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Larvicidal activities of hydro-ethanolic extracts of three Cameroonian medicinal plants against aedes albopictus

Asian Pacific Journal of Tropical Biomedicine 2016年第11期6卷 931-936页

作者： Tankeu Nzufo Francine Biapa Nya Prosper Cabral Pieme Constant Anatole Moukette Moukette Bruno Nanfack Pauline Ngogang Yonkeu Jeanne Laboratory of Biochemistry Department of Biochemistry and Physiological Sciences Faculty of Medicine and Biomedical Sciences University of Yaoundé I Laboratory of Sustainable Management of the Agro-ecosystems ENEA-Italian National Agency for New Technologies Energy and Sustainable Economic Development Laboratory of Medicinal Plant Biochemistry Food Science and Nutrition Department of Biochemistry Faculty of ScienceUniversity of Dschang

Objective: To investigate the larvicidal activity of Syzygium guineense(Myrtaceae)(S. guineense), Monodora myristica and Zanthoxylum heitzii(Rutaceae)(Z. heitzii) extracts against aedes albopictus(Ae. albopictus).Methods: The larvicidal activity of the hydro-ethanolic extracts from these plant species was assessed at three different concentrations(50, 100 and 200 mg/L) on first-instar of Ae. albopictus larvae in comparison with untreated controls. Mortality rate was recorded daily for a period of 12 days. The values of LC_(50) and lethal time killing 50% of the tested individuals(LT_(50)) were calculated using the log-probit ***: The root extract of S. guineense exhibited the best activity with 100% mortality after 8 days of treatment at 200 mg/L, followed by the fruit extract of Z. heitzii with83.33% mortality at the same concentration. Nonetheless, larvae were most susceptible to the fruit extract of Z. heitzii both in terms of LC_(50)(39.89 mg/L) and LT_(50)(145.68 h). A statistically significant difference between the control and the group treated at 200 mg/L was noticed in all the ***: The present study shows that the hydro-ethanolic extracts of S. guineense,Monodora myristica and Z. heitzii tested have significant larvicidal activity. These preliminary results are of great interest and some of these plant species can be proposed for the formulation of new bioinsecticides to control Ae. albopictus populations.

关键词： aedes albopictus Larvicides Plant extracts Syzygium guineense Zanthoxylum heitzii

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Correlation between adult pyrethroid resistance and knockdown resistance(kdr)mutations in aedes albopictus(Diptera:Culicidae)field populations in China

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Infectious Diseases of Poverty 2018年第1期7卷 870-878页

作者： Jing-Peng Gao Han-Ming Chen Hua Shi Heng Peng Ya-Jun Ma Department of Tropical Infectious Diseases Second Military Medical UniversityShanghai 200433China Institute of Disease Control and Prevention People’s Liberation Army of ChinaBeijing 100071China Department of Medical Microbiology and Parasitology College of Basic Medical SciencesSecond Military Medical UniversityShanghai 200433China

Background:Arboviral disease transmitted by aedes albopictus such as dengue fever is an important threat to human *** resistance raises a great challenge for mosquito control.A systematic assessment of *** resistance status in China is urgently needed,and the study of correlation between pyrethroid resistance and knockdown resistance(kdr)mutations would provide information to guide the control of the *** ***:Five field populations of *** were collected from Jinan(JN),Hangzhou(HZ),Baoshan(BS),Yangpu(YP)and Haikou(HK),China in ***-impregnated papers were prepared with four pyrethroid chemicals,deltamethrin,permethrin,beta-cypermethrin and *** susceptibility of *** to pyrethroids was tested by the WHO tube *** mutations were identified by PCR and ***,the correlation analysis between kdr alleles and pyrethroid resistance was ***:All five populations of *** showed resistance to four pyrethroid *** kdr mutant allele at codon 1532 and three at 1534 were detected with frequency of 5.33%(I1532T),44.20%(F1534S),1.83%(F1534 L)and 0.87%(F1534C),*** 1532 and 1534 mutation mosquitoes were found in the BS and YP *** I1532T was negatively correlated with deltamethrin resistance phenotype(OR1).Conclusions:The five field populations of *** adults were all resistant to deltamethrin,permethrin,betacypermethrin and *** F1534S was clearly associated with pyrethroid resistance phenotype in *** and this could be developed as a molecular marker to monitor the pyrethroid resistance problem in China.

关键词： aedes albopictus Pyrethroid Insecticide resistance kdr mutation WHO tube bioassay

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Photoperiodic diapause in a subtropical population of aedes albopictus in Guangzhou,China:optimized fieldlaboratory-based study and statistical models for comprehensive characterization

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Infectious Diseases of Poverty 2018年第1期7卷 903-915页

作者： Dan Xia Xiang Guo Tian Hu Li Li Ping-Ying Teng Qing-Qing Yin Lei Luo Tian Xie Yue-Hong Wei Qian Yang Shu-Kai Li Yu-Ji Wang Yu Xie Yi-Ji Li Chun-Mei Wang Zhi-Cong Yang Xiao-Guang Chen Xiao-Hong Zhou Department of Pathogen Biology Key Laboratory of Prevention and Control for Emerging Infectious Diseases of Guangdong Higher InstitutesGuangdong Provincial Key Laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical UniversityGuangzhou 510515GuangdongChina WHO Collaborating Centre for Infectious Disease Epidemiology and Control School of Public HealthLi Ka Shing Faculty of MedicineThe University of Hong KongHong KongSpecial Administrative RegionChina Guangzhou Center for Disease Control and Prevention Guangzhou 510515China

Background:aedes albopictus is among the 100 most invasive species worldwide and poses a major risk to public *** diapause provides a crucial ecological basis for the adaptation of this species to adverse *** is the vital vector transmitting dengue virus in Guangzhou,but its diapause activities herein remain ***:In the laboratory,yeast powder and food slurry were compared for a proper diapause determination method,and the critical photoperiod(CPP)was tested at illumination times of 11,11.5,12,12.5,13,and 13.5 h.A 4-parameter logistic(4PL)regression model was selected to estimate the *** the field,the seasonal dynamics of the *** population,egg diapause,and hatching of overwintering eggs were investigated monthly,weekly,and daily,respectively.A distributed lag non-linear model(DLNM)was used to assess the associations of diapause with meteorological ***:In the laboratory,both the wild population and the Foshan strain of *** were induced to diapause at an incidence greater than 80%,and no significant difference(P>0.1)was observed between the two methods for identifying *** CPP of this population was estimated to be 12.312 h of *** the field,all of the indexes of the wild population were at the lowest levels from December to February,and the Route Index was the first to increase in *** incidence displayed pronounced seasonal *** was estimated that the day lengths of 12.111 h at week2016,43 and 12.373 h at week2017,41 contributed to diapause in 50%of the *** length was estimated to be the main meteorological factor related to ***:Photoperiodic diapause of *** in Guangzhou of China was confirmed and comprehensively elucidated in both the laboratory and the *** eggs are the main form for overwintering and begin to hatch in large quantities in March in ***,this study also established an optimized

关键词： Subtropical aedes albopictus Critical photoperiod Photoperiodic diapause Distributed lag non-linear model

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Larvicidal activity of Xenorhabdus and Photorhabdus bacteria against aedes aegypti and aedes albopictus

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Asian Pacific Journal of Tropical Biomedicine 2018年第1期8卷 31-36页

作者： Apichat Vitta Punnawat Thimpoo Wipanee Meesil Thatcha Yimthin Chamaiporn Fukruksa Raxsina Polseela Bandid Mangkit Sarunporn Tandhavanant Aunchalee Thanwisai Department of Microbiology and Parasitology Faculty of Medical Science Naresuan University Centre of Excellence in Medical Biotechnology Naresuan University Department of Microbiology and Immunology Faculty of Tropical Medicine Mahidol University Department of Veterinary Technology Faculty of Veterinary Technology Kasetsart University

Objective: To evaluate the efficacy of symbiotic bacteria, Xenorhabdus indica, Xenorhabdus stockiae, Photorhabdus luminescens subsp. akhurstii and Photorhabdus luminescens subsp. hainanensis as a larvicide against aedes aegypti and aedes albopictus. Methods: Larvae(L3-L4) of aedes aegypti and aedes albopictus were given 2 m L of a suspension 107-108 CFU/m L of each symbiotic bacterium. Distilled water and Escherichia coli ATCC襅25922 were used as the control. The mortality rate of the larval mosquitoes was observed at 24, 48, 72 and 96 h. The experiment was performed in triplicates. Results: The larvae of both aedes species started to die at 24 h exposure. aedes aegypti showed the highest mortality rate(87%-99%), 96 h after exposure to Xenorhabdus stockiae(b NBP22.2_TH). The mortality rate of aedes albopictus was between 82% and 96% at 96 h after exposure to Xenorhabdus indica(b KK26.2_TH). Low effectiveness of distilled water and Escherichia coli ATCC襅25922 were observed in both aedes larvae, with a mortality rate of 2% to 12%. Conclusions: The study confirms the oral toxicity of Xenorhabdus and Photorhabdus bacteria against aedes spp. Xenorhabdus stockiae and Xenorhabdus indica may be an alternative agent for control aedes spp. This is basic information for further study on the mechanism of action on aedes larvae or application to control mosquito larvae in the community.

关键词： aedes aegypti aedes albopictus Photorhabdus Xenorhabdus Larvicidal activity

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Seasonality modeling of the distribution of aedes albopictus in China based on climatic and environmental suitability

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Infectious Diseases of Poverty 2020年第1期9卷 103-103页

作者： Xueli Zheng Daibin Zhong Yulan He Guofa Zhou Department of Pathogen Biology School of Public HealthSouthern Medical University1838 Guangzhou North AvenueGuangzhou 510515China 不详

Background:aedes albopictus is a highly invasive mosquito species and a major vector of numerous viral *** recent dengue fever outbreaks in China have been caused solely by the *** of the potential distribution ranges of *** is crucial for epidemic preparedness and the monitoring of vector populations for disease *** is a key factor influencing the distribution of the *** field studies indicating seasonal population variations,very little modeling work has been done to analyze how environmental conditions influence the seasonality of *** aim of the present study was to develop a model based on available observations,climatic and environmental data,and machine learning methods for the prediction of the potential seasonal ranges of *** in ***:We collected comprehensive up-to-date surveillance data in China,particularly records from the northern distribution margin of *** records were assigned long-term(1970–2000)climatic data averages based on the WorldClim 2.0 data *** learning regression tree models were developed using a 10-fold crossvalidation method to predict the potential seasonal(or monthly)distribution ranges of *** in China at high resolution based on environmental *** models were assessed based on sensitivity,specificity,and accuracy,using area under curve(AUC).WorldClim 2.0 and climatic and environmental data were used to produce environmental conduciveness(probability)prediction *** probabilities were generated based on the averages of the 10 ***:During 1998–2017,*** was observed at 200 out of the 242 localities *** addition,at least 15 new *** occurrence sites lay outside the potential ranges that have been predicted using models *** average accuracy was 98.4%(97.1–99.5%),and the average AUC was 99.1%(95.6–99.9%).The predicted *** distribution in win

关键词： aedes albopictus Distribution Northern margin China Climate Environment

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Screening for larvicidal activity of ethanolic and aqueous extracts of selected plants against aedes aegypti and aedes albopictus larvae

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Journal of Coastal Life Medicine 2016年第2期4卷 143-147页

作者： Michael Russelle Alvarez Francisco Heralde III Noel Quiming Department of Biochemistry and Molecular Biology College of MedicineUniversity of the Philippines ManilaManilaPhilippines Department of Physical Sciences and Mathematics College of Arts and SciencesUniversity of the Philippines ManilaManilaPhilippines

Objective:To screen for larvicidal activity of aqueous and ethanolic extracts(95%ethanol)from Selaginella elmeri,Christella dentata,Elatostema sinnatum,Curculigo capitulata,Euphorbia hirta,Murraya koenigii(***),Alpinia speciosa,Cymbopogon citratus,Eucalyptus globulus(***),Jatropha curcas(***),Psidium guajava,Gliricidia sepium,Ixora coccinea and Capsicum frutescens(***)against aedes aegypti(***)and aedes albopictus(***)3rd instar ***:Ethanolic and aqueous extracts were screened for larvicidal activity by exposing the *** and *** 3rd instar larvae(15 larvae per trial,triplicates)for 48 h,counting the mortalities every 24 ***,phytochemical screening for flavonoids,tannins,alkaloids,anthraquinones,anthrones,coumarins,indoles and steroids were performed on active extracts using spray ***:Against ***,the three most active extracts were *** ethanolic(100%after 24 and 48 h),*** ethanolic(84.44%after 24 h and 88.89%after 48 h)and *** ethanolic(53.33%after 24 h and 71.11%after 48 h).On the other hand,against ***,the three most active extracts were *** ethanolic(93.33%after 24 h and 100%after 48 h),*** ethanolic(77.78%after 24 h and 82.22%after 48 h)and *** ethanolic(64.44%after 24 h and 73.33%after 48 h).Phytochemical screening was also performed on the active extracts,revealing alkaloids,tannins,indoles and ***:The results demonstrate the larvicidal activities of ethanolic extracts of Cymbopogon citratus,Euphorbia hirta,Ixora coccinea,Gliricidia sepium,***,***,*** and *** against *** and *** 3rd instar *** could be used as potential larvicidal agents for the control of these mosquitoes.

关键词： Larvicidal screening Capsicum frutescens Jatropha curcas aedes aegypti aedes albopictus

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Cloning and sequence analysis of β-actin gene from aedes albopictus (Diptera: Culicidae)

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Journal of Nanjing Medical University 2007年第2期21卷 63-67页

作者： Weijie Wang Xiaobang Hu Donghui Zhang Jianhua Jiao Yan Sun Lei M＇ Changliang Zhu Department of Pathogen Biology Jiangsu Province Key Laboratory of Modern Pathogen Biology Nanjing Medical University Nanjing 210029 China.

Objective： To obtain the complete β-actin gene from aedes albopictus. Methods： Total RNA was extracted from C6/36 cells. Degenerate primers were designed based on the β-actin sequences of An. gambiae, Ae. aegypti, Cx. pipiens pallens and D. melanogaster. By RT-PCR, the product was amplified, purified, cloned into the pGT vector and sequenced. The β-actin sequence was aligned and phylogenetically analyzed by the BLAST program and the CLUSTAL W program. Results： A sequence of 1132 bp including an open reading frame of 1131 bp was obtained （GenBank DQ657949）. The deduced protein had 376 amino acids. Aligned to SWISS-PROT, it exhibited a high level of identity with β-actins from Anopheles, Drosophila and Culex at the amino acid sequence level. Phylogenetic analysis indicated that Ae. albopictus β-acfin was much more homologous with invertebrate β- actin than with vertebrate β-actin. Conclusion： The gene may be used as the internal control in the experiments of Ae. albopictus.

关键词： aedes albopictus β-actin clone nucleotide sequence phylogeny

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白纹伊蚊(aedes albopictus)细胞株的建立及其特征

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北京第二医学院学报 1980年第1期 10-18页

作者：潘李珍孔德芳王凤芸石梦辉北京第二医学院寄生虫学教研组

从白纹伊蚊(aedes albopictus)初孵幼虫组织建立三个细胞株。其中两个作过系统研究,目前为止传至21、34、44代次。细胞类型以多边形上皮细胞为主(约占90％)掺杂极少数的梭形细胞、双核细胞。细胞生长迅速而恒定,对数期二倍增长时间均为1... 详细信息

从白纹伊蚊(aedes albopictus)初孵幼虫组织建立三个细胞株。其中两个作过系统研究,目前为止传至21、34、44代次。细胞类型以多边形上皮细胞为主(约占90％)掺杂极少数的梭形细胞、双核细胞。细胞生长迅速而恒定,对数期二倍增长时间均为11小时,接种6天细胞能增殖至原来的10倍左右。细胞接种后2小时贴壁,8小时少量分裂,24小时分裂数达高峰。细胞核学特征以二倍体为主(占74—90％)。三个细胞株冷冻贮存于液氮(-196℃)中,一年后仍有94％能复活。对细胞株作外来因子检查,结果阴性。初步试验证明细胞系对六种病毒有敏感性。