detoxification of reactive oxygen species （ROS） accumulated under the stressed conditions. In order to identify genes required for the stresses responses in the grass crop Oryza sativa, a homologue of ALDH gene （OsALDH22） was isolated and characterized. OsALDH22 is conserved in eukaryotes, shares high homology with the orthologs from Aldehyde dehydrogenase subfamily ALDH22. The OsALDH22 encodes a protein of 597 amino acids that in plants exhibit high identity with the orthologs from Zea mays, Sorghum bicolor, Hordeum vulgare and Arabidopsis thaliana, respectively, and the conserved amino acid characteristics for ALDHs are present, including the possible NAD~ binding site （F-V-G-S- P-G-V-G）, the catalytic site （V-T-L-E-L-G-G-K） and the Cys active site. Semi-quantitative PCR and real-time PCR analysis indicates that OsALDH22 is expressed differentially in different tissues. Various elevated levels of OsALDH22 expression have been detected when the seedlings exposed to abiotic stresses including dehydration, high salinity and abscisic acid （ABA）. Transgenic rice plants overexpressing OsALDH22 show elevated stresses tolerance. On the contrary, down- 0 regulation of OsALDH22 in the RNA interference （RNAi） repression transgenic lines manifests declined stresses tolerance.

关键词： rice （Oryza sativa） Aldehyde dehydrogenase abiotic stresses stress tolerance

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Association of alcohol dehydrogenase and Aldehyde dehydrogenase 2 genetic polymorphisms with serum lipid profile: meta-analysis of mendelian randomisation studies

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South China Journal of Cardiology 2014年第4期15卷 238-250,256页

作者： GAO Jia-jia JIN Dong-mei WEN Zhu-zhi IP Chi-kin WANG Jing-feng GENG Deng-feng Department of Cardiology Sun Yat-sen Memorial Hospital Sun Yat-sen University Department of Rehabilitation Medicine Sun Yat-sen Memorial Hospital Sun Yat-sen University

Background Alcohol dehydrogenase （ADH） and Aldehyde dehydrogenase 2 （ALDH2） are the key en- zymes for alcohol metabolism. Several genetic studies have investigated the association between ADH and ALDH2 genetic polymorphisms and serum lipid profile （SLP）, however, the results were inconsistent. Methods Fourteen articles involving 27,917 participants were included in this meta-analysis. Weighted mean difference （WMD） and 95% confidence intervals （CIs） were presented using random effects model. Publication bias was evaluated by funnel plot and Begg＇s test. In addition, to further explore the heterogeneity, subgroup analysis were performed. Results Overall, there was no association between ADH genetic polymorphisms and SLP with no regard for drinking status. However, compared with ALDH2 wild homozygous genotype, ALDH2 mutant genotypes were associated with significant decrease in serum high density lipoprotein cholesterol （HDL- C）（WMD -1.80 mg/dL, 95% CI -1.88 to -1.72, P 〈 0.001） and total cholesterol （TC） levels （WMD -1.]0 mg/dL, 95% CI -1.59 to -0.62, P 〈 0.001）, and significant increase in serum low density lipoprotein choles- terol （LDL-C） level （WMD 0.30 mg/dL, 95% CI 0.18 to 0.43, P 〈 0.001）. Although there was no significant difference in serum triglyceride level in the overall population, subgroup analysis revealed that compared with ALDH2 ＊ 1 wild homozygote, ALDH2 ＊ 2 allele displayed a significant difference in serum triglyceride level between the female and male （female： WMD 1.69 mg/dl, 95% CI 1.08 to 2.30, P 〈 0.001; male： WMD -6.42 mg/dL, 95% CI -12.15 to -0.68, P = 0.028）. Conclusion ADH genetic polymorphism has no association with SLP, regardless of sex category and drinking status. ALDH2 genetic polymorphism has slight association with HDL-C, LDL-C and TC levels and sex-specific association with serum triglyceride level. Whether or not the association between ADH2 genetic polymorphisms and SLP is resulted from alcohol con-sumption needs further investigation.

关键词： alcohol dehydrogenase Aldehyde dehydrogenase serum lipids meta-analysis

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遗传改造大肠杆菌合成吲哚乙酸（英文）

遗传改造大肠杆菌合成吲哚乙酸（英文）

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中国生物工程学会第十三届学术年会暨2019年全国生物技...

作者：郭道义孔思佳潘虹赣南师范大学生命科学学院

Indole-3-acetic acid(IAA) is considered the most common and important naturally occurring auxin in plants and a major regulator of plant growth and development. In this study, an Aldehyde dehydrogenase AldH from Escherichia coli was found to convert indole-3-acetylaldehyde into IAA. Then, we established an artificial pathway in engineered Escherichia coli for microbial production of IAA from glucose. The overall pathway includes the upstream pathway from glucose to L-tryptophan and the downstream pathway from L-tryptophan to IAA. To our knowledge, this is the first report on the biosynthesis of IAA directly from a renewable carbon source. This study described here shows the way for the development microorganism for biosynthesis of auxin and promoting plant growth in the future.

关键词： metabolic engineering Escherichia coli Aldehyde dehydrogenase indole-3-acetic acid

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THE POSSIBLE GENETIC BACKGROUND OF ALCOHOL-INDUCED PANCREATIC STEATOSIS

THE POSSIBLE GENETIC BACKGROUND OF ALCOHOL-INDUCED PANCREATI...

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第十七届西南地区消化病学术会议暨2014贵州省消化病及...

作者： Jing Li Yi Xie Fang Yuan Bin Song Chengwei Tang Department of Gastroenterology West China HospitalSichuan University Division of Peptides Related with Human Diseases Key Laboratory of Biotherapy of Human DiseasesMinistry of Education Department of Radiology West China HospitalSichuan University

Background and objective:AIcohol-induced pancreatic steatosis is associated with alcohol metabolism in the pancreas,in which alcohol dehydrogenase(ADH)and Aldehyde dehydrogenase(ALDH)are the major oxidative enzyme.And genetic polymorphism of ADH2 and ALDH2 is known to be closely related to alcohol dependence.But it is still unknown whether genetic polymorphism of them is also relevant to pancreatic steatosis in alcoholics.This study is to find out the possible genetic background of alcohol-induced pancreatic steatosis in alcoholics by analyzing genetic polymorphism in ADH2 and ALDH2.Methods:163 alcoholic male aged 20-70 years with a normal body mass index were recruited into this study.The alcoholics were defined as the drinkers with an alcohol intake of>80 g/day,a duration of>5 years,and abstinence from alcohol within 2 years.They received magnetic resonance scanning in the epigastric region by using double-echo chemical shift magnetic resonance technique.PCR-restriction fragment length polymorphism was used for ADH2 and ALDH2 genotype detection.The drinkers with pancreatic fat content higher than 4.2%,8.0%in alcoholics younger and older than 50 years respectively were diagnosed as alcohol-induced pancreatic steatosis.ResuIts:The distribution of the different ADH2 and ALDH2 genotypes among the 163 alocholics closely conformed to expected Hardy-Weinberg frequencies(p>0.05).In drinkers,compared with ADH2*2/*2 carriers,ADH2*1/*1 carriers showed a significantly elevated risk of developing pancreatic steatosis(50 years,OR=5.34).No association was found between ALDH2 genotypes and risk of pancreatic steatosis.Conclusion.In drinkers,ADH2*1/*1 carriers had a significantly higher risk to developalcohol-induced pancreatic steatosis.ADH2*l/*lgenotype may be related to alcohol-induced pancreatic steatosis.

关键词： alcohol pancreas steatosis double-echo chemical shift magnetic resonance imaging alcohol dehydrogenase Aldehyde dehydrogenase

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Rapid simultaneous genotyping of polymorphisms in ADHIB and ALDH2 using high resolution melting assay

Rapid simultaneous genotyping of polymorphisms in ADHIB and ...

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第十二次全国医学遗传学学术会议

作者： Xiao-Wen Yuan Wan-Jun Zhou Xuan Shang Liang Li Yan-Hui Liu Xiang-Min Xu Department of Medical Genetics School of Basic Medical SciencesSouthern Medical University

Background:Alcohol dehydrogenase-1B(ADH1B) and Aldehyde dehydrogenase-2 (ALDH2) are important enzymes in human alcohol metabolism.The genetic polymorphisms of ADH1B 143G>A(ADH1B*1/ADH1B*2) and ALDH2 15100>A(ALDH2*1/ALDH2*2) are responsible for serious alcohol related disorders.A rapid,accurate,sensitive and high-throughput assay for genotyping the two genes was developed,and the assay could be useful in the epidemiological studies and risk evaluation of alcohol-related diseases.Methods:In this study,we designed two amplicons with different melting temperature by adding GC-or AT-rich 5’ tails,and the two amplicons include ADH1B 143G>A and ALDH2 1510G>A respectively.A total of 200 genomic DNA samples from Chinese population were enrolled to evaluate the specificity of the assay.40 samples randomly selected from each of the genotype groups tested by HRM assay were validated by DNA sequencing.The assay reproducibility and detection limit was evaluated by replicate experiments and two-fold serial dilutions of DNA concentration,respectively.Results: All samples were unambiguously distinguished by HRM assay.There was 100%concordance between the results of the HRM assay and DNA sequencing.Meanwhile,the assay has highly reproducibility,and the coefficients of variation were in the range 0.022%to 0.062%.In addition, only 6.25ng of genomic DNA can be detected as well.Conclusion:The method we present is proved to be high-throughput and reliable,which is suitable for rapid simultaneous genotyping of all nine genotype groups of ADH1B and ALDH2 gene in a single closed tube with $0.5 in 2 hours.

关键词： alcohol dehydrogenase Aldehyde dehydrogenase genotyping high resolution melting assay

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Expression and regulation of Aldehyde dehydrogenases in prostate cancer

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Journal of Cancer Metastasis and Treatment 2018年第1期4卷 515-531页

作者： Ali I.M.Ibrahim Maria Sadiq Fiona M.Frame Norman J.Maitland Klaus Pors Institute of Cancer Therapeutics School of Pharmacy and Medical SciencesFaculty of Life SciencesUniversity of BradfordWest Yorkshire BD71DPUK Department of Pharmacy Al-Zaytoonah University of JordanAmman 11733Jordan Cancer Research Unit Department of BiologyUniversity of YorkHeslingtonNorth Yorkshire YO105DDUK

The functional role of Aldehyde dehydrogenases(ALDHs)in prostate cancer remains an area of some controversy.Many studies have used high ALDH functional activity to isolate putative cancer stem cells with tumour-initiating and propagating properties,while evidence is also emerging about the involvement of specific isoforms in migration,invasiveness and metastasis.Identification of specific ALDH isoforms,which contribute to both drug resistance and aggressiveness of the disease remains a challenge within the complex heterogeneity of prostate cancer.The purpose of this perspective is to dissect functional roles for ALDH in the tumour microenvironment and to evaluate the potential of the ALDH gene family as biomarkers and/or targets for therapeutic intervention.

关键词： Aldehyde dehydrogenase retinoic acid prostate cancer castration-resistant prostate cancer cancer stem cells multidrug resistance

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Deterioration in Hemodynamics Reaction, Baroreflex Sensitivity, Sympathetic Nerve Activity and Redox State of Thoracic Aorta in the Experimental Model of Nitrate Tolerance and Its Pharmacological Correction

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Pharmacology & Pharmacy 2016年第1期7卷 81-88页

作者： Nikoloz V. Gongadze Tamara D. Kezeli Galina V. Sukoyan Zaza Chapichadze Nino M. Dolidze Makrine Mirziashvili Mariam Chipashvili Department of Medical Pharmacology and Pharmacotherapy Tbilisi State Medical University Tbilisi Georgia Department of Pharmacology Faculty of Medicine I. Javakhishvili Tbilisi State University Tbilisi Georgia International Scientific Centre of Introduction of New Biomedical Technology Tbilisi Georgia Department of Pharmacology State Regulation Agency for Medical Activities Ministry of Health and Social Affairs of Georgia Tbilisi Georgia

Continuous treatment with organic nitrates causes nitrate tolerance and provides evidence for a relationship between mitochondrial complex 1 activity and mitochondrial Aldehyde dehydrogenase-2 (ALDH-2) with disturbances of the hemodynamics reaction during nitroglycerin (NTG) tolerance (NTGT). The purpose of this study was the evaluation of efficacy of original oxidized form NAD-containing drug, NADCIN®, on hemodynamic reactions, baroreflex sensitivity (BRS) and reflex control of splanchnic sympathetic nerve activity (SSNA), level of redox-potential, activity of ALDH-2 and superoxide anion generation in aortic tissue in rat model of NTGT. Five groups (7 - 9 each) of male Wistar rats, including control, acute i.v. NTG (150 mcg/kg) administration, NTG tolerance NTGT treatment with NADCIN® 8 mg/kg and methylene blue (MB, 2.5 mg/kg) were used. NTGT in rats was accompanied with the greatly attenuation of hemodynamics reaction, BRS, the decreasing of the ability to reflex control of SSNA without pronounce overexpression of endothelin-1 in vessels (aorta). In NTGT rats i.v. NTG along induced less hypotensive reactions and alterations in heart period vs single NTG treated group, more expressively decreased BRS (-34%) and reflex control of SSNA (-18%). NADCIN® significantly inhibits tolerance-inducing properties of the prolonged nitroglycerin infusion (max decrease of blood pressure response to nitroglycerin injection, % of normal controls: NTGT 51.2%, NADCIN® 91.6%, MB 55.8%). NADCIN® in NTGT rats after NTG i.v. administration increased reduced BRS (+37.8%, p ® of NTGT rats restores hemodynamics changes, BRS and SSNA throughout the increasing of redox-potential NAD/NADH and cessates the NTGT developing.

关键词： Experimental Model of Nitroglycerin Tolerance Baroreflex Sensitivity Aldehyde dehydrogenase Redox-Potential Splanchnic Sympathetic Nerve Activity

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