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Extract from Zanthoxylum piperitum Induces Apoptosis of AGS Gastric Cancer Cells through akt/mdm2/p53 signaling pathway

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Chinese Journal of Integrative Medicine 2021年第10期27卷 752-759页

作者： Ye Seul park Gun He Nam Kyung Jo Jo Hye Won Kawk Sang Yung Kim Young Min Kim Department of Biological science and Biotechnology College of Life science and Nano TechnologyHannam University1646 Yuseong-daeroYuseong-guDaejeon 34054South Korea Department of Food Science&Bio Technology Shinansan UniversityDeahakro Danwon-guAnsan-CityGyenggi-doKorea

Objective:To determine the effect of Zanthoxylum piperitum extracet(ZpE)on apoptosis and analyze anticancer substances in ZpE,changes in proteins related to apoptosis,and pathological changes in tumors in ***:Fifteen 4-week-old female BALB/c nu/nu mice were divided into 3 groups depending on ZpE dose,with 5 in each *** gastric carcinoma cells(1 x 10^(6) cells/200 jxL)were subcutaneously injected into the flank of each *** week after the injection of AGS cells,ZpE was administered to the skin tissue[10 or 50 mg/(kg-d)]in the low-and high-dose groups,respectively for 20 *** animals were injected with vehicle *** 3 weeks,the tumor was extracted and carried out for immunohistochemistry,the tendency of apoptosis and p53 in the body was checked using TdT-mediated dUTp nick-end labeling(TUNEL)*** 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay,annexin V dead cell staining,cell cycle arrest and Western blotting,AGS gastric carcinoma cells were incubated with various concentrations of ZpE for 24 *** survival rates were analyzed by MTT *** was analyzed using annexin V dead cell staining and cell cycle arrest and measured using Muse cell ***:High performance liquid chromatography(HpLC)analysis showed that ZpE contained organic sulfur compounds such as alliin and *** assay results revealed that ZpE(10-85»xg/mL)could effectively inhibit the growth of AGS gastric cancer cells at higher concentrations(pppendent increase in the apoptosis rate and G!phase in ZpE(10-70 jig/mL)*** decreased the expression of anti-apoptotic proteins(p-akt,p-mdm2,Bcl-2),while increased pro-apoptotic proteins(cleaved pARp,p53,pro-Caspase 3,Bax).TUNEL assays revealed an increase in cell *** staining confirmed the involvement of ***:ZpE decreases AGS cell

关键词： AGS gastric cancer cells Zanthoxylum piperitum apoptosis akt/mdm2/p53 signaling pathway active compounds

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