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Effect of protease inhibitor from agaricus bisporus on glucose uptake and oxidative stress in 3T3-L1 adipocytes

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Asian Pacific Journal of Tropical Biomedicine 2020年第3期10卷 136-146页

作者： Reena Vishvakarma Abha Mishra School of Biochemical Engineering Indian Institute of Technology(Banaras Hindu University)VaranasiUttar Pradesh-221005India

Objective:To explore the effect of the protease inhibitor from agaricus bisporus(J.E.Lange)Imbach(AbPI)on glucose uptake and oxidative stress in 3 T3-L1 adipocytes.Methods:Adipocytes were differentiated and stained with OilRed-O staining to confirm adipogenesis.The toxic/protective effect of AbPI on the adipocytes was determined by MTT assay,intracellular reactive oxygen species generation through flow cytometry,and morphologically through confocal microscopy using propidium iodide,4,6-diamino-2-phenylindol dihydrochloride,and 2’,7’-dichlorofluorescein diacetate dyes.The uptake of fluorescent glucose analog,2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose by adipocytes was also studied through confocal microscopy.Results:MTT assay showed that the cell survival rate was(28.00±3.00)%,(92.33±2.60)%,and(71.34±2.10)%in the presence of 2 mM H2O2,AbPI alone,and AbPI and H2O2 both,respectively,in comparison to the control.Oil-Red-O staining indicated that Ab PI enhanced adipogenesis.AbPI stimulated the glucose uptake by adipocytes similar to the drug rosiglitazone,and showed insulinsensitizing effect in the presence of insulin,but failed to stimulate the uptake in the absence of insulin.Intracellular reactive oxygen species generation was reduced in differentiating adipocytes upon Ab PI treatment.Confocal microscopy showed that the damaged cell population rose to 3.50%,117.84%,and 261.50%in the presence of Ab PI alone,AbPI with H2O2,and H2O2 alone,respectively.Conclusions:The protease inhibitor enhances glucose uptake by adipocytes and exhibits a cytoprotective effect on them.

关键词： Protease inhibitor agaricus bisporus 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose Oxidative stress Hydrogen peroxide 3T3-L1 adipocytes

Effect of Hot Water Extract from agaricus Blazei Murill on Chemotaxis of Neutrophils

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Journal of Cosmetics, Dermatological Sciences and Applications 2013年第1期3卷 12-17页

作者： Mayuko Miyagawa Yuriko Hirono Ayaka Kawazoe Eri Shigeyoshi Masahito Nose Masaaki Sakura K. E. Pinkerton Minoru Takeuchi Center for Health and the Environment University of California Davis USA. Department of Animal Medical Science Faculty of Life Sciences Kyoto Sangyo University Kyoto Japan

Hot water extract from the edible Brazilian mushroom, agaricus Blazei Murill (ABM), is used for both traditional and alternative medicine. ABM is reported to stimulate anti-tumor, anti-infection, and immune activity. However, there are few reports of how ABM affects neutrophils. Therefore, in this study, we examined the effect of hot water ABM extract on neutrophil migration, phagocytosis, and reactive oxygen species production using neutrophils from guinea pig. Migratory direction and velocity as indicators of chemotactic activity of neutrophils were significantly (p 0.001) increased at concentration of 50 and 100 mg/ml in ABM extract compared with control. Phagocytic activity of neutrophil was significantly (p 0.01) increased at concentration of 5 mg/ml in ABM extract compared with control. Production of reactive oxygen species (ROS: H2O2 or ) by neutrophils was significantly (p 0.01) increased at concentration of 5 mg/ml in ABM extract compared with control. These results suggest that enhancement in neutrophil chemotactic activity, phagocytic activity and ROS production are mechanisms by which ABM extract inhibits bacterial infection in the skin and dermatitis.

关键词： Neutrophil Chemotactic Activity Phagocytic Activity ROS Production agaricus Blazei Murill

Effects of Arterially Infused Hydroalcoholic agaricus blazeiExtracts on Perfusion Pressure and Oxygen Uptake in the Bivascularly Perfused Rat Liver

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Journal of Biosciences and Medicines 2015年第10期3卷 74-79页

作者： Andrea Luiza de Oliveira Valoto Jorgete Constantin Fabrício Bracht Rosane Marina Peralta Adelar Bracht Faculdade Integrado de Campo Mourao Campo Mourao Brazil Department of Biochemistry Universidade Estadual de Maringá (UEM) Maringá Brazil Universidade Técnica Federal do Paraná (UTFPR) Campo Mourao Brazil

In a preceding work we have reported experiments showing that an hydroalcoholic exctract of agaricus blazei is able to exert purinergic effects in the isolated perfused rat liver when it is infused into the portal vein in monovascular perfusion (entry: portal vein;exit: hepatic vein). In the present communication we are presenting and discussing experiments done with the bivascularly perfused rat liver (entry: portal vein + hepatic artery;exit: hepatic vein) in order to verify if the hemodynamic effects also occur in the arterial bed. It was found that the A. blazei extract is also active when infused into the hepatic arterial bed, with differences in both sensitivity and nature of the effects on either perfusion pressure or oxygen consumption. Constriction of the arterial bed required much higher concentrations of the extract than the portal bed. The kinetics of the response was also different, with a biphasic instead of a monophasic response. These results provide a promising starting point for future studies aiming to bring to light more mechanistic details about these and possibly other effects.

关键词： agaricus blazei Purinergic Action Hemodynamics Oxygen Uptake

Study on the Cultivation of agaricus blazei(Almond Mushroom) Grown on Compost Mixed with Selected Agro-Residues

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Advances in Microbiology 2018年第10期8卷 778-789页

作者： Tun Tun Win Shoji Ohga Faculty of Agriculture Kyushu University Fukuoka Japan

The agaricus blazei strain (KUMB 1221) from Forest Production Control Laboratory, Kyushu University was grown on the basal media of compost mixed with selected agro-residues (sawdust, woodchips and corncob) in the ratio of 25%, 50%, 75% and 100% (by weight). The data was collected on the colony diameter of mycelial growth, days required for spawn run (colonization), days required for primordial formation, whiteness measurement, days required for fruit body formation, number, size and fresh weight of mushrooms, biological efficiency (BE) and mushroom production (MP). It was observed that the mycelial growth showed two types of mycelia according the level of mycelial density;compact (C) and somewhat compact (SC). The fastest spawn run (17 days) was found in woodchips (50%) among all the different treatments while the least spawn run took 26 days in corncob (75%). Sawdust substrates promoted longer days for primordial formation and fruit body development, if compared with woodchips substrates and corncob substrates. With regard to yield, 100%, 75% and 50% mixture with compost were superior to 25% mixture with compost in each group of selected agro-residues and it indicated that adding more compost gave the increased yield. Interestingly, it was noted that mushroom size on compost (100%) was double to the mushroom size of other treatments. In conclusion, it was clearly showed that compost (100%), woodchips (25%) and corncob (25%) could produce better yields among all treatments, and it was also possible to obtain acceptable yields of good quality almond mushroom using main substrates of compost mixed with different agro-residues at various concentrations.

关键词： agaricus blazei Mushroom Cultivation Agro-Residues Compost Substrate

Research of Submerge Fermentation Conditions on agaricus blazeiMurill

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Food and Nutrition Sciences 2014年第19期5卷 1926-1943页

作者： Xinzhi Cao Yingqing Zhao Li Xiong Linsheng Ren Jia Liu Department of Bioengineering Sichuan University of Science and Engineering Zigong China

Rotational speed, temperature, inoculation amount and pH are the four factors that affect the submerge fermentation of agaricus blazei Murill. The orthogonal experiment was on the basis of single factor experiments. And the optimal submerge fermentation condition of agaricus blazei Murill was determined through orthogonal test. The results were as follows: temperature (25℃), rotational speed (150 r/min), inoculation amount (6%) and pH (7), in this condition the cell dry weight reached 1.32 g/100mL;temperature (25℃), rotational speed (150 r/min), inoculation amount (8%) and pH (6.5) in this condition the extracellular polysaccharide reached 6.95 mg/mL.

关键词： agaricus blazei Murill Submerged Fermentation Cell Dry Weight Extracellular Polysaccharide

Bioinformatics analysis of metallothionein gene from agaricus bisporus, Ganoderma lucidum, Taiwanofungus camphoratesand Paxillus involutus

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Agricultural Sciences 2012年第7期3卷 900-904页

作者： Cheng-Kui Qiang Bao-Ya Zhou Xiao-Zheng Yang Feng Wei Zheng-Li Lu Song-Song Wang Yue-Hua Qin Sheng-yong Wang Department of Agriculture and Landscape Engineering Xuzhou Vocational College of Bioengineering Xuzhou China Office of Science and Education Xuzhou Agricultural Commission Xuzhou China Xuzhou Key Laboratory of Modern AgroBiotechnology Xuzhou Vocational College of Bioengineering Xuzhou China

Metallothionein (MT) gene has an important role in the detoxification of toxic metals especially some heavy metals. Based on the published sequences of MT genes from agaricus bisporus, Garnoderma lucidum, Taiwanofungus camphoratus and Paxillus involutus as a type of the edible or medical mushroom in NCBI database, their molecular structures, physiological functions and evolutionary relationship were analyzed using the bioinformatics methods. Results showed that AbMT, GlMT, TcMT and PiMT genes contained complete open reading frame (ORF) and their theoretical points of the last three encoding proteins were higher than 7.0. AbMT, GlMT, TcMT and PiMT were relatively rich in random coil and extended strand, but transmembrane helices and signal peptides were not found. 4 MTs were mainly localized in cell nucleus (over 60%) and their cellular functions might have some relation to central intermediary metabolism. Multiple sequence alignment indicated relatively high identity (more than 52%) and short genetic distances (lower than 0.900) among 4 MT nucleotide sequences. Abundant genetic diversity and strong codon bias were found based on the halotype diversity, average number of nucleotide differences, nucleotide diversity, effective number of codons, codon bias index and scaled chisquare. Simultaneously, we deduced that 4 MT genes during molecular evolution were under positive selection. The present study might provide basis for further investigation of MTs molecular mechanisms and genetic laws.

关键词： Metallothionein Gene agaricus bisporus Ganoderma lucidum Taiwanofungus camphoratus Paxillus involutus

Thailand’s amazing diversity:up to 96%of fungi in northern Thailand may be novel

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Fungal Diversity 2018年第6期93卷 215-239页

作者： Kevin D.Hyde Chada Norphanphoun Jie Chen Asha J.Dissanayake Mingkwan Doilom Sinang Hongsanan Ruvishika S.Jayawardena Rajesh Jeewon Rekhani H.Perera Benjarong Thongbai Dhanushka N.Wanasinghe Komsit Wisitrassameewong Saowaluck Tibpromma Marc Stadler Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Instituto de Ecologı́a A.C.CP 91070 XalapaVeracruzMexico World Agro Forestry Centre East and Central Asia132 Lanhei RoadKunming 650201YunnanPeople’s Republic of China Department of Biology Faculty of ScienceChiang Mai UniversityChiang Rai 50200Thailand Shenzhen Key Laboratory of Microbial Genetic Engineering College of Life Sciences and OceanographyShenzhen UniversityShenzhenPeople’s Republic of China Shenzhen Key Laboratory of Laser Engineering College of Optoelectronic EngineeringShenzhen UniversityShenzhenPeople’s Republic of China Department of Health Sciences Faculty of ScienceUniversity of MauritiusReduit 80837Mauritius National Science Technology and Innovation Policy Office Bangkok 10330Thailand Department of Microbial Drugs and German Centre for Infection Research(DZIF) partner site Hannover-BraunschweigHelmholtz Centre for Infection ResearchInhoffenstrasse 738124 BrunswickGermany

Fungi have been often neglected,despite the fact that they provided penicillin,lovastatin and many other important medicines.They are an understudied,but essential,fascinating and biotechnologically useful group of organisms.The study of fungi in northern Thailand has been carried out by us since 2005.These studies have been diverse,ranging from ecological aspects,phylogenetics with the incorportation of molecular dating,taxonomy(including morphology and chemotaxonomy)among a myriad of microfungi,to growing novel mushrooms,and DNA-based identification of plant pathogens.In this paper,advances in understanding the biodiversity of fungi in the region are discussed and compared with those further afield.Many new species have been inventoried for the region,but many unknown species remain to be described and/or catalogued.For example,in the edible genus agaricus,over 35 new species have been introduced from northern Thailand,and numerous other taxa await description.In this relatively well known genus,93%of species novelty is apparent.In the microfungi,which are relatively poorly studied,the percentage of novel species is,surprisingly,generally not as high(55–96%).As well as Thai fungi,fungi on several hosts from Europe have been also investigated.Even with the well studied European microfungi an astounding percentage of new taxa(32–76%)have been discovered.The work is just a beginning and it will be a daunting task to document this astonishingly high apparent novelty among fungi.

关键词： agaricus Amanita Colletotrichum Cornus Fungal diversity Pandanaceae Rosaceae Rosa Teak fungi

agaricus中提取并精制活性成分及其纯度的评价

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天津药学 1998年第3期10卷 13-17页

作者：张莉大野尚仁天津市药品检验所天津300070 日本东京药科大学

从agaricus中提取活性成分──过氧化物酶，并加以精制，其活性通过TMB基质测定。精制后的活性成分分别采用SDS—聚丙烯酰胺凝胶电泳、毛细管电泳和飞行时间型质谱进行评价。

关键词： agaricus 过氧化物酶 TMB 毛细管电泳活性

Major clades in tropical agaricus

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Major clades in tropical Agaricus