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Chemically induced hypoxia by dimethyloxalylglycine(DMOG)-loaded nanoporous silica nanoparticles supports endothelial tube formation by sustained VEGF release from adipose tissue-derived stem cells

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Regenerative Biomaterials 2021年第5期8卷 63-74页

作者： Sarah Zippusch Karen FWBesecke Florian Helms Melanie Klingenberg Anne Lyons Peter Behrens Axel Haverich Mathias Wilhelmi Nina Ehlert Ulrike Boer Lower Saxony Centre for Biomedical Engineering Implant Research and Development(NIFE)Hannover Medical SchoolStadtfelddamm 3430625 HannoverGermany Division for Cardiac Thoracic-Transplantation-and Vascular SurgeryHannover Medical SchoolCarl-Neuberg-Straße 130625 HannoverGermany Institute of Inorganic Chemistry Leibniz University HannoverCallinstraße 930167 HannoverGermany Cluster of Excellence Hearing4all Carl-von-Ossietzky-Straße 9-1126129 OldenburgGermany Department of Vascular-and Endovascular Surgery St.Bernward HospitalTreibestraße 931134 HildesheimGermany

Inadequate vascularization leading to insufficient oxygen and nutrient supply in deeper layers of bioartificial tissues remains a limitation in current tissue engineering approaches to which prevascularization offers a promising *** triggering pre-vascularization by enhanced vascular endothelial growth factor(VEGF)expression can be induced chemically by dimethyloxalylglycine(DMOG).Nanoporous silica nanoparticles(NPSNPs,or mesoporous silica nanoparticles,MSNs)enable sustained delivery of molecules and potentially release DMOG allowing a durable capillarization of a *** we evaluated the effects of soluble DMOG and DMOG-loaded NPSNPs on VEGF secretion of adipose tissue-derived stem cells(ASC)and on tube formation by human umbilical vein endothelial cells(HUVEC)-ASC *** doses of 100 mM and 500 mM soluble DMOG on ASC resulted in 3-to 7-fold increased VEGF levels on day 9(Ptissue-engineered *** studies will evaluate their effect in hydrogels under perfusion.

关键词： pre-vascularization tissue engineering adipose tissue-derived stem cells dimethyloxalylglycine nanoporous silica nanoparticles

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Over-expression of VEGF165 in the adipose tissue-derived stem cells via the lentiviral vector

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Chinese Medical Journal 2011年第19期124卷 3093-3097页

作者： SUN Xiang-zhou LIU Gui-hua WANG Zhuo-qing ZHENG Fu-fu BIAN Jun HUANG Yan-ping GAO Yong ZHANG Ya-dong DENG Chun-hua Department of Urology First Affiliated Hospital of Sun Yet-sen University Guangzhou Guangdong 510080 China Department of Scientific Research First Affiliated Hospital of Sun Yet-sen University Guangzhou Guangdong 510080 China

Background Many researchers studied the possibility of using stem cells as gene therapeutic vector. But few related reports on the adipose tissue-derived stem cells （ADSCs） are available. Therefore we intended to construct a lentiviral VEGF165 expression vector and then infect the ADSCs to produce therapeutic seed *** EHS1001-68950485313912 clone was mutated by PCR method to produce consensus fragment of VEGF165 transcript （NM_001025368）. Lentivirus was enveloped with pGC-FU, pHelper 1.0 and pHelper 2.0 plasmids in 293T *** then the ADSCs （multiplicity of infection=20） were transfected with the vectors after titer determination. Stable expression of VEGF165 in ADSCs was confirmed by immunofluorescence staining, enzyme-linked immunosorbent assay （ELISA） and Western blotting *** DNA sequencing and 293T transfection verified VEGF165 was linked to the GFP fused vector. The virus titer is up to 2x10a determined by quantitative PCR. VEGF165 transduced cells could show green fluorescence confirmed by immunofluorescence staining （almost 95%）. ELISA analyses could detect out the density of VEGF was 850.86-1202.13pg/ml （mean （923.00±31.22） pg/ml） in the supernatant of VEGF16s-transduced cells but not detected in the GFP-transduced cells （P 〈0.001） and the Western blotting analyses also confirmed VEGF165 expression in VEGF165-transduced *** The VEGF165 over-expression ADSCs were obtained and may be used as a cell therapeutic tool and may be applied for vascular regeneration, especially in the treatment of erectile dysfunction.

关键词： adipose tissue-derived stem cells VEGF16s lentivirus over-expression

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High-Fat Diets-Induced Metabolic Alterations Alter the Differentiation Potential of adipose tissue-derived stem cells

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Open Journal of Endocrine and Metabolic Diseases 2013年第3期3卷 197-207页

作者： Vikie Lamontagne Souhad El Akoum Isabelle Cloutier Jean-Francois Tanguay Département des Sciences Biomédicales Universitéde MontréalMontréalCanada Montreal Heart Institute MontréalCanada

Background: adipose tissue-derived stem cells (ASC) possess the ability to differentiate into adipocytes or endothelial cells to help in the adipogenesis, vasculogenesis and vascular repair. This study aims at determining the impact of high-fat diets (HFD)-induced type 2 diabetes (T2D) on the differentiation potential of ASC. Results: C57BL/6J male mice were fed a vegetal (VD) or an animal (AD) HFD. Isolation of ACS from mice showing different levels of metabolic alterations reveals that advanced T2D did not affect the number of cells per gram of tissue. Rather, a higher proportion of inflammatory CD36+ cells was identified in HFD fed mice. Despite a marked decreased expression of adipogenic genes (aP2, C/EBPα and PPARγ2), ASC from HFD groups had a higher adipogenic potential and a lower endothelial differentiation potential in vitro compared to control. ASC from the VD group had enhanced cyclin B1 expression and had more adipogenic potential compared to AD group. Conclusion: Our results demonstrate that the metabolic modifications, linked to the nature of fatty acids in diets, modulate the differentiation potential of ASC with increased adipogenesis to the detriment of the endothelial pathway. Results highlight the importance of evaluating the ASC differentiation behavior in a context of autologous cell-based therapy for the repair of vascular tissues in diabetic patients.

关键词： adipose tissue-derived stem cells Type 2 Diabetes Adipocytes Endothelial cells Differentiation Vascular Cellular Therapy

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Optimization of adipose tissue-derived mesenchymal stromal cells transplantation for bone marrow repopulation following irradiation

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World Journal of stem cells 2022年第3期14卷 245-263页

作者： Min-Jung Kim Won Moon Jeonghoon Heo Sangwook Lim Seung-Hyun Lee Jee-Yeong Jeong Sang Joon Lee Department of Biochemistry Cancer Research Institute Kosin University College of MedicineSeo-gu 49267BusanSouth Korea Department of Internal Medicine Kosin University College of MedicineSeo-gu 49267BusanSouth Korea Department of Molecular Biology and Immunology Kosin University College of MedicineSeo-gu 49267BusanSouth Korea Department of Radiation Oncology Kosin University College of MedicineSeo-gu 49267BusanSouth Korea Department of General Surgery Kosin University College of MedicineSeogu 49267BusanSouth Korea Department of Ophthalmology Gospel HospitalKosin University College of MedicineSeo-gu 49267BusanSouth Korea

BACKGROUND Bone marrow(BM)suppression is one of the most common side effects of radiotherapy and the primary cause of death following exposure to *** concerted efforts,there is no definitive treatment method *** studies have reported using mesenchymal stromal cells(MSCs),but their therapeutic effects are *** We administered and examined the effects of various amounts of adipose-derived MSCs(ADSCs)in mice with radiation-induced BM *** Mice were divided into three groups:Normal control group,irradiated(RT)group,and stem cell-treated group following whole-body irradiation(WBI).Mouse ADSCs(mADSCs)were transplanted into the peritoneal cavity either once or three times at 5×10^(5) cells/200μ*** white blood cell count and the levels of,plasma cytokines,BM mRNA,and BM surface markers were compared between the three *** BM-derived CD34+hematopoietic progenitor cells were co-cultured with human ADSCs(hADSCs)or incubated in the presence of hADSCs conditioned media to investigate the effect on human cells in *** The survival rate of mice that received one transplant of mADSCs was higher than that of mice that received three *** transplantations of ADSCs delayed the repopulation of BM hematopoietic stem ***-inflammatory effects and M2 polarization by intraperitoneal ADSCs might suppress erythropoiesis and induce myelopoiesis in sub-lethally RT *** The results suggested that an optimal amount of MSCs could improve survival rates post-WBI.

关键词： adipose tissue-derived stem cells Bone marrow suppression Mesenchymal stromal cells Radiation Cell therapy

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Recent advances in andrology-related stem cell research

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Asian Journal of Andrology 2008年第2期10卷 171-175页

作者： Ching-Shwun Lin Zhong-Cheng Xin Chun-Hua Deng Hongxiu Ning Guiting Lin Tom F. Lue Knuppe Molecular Urology Laboratory Department of Urology School of Medicine University of California San Francisco CA 94143 USA Andrology Center Peking University First Hospital Beijing 100009 China Urology Department The First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510080 China

stem cells hold great promise for regenerative medicine because of their ability to self-renew and to differentiate into various cell types. Although embryonic stem cells （BSC） have greater differentiation potential than adult stem cells, the former is lagging in reaching clinical applications because of ethical concerns and governmental restrictions. Bone marrow stem cells （BMSC） are the best-studied adult stem cells （ASC） and have the potential to treat a wide variety of diseases, including erectile dysfunction （ED） and male infertility. More recently discovered adipose tissuederived stem cells （ADSC） are virtually identical to bone marrow stem cells in differentiation and therapeutic potential, but are easier and safer to obtain, can be harvested in larger quantities, and have the associated benefit of reducing obesity. Therefore, ADSC appear to be a better choice for future clinical applications. We have previously shown that ESC could restore the erectile function of neurogenic ED in rats, and we now have evidence that ADSC could do so as well. We are also investigating whether ADSC can differentiate into Leydig, Sertoli and male germ cells. The eventual goal is to use ADSC to treat male infertility and testosterone deficiency. （Asian JAndrol 2008 Mar; 10： 171-175）

关键词： stem cells bone marrow stem cells adipose tissue-derived stem cells erectile dysfunction male infertility

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stem cells： novel players in the treatment of erectile dysfunction

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Asian Journal of Andrology 2012年第1期14卷 145-155,179页

作者： Haiyang Zhang Maarten Albersen Xunbo Jin Guiting Lin Minimally Invasive Urology Center Provincial Hospital Affiliated to Shandong University Jinan 250021 China Knuppe Molecular Urology Laboratory Department of Urology School of Medicine University of California San Francisco CA 94143 USA Laboratory of Experimental Urology Department of Urology University Hospitals Leuven Leuven 3000 Belgium

stem cells are defined by their capacity for both self-renewal and directed differentiation; thus, they represent great promise for regenerative medicine. Historically, stem cells have been categorized as either embryonic stem cells （ESCs） or adult stem cells （ASCs）. It was previously believed that only ESCs hold the ability to differentiate into any cell type, whereas ASCs have the capacity to give rise only to cells of a given germ layer. More recently, however, numerous studies demonstrated the ability of ASCs to differentiate into cell types beyond their tissue origin. The aim of this review was to summarize contemporary evidence regarding stem cell availability, differentiation, and more specifically, the potential of these cells in the diagnosis and treatment of erectile dysfunction （ED） in both animal models and human research. We performed a search on PubMed for articles related to definition, iocalisation and circulation of stem cells as well as the application of stem cells in both diagnosis and treatment of ED. Strong evidence supports the concept that stem cell therapy is potentially the next therapeutic approach for ED. To date, a large spectrum of stem cells, including bone marrow mesenchymal stem cells, adipose tissue-derived stem cells and muscle-derived stem cells, have been investigated for neural, vascular, endothelial or smooth muscle regeneration in animal models for ED. In addition, several subtypes of ASCs are localized in the penis, and circulating endogenous stem cells can be employed to predict the outcome of ED and ED-related cardiovascular diseases.

关键词： adipose tissue-derived stem cells bone marrow stem cells erectile dysfunction mesenchymal stem cells stem cells

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Current Status of ADSCs-Enriched Fat Grafts in Plastic Surgery

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Chinese Journal of Plastic and Reconstructive Surgery 2020年第4期2卷 263-276页

作者： Yating YIN Jianhua LI Hao WANG Pingping WANG Wanling ZHENG Qiang LI Peisheng JIN Department of Plastic Surgery Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu 221002China Department of Burn Surgery Changhai HospitalNaval Medical UniversityShanghai 200433China Department of Plastic Surgery The First People’s Hospital of XuzhouXuzhouJiangsu 221002China Department of Plastic Surgery Zibo Women&Children HospitalZiboShandong 255029China Department of Burn Surgery The First People’s Hospital of XuzhouXuzhouJiangsu 221002China

Autologous fat grafting is an increasingly popular technique in plastic surgery for volume augmentation and ***,the unpredictability of long-term volume retention limits its clinical *** animal studies have documented the positive effects of adipose tissue-derived stem cells(ADSCs)on the acceleration of ***,the results have been inconsistent,and there is an insufficient number of high-quality clinical studies to formulate evidence-based recommendations for ADSC-enriched fat ***,related technical standards,such as the final count of harvested ADSCs and the enrichment ratio,have not yet been *** systematic review included all clinical trials on ADSC-enriched fat grafts in plastic surgery from PubMed in the past 10 years,as well as all registered clinical trials on *** examine the current landscape of ADSCs harvest,we summarize the current applications of ADSCs in the field of plastic surgery and discuss the current barriers to universal clinical use.

关键词： adipose tissue-derived stem cells Stromal vascular fraction Fat graft Cell-assisted lipotransfer Plastic surgery

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