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RAPD analysis of natural populations of acanthopanax brachypus

Cell Research 1997年第1期7卷 99-106页

作者： YAN HUA JUN SI LAN DAI NAI HU WU(Institute of Developmental Biology, Chinese Academy of Sciences, Beiling 100080) Institute of Developmental Biology Chinese Academy of Sciences Beijing 100080&#x0002A This paper is specially dedicated to Professor Lu Ji SHI on the 80th anniversary of his birthdayReceived 31March1997 Revised 23May1997 Accepted 2June1997

Random Amplified Polymorphic DNA (RAPD) analysis is a new technology of molecular marking which has proved very powerful in detecting genetic diversity at the level of population. The genomic DNAs used in our experiment were extracted from fresh leaves taken from 59 individuals sampled from three natural populations in Yan An, Shanxi Province. Through more than 2,000 PCRs, deep-going RAPD analysis was carried out on DNA samples from 49 inviduals. The percentage of polymorphic RAPD loci found in these three populations were respectively 27.2 %, 18.6 % and 5.4 %; the average genetic distances within population, 0.055, 0.036 and 0.008; the average genetic distances between populations (Ⅰ-Ⅱ), (Ⅰ-Ⅲ) and (Ⅱ-Ⅲ), 0.105, 0.096 and 0.060. The genetic diversity of A. brachypus within and between populations was found, for the first time, to be rather poor,thus revealing innate factors as the cause contributing to its endangered status. In addition, our work also provides basic materials for elucidating the underlying cause of its endangerment and for its protection biology.

关键词： acanthopanax brachypus genetic diversity random amplified polymorphic DNA(RAPD) analysis

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