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Identification and Distribution of Bacillus thuringiensis Isolates from Primeval Forests in Yunnan and Hainan Provinces and Northeast Region of China

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Agricultural Sciences in China 2007年第11期6卷 1343-1351页

作者： SU Xu-dong SHU Chang-long ZHANG Jie HUANG Da-fang TAN Jian-xin SONG Fu-ping State Key Laboratory of Biology for Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100094 P.R.China Agricultural University of Hebei Baoding 071001 P.R.China Institute of Biotechnology Research Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China

Ninety-two Bacillus thuringiensis isolates were screened from 683 soil samples collected from tropical and semitropical primeval forests in Yunnan and Hainan provinces of China. Several shapes of crystals, including bipyramidal, square, ovoid, spherical, and amorphous, were observed in the B. thuringiensis isolates. Twenty-six pairs of primers were used to identify 31 holotype cry genes at primary rank of the B. thuringiensis cry gene nomenclature system. The cry gene-types of 92 B. thuringiensis isolates and 33 B. thuringiensis isolates screened from Northeast region of China were identified by PCR-RFLP and SDS-PAGE methods. Fifty-eight isolates harbored cry1 genes, 32 isolates cry2 genes, 12 isolates cry8 genes, 3 isolates cry9 genes, 12 isolates cry11 genes, and 13 isolates cry30 genes. Of the tested isolates, 42 produced no reaction product with 26 pairs of primers and also exhibited no toxicity against 8 insect species tested. The isolate Z2-34 harbored a novel cry30 gene, exhibited insecticidal activity against aedes albopictus of Dipterans. The accession number of the novel genes in this study is AY916046. Isolation and identification of B. thuringiensis and cry gene are important for investigating the diversity of B. thuringiensis resources and cloning new cry gene.

关键词： Bacillus thuringiensis PCR-RFLP cry gene aedes albopictus

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Defervescent dengue patients might be a potential source of infection for vector mosquitoes

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Infectious Diseases of Poverty 2020年第2期9卷 104-104页

作者： Ye Xut Ling-Zhai Zhao Ya-Zhou Xu Jin-Bao Gu Kun Wu Zhi-Qiang Peng Xiao-Hong Zhou Fu-Chun Zhang Xiao-Guang Chen Department of Pathogen Biology School of Public HealthSouthern Medical UniversityGuangzhouChina Guangzhou Eighth People's Hospital Guangzhou Medical UniversityGuangzhouChina 不详

Background:Dengue is a re-emerging public health problem and mosquito-borne infectious disease that is transmitted mainly by A edes aegypti and *** diagnosis,isolation,and treatment of patients are critical steps for dengue epidemic control,especially to prevent secondary transmission of dengue virus(DENV).However,little is known about defervescent dengue patients as a source of ***:This case study describes 1268 dengue patients hospitalized at Guangzhou Eighth People's Hospital from June 2013 to December *** viral loads of each individual were measured using real-time reverse transcription-polymerase chain *** and *** were exposed to blood meal with gradated dengue viral loads to characterize the relationship between viremia in dengue patients and the vector competence of vector ***:The viral numbers in the blood were measured,ranging from 108 to 103 copies/ml from day 1 to day 12 after fever onset Vector competence analysis o fA *** and *** indicated that viremia>104 copies/ml can still infect vector mosquitoes,which implied that the defervescent dengue patients might be a source of ***:The results of this study indicate that some defervescent dengue patients still have sufficient viral load to infect vector ***,the protection against mosquito biting for these people should be extended to prevent secondary transmission events.

关键词： Dengue fever Dengue virus Vector aedes aegypti aedes albopictus

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First identification ofallele F1534S in VGSC gene and its association with resistance to pyrethroid insecticides inpopulations from Haikou City, Hainan Island, China

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贫困所致传染病（英文） 2016年第3期

Background: aedes albopictus is distributed widely in China, as a primary vector of Dengue fever and Chikungunya fever in south of China. Chemical insecticide control is one of the integrated programmes to prevent mosquito-borne diseases. Long-term applications of pyrethroids have resulted in the development of resistance inAe. albopictus populations in China. However, the susceptibility ofAe. albopictus to pyrethroids in Hainan Island was unclear. Knockdown resistance (kdr), caused by point mutations in the VGSC gene, is one of the mechanisms that confer resistance to DDT and pyrethroids. This study was to investigate the resistance level ofAe. albopictus populations in Haikou City to three pyrethroid insecticides, and elucidate the relationship between the resistant phenotype andkdr ***: Theaedes albopictus samples were collected in Xinbu Island (XI), Longtang Town (LT), Shishan Town (ST), Baishamen Park (BP), and Flower Market (FM) from Haikou City, Hainan Island, China. The larval susceptibility to deltamethrin, permethrin and beta-cypermethrin was tested by larval bioassays, and adult susceptibility to deltamethrin and DDT was determined by adult bioassays. The degree of resistance was determined by resistance ratio value (RR50 > 3) for larvae and by mortality for adult. Thekdr alleles at codon 1534 of the VGSC gene were genotyped. The relationship betweenkdr genotypes and resistant phenotypes was analyzed by Chi-square ***: Out of five populations, assessed by larval bioassays, XI was susceptible to deltamethrin and permethrin; LT was susceptible to permethrin and beta-cypermethrin; and ST was susceptible to permithrin. FM and BP both were resistant to all of the three pyrethroids, and FM showed the highest degree of resistance, with RR50 values from 65.17 to 436.36. A total of 493 individuals from the larval bioassays were genotyped forkdr alleles. Five alleles were detected, including two wildtype alleles, TTC(F) (67.04 %) and TTT(F) (

关键词： aedes albopictus Pyrethroids Resistance kdr mutation China

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