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achyranthes bidentata polypeptides prevent apoptosis by inhibiting the glutamate current in cultured hippocampal neurons

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Neural Regeneration Research 2020年第6期15卷 1086-1093页

作者： Rong-Lu Pan Wen-Qing Hu Jie Pan Li Huang Cheng-Cheng Luan Hong-Mei Shen Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Institute of Nautical Medicine Co-Innovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina Department of Neurobiology Physiology and BehaviorCollege of Biological ScienceDavisCAUSA Affiliated Mental Health Center of Nantong University Nantong Brain HospitalNantongJiangsu ProvinceChina

Glutamate-induced excitotoxicity plays a critical role in the neurological impairment caused by middle cerebral artery *** bidentata polypeptides have been shown to protect against neurological functional damage caused by middle cerebral artery occlusion,but the underlying neuroprotective mechanisms and the relationship to glutamate-induced excitotoxicity remain ***,in the current study,we investigated the protective effects of achyranthes bidentata polypeptides against glutamate-induced excitotoxicity in cultured hippocampal *** neurons were treated with Mg^2+-free extracellular solution containing glutamate(300μM)for 3 hours as a model of glutamate-mediated excitotoxicity(glutamate group).In the normal group,hippocampal neurons were incubated in Mg^2+-free extracellular *** the achyranthes bidentata polypeptide group,hippocampal neurons were incubated in Mg^2+-free extracellular solution containing glutamate(300μM)and achyranthes bidentata polypeptide at different *** 24 hours after exposure to the agents,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and Hoechst 33258 staining were used to assess neuronal viability and nuclear m'orphology,***-3 expression and activity were evaluated using western blot assay and colorimetric enzymatic assay,*** various time points after glutamate treatment,reactive oxygen species in cells were detected by H2 DCF-DA,and mitochondrial membrane potential was detected by rhodamine 123 *** examine the effect of achyranthes bidentata polypeptides on glutamate receptors,electrophysiological recording was used to measure the glutamate-induced inward current in cultured hippocampal *** bidentata polypeptide decreased the percentage of apoptotic cells and reduced the changes in caspase-3 expression and activity induced by *** addition,achyranthes bidentata polypeptide attenuated the amplitud

关键词： achyranthes bidentata polypeptides apoptosis caspase-3 excitotoxicity glutamate receptors mitochondrial dysfunction mitochondrial membrane potential neuroprotection reactive oxygen species staurosporine

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Tissue-based metabolite profiling and qualitative comparison of two species of achyranthes roots by use of UHPLC-QTOF MS and laser micro-dissection

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Journal of Pharmaceutical Analysis 2018年第1期8卷 10-19页

作者： Yogini Jaiswal Zhitao Liang Alan Ho Hubiao Chen Leonard Williams Zhongzhen Zhao Center for Excellence in Post-Harvest Technologies The North Carolina Research Campus School of Chinese Medicine Hong Kong Baptist University

achyranthes bidentata and achyranthes aspera are saponin and steroid rich medicinal plants, used extensively for therapeutic treatments in Traditional Chinese Medicine(TCM) and Ayurveda. A. bidentata is reported to be one of the rare and extensively exploited medicinal plant species that face the issue of being *** qualitative substitute with identical phyto-constituents contributing to similar composition and pharmacological benefits will help in reducing the burden of exploitation of the natural habitats of such *** the present study, a comparative metabolite analysis of the whole drug and specific tissues isolated by laser micro-dissection(LMD) was carried out for both the selected species, by use of ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-QTOF MS). The results of the study indicate that the cortex and the medullary ray tissues are rich in their content of steroidal and saponin constituents such as(25 S)-inokosterone-20,22-acetonide, ginsenoside Ro, bidentatoside II and achyranthoside *** profiling of the whole tissues of both the species indicates presence of identical constituents. Thus,it is inferred that A. bidentata and A. aspera can be used as qualitative substitutes for each other.

关键词： achyranthes aspera achyranthes bidentata Steroids Glycosides Saponins

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One-pot synthesis of silver nanocomposites from achyranthes aspera:An eco-friendly larvicide against Aedes aegypti L.

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Asian Pacific Journal of Tropical Biomedicine 2020年第2期10卷 54-64页

作者： Aarti Sharma Pushplata Tripathi Sarita Kumar Department of Life Sciences(SOS) Indira Gandhi National Open UniversityMaidan GarhiNew Delhi 110068India Department of Zoology Acharya Narendra Dev CollegeUniversity of DelhiKalkaJiNew Delhi 110019India

Objective:To formulate silver nanocomposites from achyranthes aspera leaf extracts and evaluate its larvicidal activity against Aedes ***:The silver nanocomposites were synthesized from achyranthes aspera leaf *** process was optimized and traced through UV-visible and photon correlation *** larvicidal potential of silver nanocomposites of achyranthes aspera leaf extracts was assessed against the early fourth instars of Aedes aegypti and three non-target ***,the most effective and eco-safe nanocomposite was characterized by different biophysical techniques including scanning electron microscopy(SEM),energy dispersive X-ray(EDX)spectroscopy,transmission electron microscopy(TEM),X-ray diffraction(XRD)and Fourier transform-infrared spectroscopy(FT-IR).Results:The formulated silver nanocomposites exhibited efficient larvicidal efficacy against Aedes *** with silver nanocomposites formulated using different Ag NO3 concentrations(3,4,and 5 m M)revealed respective LC50 values of 37.570,6.262 and 1.041μg/m L;5.819,1.412 and 0.489μg/m L;and 5.519,1.302 and 0.267μg/m L after 24,48 and 72 *** silver nanocomposites with 4 m M Ag NO3 were selected for *** and TEM analysis revealed spherical,poly-dispersed structure with varied diameters of 1-25 *** XRD analysis established the crystalline and face-centred-cubic structure of silver nanocomposites with the maximum peak at a 2θvalue of 37.42°.The EDX pattern showed the presence of Ag,O and C in the nanocomposites in their order of weight%.The FT-IR displayed visibly distinct peaks in different ranges demonstrating the intricacy of silver *** addition,the lethal concentrations of silver nanocomposites of achyranthes aspera leaf extracts against Aedes aegypti larvae were non-toxic to non-target organisms including Gambusia affinis,Daphnia magna and Moina ***:Silver nanocomposites synthesized with leaf extract of Achy

关键词： achyranthes aspera Aedes aegypti Larvicidal Silver nanocomposites SEM-TEM EDX XRD FT-IR

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Gastroprotective effect of achyranthes aspera Linn,leaf on rats

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Asian Pacific Journal of Tropical Medicine 2012年第3期5卷 197-201页

作者： Ashish K Das Papiya Bigoniya Neelesh K Verma AC Rana Department of Pharmacology Radharaman College of PharmacyBhopal-02M.P.India Rayat College of Pharmacy Rail Majranear Ropar NawanshahrPunjabIndia

Objective:The study was aimed al evaluating the antiulcer activity of ethanolic extract of achyranthes aspera(EEAA) ***:The anti-ulcer assays were performed on pylorus ligation and chronic ethanol induced ulcer *** effects of the EEAA on gastric content volume,*** acidity,total acidity and ulcer index were ***:The percentage of ulcer protection(59.55%and 35.58%) was significantly(P < 0.001) higher in the groups treated with the high dose of EEAA(600 mg/kg),it also reduced the volume of gastric juice and total acidity whereas,gastric pH was increased ***:The results of this study show significant gastroprotective activity of EEAA may be due to presence of phyto-constituents like flavanoids,saponins and tannins.

关键词： Antiulcer achyranthes aspera Gastroprotective Omeprazole Ethanol

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In vitro callus induction and plantlet regeneration of achyranthes aspera L.,a high value medicinal plant

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Asian Pacific Journal of Tropical Biomedicine 2014年第1期4卷 40-46页

作者： Monokesh Kumer Sen Shamima Nasrin Shahedur Rahman Abu Hena Mostofa Jamal Department of Biotechnology and Genetic Engineering Islamic University Plant Biotechnology Division National Institute of Biotechnology Department of Genetic Engineering and Biotechnology Jessore Science and Technology University

Objective:To study callus induction from different explants(internode,leaf,root)and in vitro plantlets propagation from medicinally important plant achyranthes aspera ***:Sterilized explants were prepared by uning 0.1%HgCl_2 and 0.5%Bavistin and callus was obtained when cultured onto Murashige Skoog's(MS)medium by using different concentrations and combination of 2,***,IAA,IBA with 3%sucrose and 0.8%*** callus was immediately transferred to MS medium containing at different concentrations of phytohormones for shootlets and rootlets induction ***:Sterilization treatment of 0.1%HgCl_*** 2-3 min and Bavistin 0.5%for 10-12 min showed the highest percentage of asepsis and survival *** induction of callus was obtained from a combination of 2.0 mg/L 2,4-D and 0.5 mg/L NAA from *** shootlets number(4.83±0.l7)and length(3.8±0.16)cm were observed on full strength MS medium when fortified with BAP 4.0 mg/L and KIN 0.5 mg/*** efforts of BAP 10 mg/L and NAA 0.5 mg/L on full strength MS medium showed highest leaf number(6.77±0.94).In vitro raised shoots were allowed to root on different strengths of MS medium fortified with IAA and IBA at different ***,3.0 mg/L IBA was enabled to induce maximum rootlets number(10.0±9.82)on full strength MS ***,regenerated shoots with well developed roots were successfully subjected to hardening process and were *** survived plantlets showed 66.67%survival frequency without any morphological ***:The results demonstrated that different explants were good source of callus induction,morphology analysis as well as indirect plantlets regeneration.

关键词： achyranthes aspera Callus induction Plantlets propagation Murashige Skoog’s Micropropagation

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In vitro clonal propagation of achyranthes aspera L. and achyranthes bidentata Blume using nodal explants

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Asian Pacific Journal of Tropical Biomedicine 2012年第1期2卷 1-5页

作者： Wesely Edward Gnanaraj Johnson Marimuthu Mohanamathi RB Kavitha Marappampalyam Subramanian Department of Botany Arignar Anna Government Arts College Department of Plant Biology and Plant Biotechnology St. Xavier's College(Autonomous)Palayamkottai Centre for Biotechnology Research Department of BiotechnologyMuthayammal College of Arts & Science

Objective:To develop the reproducible in vitro propagation protocols for the medicinally important plants viz.,achyranthes aspera(***)*** achyranthes bidentata(***)Blume using nodal segments as ***:Young shoots of *** and *** were harvested and washed with running tap water and treated with 0.1%bavistin and rinsed twice with distilled *** the explants were surface sterilized with 0.1%(w/v)HgCl_2 solutions for I *** rinsing with sterile distilled water for 3-4 times,nodal segments were cut into smaller segments(1 cm)and used as the *** explants were placed horizontally as well as vertically on solid basal Murashige and Skoog(MS)medium supplemented with 3%sucrose,0.6%(w/v)agar(HiMedia,Mumbai)and different concentration and combination of 6-benzyl amino purine(BAP),kinetin(Kin),naphthalene acetic acid(NAA)and indole acetic acid(IAA)for direct ***:Adventitious proliferation was obtained from *** and *** nodal segments inoculated on MS basal medium with 3%sucrose and augmented with BAP and Kin with varied *** medium augmented with 3.0 mg/L of BAP showed the highest percentage(93.60±0.71)of shootlets formation for *** and(94.70±0.53)percentages for *** number of shoots/explants(10.60±0.36)for *** and(9.50±0.56)for *** was observed in MS medium fortified with 5.0 mg/L of *** ***,maximum mean length(5.50±0.34)of shootlets was obtained in MS medium augmented with 3.0 mg/L of Kin and for ***(5.40±0.61)was observed in the very same *** highest percentage,maximum number of rootlets/shootlet and mean length of rootlets were observed in 1/2 MS medium supplemented with 1.0 mg/L of *** percentages of plants were successfully established in *** eight percentages of plants were well established in the green house *** five percentages of plants were established in the ***:The

关键词： In vitro Plant regeneration Tissue culture Nodal culture achyranthes aspera achyranthes bidentata Clonal propagation Nodal explant Reproducible MS medium Shoots BAP NAA IAA Kinetin Nodal segment Shootlet Rootlet

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Chemical Components of achyranthes bidentata Leaves by Ultra High Performance Liquid Chromatography-Mass Spectrometry

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Medicinal Plant 2019年第4期10卷 16-19页

作者： Haiyang DONG Jinshuo MA Fulin YAN School of Pharmacy Sanquan College of Xinxiang Medical University

[Objectives] To study the chemical components and relative content of achyranthes bidentata leaves and provide a scientific basis for further development and utilization of A. bidentata leaves.[Methods] The chemical components of A. bidentata leaves were rapidly analyzed using the ultra high performance liquid chromatography-time of flight-high resolution mass spectrometry (UHPLC-TOF-MS).[Results] Thirty eight chemical compounds were identified in samples of A. bidentata leaves collected from Wen County of Henan Province, in which seven chemical compounds had the relative content higher than 5%, linoleic acid reached 25.7% and inokosterone A reached 13.8%.[Conclusions] A. bidentata leaves contain many kinds of chemical compounds. This study is expected to provide a certain basis for further extraction of linoleic acid and inokosterone A.

关键词： achyranthes bidentata leaves Ultra high performance liquid chromatography-mass spectrometry (UHPLC-MS) Chemical components Inokosterone

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Determination of Oleanolic Acid in achyranthes aspera L. of Different Production Areas by HPLC

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Medicinal Plant 2018年第5期9卷 10-12页

作者： Liuping WANG Shenggao YIN Yute ZHONG Huan ZHANG Lu YING Guangxi University of Chinese Medicine

[Objectives] To determine the content of oleanolic acid in 10 batches of achyranthes aspera L. from different production areas,and to establish a high performance liquid chromatography( HPLC) method for the determination of oleanolic acid in A. aspera. [Methods]Agilent C18 liquid chromatography column( 250 mm × 4. 6 mm,5 μm) was used for gradient elution with acetonitrile∶ water = 61∶ 39 as the mobile phase. The flow rate was 1 mL/min,the detection wavelength was 210 nm,and the column temperature was 35℃. [Results] The oleanolic acid injection volume showed a good linear relationship with the peak area in the range of 0. 382-7. 640 μg. The linear equation of oleanolic acid was: A = 530. 76 C,R = 1. 000 0; the range of sample recovery rate was 96. 03%-102. 73%,and the RSD value was 2. 16%( n =9). The content of the oleanolic acid was the highest in A. aspera produced in Guilin City of Guangxi( 0. 92%),and the lowest oleanolic acid content was in sample of Sitang in Nanning City of Guangxi( 0. 27%). It is recommended that the oleanolic acid content of A. aspera should not be lower than 0. 20%. [Conclusions]The HPLC method is accurate,reliable,easy to operate,and has good resolution. It is suitable for the determination of the content of oleic acid in A. aspera. It can provide reference for the quality control and standard drafting of A. aspera.

关键词： achyranthes aspera L. Oleanolic acid High performance liquid chromatography (HPLC) Content determination

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Effects of Pb on Rhizosphere Soil Enzyme Activity and Chemical Constituents of achyranthes bidentata Blume

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Medicinal Plant 2020年第1期11卷 73-75页

作者： Xinmin WANG Zhihua WEI Lihong LI Qingmei QIAO Henan University of Animal Husbandry and Economy Zhengzhou 450046China

[Objectives]The purpose of this study was to investigate the effects of Pb on rhizosphere soil enzyme activity and chemical constituents of achyranthes bidentata Blume.[Methods]*** Blume plants were cultivated in self-made polyvinyl chloride(PVC)*** soil was added with different levels of Pb(0,200,400,600,800 and 1000 mg/kg air-dried soil)to investigate the effects of Pb on dry mass,active ingredients(oleanolic acid and ecdysterone)and rhizosphere soil enzyme activity of *** Blume.[Results]The root dry mass of *** Blume cultivated in the soil with Pb level above 400 mg/kg significantly *** Pb residues in the *** *** growing in the soil with Pb level below 400 mg/kg complied with national *** contents of oleanolic acid and ecdysterone in *** Blume growing in the soil with Pb level above 600 mg/kg declined *** the Pb level of 1000 mg/kg,the activity of urease was inhibited *** activity of phosphatase was inhibited in the presence of Pb in the *** activity of sucrase was activated in the soil with Pb level below 400 mg/kg,and was inhibited in the soil with Pb level above 400 mg/kg.[Conclusions]This study has important guiding significance for the reasonable selection of planting base for *** Blume and the guarantee of its yield and quality.

关键词： achyranthes bidentata Blume Pb Soil enzyme Urease Sucrase Phosphatase

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Determination on Heavy Metals Content of achyranthes bidentata Blume. through Inductively Coupled Plasma Mass Spectrometry (ICP-MS)

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Agricultural Science & Technology 2008年第5期9卷 145-149页

作者：李宇伟王新民连瑞丽魏志华介晓磊陈士林郑州牧业工程高等专科学校河南郑州450011 中国医学科学院中国协和医科大学药用植物研究所北京100094

[Objective] The inductively coupled plasma mass spectrometry(ICP-MS)was constructed to determine the contents of lead,cadmium,mercury and arsenic in Archyranthes bidentata Blume.[Method]Under the optimum operation condition of ICP-MS,the samples were digested by *** element 114In was taken as an internal standard element to compensate body effect and ICP-MS method was used to determine the contents of lead,cadmium,mercury and arsenic.[Result]For the determined elements,the correlation coefficient(r)of standard curve was over 0.9995 and recovery rate was from 96.7% to 106.4% while RSD was less than 11.2%.The result of determination showed that the heavy metal content in Archyranthes bidentata *** standard was serious.[Conclusion]The constructed ICP-MS method with simple operation,rapid response,accuracy and high sensitivity in this experiment could be used for quality control of Chinese medicinal materials by detecting heavy metal contents in different Chinese medicinal materials from original places.

关键词： Microwave digestion Inductively coupled plasma mass spectrometry(ICP-MS) achyranthes bidentata Blume. Heavy metal

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