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Detection of acetyl-coa carboxylase （ACCase） Inhibitor Herbicides Resistance in Sterile Wild Oat （Avena sterilis L.） Using Agar Quick Test

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Journal of Agricultural Science and Technology(A) 2018年第1期8卷 10-17页

作者： Abdullatief Mohammed Abdurruhman Sibel Uygur and Feyzullah Nezihi Uygur Department of PlantProtection Faculty of AgricultureCukurova UniversityAdana1330Turkey

This study was conducted using seeds in Petri dish containing agar medium in order to determine acetyl-coa carboxylase （ACCase） herbicides resistance （R） in Avena sterilis that was grown in wheat fields at Adana province, Turkey. Seeds were collected from one large suspected field, where clodinafop-propargyl （Aryloxyphenoxypropionate “FOPs”） and pinoxaden （Phenylpyrazoline “DEN”） have been applied for many years. Susceptible （S） population was collected from the road side on the same region. Agar media of concentration 14 g/L was prepared and it was melted in microwave. Then the amount of 20 mL agar media was added into each Petri dish. Five seeds were placed on agar mediums containing discriminating dose of clodinafop and pinoxaden. Petri dishes were placed in growth incubator operating at 10 °C. After 15 d, both radicle and hypocotyl length were measured. The percentage of germinated seed and dose-response curves were determined. At these different concentration levels, there were more than 50% of R and less than 40% of S seed germinated for pinoxaden. However, for clodinafop, more than 60% of R and less than 50% of S seeds were germinated. At higher concentration levels, the populations of resistant and susceptible were not germinated for both herbicides. The resistance value of R population was then compared with that of the S biotype. From the resistance index （RI）, the population was more resistant to pinoxaden （7.43 for radicle and 2.47 for hypocotyl） than the clodinafop-propagyl （1.39 for radicle and 3.77 for hypocotyl）. The method provided a simple, quick and cost effective way to identify ACCase herbicides resistance in most grass weeds.

关键词： acetyl-coa carboxylase herbicides resistance Avena sterilis L. clodinafop-propargyl pinoxaden.

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Cloning, Expression and Purification of Wheat acetyl-coa carboxylases CT Domain in E. coil

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Chemical Research in Chinese Universities 2008年第6期24卷 752-755页

作者： WANG Rui-jian YANG Xue-ying ZHENG Liang-yu YANG Ye GAO Gui CAO Shu-gui Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin University Changchun 130021 P. R. China College of Forestry Beihua University Jilin 132013 P. R. China

The entire gene of carboxyltransferase（CT） domain of acetyl-coa carboxylase（ACCase） from Chinese Spring wheat（CSW） plastid was cloned firstly, and the 2.3 kb gene was inserted into PET28a^＋ vector and expressed in E. coil in a soluble state. The （His）6 fusion protein was identified by SDS-PAGE and Western blot. The recombinant protein was purified by affinity chromatography, and the calculated molecular mass（Mr） was 88000. The results of the sequence analysis indicate that the cloned gene（GeneBank accession No. EU124675） was a supplement and revision of the reported ACCase CT partial cDNA from Chinese Spring wheat plastid. The recombinant protein will be significant for us to investigate the recognizing mechanism between ACCase and herbicides, and further to screen new herbicides.

关键词： Chinese Spring wheat acetyl-coa carboxylase Carboxyltransferase(CT) domain Cloning Expression Purification

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acetyl coenzyme A carboxylase modulates lipogenesis and sugar homeostasis in Blattella germanica

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Insect Science 2024年第2期31卷 387-404页

作者： Xiaojin Pei Tiantian Bai Yuan Luo Zhanfeng Zhang Sheng Li Yongliang Fan Tong-Xian Liu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology and Institute of Insect Science and Technology School of Life SciencesSouth China Normal UniversityGuangzhouChina State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau Ministry of AgricultureNorthwest A&F UniversityYanglingShaanxiChina Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhouGuangdong ProvinceChina Institute of Entomology Guizhou UniversityGuiyangChina

Lipid and sugar homeostasis is critical for insect development and *** this study,we characterized an acetyl coenzyme A carboxylase gene in Blattella germanica(BgACC)that is involved in both lipogenesis and sugar *** found that BgACC was dominantly expressed in the fat body and integument,and was significantly upregulated after *** of BgACC in 5th-instar nymphs did not affect their normal molting to the next nymphal stage,but it caused a lethal phenotype during adult ***-RNA interference(RNAi)significantly downregulated total free fatty acid(FFA)and triacylglycerol(TAG)levels,and also caused a significant decrease of cuticular hydrocarbons(CHCs).Repression of BgACC in adult females affected the development of oocytes and resulted in sterile females,but BgACC-RNAi did not affect the reproductive ability of ***,knockdown of BgACC also changed the expression of insulin-like peptide genes(BglLPs),which mimicked a physiological state of high sugar *** addition,BgACC was upregulated when *** were fed on a high sucrose diet,and repression of BgACC upregulated the expression of the glycogen synthase gene(BgGlyS).Moreover,BgACC-RNAi increased the circulating sugar levels and glycogen storage,and a longevity assay suggested that BgACC was important for the survival of *** under conditions of high sucrose *** results confirm that BgACC is involved in multiple lipid biogenesis and sugar homeostasis processes,which further modulates insect reproduction and sugar *** study benefits our understanding of the crosstalk between lipid and sugar metabolism.

关键词： acetyl-coa carboxylase German cockroach lipid biosynthesis sugar tolerance

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The Ca^(2+)/CaN/ACC and cAMP/PKA/HK signal pathways are required for PBAN-mediated sex pheromone biosynthesis in Conogethes punctiferalis

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Journal of Integrative Agriculture 2024年第8期23卷 2735-2751页

作者： Yao Zhang Zelong She Ruolan He Shuangyan Yao Xiang Li Xiaoguang Liu Xinming Yin Jizhen Wei Mengfang Du Shiheng An State Key Laboratory of Wheat and Maize Crop Science/Henan International Laboratory for Green Pest Control/College of Plant Protection Henan Agricultural UniversityZhengzhou 450046China

Conogethes punctiferalis is a crop and fruit pest that has caused serious economic losses to agricultural *** pest relies heavily on its sex pheromone to ensure sexual encounters and subsequent mating ***,the molecular mechanism underlying sex pheromone biosynthesis in this species remains *** present study investigated the detailed mechanism underlying PBAN-regulated sex pheromone biosynthesis in *** by transcriptome sequencing of the *** pheromone glands(PGs)and subsequent functional identification of the target *** results showed that female mating started from the first scotophase,and peaked at the second to fifth scotophases in accordance with the release of sex *** regulated sex pheromone biosynthesis by employing Ca^(2+)and cAMP as secondary messengers,as demonstrated by RNA interference(RNAi),pharmacological inhibitors,and behavioral *** investigation revealed that calcineurin(CaN)and acetyl-coa carboxylase(ACC)were activated by PBAN/Ca^(2+)signaling,and the RNAimediated knockdown of CaN and ACC transcripts significantly reduced sex pheromone production,ultimately leading to a significantly reduced ability of females to attract ***,hexokinase(HK)was found to regulate sex pheromone biosynthesis in response to the PBAN/cAMP/PKA signaling pathway,as demonstrated by RNAi,enzyme activity,and pharmacological inhibitor ***,Far2 and Desaturase1 were found to participate in PBAN-regulated sex pheromone ***,our findings revealed that PBAN regulates sex pheromone biosynthesis through the PBANR/Ca^(2+)/CaN/ACC and PBANR/cAMP/PKA/HK pathways in ***,which enriches our comprehension of the details of sex pheromone biosynthesis in moths.

关键词： Conogethes punctiferalis sex pheromone cAMP/PKA calcineurin acetyl-coa carboxylase hexokinase

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Roles of vitamin A in the regulation of fatty acid synthesis

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World Journal of Clinical Cases 2021年第18期9卷 4506-4519页

作者： Fu-Chen Yang Feng Xu Tian-Nan Wang Guo-Xun Chen Jiangsu Food and Pharmaceutical College Huaian 223003Jiangsu ProvinceChina Department of Urology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Department of Nutrition The University of TennesseeKnoxvilleTN 37909United States

Dietary macronutrients and micronutrients play important roles in human *** the other hand,the excessive energy derived from food is stored in the form of triacylglycerol.A variety of dietary and hormonal factors affect this process through the regulation of the activities and expression levels of those key player enzymes involved in fatty acid biosynthesis such as acetyl-coa carboxylase,fatty acid synthase,fatty acid elongases,and *** a micronutrient,vitamin A is essential for the health of ***,vitamin A has been shown to play a role in the regulation of glucose and lipid *** review summarizes recent research progresses about the roles of vitamin A in fatty acid *** focuses on the effects of vitamin A on the activities and expression levels of mRNA and proteins of key enzymes for fatty acid synthesis in vitro and in *** appears that vitamin A status and its signaling pathway regulate the expression levels of enzymes involved in fatty acid *** research directions are also discussed.

关键词： Vitamin A acetyl-coa carboxylase Fatty acid synthase Fatty acid elongase Stearoyl-coa desaturase Fatty acid synthesis

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Fanlian Huazhuo Formula:A promising herbal preparation for metabolic liver disease

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World Journal of Gastroenterology 2024年第46期30卷 4964-4968页

作者： Bhupesh Singla Pharmaceutical Sciences The University of Tennessee Health Science CenterMemphisTN 38103United States

The prevalence of metabolic dysfunction-associated steatotic liver disease(MASLD)has increased significantly in recent decades and is projected to increase further due to the rising obesity *** patients are at higher risk of developing advanced liver diseases“cirrhosis and hepatocellular carcinoma”as well as liver-or cardiovascular-related *** lipid-lowering therapies failed to reduce the risk of mortality in these ***,there is an urgent need for pharmacotherapies that can control and even reverse this *** Huazhuo Formula(FLHZF)is a combination herbal preparation,and its various individual constituents regulate hepatic lipid metabolism,adipose tissue inflammation,and gut ***,these useful effects,limited information is available on its benefits in diet-induced *** this article,we discuss the research findings recently published about the therapeutic effects of FLHZF in suppressing MASLD development and underlying *** a series of in vitro and in vivo experiments,the authors demonstrated for the first time that FLHZF suppresses MASLD in male mice possibly by inhibiting hepatic de novo lipogenesis pathways and reducing hepatocyte *** study paves the way for future investigations aimed at investigating FLHZF’s role in inhibiting lipogenesis particularly using radioactively-labeled glucose and acetate,and governing hepatocyte mitochondrial function,gut microbiome profile,and its effects in other models of MASLD,and female mice.

关键词： Metabolic dysfunction-associated steatotic liver disease Fanlian Huazhuo Formula De novo lipogenesis Cell death Adenosine-5’-monophosphate-activated protein kinase alpha acetyl-coa carboxylase

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