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Stereotaxic microinjection of adenovirus-mediated human tissue Kallikrein gene reduces apoptosis in a rat model of middle cerebral artery occlusion

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Neural Regeneration Research 2008年第8期3卷 847-852页

作者： Ruiyan Lu Lianhong Yang Qingyu Shen Mei Li Xiangpen Li Ying Peng Department of Neurology Second Affiliated Hospital of Sun Yat-sen University Guangzhou 510120 Guangdong Province China

BACKGROUND：Several studies have demonstrated that Kallikrein gene transfer provides neuroprotection. Whether the neuroprotective effects of human tissue Kallikrein （HTK） are associated with apoptosis remains unclear. OBJECTIVE： To investigate the effects of HTK on apoptosis in the peripheral cerebral infarct region. DESIGN, TIME AND SETTING： The completely randomized grouping, gene engineered, controlled experiment was performed at the Lin Baixin Laboratory Center, the Second Affiliated Hospital of Sun Yat-sun University between September 2007 and April 2008. MATERIALS： Ninety clean, healthy, male, Sprague Dawley rats were included. pUC19-HTK plasmid was constructed in the Laboratory for Neurology, the Second Affiliated Hospital of Sun Yat-sen University, China. bcl-2, bax, caspase-3, and β-actin were designed and purified by Shanghai Shuiyuan Company, China. METHODS： Middle cerebral artery occlusion （MCAO） model was established in all rats. At 72 hours after MCAO model establishment, rats were randomly divided into 3 groups, with 30 rats per group： blank control, saline, and pAdCMV-HTK. The saline and pAdCMV-HTK groups were respectively stereotactically micro-injected with 5 μL physiological saline or pAdCMV-HTK at the area surrounding the cerebral infarction region. Only puncture was performed, without any injection, in the blank control group. MAIN OUTCOME MEASURES： At 72 hours after MCAO establishment, as well as at 24 hours, 72 hours, and 7 days subsequent to treatment, exogenous HTK expression was detected by immunohistochemistry. Apoptosis was examined by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling （TUNEL）, while mRNA levels of bcl-2, bax, and caspase-3 were detected by reverse transcription-polymerase chain reaction. In addition, neurological severity scores were evaluated prior to and after treatments. RESULTS： Ninety rats were included in the final analysis. HTK was primarily detected in the cytoplasm at 24 hours after pAdCMV-HTK injection. Thereafter, HTK expression gradually

关键词： adenovirus-mediated apoptosis apoptotic factor cerebral infarction kallikrein

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adenovirus-mediated corneal endotheliitis: a case report

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International Journal of Ophthalmology(English edition) 2019年第10期12卷 1659-1661页

作者： Shuang-Yong Wang Bei Li Dong-Hao Li Ying Tian The Third Affiliated Hospital Guangzhou Medical University

Dear Editor,Cornealendotheliitisisacommonandintriguing clinicalentitycharacterizedbycornealedema,keratic precipitates,and mild to moderate anterior chamber reaction,which occupies the important pathogenic factor of corneal blindness[1].Robin et al[2]first described a patient who suffered from intraocular inflammation and progressive corneal endotheliitis associated with herpes simplex infection.Since then,accumulating clinical evidence confirmed that its etiology was mainly attributed to the family of herpesviridae,including herpes simplex virus,varicella zoster virus,and cytomegalovirus,which initiated the direct cell damage and immune-and inflammatory-mediated lesion on endothelial cells[1,3].

关键词： adenovirus-mediated corneal endotheliitis a case report

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Synergistic anticancer effect of exogenous wild-type p53 gene combined with 5-FU in human colon cancer resistant to 5-FU in vivo

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World Journal of Gastroenterology 2016年第32期22卷 7342-7352页

作者： Qi Xie Min-Yi Wu Ding-Xuan Zhang Yi-Ming Yang Bao-Shuai Wang Jing Zhang Jin Xu Wei-De Zhong Jia-ni Hu Medical Imaging Department Nansha Central Hospital Guangzhou First People's Hospital Guangzhou Medical University Guangzhou 511457 Guangdong Province China Department of Pathology Cancer Center SunYat-sen University Guangzhou 510080 Guangdong Province China Department of Pathology Guangzhou First People's Hospital Guangzhou Medical University Guangzhou 510180 Guangdong Province China Clinical Molecular Medicine and Molecular Diagnostics Laboratory Guangzhou First People's Hospital Guangzhou Medical University Guangzhou 510180 Guangdong Province China Karmanos Cancer Institute Wayne State University Detroit MI 48201 United States

AIM To investigate the anticancer effect of a recombinant adenovirus-mediated p53(r Ad-p53) combined with 5-fluorouracil(5-FU) in human colon cancer resistant to 5-FU in vivo and the mechanism of r Ad-p53 in reversal of 5-FU resistance.METHODS nude mice bearing human colon cancer SW480/5-FU(5-FU resistant) were randomly assigned to four groups(n = 25 each): control group, 5-FU group, r Ad-p53 group, and r Ad-p53 + 5-FU group. At 24 h, 48 h, 72 h, 120 h and 168 h after treatment, 5 mice were randomly selected from each group and sacrificed using an overdose of anesthetics. The tumors were removed and the protein expressions of p53, protein kinase C(PKC), permeability-glycoprotein(P-gp) and multidrug resistance-associated protein 1(MRP1)(Western blot) and apoptosis(TUNEL) were determined.RESULTS The area ratios of tumor cell apoptosis were larger in the r Ad/p53 + 5-FU group than that in the control, 5-FU and r Ad/p53 groups(P < 0.05), and were larger in the r Ad/p53 group than that of the control group(P < 0.05) and the 5-FU group at more than 48 h(P < 0.05). The p53 expression was higher in the r Ad/p53 and the r Ad/p53 + 5-FU groups than that of the control and 5-FU groups(P < 0.05), and were higher in the r Ad/p53 + 5-FU group than that of the r Ad/p53 group(P < 0.05). Overexpression of PKC, P-gp and MRP1 was observed in the 5-FU and control groups. In the r Ad/p53 + 5-FU group, the expression of P-gp and MRP1 was lower that of the control and 5-FU groups(P < 0.05), and the expression of PKC was lower than that of the control, 5-FU and r Ad/p53 groups at more than 48 h(P < 0.05). In the r Ad/p53 group, the expression of P-gp and MRP1 was lower that of the control and 5-FU groups at more than 48 h(P < 0.05), and the expression of PKC was lower than that of the control and 5-FU groups at more than 120 h(P < 0.05).CONCLUSION5-FU combined with r Ad-p53 has a synergistic anticancer effect in SW480/5-FU(5-FU resistance), which contributes to reversal of 5-FU resistance.

关键词： Human colon cancer Multidrug resistance 5-Fluorouracil Recombinant adenovirus-mediated p53 Xenografts in nude mice

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