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Screening for larvicidal activity of ethanolic and aqueous extracts of selected plants against aedes aegypti and aedes albopictus larvae

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Journal of Coastal Life Medicine 2016年第2期4卷 143-147页

作者： Michael Russelle Alvarez Francisco Heralde III Noel Quiming Department of Biochemistry and Molecular Biology College of MedicineUniversity of the Philippines ManilaManilaPhilippines Department of Physical Sciences and Mathematics College of Arts and SciencesUniversity of the Philippines ManilaManilaPhilippines

Objective:To screen for larvicidal activity of aqueous and ethanolic extracts(95%ethanol)from Selaginella elmeri,Christella dentata,Elatostema sinnatum,Curculigo capitulata,Euphorbia hirta,Murraya koenigii(***),Alpinia speciosa,Cymbopogon citratus,Eucalyptus globulus(***),Jatropha curcas(***),Psidium guajava,Gliricidia sepium,Ixora coccinea and Capsicum frutescens(***)against aedes aegypti(***)and aedes albopictus(***)3rd instar ***:Ethanolic and aqueous extracts were screened for larvicidal activity by exposing the *** and *** 3rd instar larvae(15 larvae per trial,triplicates)for 48 h,counting the mortalities every 24 ***,phytochemical screening for flavonoids,tannins,alkaloids,anthraquinones,anthrones,coumarins,indoles and steroids were performed on active extracts using spray ***:Against ***,the three most active extracts were *** ethanolic(100%after 24 and 48 h),*** ethanolic(84.44%after 24 h and 88.89%after 48 h)and *** ethanolic(53.33%after 24 h and 71.11%after 48 h).On the other hand,against ***,the three most active extracts were *** ethanolic(93.33%after 24 h and 100%after 48 h),*** ethanolic(77.78%after 24 h and 82.22%after 48 h)and *** ethanolic(64.44%after 24 h and 73.33%after 48 h).Phytochemical screening was also performed on the active extracts,revealing alkaloids,tannins,indoles and ***:The results demonstrate the larvicidal activities of ethanolic extracts of Cymbopogon citratus,Euphorbia hirta,Ixora coccinea,Gliricidia sepium,***,***,*** and *** against *** and *** 3rd instar *** could be used as potential larvicidal agents for the control of these mosquitoes.

关键词： Larvicidal screening Capsicum frutescens Jatropha curcas aedes aegypti aedes albopictus

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Mosquito adulticidal properties of Delonix elata(Family:Fabaceae)against dengue vector,aedes aegypti(Diptera:Culicidae)

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Journal of Coastal Life Medicine 2014年第5期 389-393页

作者： Mohan Rajeswary Marimuthu Govindarajan Unit of Vector Biology and Phytochemistry Department of ZoologyAnnamalai UniversityAnnamalai Nagar-608002Tamil NaduIndia

Objective:To determine the adulticidal activity of hexane,benzene,chloroform,ethyl acetate and methanol leaf and seed extracts of Delonix elata(***)against aedes aegypti(***).Methods:The bioassay was conducted in an experimental kit consisting of two cylindrical plastic tubes both measuring 125 mm×44 mm following the WHO method;mortality of the mosquitoes was recorded after 24 ***:The adulticidal activity of plant leaf and seed extracts showed moderate toxic effect on the adult mosquitoes after 24 h of exposure ***,the highest adulticidal activity was observed in the leaf methanol extract of *** against *** with the LC_(50) and LC_(90) values 162.87 and 309.32 mg/L,***:From this result,it can be concluded the crude extract of *** was an excellent potential for controlling *** mosquitoes.

关键词： Delonix elata Adulticidal activity aedes aegypti

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Discovery and high prevalence of Phasi Charoen-like virus in field-captured aedes aegypti in South China

Discovery and high prevalence of Phasi Charoen-like virus in...

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2018新发传染病研究热点研讨会

作者：张晓敏 Suibin Huang Tao Jin Shenzhen Center for Disease Control and Prevention School of Public Health and Tropical MedicineSouthern Medical University School of Public HealthShanxi Medical University

Arboviruses have caused significant global health concerns during the past *** this regard,continuous viral surveillance is essential to timely identify emerging arboviruses and other novel viruses. Here,a novel isolate of Phasi Charoen-like virus (PCLV Zhanjiang01) was identified from field-captured aedes aegypti mosquitoes in Zhanjiang by next generation sequencing. Phylogenetic analysis suggested that PCLV Zhanjiang01 belonged to the genus Phasivirus in the family Phenuiviridae. The presence of PCLV in three batches of aedes aegypti confirmed its high prevalence in nature. Further detection of PCLV in progenies and adult males suggested vertical transmission in mosquitoes. In parallel,PCLV was detected from multiple organs indicating its broad tissue distribution in the infected mosquitoes. To the best of our knowledge,this is the first report of PCLV in *** results expanded the global biogeographic distribution of PCLV. Further investigations of PCLV on the arboviral transmission and control strategies are warranted.

关键词： Phasi Charoen-like virus Bunyavirus Insect-specific virus aedes aegypti

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我国白纹伊蚊(aedes albopictus)与埃及伊蚊(aedes aegypti)对杀虫剂的抗药性

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医学动物防制 1988年第2期 5-8页

作者：吴能陈达宗广西壮族自治区寄生虫病防治研究所

引言白纹伊蚊和埃及伊蚊这两种伊蚊都是黄热病、登革热等的虫媒病毒病的重要传播媒介。自从1981年登革热在我国沿海地区暴发流行后,在中央爱卫会领导下,全国省(市)级卫生防疫部门与科研单位组织了协作组,

关键词：抗药性敏感度 DDT 耐药性埃及伊蚊伊蚊属白纹伊蚊 aedes aegypti aedes albopictus

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Virtual Screening and Molecular Docking for Arylalkylamine-N-Acetyltransferase(aaNAT)Inhibitors,a Key Enzyme of aedes(Stegomyia)aegypti(L.)Metabolism

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Computational Molecular Bioscience 2015年第3期5卷 35-44页

作者： Bruno Luis Alves Lourenco Maicon Vinícius Araújo Santos Silva Elisson Barros de Oliveira Wagner Rodrigues de Assis Soares Aristóteles Góes-Neto Gesivaldo Santos Bruno Silva Andrade Departamento de Saúde II Universidade Estadual do Sudoeste da Bahia (UESB) Jequié Brasil Departamento de Ciências Biológicas Universidade Estadual de Feira de Santana (UEFS) Feira de Santana Brasil Departamento de Ciências Biológicas Universidade Estadual do Sudoeste da Bahia (UESB) Jequié Brasil

Background: Dengue is a Neglected tropical disease (NTDs) with high incidence in Brazil. This disease is caused by Dengue virus and is transmitted by aedes aegypti mosquito. The search for new approaches for controlling of this disease is the subject of numerous studies. The aaNAT is a key enzyme in the metabolism of A. aegypti and is crucial in the sclerotization process, as well as regulation of circadian rhythm and inactivation of neurotransmitters. Computational techniques applied to studies of biological systems become an effective weapon in the mapping and management of 3D data structures, giving direction and guidance of potential ligands that can form stable complexes with targets of interest, using a Molecular Docking approach. The present study was conducted by a virtual screening, followed by docking calculations, in order to find molecules that could inhibit aaNAT. In this study, we used available compounds in SAM database (Bioinformatics and Medicinal Chemistry Laboratory—Southwest Bahia State University, Jequié-Bahia, Brazil), PubChem and ZINC. Results: The result of dockings with selected ligands showed good energy affinities, presenting potential inhibitory interactions with the enzyme active site. Conclusions: The Coa-S-acetyl-tryptamine and 3-indoleacriloil-coenzyme-A showed the same binding energies -8.9 Kcal/Mol and were described as possible inhibitors of aaNAT.

关键词： aedes aegypti aaNAT Virtual Screening Molecular Docking

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Assessing the risk of spread of Zika virus under current and future climate scenarios

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Biosafety and Health 2022年第3期4卷 193-204页

作者： Ye Xu Jingni Zhou Tong Liu Peiwen Liu Yang Wu Zetian Lai Jinbao Gu Xiaoguang Chen Department of Pathogen Biology Key Laboratory of Tropical Disease Research of Guangdong ProvinceSchool of Public HealthSouthern Medical UniversityGuangzhou 510515China Institute of Tropical Medicine School of Public HealthSouthern Medical UniversityGuangzhou 510515China

Zika virus(ZIKV)may cause severe microcephaly in newborn babies and Guillain‐Barrésyndrome in some *** recent decades,its range has expanded in 86 *** are two ecologically and evolutionarily district cycles:urban cycle and sylvatic *** work aimed to estimate the urban and sylvatic cycle areas of ZIKV throughout the *** occurrence records of vectors,non‐human primate hosts,and ZIKV were *** chose historical climate data,predicted vectors distribution,human population density,and elevation data as the variables to fit the maximum entropy model(MaxEnt).Current risk area and future prediction were performed with global climate models(GCMs)and shared socioeconomic pathways(SSPs).Predicting the ZIKV risk area would help tailor related control *** results indicated that 16.6%of the world’s landmass(except Antarctica)is a risk area in the urban *** 6.22 billion people(78.69%of the global population)live in the risk area,with the vast majority in South Asia,tropical Africa,South America,North America,and countries around the Mediterranean *** climate change decreases the risk area of *** study also suggested that the sylvatic cycle happened between the Tropic of Cancer and the Tropic of *** overlap region of the urban and sylvatic cycles could be hotpots that ZIKV spill from the sylvatic to the urban *** is indicated that long‐term passenger screening,mosquito surveillance,and control are necessary.

关键词： Zika virus aedes aegypti aedes albopictus Species distribution modeling MaxEnt Urban cycle Sylvatic cycle

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Seropositivity of dengue cases at a tertiary care centre in Chhindwara,Madhya Pradesh:A three year trend

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Infectious Medicine 2023年第1期2卷 44-48页

作者： Purti C.Tripathi Himanshu Singh Rahul Kumar Suryawanshi Ritesh Upadhyay Department of Microbiology Chhindwara Institute of Medical SciencesChhindwaraMadhya Pradesh 480001India Department of Microbiology Dr.Harisingh Gour Vishwavidyalaya(A Central University)SagarMadhya Pradesh 470003India Department of Community Medicine Chhindwara Institute of Medical SciencesChhindwaraMadhya Pradesh 480001India

Background:During the recent decades,dengue virus infection has been emerged as a major public health *** is one of the important mosquito borne infections causing high mortality and morbidity of ***:This study was carried out from October 2018 to December 2020.A total of 354 serum samples of clinically suspected dengue patients were tested for immunoglobulin M(IgM)anti-dengue antibodies by dengue monoclonal antibody capture enzyme-linked immunosorbent assay(NIV DEN IgM Capture MAC ELISA).Seasonal variations,age and sex wise incidences were also ***:Total of 354 serum samples were processed from October 2018 to December *** year males were mostly infected with Dengue 08,10,and 03 in 2018,2019,and 2020,*** group 11-20 was found to be mostly infected by Dengue in case of both male and *** prevalence of Dengue in each year was from July to November might be due to the rainy *** characteristics of patients such as fever,headache,abdominal pain and nausea/vomiting,retro-orbital pain,epistaxis,petechiae,altered sensorium,positive tourniquet test were ***:Continuous dengue virus surveillance is required for monitoring of dengue virus so that early detec-tion can be carried *** vector control measures should be implemented for early detection of impending outbreak and to initiate timely control measures.

关键词： Dengue Seropositivity aedes aegypti IgM ELISA

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Developing Exon-Primed Intron-Crossing （EPIC） markers for population genetic studies in three aedes disease vectors

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Insect Science 2015年第3期22卷 409-423页

作者： Vanessa Linley White Nancy Margaret Endersby Janice Chan Ary Anthony Hoffmann Andrew Raymond Weeks Department of Genetics Bio21 Institute the University of Melbourne Victoria 3010 Australia

aedes aegypti, aedes notoscriptus, and aedes albopictus are important vectors of many arboviruses implicated in human disease such as dengue fever. Genetic markers applied across vector species can provide important information on population structure, gene flow, insecticide resistance, and taxonomy, however, robust microsatellite markers have proven difficult to develop in these species and mosquitoes generally. Here we consider the utility and transferability of 15 Ribosome protein （Rp） Exon-Primed Intron- Crossing （EPIC） markers for population genetic studies in these 3 aedes species. Rp EPIC markers designed for Ae. aegypti also successfully amplified populations of the sister species, Ae. albopictus, as well as the distantly related species, Ae. notoscriptus. High SNP and good indel diversity in sequenced alleles plus support for amplification of the same regions across populations and species were additional benefits of these markers. These findings point to the general value of EPIC markers in mosquito population studies.

关键词： aedes aegypti aedes albopictus aedes notoscriptus Exon-Primed Intron- Crossing (EPIC) indels Ribosome protein (Rp) SNPs

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Satyrization and satyrization-resistance in competitive displacements of invasive mosquito species

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Insect Science 2016年第2期23卷 162-174页

作者： Irka Ewa Bargielowski Leon Philip Lounibos Florida Medical Entomology Laboratory University of Florida Vero Beach Florida USA

Competitive displacements or reductions of resident populations of insects, often effected by a related species, may be caused by a variety of mechanisms. Satyrization is a form of mating interference in which males of one species mate with females of another species, significantly decreasing their fitness and not generating hybrids. Satyriza- tion has been established to be the probable cause of competitive displacements of resident mosquitoes by invasive species, especially of aedes aegypti by aedes albopictus, two important vectors of dengue and chikungunya viruses. Mathematical models predict that even low levels of asymmetric mating interference are capable of producing competitive displacements or reductions. Couplings of virgin Ae. aegypti females with Ae. albopictus males effectively sterilize these females through the monogamizing actions of male ac- cessory gland products, but the converse interspecific mating does not impact the future reproduction ofAe. albopictus females. Populations ofAe. aegypti exposed to satyrization quickly evolve resistance to interspecific mating, which is believed to ameliorate repro- ductive interference from, and promote co-existence with, Ae. albopictus. The evolution of satyrization resistance among Ae. aegypti in laboratory cages is accompanied by fitness costs, such as reduced fecundity and slower receptivity to conspecific males. Cage exper- iments and field observations indicate that Ae. albopictus males are capable of satyrizing females of other species of the Stegomvia subgenus, potentially leading to competitive displacements, and possible extinctions, especially of endemic species on islands. Exam- ination of other examples of reproductive interference in insects reveals few parallels to the mechanism and outcomes of satyrization by Ae. albopictus. We conclude by posing the hypothesis that satyrization may favor the ecological success of Ae. albopictus, and suggest many lines for future research on this phenomenon.

关键词： aedes aegypti aedes albopictus mating interference reproductive interfer-ence satyrization

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Susceptibility and interactions between aedes mosquitoes and Zika viruses

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Insect Science 2021年第5期28卷 1439-1451页

作者： Teng-Fei Zhou Ze-Tian Lai Shuang Liu Jia-Yong Zhou Yang Liu Yang Wu Ye Xu Kun Wu Jin-Bao Gu Gong Cheng Xiao-Guang Chen Department of Pathogen Biology Key Laboratory of Tropical Disease Research of Guangdong ProvinceSchool of Public HealthSouth-ern Medical UniversityGuangzhouChina Tsinghua-Peking Center for Life Sciences School of MedicineTsinghua UniversityBeijing100084China Department of Biochemistry&Molecular Biology University of Texas Medical BranchGalvestonTexasUSA

Zika virus disease is caused by Zika virus infection,as transmitted by aedes *** of the Zika virus strains isolated from patients display different pathogenicities toward *** vector mosquitoes for Zika virus are mainly of the aedes genus,especially aedes aegypti and aedes ***,susceptibility and interactions between aedes *** and Zika viruses remain *** this study,we chose two Zika virus strains(FSS 13025 and PRVABC59)with different abilities to infect the primary vector mosquitoes *** and *** transcriptomes and small RNA profiles of infected and uninfected mosquitoes were comparatively analyzed,and differentially expressed genes were functionally examined using RNA *** to the results,the susceptibility of PRVABC59 was higher than that of FSS13025 in aedes vector mosquitoes,and *** was more susceptible to Zika virus than was *** PRVABC59 infection,specific differential expression profiles correlated with *** and ***,and susceptibility was significantly affected when three targeted genes were successfully knocked *** with PRVABC59,infection of *** with FSS 13025 generated more 21-nt virus small interference *** can be concluded that the susceptibility of vector aedes *** to Zika viruses varies and that the interactions between mosquitoes and Zika virus correlate with susceptibility.

关键词： aedes aegypti aedes albopictus RNAi RNA-Seq susceptibility Zika virus

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