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Antidiabetic potential of methanol extracts from leaves of Piper umbellatum L. and Persea americana Mill.

Asian Pacific Journal of Tropical Biomedicine 2018年第3期8卷 160-165页

作者： Guy Sedar Singor Njateng Sumera Zaib Larissa Yetenge Chimi Cesaire Feudjio Raymond Simplice Mouokeu Donatien Gatsing Jules-Roger Kuiate Ezekiel Adewole Jamshed Iqbal Laboratory of Microbiology and Antimicrobial Substances Faculty of Science University of Dschang Centre for Advanced Drug Research COMSATS Institute of Information Technology Institute of Fisheries and Aquatic Sciences University of Douala Department of Chemical Sciences Afe Babalola University Ado

Objective: To determine inhibitory activity of methanolic leaf extract of Piper umbellatum and Persea americana(P. americana)(traditionally used in Cameroon against diabetes) on β-glucosidase, β-glucosidase, maltase-glucoamylase, aldose reductase and aldehyde reductase activities, enzymes involved in starch digestion or diabetic complications. Methods: The methanol extracts from Piper umbellatum and P. americana were prepared by maceration. To assess relative efficacy of these extracts, the determination of concentrations that were needed to inhibit 50% of enzyme activity was done, whereas, gas chromatography-mass spectrum was used to identify components from extracts that may be responsible for the activities. Results:The tested extracts strongly inhibited β-glucosidase, maltase-glucoamylase, aldose reductase and aldehyde reductase activities with IC50 ranging from(1.07 ± 0.03) to(31.77 ± 1.17) μg/*** the tested extracts, P. americana was the most active against sensitive enzymes(IC50 of1.07 ± 0.03 to 15.63 ± 1.23). But, none of the extracts showed interesting inhibitory effect against β-glucosidase as their percentage inhibitions were less than 16%. From gas chromatographymass spectrum analysis, 10 and 8 compounds were identified in Piper umbellatum and *** extracts respectively, using NIST library 2014. Conclusions: Results of this study provide the scientific credential for a prospective usage of these plants to treat diabetes.

关键词： Piper umbellatum Persea americana Glucosidase Maltase-glucoamylase Aldose reductase aldehyde reductase

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Polymorphisms and functions of the aldose reductase gene 5' regulatory region in Chinese patients with type 2 diabetes mellitus

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Chinese Medical Journal 2002年第2期115卷 209-213页

作者：李清解谢平黄建军谷亚鹏曾卫民宋惠萍中南大学湘雅医学院生物化学教研室，长沙，410078

OBJECTIVE: To screen the 5' regulatory region of the aldose reductase (AR) gene for genetic variabilities causing changes in protein expression and affecting the promoter function. METHODS: The screenings were carried out by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). All SSCP variants were submitted for DNA sequencing and inserted into the plasmid chloromycetin acetyl transferase (CAT) enhancer vector. The constructs were used to transfect Hela cells, and CAT assays were performed to assess promoter activity. Gel mobility shift and footprinting assays were also performed to determine the interaction between the DNA and nuclear proteins. RESULTS: Two polymorphisms, C (-106) T and C (-12) G, were identified in the regulatory region in 123 Chinese control subjects and 145 patients with type 2 diabetes mellitus. The frequencies of genotypes WT/WT, WT/C (-12) G and WT/C (-106) T were not significantly different between the subjects and patients. In the patients with and without retinopathy, frequencies of WT/C (-106) T were 31.5% and 17.5% (P 0.05) respectively. The total frequency of WT/C (-12) G and WT/C (-106) T in patients with retinopathy was 41.8%, significantly higher than that (20.0%) in patients without retinopathy (P

关键词： 5' Flanking Region Adult aldehyde reductase Binding Sites China Chloramphenicol O-Acetyltransferase DNA DNA Footprinting Diabetes Mellitus, Type 2 Electrophoretic Mobility Shift Assay Female Hela Cells Humans Male Middle Aged Mutation Polymerase Chain Reaction Polymorphism, Genetic Polymorphism, Single-Stranded Conformational Recombinant Fusion Proteins Regulatory Sequences, Nucleic Acid Research Support, Non-U.S. Gov't Sequence Analysis, DNA Transcription, Genetic

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