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Susceptibility of aedes flavopictus miyarai and aedes galloisi mosquito species in Japan to dengue type 2 virus

Asian Pacific Journal of Tropical Biomedicine 2016年第5期6卷 446-450页

作者： Raweewan Srisawat Thipruethai Phanitchat Narumon Komalamisra Naoki Tamori Lucky Runtuwene Kaori Noguchi Kyoko Hayashida Shinya Hidano Naganori Kamiyama Ikuo Takashima Tomohiko Takasaki Ichiro Kurae Narihiro Narita Takashi Kobayashi Yuki Eshita Faculty of Tropical Medicine Mahidol University Faculty of Medicine Oita University Laboratory of Public Health Department of Environmental Veterinary SciencesGraduate School of Veterinary MedicineHokkaido University Department of Virology 1 National Institute of Infectious Diseases Cultural Anthropology Laboratory Department of Arts and SciencesOhkagakuen University

Objective: To evaluate the potential of local mosquitoes to act as vectors for dengue transmission in Japan.Methods: Serotype 2 Th NH28/93 was used to test the dengue susceptibility profiles of aedes flavopictus miyarai(Ae. f. miyarai), aedes galloisi(Ae. galloisi) and aedes albopictus(Ae.albopictus), which were collected in Japan. We used aedes aegypti from Thailand as a positive control. The mosquitoes were infected with the virus intrathoracically or orally. At 10 or 14 days post infection, the mosquitoes were dissected and total RNA was extracted from their abdomens, thoraxes, heads and legs. Mosquito susceptibility to dengue virus was evaluated using RT-PCR with dengue virus-specific primers. Differences in the infection and mortality rates of the different mosquito species were tested using Fisher's exact probability test.Results: The infection rates for dengue virus administered intrathoracically to Ae. f. miyarai,Ae. galloisi and aedes aegypti mosquitoes were identical by RT-PCR on Day 10 post infection.All of the body parts we tested were RT-PCR-positive for dengue virus. For the orally administered virus, the infection rates in the different body parts of the Ae. f. miyarai mosquitoes were slightly higher than those of Ae. albopictus mosquitoes, but were similar to the control mosquitoes(P > 0.05). The mortality rates for Ae. f. miyarai and Ae. albopictus mosquitoes were similar(P = 0.19). Our data indicated that dengue virus was able to replicate and disseminate to secondary infection sites in all of the four mosquito species(Japanese and Thai).Conclusions: Ae. albopictus is a well-known candidate for dengue transmission in Japan. However, our data suggest that Ae. f. miyarai from Ishigaki Island(near Okinawa Island) and Ae. galloisi from Hokkaido(Northern Japan) should also be regarded as potential vectors for dengue transmission in these regions. Further studies on these mosquitoes should be conducted.

关键词： aedes flavopictus miyarai aedes galloisi aedes albopictus aedes aegypti Dengue type 2 virus Japan Oral infection Intrathoracic inoculation

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Bioefficacy of Mentha piperita essential oil against dengue fever mosquito aedes aegypti L

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Asian Pacific Journal of Tropical Biomedicine 2011年第2期1卷 85-88页

作者： Sarita Kumar Naim Wahab Radhika Warikoo Department of Zoology Acharya Narendra Dev College(University of Delhi)

Objective:To assess the larvicidal and repellent potential of the essential oil extracted from the leaves of peppermint plant,Mentha piperita(M.piperita)against the larval and adult stages of aedes aegypli(Ae.Aegypti).Methods:The larvicidal potential of peppermint oil was evaluated against early fourth instar larvae of Ae.aegypli using WHO protocol.The mortality counts were made after 24 and 48 h,and LC_(50)and LC_(90)values were calculated.The efficacy of peppermint oil as mosquito repellent was assessed using the human-bait technique.The measured area of one arm of a human volunteer was applied with the oil and the other arm was applied with ethanol.The mosquito bites on both the arms were recorded for 3 rain after every 15 min.The experiment continued for 3 h and the percent protection was calculated.Results:The essential oil extracted from M.piperita possessed excellent larvicidal efficiency against dengue vector.The bioassays showed an LC_(50)and LC_(90)value of 111.9 and 295.18 ppm,respectively after 24 h of exposure.The toxicity of the oil increased 11.8%when the larvae were exposed to the oil for 48h.The remarkable repellent properties of M.piperita essential oil were established against adults Ae.aegypti.The application of oil resulted in 100%protection till 150 min.After next 30 min,only 1-2 bites were recorded as compared with 8-9 bites on the control arm.Conclusions:The peppermint essential oil is proved to be efficient larvicide and repellent against dengue vector.Further studies are needed to identify the possible role of oil as adulticide.oviposition deterrent and ovicidal agent.The isolation of active ingredient from the oil could help in formulating strategies for mosquito control.

关键词： Essential oil Larvicide Repellent %protection Bioefficacy Mentha piperita Dengue aedes aegypti Larvicidal potential Adulticide

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Bioactivity of seagrass against the dengue fever mosquito aedes aegypti larvae

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Asian Pacific Journal of Tropical Biomedicine 2012年第7期2卷 570-573页

作者： M Syed Ali S Ravikumar J Margaret Beula School of Marine Sciences Division of Marine Microbiology and MedicineDepartment of Oceanography and Costal Area StudiesAlagappa UniversityThondi Campus Department of Chemistry Scott Christian College(Autonomous)

Objective:To identify the larvieidal activity of the seagrass extracts.Methods:Seagrass extracts,Syringodium isoelifolium(.S.isoetifolium),Cymodocea serrulata and Hulophila beccarii,were dissolved in DMSO to prepare a graded series of concentration.Batches of 25 early 4th instars larvae of aedes aegypti(Ae.aegypti) were transferred to 250 ml.enamel bowl containing199 mL of distilled water and 1 mL of plant extracts(0.01 mg- 0.1 mg).After 24 h the mortality rate was identified with the formulae[(%of lest mortality-%of control mortality)/(100-%of control morlality)]× 100.Each experiment was conducted with three replicates and a concurrent control group.A control group consisted of 1 mL of DMSO and 199 mL of distilled water only.Results:The root extract of S.isoelifolium showed maximum larvicidal activity with minimum concentration of extract of LC_(50)= 0.0604 ± 0.0040)μg/mL with lower confidence limit(LCL)-upper confidence limit(UCL) =(0.051-0.071) and LC_(90)=0.0972 μg/mL followed by leaf extract of S.isoelifolium showed LC_(50)=(0.062 ± 0.005)μ'g/mL.The regression equation of pool and leaf extract of S.isoelifolium for4th instar larvae were Y =4.909- 1.32x(R^2= 0.909) and Y= 2.066+ 1.2x(R^2 =0.897) respectively.The results of the preliminary photochemical constituents shows the presence of saponin,steroids,terpenoid,phenols,protein and sugars.Conclusions:from the present study the ethanolic extracts of seagrass of S.isoelifolium possesses lead compound for development of larvieidal activity.

关键词： aedes aegypti Bioinsecticides Dengue fever Larvicides Seagrass Syringodium isoetifolium Cymodocea serrulata Halophila beccarii Mosquito Extract

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Toxicity and sub-lethal effect of endemic plants from family Anacardiaceae on oviposition behavior of aedes albopictus

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Asian Pacific Journal of Tropical Biomedicine 2015年第8期5卷 599-604页

作者： Wan Fatma Zuharah Chan Jia Ling Nurfazlina Zulkifly Nik Fadzly School of Biological Sciences Universiti Sains Malaysia Vector Control Research Unit Universiti Sains Malaysia Centre of Marine & Coastal Studies (CEMACS) Universtiti Sains Malaysia

Objective: To evaluate the lethal concentration, oviposition deterrence and ovicidal activity of acetone extracts of Melanochyla fasciculiflora(M. fasciculiflora) leaf and Gluta renghas(G. renghas) leaf against aedes albopictus(Ae. albopictus). Methods: To determine the lethal concentration of Anacardiaceae, ten test concentrations of the extracts ranging from 200 to 650 mg/L were selected for larvicidal bioassays and 25 early fourth instar larvae were exposed to the extracts for 24 h. The sub-lethal concentrations used for oviposition deterrence was the value of LC25, LC50 and LC75 from above study which is 235 mg/L, 470 mg/L and 705 mg/L for M. fasciculiflora extract and 187.5 mg/L, 375 mg/L and 562.5 mg/L for G. renghas extract, respectively. Twenty gravid Ae. albopictus were allowed to oviposit in different treated concentrations. For oviciding procedure, a total of 300 eggs of Ae. albopictus were soaked in solution with each treated concentration as mentioned above for 24 h. After 24 h, eggs were sieved and soaked in seasoned water, and hatching rates were calculated. For comparison, only seasoned water was used in control experiment.Results: G. renghas demonstrated lower LC50 value of 372.80 mg/L compared to M. fasciculiflora(467.90 mg/L). The activity index of negative oviposition revealed the deterrent effect and thus, caused a remarkable negative response resulting in oviposition of fewer eggs compared with control(without plant extract). The acetone extract of M. fasciculiflora was more effective than G. renghas extract in displaying oviposition deterrence potential since the latter did not possess the deterring effect within the concentration range tested. However, both plant extracts exhibited excellent oviciding effect as 92.33% of eggs failed to be hatched when treated with 705.0 mg/L of M. fasciculiflora and 86.67% with 562.5 mg/L of G. renghas. The oviposition deterrence and percentage of egg mortality were directly proportional to the concentrations of extracts in both plants tested. Conclusions:

关键词： aedes Control Larvicide Mosquito Oviposition Plant extract Repellent

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Toxicity of Mexican native plant extracts against larvae of aedes aegypti(Diptera: Culicidae)

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Asian Pacific Journal of Tropical Biomedicine 2015年第4期5卷 287-291页

作者： Rosario Ruiz-Guerrero Mario Alberto Rodríguez-Pérez Mariano Norzagaray-Campos CIITEC-IPN Cerrada de Cecati S/N. Col. Santa Catarina Azcapotzalco Centro de Biotecnología Genómica-IPN Blvd. Del Maestro S/N Colonia Narciso Mendoza CIIDIR-IPN-Sinaloa Bulevar Juan de Dios Bátiz Paredes No 250 Colonia San Joachin. Guasave

Objective: To evaluate five indigenous Mexican plants [Hippocratea excelsa, Hippocratea celastroides, Argemone mexicana(A. mexicana), Tagetes lucida, and Pseudosmodingium perniciosum(P. perniciosum)] toxicity against the fourth instar larvae of the dengue primary vector, aedes aegypti(A. aegypti).Methods: Each plant part was treated successively with hexane, ethyl acetate, acetone, and methanol to extract potential active components of the plants against the dengue vector.Results: There was a range of toxicity at 24 or 48 h post-exposure for the different plant parts and organic solvent used(LC50 values ranged between 20 and 890 μg/mL). Extracts from seeds of A. mexicana(hexane washing with methanol and acetone) and stem-bark of P. perniciosum(hexane) showed highest toxicity to Ae. aegypti larvae at 48 h post-exposure(LC50 values were80, 50, and 20 μg/mL, respectively), thus making them potential candidates as biolarvicides.Efforts are on-going to characterize the bioactive components of the extracts, through chromatography, for their use as biological tools for the control of the primary dengue vector.Conclusions: A. mexicana and P. perniciosum are good candidates to combat the dengue vector, Ae. aegypti, as they were highly toxic to the larvae.

关键词： aedes aegypti Dengue Larvicidal activity Plant organic extracts

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Diversity and species composition of microbiota associated with dengue mosquito breeding habitats:A cross-sectional study from selected areas in Udapalatha MOH division,Sri Lanka

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Asian Pacific Journal of Tropical Medicine 2023年第8期16卷 363-370页

作者： Yashoda Kumari Deepika Amarasinghe Koshila Ranasinghe Department of Zoology and Environmental Management Faculty of ScienceUniversity of KelaniyaDalugamaKelaniya GQ 11600Sri Lanka

Objective:To determine the diversity of microbiota associated with different breeding habitats of dengue vector mosquitoes aedes(Ae.)aegypti and Ae.albopictus and to identify any parasitic,epibiont,pathogenic,competitive or predatory species.Methods:Sampling was performed from a variety of breeding habitats using dipping,pipetting and siphoning techniques.Microbiota in water samples were preserved using Rose Bengal solution and Lugol's iodine,and were identified.Live samples of microbiota were kept under laboratory conditions to observe any pathogenic or parasitic microbiota interacting with larvae.Results:A total of eleven microbiota species(Canthocamptus staphylinus,Canthocamptus microstaphylinus,Parastenocaris brevipes,Lepadella ovalis,Lepadella patella,Rotatoria rotatoria,Rotatoria macrura,Asplanchna brightwelli,Trichocerca rattus,Euglena variabilis,and Flagilaria capucina)belonging to four(4)phyla(Arthropoda,Rotifera,Euglenozoa,and Ochrophyta)and 8 microbiota species belonged to four phyla(Arthropoda,Rotifera,Euglenozoa,and Ochrophyta)were identified from Ae.aegypti and Ae.albopictus breeding habitats respectively.There was a higher percentage(54.54%)of larval habitats positive for the secondary vector Ae.albopictus than through the primary vector Ae.aegypti in the Gampola urban area indicating higher possibility of transmitting the dengue virus through the secondary vector.However,no pathogenic or parasitic ciliates on mosquito larvae were encountered in the present study.Those findings may be due to sampling maingly from temporary container-type breeding habitats.Conclusions:The relative distribution of microbiota associated with mosquito species differed significantly among Ae.aegypti and Ae.albopictus.The overall findings of this study could help in implementing novel eco-friendly vector-control strategies in the study area.

关键词： aedes Biological Mosquito-control Vectors

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aedes mosquito salivary immune peptides:boost or block dengue viral infections

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Journal of Coastal Life Medicine 2014年第2期2卷 163-168页

作者： Natthanej Luplertlop Department of Microbiology and Immunology Faculty of Tropical MedicineMahidol UniversityBangkokThailand

Dengue virus,one of the most important arthropod-borne viruses,infected to human can severely cause dengue hemorrhagic fever and dengue shock syndrome.There are expected about 50 million dengue infections and 500000 individuals are hospitalized with dengue hemorrhagic fever,mainly in Southeast Asia,Pacific,and in Americas reported each year.The rapid expansion of global dengue is one of a major public health challenge,together with not yet successful solutions of dengue epidemic control strategies.Thus,these dynamic dengue viral infections exhibited high demographic,societal,and public health infrastructure impacts on human.This review aimed to highlight the current understanding of dengue mosquito immune responses and role of mosquito salivary glands on dengue infection.These information may provide a valuable knowledge of disease pathogenesis,especially in mosquito vector and dengue virus interaction,which may help to control and prevent dengue distribution.

关键词： Dengue aedes Mosquito immune Dengue vectors

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Larvicidal and knockdown effects of some essential oils against Culex quinquefasciatusSay, aedes aegypti(L.) and Anopheles stephensi(Liston)

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Advances in Bioscience and Biotechnology 2012年第7期3卷 855-862页

作者： A. Manimaran M. Mary Jee Jee Cruz C. Muthu S. Vincent S. Ignacimuthu Department of Advanced Zoology and Biotechnology Loyola College Chennai India Department of Biological Sciences AVIT Vinayaka Missions University Payyanoor Campus Salem India Entomology Research Institute Loyola College Chennai India

Efficacy of 25 essential oils was screened against filarial vector, Culex quinquefasciatus, for their larvicidal and knockdown effects in a preliminary study. Of these, 8 oils viz. calamus oil, cinnamon oil, citronella oil, clove oil, eucalyptus oil, lemon oil, mentha oil and orange oil exhibited 100% larvicidal activity at 1000 ppm and 100% knockdown effect at 10% concentration. These 8 oils were screened further against Cx. Quinquefasciatus, aedes aegypti and Anopheles stephensi for their larvicial and knockdown effects at different concentrations. Mentha oil was the most promising against An. Stephensi and Ae. Aegypti recording LC50 and LC90 values of 39.74 and 115.67 ppm and 46.23 and 165.36 ppm, respectively for larvicidal activity. Calamus oil was the most effective against Cx. Quinquefasciatus with LC50 and LC90 values of 40.40, and 140.07 ppm, respectively for larvicidal activity. Orange oil showed the most potent knockdown effect with the KT50 and KT95 values of 27.44, 26.22 and 29.91 and 70.81, 65.33 and 68.57 min, against An. stephensi, Cx. Quinquefasciatus and Ae. Aegypti, respectively. The results clearly indicated that mentha oil and calamus oil were the most promising larvicides and orange oil had potent knockdown effect against the tested mosquito species. These oils could be used to develop a new formulation to control mosquitoes.

关键词： Larvicidal Knockdown Essential Oils aedes aegypti Anopheles stephensi Culex quinquefasciatus

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Bio-Synthesis of Silver Nanoparticles from Leaf Extract of Pongamia pinnataas an Effective Larvicide on Dengue Vector aedes albopictus(Skuse) (Diptera: Culicidae)

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Advances in Entomology 2014年第2期2卷 93-101页

作者： Bannoth Reddya Naik G. Swarna Gowreeswari Yuvo Singh R. Satyavathi S. S. Daravath P. Ramachandra Reddy Department of Botany Osmania University Hyderabad India Department of Zoology Division of Medical Entomology University College of Science Osmania University Hyderabad India School of Physics Hyderabad Central University Hyderabad India

The frequent cases of dengue incidences are leading cause of illness and death in urban areas and aedes albopictus (Skuse) is a primary vector for Dengue transmission in India. Synthesis of leaf mediated silver nanoparticles especially with Pongamia pinnata is a potential substitute for the existing organophosphorus insecticides like Tenophos, malathion and fenthion etc., for mosquito control programme. The nanoparticles were characterized by UV-visible absorption spectrum, X-Ray Diffraction (XRD), Fourier Transform Infrared (FTIR) and Transmission Electron Microscopy (TEM). Analysis of TEM showed that the synthesized silver nanoparticles are in spherical shape with average size of 20 nm. Further the XRD analysis confirms the nano-crystalline phase of silver with face centred cubic (FCC) crystal structure. GC-MS analysis elucidated the presence of two active ingredients, such as 9-Octadecenoic acid (Z) and n-hexadecanoic acid, which are the prominent substances considered as larvicide. Larvae were exposed to varying concentrations of plant extracts and synthesized silver nanoparticles for 24 hours. From the results, it is found that plant extracts showed moderate larvicidal effects but, the synthesized silver nanoparticles had found to be toxic to larvae at LC50 (0.25 ppm) and LC90 (1 ppm).

关键词： Pongamia pinnata aedes albopictus Silver Nanoparticle Dengue 9-Octadecenoic Acid (Z)

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Distribution patterns and relationship between elevation and the abundance of aedes aegyptiin Mojokerto city 2012

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Open Journal of Animal Sciences 2013年第4期3卷 11-16页

作者： Zulfaidah Penata Gama Nobukazu Nakagoshi Madaniatul Islamiyah Department of Biology Faculty of Science Brawijaya University Malang Indonesia Graduate School for International Development and Cooperation (IDEC) Hiroshima University Kagamiyama Japan Graduate School for International Development and Cooperation (IDEC) Hiroshima University Kagamiyama Japan Department of Biology Faculty of Science Brawijaya University Malang Indonesia

Mosquitoes are one of the insects that have an important role as vectors of disease agents. The diseases transmitted by mosquitoes are still public health problems in Indonesia especially in East Java Province, for example, Dengue Hemorrhagic Fever (DHF). DHF is a very alarming disease because the occurrence of this epidemic is no longer just confined to certain geographic locations. At present, Mojokerto is a district in East Java region known as endemic for DHF in Indonesia. The number of cases in this district tends to rise and expand distributed. The spread pattern of dengue cases in this district is not certainly known. The aim of this research is to determine the distribution pattern of mosquitoes and its relationship between elevation and density of aedes aegypti in the Mojokerto district that caused dengue virus transmission. Sampling was conducted at two locations in the Mojokerto district, in the urban and rural, namely Prajurit Kulon for urban and Dlanggu for rural. We used survey for mosquito larvae by WHO standard and for mosquito eggs by ovitrap. The coordinates of sampling locations are recorded using GPS and then identification of mosquitoes is performed at the Laboratory of Ecology and Animal Diversity, University of Brawijaya. Quantitative data were analyzed to determine the abundance, relative abundance, frequency, relative frequency and IVI (Importance Value Index). Mosquito distribution patterns were analyzed with Morisita index. The research findings indicated that there are five mosquitoes species consisted of aedes aegypti, aedes albopictus, aedes laniger, Culex bitaeniorchynchus and Culex quinquefasciatus. aedes aegypti is the dominant species in urban area while Culex quinquefasciatus is the dominant species in rural area. Morisita index showed that mosquito dispersal on patterns in Mojokerto district is uniform. The elevation of the area and density of aedes aegypti has a positive correlation.

关键词： aedes aegypti Distribution Pattern Elevation Mojokerto

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