A bacterium strain HS-03 was isolated from the activated sludge plant in a suburb of Wuhan city, China which has been found to be capable of aerobic denitrification. The effect of different inoculated amounts of pre-c...
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A bacterium strain HS-03 was isolated from the activated sludge plant in a suburb of Wuhan city, China which has been found to be capable of aerobic denitrification. The effect of different inoculated amounts of pre-cultured media was analyzed. The denitrifying rates of this strain under aerobic and micro-anaerobic conditions were compared. The result indicated that the rate was almost not affected by the presence of oxygen. 10m M nitrate was removed more than 90% in 36 hours by this bacterium in the specific medium. During the denitrifying process, nitrite was always kept at very low level. The denitrification potential of this bacterium was evaluated to treat artificial wastewater under fully aerobic conditions. Main biochemical and physiological features of this strain were characterized. The 16S rDNA sequence was compared with the published data in GenBank by using BLAST. The BLAST level of similarity to Pseudomonas stutzeri was 99.1%. These results of phenotype and genotype proved that the strain HS-03 was a new strain belonging to the species of P. stutzeri.
Objective Screening aerobic denitrification strain has a very important theoretical and practical significance on the industrial wastewater treatment and environment protection.Methods:In order to identify the aerobic...
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Objective Screening aerobic denitrification strain has a very important theoretical and practical significance on the industrial wastewater treatment and environment protection.Methods:In order to identify the aerobic denitrification strain,brucine was used to detective residual nitrate,while using methyl orange bleaching to detective residual nitrite.Results:The results showed that HS-N25 has strong denitrification capacity in specific medium SC,could remove 10mM nitrate in 18 hours.denitrification rate could reach 100%,and did not accumulate nitrite during denitrification processes.Degradation rate of HS-N25 in the artificial wastewater AWW was 72.8% in 24 hours and the nitrate could be completely removed in 60h,but the nitrite was accumulated in degradation process. Conclusions:These results showed HS-N25 had a strong capacity in aerobic denitrification,and present a certain value for the treatment of industrial wastewater.
The denitrification process was traditionally thought to occur only under anaerobic conditions. However, in recent years, aerobic denitrification has been found as a new way to remove nitrogen from wastewater. The tec...
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The denitrification process was traditionally thought to occur only under anaerobic conditions. However, in recent years, aerobic denitrification has been found as a new way to remove nitrogen from wastewater. The technology of aerobic denitrification applying to treat wastewater makes it possible for denitrifying and nitrifying to occur at the same time in one reactor, which reduced the practice cost and difficulties greatly. There are plenty of aerobic denitrifying bacteria in nature. This paper reported that a bacterium strain HS-03 isolated
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