检索结果-南通市图书馆

Effects of various antimicrobial agents on multi-directional differentiation potential of bone marrow-derived mesenchymal stem cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2019年第6期11卷 322-336页

作者： Hui Li Bing Yue Department of Bone and Joint Surgery Renji Hospital Shanghai Jiaotong University School of Medicine

Antimicrobial drugs of several classes play an important role in the treatment of bone and joint infections. In addition to fighting pathogenic microorganisms, the effects of drugs on local tissues and cells are also related to the course and prognosis of bone and joint infections. The multi-directional differentiation potential of bone marrow-derived mesenchymal stem cells (MSCs) is essential for tissue repair after local injury, which is directly related to the recovery of bone, cartilage, and medullary adipose tissue. Our previous studies and the literature indicate that certain antimicrobial agents can regulate the differentiation potential of bone marrow-derived MSCs. Here, in order to systematically analyze the effects of various antimicrobial drugs on local tissue regeneration, we comprehensively review the studies on the effects of these drugs on MSC differentiation, and classify them according to the three differentiation directions (osteogenesis, chondrogenesis, and adipogenesis). Our review demonstrates the specific effects of different antimicrobial agents on bone marrow-derived MSCs and the range of concentrations at which they work, and provides a basis for drug selection at different sites of infection.

关键词： Antimicrobial agents Bone marrow mesenchymal stem cells Osteogenesis Chondrogenesis adipogenesis

The thermogenic circuit: Regulators of thermogenic competency and differentiation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2015年第2期2卷 164-172页

作者： Matthew D.Lynes Yu-Hua Tseng Section on Integrative Physiology and Metabolism Joslin Diabetes CenterHarvard Medical SchoolBostonMAUSA

In mammals,a thermogenic mechanism exists that increases heat production and consumes energy.Recent work has shed light on the cellular and physiological mechanisms that control this thermogenic circuit.Thermogenically active adipocytes,namely brown and closely related beige adipocytes,differentiate from progenitor cells that commit to the thermogenic lineage but can arise from different cellular origins.Thermogenic differentiation shares some features with general adipogenesis,highlighting the critical role that common transcription factors may play in progenitors with divergent fates.However,thermogenic differentiation is also discrete from the common adipogenic program and,excitingly,cells with distinct origins possess thermogenic competency that allows them to differentiate into thermogenically active mature adipocytes.An understanding of this thermogenic differentiation program and the factors that can activate it has led to the development of assays that are able to measure thermogenic activity both indirectly and directly.By combining these assays with appropriate cell models,novel therapeutic approaches to combat obesity and its related metabolic disorders by enhancing the thermogenic circuit can be developed.

关键词： adipogenesis Autologous transplants Brown adipose tissue Cell therapy UCP1 Obesity Preadipocyte Thermogenesis

Pharmacotherapy of Diabetes Mellitus Type 2: A Review on Various Hypoglycemics

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Clinical and Nursing Research 2022年第5期6卷 57-67页

作者： Muhammad Khalid Khan Rizwan U Baig Drug Delivery and Cosmetics Lab(DDCL) Faculty of PharmacyGomal UniversityDera Ismail Khan29050Pakistan Department of Pharmacy Quaid-i-Azam University IslamabadIslamabad 44000Pakistan

Diabetes is a cosmopolitan medical issue ramifying a large population regardless of ethnicity.Elevated glycemic condition in a patient is explained as diabetes.It can be mainly categorized into Non-Insulin Dependent Diabetes Mellitus(NIDDM)and Insulin Dependent Diabetes Mellitus(IDDM)or Type 1 or Type 2 respectively.Type 1 or IDDM needs insulin therapy exclusively while Type 2 can be controlled via various hypoglycemic agents.Conventional classes contain glitazones,biguanides,sulfonylurea’s and soon,while newly developed drug classes include gliptins or DPP-4 inhibitors,SGLT-2 receptor inhibitors and GLP-1 receptor agonists.The novelty in the anti-hyperglycemic agents have given promising results as compared to the conventional drugs.Insulin cannot be negated as far as its glycemic control is concerned.Furthermore,development of Insulin analogues like detemir,glargine,Lisprohave been shown to control glucose levels in the plasma more efficiently.

关键词： Pre-prandial Postprandial Ketoacidosis adipogenesis Oral Hypoglycemic Drugs(OHD)

Comparative characterization and osteogenic/adipogenic differentiation of mesenchymal stem cells derived from male rat hair follicles and bone marrow

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cell Regeneration 2020年第1期9卷 130-143页

作者： Abdel Kader A.Zaki Tariq I.Almundarij Faten A.M.Abo-Aziza Department of Veterinary Medicine College of Agriculture and Veterinary MedicineQassim UniversityBuraydahSaudi Arabia Department of Physiology Faculty of Veterinary MedicineCairo UniversityGizaEgypt Department of Parasitology and Animal Diseases Veterinary Research DivisionNational Research CentreCairoEgypt

Clinical applications of cell therapy and tissue regeneration under different conditions need a multiplicity of adult stem cell sources.Up to date,little is available on the comparative isolation,characterization,proliferation,rapid amplification,and osteogenic/adipogenic differentiation of rat mesenchymal stem cells(MSCs)isolated from living bulge cells of the hair follicle(HF)and bone marrow(BM)from the same animal.This work hopes to use HF-MSCs as an additional adult stem cell source for research and application.After reaching 80%confluence,the cell counting,viability%,and yields of HF-MSCs and BM-MSCs were nearly similar.The viability%was 91.41±2.98 and 93.11±3.06 while the cells yield of initial seeding was 33.15±2.76 and 34.22±3.99 and of second passage was 28.76±1.01 and 29.56±3.11 for HF-MSCs and BM-MSCs respectively.Clusters of differentiation(CDs)analysis revealed that HF-MSCs were positively expressed CD34,CD73 and CD200 and negatively expressed CD45.BM-MSCs were positively expressed CD73 and CD200 and negatively expressed of CD34 and CD45.The proliferation of HFMSCs and BM-MSCs was determined by means of incorporation of Brd-U,population doubling time(PDT)assays and the quantity of formazan release.The percentage of Brd-U positive cells and PDT were relatively similar in both types of cells.The proliferation,as expressed by the quantity of formazan assay in confluent cells,revealed that the quantity of release by BM-MSCs was slightly higher than HF-MSCs.Adipogenic differentiated BM-MSCs showed moderate accumulation of oil red-O stained lipid droplets when compared to that of HF-MSCs which exhibited high stain.The total lipid concentration was significantly higher in adipogenic differentiated HF-MSCs than BMMSCs(P<0.05).It was found that activity of bone alkaline phosphatase and calcium concentration were significantly higher(P<0.01 and P<0.05 respectively)in osteogenic differentiated BM-MSCs than that of HF-MSCs.The present findings demonstrate that the HF-MSCs are very similar in most tested characte

关键词： Stem cell Bone marrow Hair follicle Osteogenesis adipogenesis

Hepatic lipid homeostasis by peroxisome proliferator-activated receptor gamma 2

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Liver Research 2018年第4期2卷 209-215页

作者： Yoon Kwang Lee Jung Eun Park Mikang Lee James P.Hardwick Department of Integrative Medical Sciences College of MedicineNortheast Ohio Medical UniversityRootstownOHUSA Department of Food Science and Human Nutrition Chonbuk National UniversityDeokjin-guJeonjuRepublic of Korea

Peroxisome proliferator-activated receptor gamma(PPARγor PPARG)is a ligand-activated transcription factor belonging to the nuclear hormone receptor superfamily.It plays a master role in the differentiation and proliferation of adipose tissues.It has two major isoforms,PPARγ1 and PPARγ2,encoded from a single gene using two separate promoters and alternative splicing.Among them,PPARγ2 is most abundantly expressed in adipocytes and plays major adipogenic and lipogenic roles in the tissue.Furthermore,it has been shown that PPARγ2 is also expressed in the liver,specifically in hepatocytes,and its expression level positively correlates with fat accumulation induced by pathological conditions such as obesity and diabetes.Knockout of the hepatic Pparg gene ameliorates hepatic steatosis induced by diet or genetic manipulations.Transcriptional activation of Pparg in the liver induces the adipogenic program to store fatty acids in lipid droplets as observed in adipocytes.Understanding how the hepatic Pparg gene expression is regulated will help develop preventative and therapeutic treatments for non-alcoholic fatty liver disease(NAFLD).Due to the potential adverse effect of hepatic Pparg gene deletion on peripheral tissue functions,therapeutic interventions that target PPAR g for fatty liver diseases require fine-tuning of this gene's expression and transcriptional activity。

关键词： Non-alcoholic fatty liver disease(NAFLD) High fat diet(HFD) adipogenesis Gene expression Peroxisome proliferator-activated receptor gamma(PPARγ)

Freely Available Tool(FAT)for automated quantification of lipid droplets in stained cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2017年第2期41卷 55-58页

作者： DIEGO MASONE ALDANA DGOJANOVICH YESICA R.FRONTINI-LOPEZ SAMANTA DEL VELIZ MARINA UHART DIEGO M.BUSTOS Laboratorio de Integración de Senales Celulares.IHEM-CONICET Universidad Nacional de Cuyo MendozaArgentina Facultad de Ingeniería Universidad Nacional de CuyoMendozaArgentina Facultad de Ciencias Exactas y Naturales Universidad Nacional de CuyoMendozaArgentina

In this study,we propose an automatic procedure for digital image processing.We describe a method that can efficiently quantify and characterize lipid droplets distributions in different cell types in culture.Prospectively,the lipid droplets detection method described in this work could be applied to static or time-lapse data,collected with a simple visible light or fluorescence microscopy equipment.Fully automated algorithms were implemented in Octave,a freely available scientific package.

关键词： adipogenesis 3T3-L1 cells human stem cells automatic image processing

Hyperglycemia Increases adipogenesis andlnhibits Osteogenic Differentiation in MG-63 Cell

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hyperglycemia Increases Adipogenesis andlnhibits Osteogenic ...

Regulation of Orbital Fibrosis and adipogenesis by Pathogenic Th17 Cells in Graves' Orbitopathy

第二届中国医学细胞生物学学术大会暨细胞生物学教学改革会...

作者： Weiwei Wang Xiaolin Zhang Jianhong Yang College of Life Science,Graduate University of Chinese Academy of Sciences,19 Yuquan Road,Beijing 100049

It is well known that insulin dependent diabetes mellitus(IDDM),marked by high blood glucose level and no insulin secretion,is associated with increased risk of osteopenia and osteoprosis.However,the underlying mechan... 详细信息

关键词： osteoporosis hyperglycemia adipogenesis ERK-1/2 phosphorylation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Regulation of Orbital Fibrosis and Adipogenesis by Pathogeni...

C/EBPβ-regulated Autophagy Facilitates adipogenesis

第十三届全国免疫学学术大会

作者： Sijie Fang Yazhuo Huang Sisi Zhong Xingtong Liu Bin Li Huifang Zhou Xianqun Fan Shanghai Ninth People's Hospital Shanghai JiaoTong University School of Medicine Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology Shanghai Institute of Immunology Shanghai JiaoTong University School of Medicine Department of Immunology and Microbiology Shanghai JiaoTong University School of Medicine

Purpose: To study the phenotype of Th17 cells in patients withGOand healthy subjects, investigate the fibrosis and adipogenesis in orbital fibroblasts(OFs) modulated by interleukin(IL)-17A, and determine the interaction between Th17 cells and OFs.Methods: Blood samples and orbital tissues from GO patients and healthy controls were collected.We conducted multicolor flow cytometry, immunohistochemical and immunofluorescent stainings, Western blotting, a PathScan intracellular signaling assay, Luminex and enzyme-linked immunosorbent assays, and protein mass spectrum.Results: Interferon-γ– and IL-22–expressing Th17 cells are increased in GO patients, which are positively related to clinical activity score.Costimulatory molecules are highly expressed in GO orbits and most GO OFs are CD90.IL-17A promotes TGF-β–induced fibrosis in CD90 OFs but impedes 15-deoxy-Δ-prostaglandin J–induced adipogenesis in CD90OFs.Th17 cells promote proinflammatory cytokine secretion in both CD90 and CD90OFs.Meanwhile, both CD90 and CD90OFs contribute to Th17 cell differentiation through prostaglandin E production, which can be attenuated by indomethacin.Furthermore, Th17 cells upregulate costimulatory molecule expression on OFs.Conclusion: Our findings unravel the pathogenicity of IL-17A in the initiation and progression of GO.In-depth interpretation of the molecular basis of OFs delineated by CD90 and Th17–OF interaction will help to afford a novel approach to better therapeutic strategies for GO.

关键词： Graves’ orbitopathy IL-17A Fibrosis adipogenesis

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

C/EBPβ-regulated Autophagy Facilitates Adipogenesis