检索结果-南通市图书馆

Isolation of S-adenosylmethionine Synthetase Gene from Suaeda salsa and Its Differential Expression Under NaCl Stress

引用

Acta Botanica Sinica 2003年第11期45卷 1359-1365页

作者：马秀灵王增兰戚元成赵彦修张慧山东师范大学生命科学学院逆境植物重点实验室济南250014

adomet plays numerous roles of being the major methyl-group donor in trans-methylation reactions. To gain insight into the possible functions of the adomet protein of Suaeda salsa L. in response to salt stress, S adenosylmethionine synthetase gene (SAMS2) was analyzed. We isolated SAMS2 cDNA clone (AF321001) from a lambda -Zap cDNA library constructed from the halophyte S. salsa Pall aerial tissue treated with 400 mmol/L NaCl. SsSAMS2 was found to encode a S-adenolyl-L-methionine synthetase enzyme (adomet synthetase). The fragment was 1 531 bp with an open reading frame of 395 amino acids, the calculated molecular weight was about 43 kD. SsSAMS2 showed the highest homology to SAMS2 gene of Catharanthus roseus G. Don., with 93% identity in deduced amino acid sequence. Southern blotting analysis showed that SsSAMS2 might be a two-copy gene in S. salsa genome. Northern blot indicated that the cDNA was up-regulated by salt and other stresses. Enzyme activity assay indicated that the activity of SAMS2 increased under NaCl stress.

关键词： adomet Suaeda salsa salt stress SAMS gene sequence expression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

S-adenosyl-L-methionine, trehalose and oleanolic acid in few plants

引用

Health 2010年第8期2卷 968-972页

作者： Dipankar Malakar Paramita Chaudhuri Trina Dutta Anil K Ghosh 不详

S-Adenosyl-L-Methionine (adomet), S-Adenosyl- L-Homocysteine (AdoHcy), adenosine, trehalose and oleanolic acid were measured in six medicinal herbs and three spices. The findings showed that adomet content was forty six fold higher in the leaves of Catharanthus roseus as compared with average adomet content of rest of the plants. In comparison to other plants, Withania somnifera had very high AdoHcy: Ado-Met and adenosine: adomet ratios indicating it may have contained high adomet. Trehalose was found to be twenty fold and nine fold higher in bulb of Allium cepa and root of Withania somnifera respectively with respect to average trehalose content of rest of the plants. Ocimum sanctum appeared to be a rich source of oleanolic acid. It appeared from our study that Catharanthus roseus, Allium cepa and Ocimum sanctum could be utilized as natural sources of adomet, trehalose and oleanolic acid respectively.

关键词： adomet AdoHcy Adenosine Trehalose Oleanolic Acid

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：