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Effects of acetosyringone on Genetic Transformation of Waxy Maize Using Different Receptor Systems

Agricultural Biotechnology 2013年第5期2卷 1-3,8页

作者： Xinmei GUO Xinming CHE Yuhe PEI Xiyun SONG College of Agronomy and Plant Protection Qingdao Agricultural University Shandong Key Laboratory of Maize Breeding and Cultivation Techniques/Shandong Denghai Seeds Co. Ltd.

acetosyringone （AS） has great influences on Agrobacterium-mediated genetic transformation. In order to improve the transformation frequency of waxy maize, in this study, shoot tips and immature embryo-derived callus of Lainongnuo 38 were collected as receptor materials, to compare the effects of pH in suspension medium, addition modes and concentrations of acetosyringone on genetic transformation of waxy maize. Results showed that adding 2 μl of 150 μmol/L AS in the wound with suspension medimn at pH 5.2 can significantly improve the transformation frequency of shoot tips of waxy maize; in genetic transformation of maize callus, adding 5 mg/L AS in infection solution can significantly improve the percentage of resistant callus.

关键词： Receptor system acetosyringone Addition mode Transformation frequency

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Rapid delivery of Cas9 gene into the tomato cv.‘Heinz 1706’through an optimized Agrobacterium-mediated transformation procedure

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BIOCELL 2021年第1期45卷 199-215页

作者： BEEMNET MENGESHA KASSAHUN BEUM-CHANG KANG SU-JI BAE YE JIN NAM GRETEL FONSECA MUNDO GA-HUI KANG KYOUNGOOK KIM JEUNG-SUL HAN Department of Horticulture Kyungpook National UniversityDaegu41566Korea Center for Genome Engineering Institute for Basic ScienceDaejeon34047Korea School of Water Energyand EnvironmentCranfield UniversityCranfieldMK430ALUK Institute of Agricultural Science and Technology Kyungpook National UniversityDaegu41566Korea

Solanum lycopersicum‘Heinz 1706’is a pioneer model cultivar for tomato research,whose whole genome sequence valuable for genomics studies is ***,a genetic transformation procedure for this cultivar has not yet been ***,various genome editing technologies such as transfection of clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas)ribonucleoprotein complexes into cells are in the *** the Cas9-expressing genotype possessing a reference genome can simplify the verification of an off-target effect,resolve the economic cost of Cas9 endonuclease preparation,and avoid the complex assembly process together with single-guide RNA(sgRNA)in the transfection ***,this study was designed to generate Cas9-expressing‘Heinz 1706’lines by establishing an Agrobacterium tumefaciens-mediated transformation(ATMT)***,we report a rapid and reproducible transformation procedure for‘Heinz 1706’by finetuning various factors:*** strain,pre-culture and co-culture durations,a proper combination of phytohormones at each step,supplementation of acetosyringone,and shooting/rooting ***,through eluding subculture and simultaneously inducing shoot elongation and rooting from leaf cluster,we achieved a short duration of three months for recovering the transgenic plants expressing *** presence of the Cas9 gene and its stable expression were confirmed by PCR and qRT-PCR analyses,and the Cas9 gene integrated into the T_(0) plant genome was stably transmitted to T_(1) ***,we anticipate that our procedure appears to ease the conventional ATMT in‘Heinz 1706’,and the created Cas9-expressing‘Heinz 1706’lines are ultimately useful in gene editing via unilateral transfection of sgRNA into the protoplasts.

关键词： Transgenic plant Phytohormone acetosyringone Gene editing

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PHENOLIC COMPOUNDS CAN PROMOTE THE TRANSFORMATION OF MONOCOTYLEDONOUS Commelina communis BY Agrobacterium tumefaciens

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Chinese Science Bulletin 1989年第21期34卷 1815-1819页

作者：邓万银邵启全 Institute of Genetics Academia Sinica Beijing 100012 PRC

Agrobacterium tumefaciens can infect wounded plant cells, transfer a part of its Ti plasmid (T-DNA) into plant genomes and cause crown gall tumors. At present the Ti plasmid of A. tumefaciens is the best vector availa... 详细信息

关键词： Commelina cammunis transformation acetosyringone

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Agrobacterium tumefaciens CAN TRANSFORM Triticum aestivum AND Hordeum vulgare OF GRAMINEAE

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Science China Chemistry 1990年第1期33卷 27-34页

作者：邓万银林晓影邵启全 Institute of Genetics Academia Sinica Beijing 100012 PRC

So far there is no suitable vector system for the genetic engineering of monocotyledonous plants, especially cereal crops. It is first reported in this paper that Agrobacterium tumefaciens strains T37, A208 and B6 can transform some varieties of Triticum aestivum and Hordeum vulgare to form swellings and tumors. Itis shown in the experiments that phenolic compound acetosyringone may promote the transformation process. In addition, inoculating agrobacteria on appropriate plant tissues is also proved to be the key step in achieving successful transformation. The host range of A. tumefaciens and the possibility of utilizing Ti plasmid as the vector for the gene transfer of cereal crops are discussed.

关键词： Agrobacterium tumefaciens Triticum aestivum Hordeum vulgarz acetosyringone transformation of cereal crops.

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二妙丸HPLC指纹图谱及UPLC-LTQ-Orbitrap成分分析

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中草药 2017年第17期48卷 3511-3516页

作者：雷蓉周亚楠刘永利冯丽河北省药品检验研究院河北石家庄050011

目的建立二妙丸的HPLC指纹图谱质量评价方法,并对二妙丸中的化学成分进行UPLC-LTQ-Orbitrap定性分析。方法指纹图谱采用HPLC法,色谱柱为Shiseido C18柱(250 mm×4.6 mm,5μm),乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,体积流量为1.0 m ... 详细信息

目的建立二妙丸的HPLC指纹图谱质量评价方法,并对二妙丸中的化学成分进行UPLC-LTQ-Orbitrap定性分析。方法指纹图谱采用HPLC法,色谱柱为Shiseido C18柱(250 mm×4.6 mm,5μm),乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,体积流量为1.0 m L/min,检测波长为330 nm,以黄柏、苍术混合对照药材生成的色谱图为标准对照图谱,采用"中国药典委员会相似度评价软件(2012A)"对样品进行相似度评价;采用UPLC-LTQ-Orbitrap技术对二妙丸中化学成分进行定性分析,色谱柱为Thermo Scientific Syncronis C18柱(100 mm×2.1 mm,1.9μm),乙腈-0.1%甲酸水溶液梯度洗脱,经DAD检测器后采用LTQ-Orbitrap Elite质谱正离子模式采集,各成分的一级全扫描离子在离子阱内进行CID多级质谱、FT检测高分辨质谱数据分析。结果所建立的指纹图谱有10个共有色谱峰,20批样品的相似度在0.869~0.992。通过对照品指认、文献比对以及高分辨质谱数据解析,鉴定了21个化学成分,分别为neo-chlorogenic acid、木兰箭毒碱、竹叶椒碱、木兰花碱、3-O-feruloylquinic acid、蝙蝠葛碱、demethyleneberberine、oxyberberine、非洲防己碱、药根碱、小檗红碱、巴马汀、小檗碱、丁香酸、咖啡酸、(E)-4-(3-hydroxyprop-1-en1yl)-2-methoxyphenol、白术内酯II、acetosyringone、白术内酯I、selina-4(14),7(11)-dien-8-one、苍术素。结论建立的二妙丸HPLC指纹图谱专属性强,重现性好,结合液质联用技术的定性分析,可全面评价二妙丸的内在质量。

关键词：二妙丸指纹图谱 HPLC UPLC LTQ-Orbitrap 质量评价成分分析相似度评价 neo-chlorogenic acid 木兰箭毒碱竹叶椒碱木兰花碱 3-O-feruloylquinic acid 蝙蝠葛碱 demethyleneberberine oxyberberine 非洲防己碱药根碱小檗红碱巴马汀小檗碱丁香酸咖啡酸 (E)-4-(3-hydroxyprop-1-en1yl)-2-methoxyphenol 白术内酯Ⅱ acetosyringone 白术内酯Ⅰ selina-4(14),7(11)-dien-8-one 苍术素

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Effects of Acetylacetone Solution Soaking on Agrobacterium - transformed Maize Seed Buds

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Agricultural Biotechnology 2021年第3期10卷 11-14页

作者： Mengyu LYU Fushuang DONG Shuo ZHOU Jianfang CHAI He ZHAO Yongwei LIU Fan YANG Bo JIAO Xiaoyu LI Junmin ZHANG Haibo WANG Plant Genetic Engineering Center of Hebei Province Institute of Genetics and PhysiologyHebei Academy of Agriculture and Forestry Sciences(Agricultural Product Quality and Safety Research Center of Hebei Academy of Agricultural and Forestry Sciences)Shijiazhuang 050051China

[Objectives]The effect of acetosyringone seed soaking on the transformation of maize seed buds was analyzed,so as to improve the genetic transformation efficiency of maize and to provide technical support for transgenic breeding of maize.[Methods]The seeds of the"Zheng 58"maize inbred line were used as experimental *** the seeds were germinated,AS was added to the water at concentrations of 70,140,210,and 280μmol/L,respectively,and the seeds germinated without the addition of AS served as the *** Agrobacterium-mediated method was used to transform bud growth points of maize seeds,and green fluorescent protein detection was performed on the young shoots transformed with EGFP(enhanced green fluorescent protein)*** effect of soaking seeds with acetosyringone solution on the transformation of maize bud growth points by Agrobacterium was studied according to the detection results.[Results]Soaking seeds in acetosyringone solutions for germination had the effect of inhibiting the germination of maize seeds and inhibiting sprout elongation,and the higher the concentration of acetosyringone,the stronger the *** the concentration of acetosyringone solution was 280μmol/L,the germination rate of seeds was only 36.2%of the CK,while soaking seeds with 70-140μmol/L acetosyringone solution for germination could not only ensure a higher germination rate of maize seeds,but also significantly increased the transformation efficiency of maize bud growth *** the seeds were soaked with 70μmol/L acetosyringone solution for germination,the positive rate of transformed maize buds was the highest,reaching 32.1%.[Conclusions]When maize bud growth points were used as the receptor of Agrobacterium transformation,soaking seeds with 70-140μmol/L acetosyringone for germination basically did not affect the germination of seeds,and was beneficial to the activation of Agrobacterium,thereby promoting the transformation.

关键词： Maize acetosyringone Seed soaking Transgenic efficiency

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Efficient method of Agrobacterium tumefaciens-mediated transformation for Casuarina cunninghamiana using seedling explants

Efficient method of Agrobacterium tumefaciens-mediated trans...

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第九届中国林业青年学术年会

作者： D.Bogusz C.Franche Equipe Rhizogenèse UMR DIAPCInstitut de Recherche pour le Développement（IRD）

＜正＞Casuarina cunninghamiana Miq is one of the largest tree species of the genus Casuarina, the angiosperm family Casuarinaceae comprising four distinct genera and about 96 species native to Australia,Southeast Asia and Islands of Western Pacific *** cunninghamiana is a pioneer tree species of multipurpose uses from environmental protection to fuelwood production. The species has high ability to grow vigorously on poor coastal sandy and saline *** capacity of rapid growth was mostly contributed from a symbiotic association with an actinomycete of the genus Frankia,which allows C cunninghamiana to fix dinitrogen in root nodules,so-called *** there is limited knowledge about the biochemistry and molecular biology of the infection process and nodule formation in actinorhizal *** on gene transformation can explore the regulation of plant symbiotic gene expression,and better understand of the evolution of symbiotic associations and *** genetic improvement.

关键词： Casuarina cunninghamiana Agrobacterium tumefaciens Genetic transformation P-Glucuronidase acetosyringone Transgenic plants

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